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Whether a menopause-related excess of iron contributes to the development of postmenopausal osteoporosis has remained unresolved due to a lack of an appropriate animal model. This study aimed to explore the effects of iron accumulation in bone mass in estrogen-deficient rats.<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Methods<\/jats:title>\n                <jats:p>In the present study, ovariectomy (OVX) was performed in female rats and the changes of iron metabolism and some related modulated genes were detected. Ferric ammonium citrate (FAC) was used as a donor of iron for OVX rats. Moreover, micro-CT was performed to assess the bone microarchitecture in sham group, OVX, and FAC groups. Histological detection of iron in liver was assessed by Perl\u2019s staining. The expressions of \u03b2-CTX and osteocalcin were assessed by ELISA.<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Results<\/jats:title>\n                <jats:p>It was found that serum iron decreased after OVX. It was found that the expressions of <jats:italic>Hepcidin<\/jats:italic> in liver and <jats:italic>Fpn<\/jats:italic>, <jats:italic>DMT-1<\/jats:italic> in duodenum significantly decreased at transcriptional level in OVX group than sham group. However, no difference existed in the expression of <jats:italic>DMT-1<\/jats:italic>. Then, ferric ammonium citrate (FAC) was used as a donor of iron for OVX rats. The FAC group manifested significant iron accumulation by increased serum iron and hepatic iron content. In addition, FAC treatment accelerated bone loss and decreased BMD and biomechanics in OVX rats. Moreover, bone biomarker \u03b2-CTX rather than osteocalcin increased significantly in FAC groups than OVX group.<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Conclusions<\/jats:title>\n                <jats:p>In conclusion, no iron accumulation occurred in OVX rats. Furthermore, iron accumulation could further deteriorate osteopenia through enhanced bone resorption.<\/jats:p>\n              <\/jats:sec>","DOI":"10.1186\/s13018-021-02663-4","type":"journal-article","created":{"date-parts":[[2021,8,24]],"date-time":"2021-08-24T07:02:54Z","timestamp":1629788574000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":25,"title":["Iron accumulation deteriorated bone loss in estrogen-deficient rats"],"prefix":"10.1186","volume":"16","author":[{"given":"Lu-lin","family":"Liu","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gong-wen","family":"Liu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hui","family":"Liu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kai","family":"Zhao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"You-jia","family":"Xu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2021,8,24]]},"reference":[{"issue":"2","key":"2663_CR1","doi-asserted-by":"publisher","first-page":"129","DOI":"10.1097\/MOG.0b013e32831ef1f7","volume":"25","author":"GJ Anderson","year":"2009","unstructured":"Anderson GJ, Frazer DM, McLaren GD. 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