{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,7]],"date-time":"2025-11-07T09:34:27Z","timestamp":1762508067301},"reference-count":24,"publisher":"Springer Science and Business Media LLC","issue":"1","license":[{"start":{"date-parts":[[2020,4,25]],"date-time":"2020-04-25T00:00:00Z","timestamp":1587772800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2020,4,25]],"date-time":"2020-04-25T00:00:00Z","timestamp":1587772800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"AniCura Research Fund"}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Acta Vet Scand"],"published-print":{"date-parts":[[2020,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Sweden has a long tradition of monitoring occurrence of antibiotic resistant bacteria in both animals and humans, but there currently is no organised and harmonized monitoring on carriage of Enterobacteriaceae producing extended-spectrum beta-lactamase (ESBL), plasmid-mediated AmpC beta-lactamase (pAmpC), or methicillin-resistant coagulase positive staphylococci e.g. methicillin-resistant <jats:italic>Staphylococcus aureus<\/jats:italic> (MRSA) and methicillin-resistant <jats:italic>Staphylococcus pseudintermedius<\/jats:italic> (MRSP) in dogs. The aim of the current study was therefore to determine the prevalence of ESBL\/pAmpC producing Enterobacteriaceae and methicillin-resistant coagulase positive staphylococci in healthy dogs in Sweden, and to phenotypically and genotypically characterize any identified isolates. It was shown that 0.9% (95% confident interval 0.3\u20132.7%) of the dogs (n\u2009=\u2009325) carried multi-resistant ESBL-producing <jats:italic>Escherichia coli<\/jats:italic>, but that no methicillin-resistant coagulase positive staphylococci could be detected. In conclusion, the occurrence of multi-drug resistant bacteria remains rare among healthy dogs in Sweden. In addition, the ESBL-producing <jats:italic>E. coli<\/jats:italic> identified showed genetic characteristics related to those reported from humans.<\/jats:p>","DOI":"10.1186\/s13028-020-00516-4","type":"journal-article","created":{"date-parts":[[2020,4,25]],"date-time":"2020-04-25T20:02:26Z","timestamp":1587844946000},"update-policy":"http:\/\/dx.doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Low occurrence of extended-spectrum cephalosporinase producing Enterobacteriaceae and no detection of methicillin-resistant coagulase-positive staphylococci in healthy dogs in Sweden"],"prefix":"10.1186","volume":"62","author":[{"given":"Stefan","family":"B\u00f6rjesson","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lotta","family":"Gunnarsson","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Annica","family":"Land\u00e9n","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ulrika","family":"Gr\u00f6nlund","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2020,4,25]]},"reference":[{"key":"516_CR1","doi-asserted-by":"publisher","first-page":"571","DOI":"10.2147\/IDR.S189884","volume":"12","author":"G Abbas","year":"2019","unstructured":"Abbas G, Khan I, Mohsin M, Sajjad Ur R, Younas T, Ali S. High rates of CTX-M group-1 extended-spectrum beta-lactamases producing Escherichia coli from pets and their owners in Faisalabad, Pakistan. Infect Drug Resist. 2019;12:571\u20138.","journal-title":"Infect Drug Resist."},{"key":"516_CR2","doi-asserted-by":"publisher","first-page":"83","DOI":"10.1186\/s13028-015-0174-3","volume":"57","author":"P Damborg","year":"2015","unstructured":"Damborg P, Morsing MK, Petersen T, Bortolaia V, Guardabassi L. CTX-M-1 and CTX-M-15-producing Escherichia coli in dog faeces from public gardens. Acta Vet Scand. 2015;57:83.","journal-title":"Acta Vet Scand"},{"key":"516_CR3","doi-asserted-by":"publisher","first-page":"5358","DOI":"10.1128\/AAC.02545-14","volume":"58","author":"M Haenni","year":"2014","unstructured":"Haenni M, Saras E, Metayer V, Medaille C, Madec JY. High prevalence of blaCTX-M-1\/IncI1\/ST3 and blaCMY-2\/IncI1\/ST2 plasmids in healthy urban dogs in France. Antimicrob Agents Chemother. 2014;58:5358\u201362.","journal-title":"Antimicrob Agents Chemother"},{"key":"516_CR4","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dkz462","author":"G van den Bunt","year":"2019","unstructured":"van den Bunt G, Fluit AC, Spaninks MP, Timmerman AJ, Geurts Y, Kant A, et al. Faecal carriage, risk factors, acquisition and persistence of ESBL-producing Enterobacteriaceae in dogs and cats and co-carriage with humans belonging to the same household. J Antimicrob Chemother. 2019. https:\/\/doi.org\/10.1093\/jac\/dkz462.","journal-title":"J Antimicrob Chemother"},{"key":"516_CR5","doi-asserted-by":"publisher","first-page":"23","DOI":"10.1016\/j.vetmic.2016.11.017","volume":"199","author":"AL Wedley","year":"2017","unstructured":"Wedley AL, Dawson S, Maddox TW, Coyne KP, Pinchbeck GL, Clegg P, et al. Carriage of antimicrobial resistant Escherichia coli in dogs: prevalence, associated risk factors and molecular characteristics. Vet Microbiol. 