{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,7]],"date-time":"2026-07-07T05:34:33Z","timestamp":1783402473469,"version":"3.54.6"},"reference-count":41,"publisher":"Springer Science and Business Media LLC","issue":"1","license":[{"start":{"date-parts":[[2025,10,15]],"date-time":"2025-10-15T00:00:00Z","timestamp":1760486400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0"},{"start":{"date-parts":[[2025,10,15]],"date-time":"2025-10-15T00:00:00Z","timestamp":1760486400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0"}],"funder":[{"DOI":"10.13039\/501100003196","name":"Italian Ministry of Health","doi-asserted-by":"crossref","award":["Ricerca corrente"],"award-info":[{"award-number":["Ricerca corrente"]}],"id":[{"id":"10.13039\/501100003196","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100003196","name":"Italian Ministry of Health","doi-asserted-by":"crossref","award":["Ricerca corrente"],"award-info":[{"award-number":["Ricerca corrente"]}],"id":[{"id":"10.13039\/501100003196","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100003196","name":"Italian Ministry of Health","doi-asserted-by":"crossref","award":["Ricerca corrente"],"award-info":[{"award-number":["Ricerca corrente"]}],"id":[{"id":"10.13039\/501100003196","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100003196","name":"Italian Ministry of Health","doi-asserted-by":"crossref","award":["Ricerca corrente"],"award-info":[{"award-number":["Ricerca corrente"]}],"id":[{"id":"10.13039\/501100003196","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100003196","name":"Italian Ministry of Health","doi-asserted-by":"crossref","award":["Ricerca corrente"],"award-info":[{"award-number":["Ricerca corrente"]}],"id":[{"id":"10.13039\/501100003196","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100003196","name":"Italian Ministry of Health","doi-asserted-by":"crossref","award":["Ricerca corrente"],"award-info":[{"award-number":["Ricerca corrente"]}],"id":[{"id":"10.13039\/501100003196","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100003196","name":"Italian Ministry of Health","doi-asserted-by":"crossref","award":["Ricerca corrente"],"award-info":[{"award-number":["Ricerca corrente"]}],"id":[{"id":"10.13039\/501100003196","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100003196","name":"Italian Ministry of Health","doi-asserted-by":"crossref","award":["Ricerca corrente"],"award-info":[{"award-number":["Ricerca corrente"]}],"id":[{"id":"10.13039\/501100003196","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100003196","name":"Italian Ministry of Health","doi-asserted-by":"crossref","award":["Ricerca corrente"],"award-info":[{"award-number":["Ricerca corrente"]}],"id":[{"id":"10.13039\/501100003196","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Infect Agents Cancer"],"DOI":"10.1186\/s13027-025-00697-5","type":"journal-article","created":{"date-parts":[[2025,10,15]],"date-time":"2025-10-15T09:21:02Z","timestamp":1760520062000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["FAM19A4 and miR124-2 methylation status in human papillomavirus-driven and human papillomavirus-negative oropharyngeal squamous cell carcinomas"],"prefix":"10.1186","volume":"20","author":[{"given":"Maria