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Each locus has been validated by polymerase chain reaction and pyrosequencing. The selected loci were analyzed by Herb molecular quantification (Herb-Q) assay to complete the quantitative methodology verification and establish a quantitative detection system for homemade licorice mixtures and one of the patent medicines\u2014Liuyi San.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>Outstanding performance was observed in quantitative validation, which evaluated linearity (0.9989), limit of detection (2%) and quantification (2%), and repeatability; additionally, a quantitative detection system was established for homemade licorice mixtures and Liuyi San (one of the patent medicines), with the lowest bias being 3.48%. When both GG and GU were present in the mixed powder, the average biases for GG and GU quantification were 8.25% and 8.01%, respectively. When only GG was present in Liuyi San, the bias was 6.43%; when both GG and GU were present, the biases for GG and GU were 5.61% and 3.48%, respectively.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>This study successfully established an accurate detection system for quantifying the botanical origin of edible-medicinal licorice, which represents a significant milestone in enhancing the safety, efficacy, and quality control in licorice products.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1186\/s13020-025-01263-2","type":"journal-article","created":{"date-parts":[[2025,11,24]],"date-time":"2025-11-24T01:02:31Z","timestamp":1763946151000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["A novel algorithm- specific loci screening accelerates the establishment of molecular quantification of Glycyrrhiza glabra, G. uralensis, and G. inflata"],"prefix":"10.1186","volume":"20","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-3638-9017","authenticated-orcid":false,"given":"Yifei","family":"Pei","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0003-2566-2203","authenticated-orcid":false,"given":"Ziyi","family":"Liu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6490-9449","authenticated-orcid":false,"given":"Wenjun","family":"Jiang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0007-9361-4392","authenticated-orcid":false,"given":"Mingyu","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0002-4342-3938","authenticated-orcid":false,"given":"Haitao","family":"Liu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0334-1225","authenticated-orcid":false,"given":"Xue","family":"Feng","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5092-854X","authenticated-orcid":false,"given":"Xiwen","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2025,11,24]]},"reference":[{"issue":"1","key":"1263_CR1","doi-asserted-by":"publisher","first-page":"72","DOI":"10.1186\/s43094-021-00223-5","volume":"7","author":"OA Adeleye","year":"2021","unstructured":"Adeleye OA, Femi-Oyewo MN, Bamiro OA, Bakre LG, Alabi A, Ashidi JS, et al. 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