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While mouse models based on overexpression of mutated transgenes have yielded key insights in mechanisms of disease, those models are subject to artifacts, including random genetic integration of the transgene, ectopic expression and non-physiological protein levels. The genetic engineering of novel mouse models using knock-in approaches addresses some of those limitations. With mounting evidence of the role played by microglia in AD, high-dimensional approaches to phenotype microglia in those models are critical to refine our understanding of the immune response in the brain.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      We engineered a novel\n                      <jats:italic>App<\/jats:italic>\n                      knock-in mouse model (\n                      <jats:italic>App<\/jats:italic>\n                      <jats:sup>SAA<\/jats:sup>\n                      ) using homologous recombination to introduce three disease-causing coding mutations (Swedish, Arctic and Austrian) to the mouse\n                      <jats:italic>App<\/jats:italic>\n                      gene. Amyloid-\u03b2 pathology, neurodegeneration, glial responses, brain metabolism and behavioral phenotypes were characterized in heterozygous and homozygous\n                      <jats:italic>App<\/jats:italic>\n                      <jats:sup>SAA<\/jats:sup>\n                      mice at different ages in brain and\/ or biofluids. Wild type littermate mice were used as experimental controls. We used\n                      <jats:italic>in situ<\/jats:italic>\n                      imaging technologies to define the whole-brain distribution of amyloid plaques and compare it to other AD mouse models and human brain pathology. To further explore the microglial response to AD relevant pathology, we isolated microglia with fibrillar A\u03b2 content from the brain and performed transcriptomics and metabolomics analyses and\n                      <jats:italic>in vivo<\/jats:italic>\n                      brain imaging to measure energy metabolism and microglial response. Finally, we also characterized the mice in various behavioral assays.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      Leveraging multi-omics approaches, we discovered profound alteration of diverse lipids and metabolites as well as an exacerbated disease-associated transcriptomic response in microglia with high intracellular A\u03b2 content. The\n                      <jats:italic>App<\/jats:italic>\n                      <jats:sup>SAA<\/jats:sup>\n                      knock-in mouse model recapitulates key pathological features of AD such as a progressive accumulation of parenchymal amyloid plaques and vascular amyloid deposits, altered astroglial and microglial responses and elevation of CSF markers of neurodegeneration. Those observations were associated with increased TSPO and FDG-PET brain signals and a hyperactivity phenotype as the animals aged.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Discussion<\/jats:title>\n                    <jats:p>Our findings demonstrate that fibrillar A\u03b2 in microglia is associated with lipid dyshomeostasis consistent with lysosomal dysfunction and foam cell phenotypes as well as profound immuno-metabolic perturbations, opening new avenues to further investigate metabolic pathways at play in microglia responding to AD-relevant pathogenesis. The in-depth characterization of pathological hallmarks of AD in this novel and open-access mouse model should serve as a resource for the scientific community to investigate disease-relevant biology.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1186\/s13024-022-00547-7","type":"journal-article","created":{"date-parts":[[2022,6,11]],"date-time":"2022-06-11T07:03:04Z","timestamp":1654930984000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":123,"title":["Novel App knock-in mouse model shows key features of amyloid pathology and reveals profound metabolic dysregulation of 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