Gabriella","family":"Don\u00e0","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eugenia","family":"Giuliani","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Valentina","family":"Laquintana","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Renato","family":"Covello","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Raul","family":"Pellini","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Silvia","family":"Moretto","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alessandra","family":"Latini","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria","family":"Benevolo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Francesca","family":"Rollo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2025,10,15]]},"reference":[{"issue":"1","key":"697_CR1","doi-asserted-by":"publisher","first-page":"62","DOI":"10.1007\/s00784-023-05425-0","volume":"28","author":"TC Fons\u00eaca","year":"2023","unstructured":"Fons\u00eaca TC, Jural LA, Mara\u00f1\u00f3n-V\u00e1squez GA, et al. Global prevalence of human papillomavirus-related oral and oropharyngeal squamous cell carcinomas: a systematic review and meta-analysis. Clin Oral Investig. 2023;28(1):62. https:\/\/doi.org\/10.1007\/s00784-023-05425-0.","journal-title":"Clin Oral Investig"},{"issue":"12","key":"697_CR2","doi-asserted-by":"publisher","first-page":"1319","DOI":"10.1016\/S1470-2045(14)70471-1","volume":"15","author":"C Ndiaye","year":"2014","unstructured":"Ndiaye C, Mena M, Alemany L, et al. HPV DNA, E6\/E7 mRNA, and p16INK4a detection in head and neck cancers: a systematic review and meta-analysis. Lancet Oncol. 2014;15(12):1319\u201331. https:\/\/doi.org\/10.1016\/S1470-2045(14)70471-1.","journal-title":"Lancet Oncol"},{"issue":"5","key":"697_CR3","doi-asserted-by":"publisher","first-page":"830","DOI":"10.1002\/ijc.34763","volume":"154","author":"Y Lu","year":"2024","unstructured":"Lu Y, Clifford GM, Fairley CK, et al. Human papillomavirus and p16INK4a in oropharyngeal squamous cell carcinomas: A systematic review and meta-analysis. Int J Cancer. 2024;154(5):830\u201341. https:\/\/doi.org\/10.1002\/ijc.34763.","journal-title":"Int J Cancer"},{"issue":"12","key":"697_CR4","doi-asserted-by":"publisher","first-page":"1544","DOI":"10.1093\/jnci\/djad169","volume":"115","author":"ZS Zumsteg","year":"2023","unstructured":"Zumsteg ZS, Luu M, Rosenberg PS, et al. Global epidemiologic patterns of oropharyngeal cancer incidence trends. J Natl Cancer Inst. 2023;115(12):1544\u201354. https:\/\/doi.org\/10.1093\/jnci\/djad169.","journal-title":"J Natl Cancer Inst"},{"key":"697_CR5","doi-asserted-by":"publisher","first-page":"24","DOI":"10.1056\/NEJMoa0912217","volume":"363","author":"KK Ang","year":"2010","unstructured":"Ang KK, Harris J, Wheeler R, et al. Human papillomavirus and survival of patients with oropharyngeal cancer. N Engl J Med. 2010;363:24\u201335. https:\/\/doi.org\/10.1056\/NEJMoa0912217.","journal-title":"N Engl J Med"},{"key":"697_CR6","doi-asserted-by":"publisher","first-page":"40","DOI":"10.1016\/j.radonc.2022.01.021","volume":"168","author":"P Gorphe","year":"2022","unstructured":"Gorphe P, Classe M, Ammari S, et al. Patterns of disease events and causes of death in patients with HPV-positive versus HPV-negative oropharyngeal carcinoma. Radiother Oncol. 2022;168:40\u20135. https:\/\/doi.org\/10.1016\/j.radonc.2022.01.021.","journal-title":"Radiother Oncol"},{"issue":"11","key":"697_CR7","doi-asserted-by":"publisher","first-page":"1986","DOI":"10.3390\/cancers16111986","volume":"16","author":"N Muresu","year":"2024","unstructured":"Muresu N, Puci MV, Sotgiu G, et al. Diagnostic accuracy of DNA-Methylation in detection of cervical dysplasia: findings from a Population-Based screening program. Cancers (Basel). 