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However, the role of let-7a-5p in chronic rhinosinusitis with nasal polyps (CRSwNP) has not been reported. This study is designed to determine the pattern of expression and role of let-7a-5p in CRSwNP.<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Methods<\/jats:title>\n                <jats:p>The expression level of let-7a-5p, TNF-\u03b1, IL-1\u03b2, and IL-6 in CRSwNP tissues and cells were detected by RT-qPCR. Western blot assay was carried out to measure the protein expression of the Ras-MAPK pathway. Dual luciferase reporter assay and RNA pull-down assay were used to explore the relationship between let-7a-5p and IL-6.<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Results<\/jats:title>\n                <jats:p>Let-7a-5p was significantly downregulated in CRSwNP tissues and cells. Moreover, the mRNA expression of TNF-\u03b1, IL-1\u03b2 and IL-6 was increased in CRSwNP tissues, while let-7a-5p mimic inhibited the expression of TNF-\u03b1, IL-1\u03b2 and IL-6. Besides that, let-7a-5p was negatively correlated with TNF-\u03b1, IL-1\u03b2 and IL-6 in CRSwNP tissues. In our study, IL-6 was found to be a target gene of let-7a-5p. Additionally, let-7-5p mimic obviously reduced the protein levels of Ras, p-Raf1, p-MEK1 and p-ERK1\/2, while IL-6 overexpression destroyed the inhibitory effect of let-7a-5p on the Ras-MAPK pathway in CRSwNP.<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Conclusion<\/jats:title>\n                <jats:p>We demonstrated that let-7a-5p\/IL-6 interaction regulated the inflammatory response through the Ras-MAPK pathway in CRSwNP.<\/jats:p>\n              <\/jats:sec>","DOI":"10.1186\/s13000-021-01089-0","type":"journal-article","created":{"date-parts":[[2021,3,30]],"date-time":"2021-03-30T09:02:46Z","timestamp":1617094966000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":26,"title":["Let-7a-5p regulates the inflammatory response in chronic rhinosinusitis with nasal polyps"],"prefix":"10.1186","volume":"16","author":[{"given":"Jianwei","family":"Zhang","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lei","family":"Han","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Feng","family":"Chen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2021,3,30]]},"reference":[{"key":"1089_CR1","doi-asserted-by":"crossref","unstructured":"Khan A, Vandeplas G, Huynh TMT, Joish VN, Bachert C. 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