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For high quality, Sichuan Zhongjiang is regarded as the genuine producing area of\n                      <jats:italic>S. miltiorrhiza.<\/jats:italic>\n                      Given its abnormal pollen development,\n                      <jats:italic>S. miltiorrhiza<\/jats:italic>\n                      from Sichuan (\n                      <jats:italic>S.m.<\/jats:italic>\n                      -SC) relies on root reproduction and zymad accumulation; part of diseased plants present typical viral disease symptoms and seed quality degeneration. This study aim to detected unknown viruses from mosaic-diseased plants and establish a highly efficient virus-free regeneration system to recover germplasm properties.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      Tobacco mosaic virus (TMV) and cucumber mosaic virus (CMV) were detected from mosaic-diseased plants. Primary apical meristem with two phyllo podium in 0.15\u20130.5\u00a0mm peeled from diseased plants were achieved 73.33% virus-free rate. The results showed that the medium containing MS, 0.5\u00a0mg\/L 6-BA, 0.1\u00a0mg\/L NAA, 0.1\u00a0mg\/L GA\n                      <jats:sub>3<\/jats:sub>\n                      , 30\u00a0g\/L sucrose and 7.5\u00a0g\/L agar can achieve embryonic-tissue (apical meristem, petiole and leaf callus) high efficient organogenesis. For callus induction, the optimal condition was detected on the medium containing MS, 2\u00a0mg\/L TDZ, 0.1\u00a0mg\/L NAA by using secondary petiole of virus-free plants under 24\u00a0h dark\/d condition for 21 d. The optimal system for root induction was the nutrient solution with 1\/2 MS supplemented with 1\u00a0mg\/L NAA. After transplant, the detection of agronomic metric and salvianolic acid B content confirmed the great germplasm properties of S.m.-SC virus-free plants.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>\n                      A highly efficient virus-free regeneration system of\n                      <jats:italic>S.m.-<\/jats:italic>\n                      SC was established based on the detected viruses to recover superior seed quality. The proposed system laid support to control disease spread, recover good germplasm properties in\n                      <jats:italic>S.m.-<\/jats:italic>\n                      SC.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1186\/s13007-022-00872-4","type":"journal-article","created":{"date-parts":[[2022,4,18]],"date-time":"2022-04-18T04:02:41Z","timestamp":1650254561000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":30,"title":["Development of a highly efficient virus-free regeneration system of Salvia miltiorrhiza from Sichuan using apical meristem as explants"],"prefix":"10.1186","volume":"18","author":[{"given":"Si Cheng","family":"Yao","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuan Yuan","family":"Jiang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Su","family":"Ni","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Long","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jun","family":"Feng","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rui Wu","family":"Yang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Li Xia","family":"Yang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qiu Yan","family":"Len","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2651-4777","authenticated-orcid":false,"given":"Li","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2022,4,18]]},"reference":[{"key":"872_CR1","doi-asserted-by":"publisher","first-page":"621169","DOI":"10.3389\/fphar.2021.621169","volume":"12","author":"KY Kum","year":"2021","unstructured":"Kum KY, Kirchhof R, Luick R, et al. 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