2024;16(11):1986. https:\/\/doi.org\/10.3390\/cancers16111986.","journal-title":"Cancers (Basel)"},{"issue":"26","key":"697_CR8","doi-asserted-by":"publisher","first-page":"3037","DOI":"10.1200\/JCO.21.02433","volume":"40","author":"WW Kremer","year":"2022","unstructured":"Kremer WW, Dick S, Heideman DAM, et al. Clinical regression of High-Grade cervical intraepithelial neoplasia is associated with absence of FAM19A4\/miR124-2 DNA methylation (CONCERVE Study). J Clin Oncol. 2022;40(26):3037\u201346. https:\/\/doi.org\/10.1200\/JCO.21.02433.","journal-title":"J Clin Oncol"},{"issue":"19","key":"697_CR9","doi-asserted-by":"publisher","first-page":"1951","DOI":"10.3390\/healthcare12191951","volume":"12","author":"N Muresu","year":"2024","unstructured":"Muresu N, Puci MV, Sotgiu G, et al. Diagnostic performance of host and viral DNA methylation analysis in the identification of anal intraepithelial neoplasia and cancer: systematic review and Meta-Analysis. Healthc (Basel). 2024;12(19):1951. https:\/\/doi.org\/10.3390\/healthcare12191951.","journal-title":"Healthc (Basel)"},{"issue":"9","key":"697_CR10","doi-asserted-by":"publisher","first-page":"2460","DOI":"10.1002\/ijc.32890","volume":"146","author":"T Nakagawa","year":"2020","unstructured":"Nakagawa T, Matsusaka K, Misawa K, et al. Stratification of HPV-associated and HPV-negative oropharyngeal squamous cell carcinomas based on DNA methylation epigenotypes. Int J Cancer. 2020;146(9):2460\u201374. https:\/\/doi.org\/10.1002\/ijc.32890.","journal-title":"Int J Cancer"},{"issue":"1","key":"697_CR11","doi-asserted-by":"publisher","first-page":"73","DOI":"10.4161\/15592294.2014.990787","volume":"10","author":"DM Schutze","year":"2015","unstructured":"Schutze DM, Kooter JM, Wilting SM, et al. Longitudinal assessment of DNA methylation changes during HPVE6E7-induced immortalization of primary keratinocytes. Epigenetics. 2015;10(1):73\u201381. https:\/\/doi.org\/10.4161\/15592294.2014.990787.","journal-title":"Epigenetics"},{"issue":"1","key":"697_CR12","doi-asserted-by":"publisher","first-page":"53","DOI":"10.1002\/path.4210","volume":"231","author":"RD Steenbergen","year":"2013","unstructured":"Steenbergen RD, Ongenaert M, Snellenberg S, et al. Methylation-specific digital karyotyping of HPV16E6E7-expressing human keratinocytes identifies novel methylation events in cervical carcinogenesis. J Pathol. 2013;231(1):53\u201362. https:\/\/doi.org\/10.1002\/path.4210.","journal-title":"J Pathol"},{"issue":"4","key":"697_CR13","doi-asserted-by":"publisher","first-page":"1424","DOI":"10.1158\/0008-5472.CAN-06-4218","volume":"67","author":"A Lujambio","year":"2007","unstructured":"Lujambio A, Ropero S, Ballestar E, et al. Genetic unmasking of an epigenetically silenced MicroRNA in human cancer cells. Cancer Res. 2007;67(4):1424\u20139. https:\/\/doi.org\/10.1158\/0008-5472.CAN-06-4218.","journal-title":"Cancer Res"},{"key":"697_CR14","doi-asserted-by":"publisher","first-page":"167","DOI":"10.1186\/1476-4598-9-167","volume":"9","author":"SM Wilting","year":"2010","unstructured":"Wilting SM, van Boerdonk RA, Henken FE, et al. Methylation-mediated Silencing and tumour suppressive function of hsa-miR-124 in cervical cancer. Mol Cancer. 