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Since the growth velocity in the physis of long bones decreases during aging, we sought to evaluate the role of the physis on influencing the development of <jats:italic>Staphylococcus aureus<\/jats:italic> HOIAI in a mouse model comparing younger versus older mice.<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Methods<\/jats:title>\n                <jats:p>In a mouse model of HOIAI, a sterile Kirschner wire was inserted retrograde into the distal femur of younger (5\u20138-week-old) and older (14\u201321-week-old) mice. After a 3-week convalescent period, a bioluminescent <jats:italic>Staphylococcus aureus<\/jats:italic> strain was inoculated intravenously. Bacterial dissemination to operative and non-operative legs was monitored longitudinally <jats:italic>in vivo<\/jats:italic> for 4 weeks, followed by <jats:italic>ex vivo<\/jats:italic> bacterial enumeration and X-ray analysis.<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Results<\/jats:title>\n                <jats:p><jats:italic>In vivo<\/jats:italic> bioluminescence imaging and <jats:italic>ex vivo<\/jats:italic> CFU enumeration of the bone\/joint tissue demonstrated that older mice had a strong predilection for developing a hematogenous infection in the operative legs but not the non-operative legs. In contrast, this predilection was less apparent in younger mice as the infection occurred at a similar rate in both the operative and non-operative legs. X-ray imaging revealed that the operative legs of younger mice had decreased femoral length, likely due to the surgical and\/or infectious insult to the more active physis, which was not observed in older mice. Both age groups demonstrated substantial reactive bone changes in the operative leg due to infection.<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Conclusions<\/jats:title>\n                <jats:p>The presence of an implant was an important determinant for developing a hematogenous orthopaedic infection in older but not younger mice, whereas younger mice had a similar predilection for developing periarticular infection whether or not an implant was present. On a clinical scale, diagnosing HOIAI may be difficult particularly in at-risk patients with limited examination or other data points. Understanding the influence of age on developing HOIAI may guide clinical surveillance and decision-making in at-risk patients.<\/jats:p>\n              <\/jats:sec>","DOI":"10.1186\/s13018-021-02594-0","type":"journal-article","created":{"date-parts":[[2021,9,14]],"date-time":"2021-09-14T10:02:59Z","timestamp":1631613779000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Predilection for developing a hematogenous orthopaedic implant-associated infection in older versus younger mice"],"prefix":"10.1186","volume":"16","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7981-2882","authenticated-orcid":false,"given":"John M.","family":"Thompson","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alyssa G.","family":"Ashbaugh","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yu","family":"Wang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Robert J.","family":"Miller","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Julie E.","family":"Pickett","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Daniel L. 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