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The resurgence of\n                      <jats:italic>P. sojae\u00a0<\/jats:italic>\n                      (\n                      <jats:italic>Phytophthora sojae<\/jats:italic>\n                      )\u00a0is primarily ascribed to the durable oospores found in soil and remnants of the disease.\n                      <jats:italic>P. sojae<\/jats:italic>\n                      is capable of infesting at any growth periods of the soybean, and the succeed infestation of\n                      <jats:italic>P. sojae<\/jats:italic>\n                      is predominantly attributed to long-lived oospores present in soil. Comprehending the molecular mechanisms that drive oospores formation and their significance in infestation is the key for effective management of the disease. However, the existing challenges in isolating and extracting significant quantities of oospores pose limitations in investigating the sexual reproductive stages of\n                      <jats:italic>P. sojae<\/jats:italic>\n                      .\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      The study focused on optimizing and refining the culture conditions and extraction process of\n                      <jats:italic>P. sojae<\/jats:italic>\n                      , resulting in establishment of an efficient and the dependable method for extraction. Novel optimized approach was yielded greater quantities of high-purity\n                      <jats:italic>P. sojae<\/jats:italic>\n                      oospores than traditional methods. The novel approach exceeds the traditional approaches with respect to viability, survival ability, germination rates of new oospores and the pathogenicity of oospores in potting experiments.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>\n                      The proposed method for extracting\n                      <jats:italic>P. sojae<\/jats:italic>\n                      oospores efficiently yielded a substantial quantity of highly pure, viable, and pathogenic oospores. The enhancements in oospores extraction techniques will promote the research on the sexual reproductive mechanisms of\n                      <jats:italic>P. sojae<\/jats:italic>\n                      and lead to the creation of innovative and effective approaches for managing oomycete diseases.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1186\/s13007-024-01199-y","type":"journal-article","created":{"date-parts":[[2024,5,16]],"date-time":"2024-05-16T00:01:52Z","timestamp":1715817712000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["A novel method for extraction of high purity and high production Phytophthora sojae oospores"],"prefix":"10.1186","volume":"20","author":[{"given":"Xiaomeng","family":"Chu","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ziyi","family":"Yin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pengjie","family":"Yue","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xinyu","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yue","family":"Yang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiayi","family":"Sun","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ziying","family":"Kong","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jian","family":"Ren","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaohan","family":"Liu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chongchong","family":"Lu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Haipeng","family":"Zhao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yang","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xinhua","family":"Ding","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2024,5,16]]},"reference":[{"issue":"1","key":"1199_CR1","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1111\/j.1364-3703.2006.00373.x","volume":"8","author":"BM Tyler","year":"2007","unstructured":"Tyler BM. 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