2010;9:167. https:\/\/doi.org\/10.1186\/1476-4598-9-167.","journal-title":"Mol Cancer"},{"issue":"4","key":"697_CR15","doi-asserted-by":"publisher","first-page":"1215","DOI":"10.1002\/ijc.32614","volume":"147","author":"FJ Vink","year":"2020","unstructured":"Vink FJ, Meijer CJLM, Clifford GM, et al. FAM19A4\/miR124-2 methylation in invasive cervical cancer: A retrospective cross-sectional worldwide study. Int J Cancer. 2020;147(4):1215\u201321. https:\/\/doi.org\/10.1002\/ijc.32614.","journal-title":"Int J Cancer"},{"issue":"3","key":"697_CR16","doi-asserted-by":"publisher","first-page":"504","DOI":"10.1111\/1471-0528.16395","volume":"128","author":"WW Kremer","year":"2021","unstructured":"Kremer WW, Steenbergen R, Heideman D, Kenter GG, Meijer C. The use of host cell DNA methylation analysis in the detection and management of women with advanced cervical intraepithelial neoplasia: a review. BJOG. 2021;128(3):504\u201314. https:\/\/doi.org\/10.1111\/1471-0528.16395.","journal-title":"BJOG"},{"issue":"9","key":"697_CR17","doi-asserted-by":"publisher","first-page":"2262","DOI":"10.3390\/ijms20092262","volume":"20","author":"M Del Pino","year":"2019","unstructured":"Del Pino M, Sierra A, Marimon L, Mart\u00ed Delgado C, Rodriguez-Trujillo A, et al. CADM1, MAL, and miR124 promoter methylation as biomarkers of transforming cervical intrapithelial lesions. Int J Mol Sci. 2019;20(9):2262. https:\/\/doi.org\/10.3390\/ijms20092262.","journal-title":"Int J Mol Sci"},{"key":"697_CR18","doi-asserted-by":"publisher","first-page":"831949","DOI":"10.3389\/fonc.2022.831949","volume":"12","author":"L Zhang","year":"2022","unstructured":"Zhang L, Tan W, Yang H, Zhang S, Dai Y. Detection of host cell gene\/hpv DNA methylation markers: A promising triage approach for cervical cancer. Front Oncol. 2022;12:831949. https:\/\/doi.org\/10.3389\/fonc.2022.831949.","journal-title":"Front Oncol"},{"issue":"2","key":"697_CR19","doi-asserted-by":"publisher","first-page":"368","DOI":"10.1016\/j.ygyno.2019.06.002","volume":"154","author":"S Dick","year":"2019","unstructured":"Dick S, Kremer WW, De Strooper LMA, et al. Long-term CIN3\u2009+\u2009risk of HPV positive women after triage with FAM19A4\/miR124-2 methylation analysis. Gynecol Oncol. 2019;154(2):368\u201373. https:\/\/doi.org\/10.1016\/j.ygyno.2019.06.002.","journal-title":"Gynecol Oncol"},{"issue":"2","key":"697_CR20","doi-asserted-by":"publisher","first-page":"396","DOI":"10.1002\/ijc.33320","volume":"148","author":"J Bonde","year":"2021","unstructured":"Bonde J, Floore A, Ejegod D, et al. Methylation markers FAM19A4 and miR124-2 as triage strategy for primary human papillomavirus screen positive women: A large European multicenter study. Int J Cancer. 2021;148(2):396\u2013405. https:\/\/doi.org\/10.1002\/ijc.33320.","journal-title":"Int J Cancer"},{"issue":"1","key":"697_CR21","doi-asserted-by":"publisher","first-page":"125","DOI":"10.1186\/s13148-023-01537-2","volume":"15","author":"S Salta","year":"2023","unstructured":"Salta S, Lobo J, Magalh\u00e3es B, Henrique R, Jer\u00f3nimo C. DNA methylation as a triage marker for colposcopy referral in HPV-based cervical cancer screening: a systematic review and meta-analysis. Clin Epigenetics. 2023;15(1):125. https:\/\/doi.org\/10.1186\/s13148-023-01537-2.","journal-title":"Clin Epigenetics"},{"issue":"4","key":"697_CR22","doi-asserted-by":"publisher","first-page":"312","DOI":"10.3390\/pathogens13040312","volume":"13","author":"C Peronace","year":"2024","unstructured":"Peronace C, Cione E, Abrego-Guandique DM, et al. FAM19A4 and hsa-miR124-2 double methylation as screening for ASC-H- and CIN1 HPV-Positive women. Pathogens. 2024;13(4):312. https:\/\/doi.org\/10.3390\/pathogens13040312.","journal-title":"Pathogens"},{"issue":"2","key":"697_CR23","doi-asserted-by":"publisher","first-page":"378","DOI":"10.1111\/aogs.14707","volume":"103","author":"Y Lindroth","year":"2024","unstructured":"Lindroth Y, Pedersen L, Alssamaray J, et al. Cervix cytology samples revealed increased methylation of the human markers FAM19A4\/miR124-2 up to 8 years before adenocarcinoma. Acta Obstet Gynecol Scand. 2024;103(2):378\u201386. https:\/\/doi.org\/10.1111\/aogs.14707.","journal-title":"Acta Obstet Gynecol Scand"},{"issue":"10","key":"697_CR24","doi-asserted-by":"publisher","first-page":"1525","DOI":"10.1002\/cam4.499","volume":"4","author":"PM van Kempen","year":"2015","unstructured":"van Kempen PM, Noorlag R, Braunius WW, et al. Clinical relevance of copy number profiling in oral and oropharyngeal squamous cell carcinoma. Cancer Med. 2015;4(10):1525\u201335. https:\/\/doi.org\/10.1002\/cam4.499.","journal-title":"Cancer Med"},{"issue":"1","key":"697_CR25","doi-asserted-by":"publisher","first-page":"1146","DOI":"10.1186\/s12885-022-10227-w","volume":"22","author":"Y Inokawa","year":"2022","unstructured":"Inokawa Y, Hayashi M, Begum S, et al. High-risk HPV infection-associated hypermethylated genes in oropharyngeal squamous cell carcinomas. BMC Cancer. 2022;22(1):1146. https:\/\/doi.org\/10.1186\/s12885-022-10227-w.","journal-title":"BMC Cancer"},{"key":"697_CR26","doi-asserted-by":"publisher","first-page":"106568","DOI":"10.1016\/j.oraloncology.2023.106568","volume":"146","author":"KR Bartemes","year":"2023","unstructured":"Bartemes KR, Gochanour BR, Routman DM, et al. Assessing the capacity of methylated DNA markers of cervical squamous cell carcinoma to discriminate oropharyngeal squamous cell carcinoma in human papillomavirus mediated disease. Oral Oncol. 2023;146:106568. https:\/\/doi.org\/10.1016\/j.oraloncology.2023.106568.","journal-title":"Oral Oncol"},{"issue":"11","key":"697_CR27","doi-asserted-by":"publisher","first-page":"1301","DOI":"10.1080\/15592294.2021.2018812","volume":"17","author":"N Birknerov\u00e1","year":"2022","unstructured":"Birknerov\u00e1 N, Kova\u0159\u00edkov\u00e1 H, Baranov\u00e1 I, et al. DNA hypermethylation of CADM1, PAX5, WT1, RAR\u03b2, and PAX6 genes in oropharyngeal cancer associated with human papillomavirus. Epigenetics. 2022;17(11):1301\u201310. https:\/\/doi.org\/10.1080\/15592294.2021.2018812.","journal-title":"Epigenetics"},{"issue":"2","key":"697_CR28","doi-asserted-by":"publisher","first-page":"254","DOI":"10.1002\/hed.24575","volume":"39","author":"MG Don\u00e0","year":"2017","unstructured":"Don\u00e0 MG, Pichi B, Rollo F, et al. Mucosal and cutaneous human papillomaviruses in head and neck squamous cell papillomas. Head Neck. 2017;39(2):254\u20139. https:\/\/doi.org\/10.1002\/hed.24575.","journal-title":"Head Neck"},{"issue":"9","key":"697_CR29","doi-asserted-by":"publisher","first-page":"e0184623","DOI":"10.1371\/journal.pone.0184623","volume":"12","author":"F Rollo","year":"2017","unstructured":"Rollo F, Latini A, Pichi B, et al. Prevalence and determinants of oral infection by human papillomavirus in HIV-infected and uninfected men who have sex with men. PLoS ONE. 2017;12(9):e0184623. https:\/\/doi.org\/10.1371\/journal.pone.0184623.","journal-title":"PLoS ONE"},{"issue":"8","key":"697_CR30","doi-asserted-by":"publisher","first-page":"1283","DOI":"10.2217\/FMB.15.55","volume":"10","author":"MG Don\u00e0","year":"2015","unstructured":"Don\u00e0 MG, Spriano G, Pichi B, et al. Human papillomavirus infection and p16 overexpression in oropharyngeal squamous cell carcinoma: a case series from 2010 to 2014. Future Microbiol. 2015;10(8):1283\u201391. https:\/\/doi.org\/10.2217\/FMB.15.55.","journal-title":"Future Microbiol"},{"issue":"7","key":"697_CR31","doi-asserted-by":"publisher","first-page":"528","DOI":"10.1136\/sextrans-2019-054301","volume":"96","author":"M Giuliani","year":"2020","unstructured":"Giuliani M, Rollo F, Vescio MF, et al. Oral human papillomavirus infection in HIV-infected and HIV-uninfected MSM: the OHMAR prospective cohort study. Sex Transm Infect. 2020;96(7):528\u201336. https:\/\/doi.org\/10.1136\/sextrans-2019-054301.","journal-title":"Sex Transm Infect"},{"issue":"Pt B","key":"697_CR32","first-page":"1","volume":"100","author":"IARC Working Group on the Evaluation of Carcinogenic Risks to Humans","year":"2012","unstructured":"IARC Working Group on the Evaluation of Carcinogenic Risks to Humans. Biological agents. IARC Monogr Eval Carcinog Risks Hum. 2012;100(Pt B):1\u2013441.","journal-title":"IARC Monogr Eval Carcinog Risks Hum"},{"key":"697_CR33","first-page":"113","volume-title":"American joint committee on cancer: cancer staging manual","author":"B O\u2019Sullivan","year":"2017","unstructured":"O\u2019Sullivan B, Lydiatt WM, Haughey BH. HPV-mediated (p16+) oropharyngeal cancer. In: Amin MB, Edge S, Greene FL, editors. American joint committee on cancer: cancer staging manual. New York: Springer; 2017. pp. 113\u201351."},{"issue":"6","key":"697_CR34","doi-asserted-by":"publisher","first-page":"1101","DOI":"10.1038\/nprot.2008.73","volume":"3","author":"TD Schmittgen","year":"2008","unstructured":"Schmittgen TD, Livak KJ. Analyzing real-time PCR data by the comparative C(T) method. Nat Protoc. 2008;3(6):1101\u20138. https:\/\/doi.org\/10.1038\/nprot.2008.73.","journal-title":"Nat Protoc"},{"key":"697_CR35","doi-asserted-by":"publisher","first-page":"856","DOI":"10.1093\/nar\/gku1151","volume":"43","author":"W Huang","year":"2015","unstructured":"Huang W, Hsu S, Huang H, et al. MethHC: a database of DNA methylation and gene expression in human cancer. Nucleic Acids Res. 2015;43:856. https:\/\/doi.org\/10.1093\/nar\/gku1151.","journal-title":"Nucleic Acids Res"},{"key":"697_CR36","doi-asserted-by":"publisher","first-page":"D1268","DOI":"10.1093\/nar\/gkaa1104","volume":"49","author":"H Huang","year":"2021","unstructured":"Huang H, Li J, Tang Y, et al. MethHC 2.0: information repository of DNA methylation and gene expression in human cancer. Nucleic Acids Res. 2021;49:D1268\u201375. https:\/\/doi.org\/10.1093\/nar\/gkaa1104.","journal-title":"Nucleic Acids Res"},{"key":"697_CR37","doi-asserted-by":"publisher","first-page":"597788","DOI":"10.3389\/fonc.2020.597788","volume":"10","author":"CM Lopes-Ramos","year":"2020","unstructured":"Lopes-Ramos CM, Quackenbush J, DeMeo DL. Genome-Wide sex and gender differences in cancer. Front Oncol. 2020;10:597788. https:\/\/doi.org\/10.3389\/fonc.2020.597788.","journal-title":"Front Oncol"},{"key":"697_CR38","doi-asserted-by":"publisher","first-page":"436","DOI":"10.1161\/CIRCGENETICS.116.001506","volume":"9","author":"R Joehanes","year":"2016","unstructured":"Joehanes R, Just AC, Marioni RE, et al. Epigenetic signatures of cigarette smoking. Circ Cardiovasc Genet. 2016;9:436\u201347. https:\/\/doi.org\/10.1161\/CIRCGENETICS.116.001506.","journal-title":"Circ Cardiovasc Genet"},{"key":"697_CR39","doi-asserted-by":"publisher","first-page":"152","DOI":"10.1097\/CCO.0000000000000444","volume":"30","author":"Y Ghantous","year":"2018","unstructured":"Ghantous Y, Schussel JL, Brait M. Tobacco and alcohol-induced epigenetic changes in oral carcinoma. Curr Opin Oncol. 2018;30:152\u20138. https:\/\/doi.org\/10.1097\/CCO.0000000000000444.","journal-title":"Curr Opin Oncol"},{"key":"697_CR40","doi-asserted-by":"publisher","first-page":"105947","DOI":"10.1016\/j.oraloncology.2022.105947","volume":"131","author":"MG Don\u00e0","year":"2022","unstructured":"Don\u00e0 MG, Rollo F, Pichi B, et al. Updates on human Papillomavirus-driven oropharyngeal squamous cell carcinomas in a Southern European country. Oral Oncol. 2022;131:105947. https:\/\/doi.org\/10.1016\/j.oraloncology.2022.105947.","journal-title":"Oral Oncol"},{"key":"697_CR41","unstructured":"Ferlay J, Ervik M, Lam F et al. Global cancer observatory: cancer today. 2024. Lyon, France: International Agency for Research on Cancer. https:\/\/gco.iarc.who.int\/today. Accessed 6 March 2025."}],"container-title":["Infectious Agents and Cancer"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1186\/s13027-025-00697-5.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/article\/10.1186\/s13027-025-00697-5\/fulltext.html","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1186\/s13027-025-00697-5.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,15]],"date-time":"2025-10-15T09:21:06Z","timestamp":1760520066000},"score":1,"resource":{"primary":{"URL":"https:\/\/infectagentscancer.biomedcentral.com\/articles\/10.1186\/s13027-025-00697-5"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,10,15]]},"references-count":41,"journal-issue":{"issue":"1","published-online":{"date-parts":[[2025,12]]}},"alternative-id":["697"],"URL":"https:\/\/doi.org\/10.1186\/s13027-025-00697-5","relation":{},"ISSN":["1750-9378"],"issn-type":[{"value":"1750-9378","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,10,15]]},"assertion":[{"value":"30 May 2025","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"29 August 2025","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"15 October 2025","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Declarations"}},{"value":"The study was conducted according to the guidelines of the Declaration of Helsinki and approved by the Comitato Etico Territoriale Lazio Area 5 (IFO\/51\/23, 25 Oct 2023). The Ethics Committee also granted exemption from informed consent.","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Ethics approval and consent to participate"}},{"value":"Francesca Rollo has received financial and non-financial support from Fujirebio Europe N.V. that provided the kits used for methylation analysis at no cost. Fujirebio Europe N.V. had no role in the design of the study, in the collection, analyses, or interpretation of data, in the writing of the manuscript, or in the decision to publish the results. Maria Benevolo has received non-financial support from Roche Diagnostics and Hologic S.r.L. and financial and non-financial support from Becton and Dickinson for work performed outside the range of the current study. The other authors declare no conflicts of interest.","order":3,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing interests"}}],"article-number":"71"}}