2017;199:23\u201330.","journal-title":"Vet Microbiol."},{"key":"516_CR6","doi-asserted-by":"publisher","first-page":"1024","DOI":"10.1292\/jvms.16-0432","volume":"79","author":"O Aslantas","year":"2017","unstructured":"Aslantas O, Yilmaz ES. Prevalence and molecular characterization of extended-spectrum beta-lactamase (ESBL) and plasmidic AmpC beta-lactamase (pAmpC) producing Escherichia coli in dogs. J Vet Med Sci. 2017;79:1024\u201330.","journal-title":"J Vet Med Sci"},{"key":"516_CR7","doi-asserted-by":"publisher","first-page":"602","DOI":"10.1111\/j.1472-765X.2006.02018.x","volume":"43","author":"M Vengust","year":"2006","unstructured":"Vengust M, Anderson ME, Rousseau J, Weese JS. Methicillin-resistant staphylococcal colonization in clinically normal dogs and horses in the community. Lett Appl Microbiol. 2006;43:602\u20136.","journal-title":"Lett Appl Microbiol"},{"key":"516_CR8","first-page":"1047","volume":"50","author":"C Murphy","year":"2009","unstructured":"Murphy C, Reid-Smith RJ, Prescott JF, Bonnett BN, Poppe C, Boerlin P, et al. Occurrence of antimicrobial resistant bacteria in healthy dogs and cats presented to private veterinary hospitals in southern Ontario: a preliminary study. Can Vet J. 2009;50:1047\u201353.","journal-title":"Can Vet J"},{"key":"516_CR9","doi-asserted-by":"publisher","first-page":"123","DOI":"10.1111\/vop.12130","volume":"18","author":"MC Mouney","year":"2015","unstructured":"Mouney MC, Stiles J, Townsend WM, Guptill L, Weese JS. Prevalence of methicillin-resistant Staphylococcus spp. in the conjunctival sac of healthy dogs. Vet Ophthalmol. 2015;18:123\u20136.","journal-title":"Vet Ophthalmol."},{"key":"516_CR10","doi-asserted-by":"publisher","first-page":"37","DOI":"10.1186\/s13028-015-0129-8","volume":"57","author":"T Gronthal","year":"2015","unstructured":"Gronthal T, Ollilainen M, Eklund M, Piiparinen H, Gindonis V, Junnila J, et al. Epidemiology of methicillin resistant Staphylococcus pseudintermedius in guide dogs in Finland. Acta Vet Scand. 2015;57:37.","journal-title":"Acta Vet Scand"},{"key":"516_CR11","unstructured":"Swedres-Svarm 2018. Consumption of antibiotics and occurnce of resistance in Sweden Solna\/Uppsala ISSN1650-6332."},{"key":"516_CR12","doi-asserted-by":"publisher","first-page":"395","DOI":"10.1111\/j.1469-0691.2011.03715.x","volume":"18","author":"M Stegger","year":"2012","unstructured":"Stegger M, Andersen PS, Kearns A, Pichon B, Holmes MA, Edwards G, et al. Rapid detection, differentiation and typing of methicillin-resistant Staphylococcus aureus harbouring either mecA or the new mecA homologue mecA(LGA251). Clin Microbiol Infect. 2012;18:395\u2013400.","journal-title":"Clin Microbiol Infect"},{"key":"516_CR13","doi-asserted-by":"publisher","unstructured":"Borjesson S, Greko C, Myrenas M, Landen A, Nilsson O, Pedersen K. A link between the newly described colistin resistance gene mcr-9 and clinical Enterobacteriaceae isolates carrying blaSHV-12 from horses in Sweden. J Glob Antimicrob Resist. https:\/\/doi.org\/10.1016\/j.jgar.2019.08.007. (In press).","DOI":"10.1016\/j.jgar.2019.08.007"},{"key":"516_CR14","doi-asserted-by":"publisher","unstructured":"van den Bunt G, van Pelt W, Hidalgo L, Scharringa J, de Greeff SC, Schurch AC, et al. Prevalence, risk factors and genetic characterisation of extended-spectrum beta-lactamase and carbapenemase-producing Enterobacteriaceae (ESBL-E and CPE): a community-based cross-sectional study, the Netherlands, 2014 to 2016. Euro Surveill. 2019:24(41). https:\/\/doi.org\/10.2807\/1560-7917.ES.2019.24.41.1800594","DOI":"10.2807\/1560-7917.ES.2019.24.41.1800594"},{"key":"516_CR15","doi-asserted-by":"publisher","first-page":"582","DOI":"10.1093\/jac\/dkw419","volume":"72","author":"S Ny","year":"2017","unstructured":"Ny S, Lofmark S, Borjesson S, Englund S, Ringman M, Bergstrom J, et al. Community carriage of ESBL-producing Escherichia coli is associated with strains of low pathogenicity: a Swedish nationwide study. J Antimicrob Chemother. 2017;72:582\u20138.","journal-title":"J Antimicrob Chemother"},{"key":"516_CR16","doi-asserted-by":"publisher","first-page":"158","DOI":"10.3389\/fvets.2019.00158","volume":"6","author":"NEM Hopman","year":"2019","unstructured":"Hopman NEM, van Dijk MAM, Broens EM, Wagenaar JA, Heederik DJJ, van Geijlswijk IM. Quantifying antimicrobial use in Dutch companion animals. Front Vet Sci. 2019;6:158.","journal-title":"Front Vet Sci."},{"key":"516_CR17","doi-asserted-by":"publisher","first-page":"136","DOI":"10.1016\/j.prevetmed.2018.12.002","volume":"162","author":"A Dame-Korevaar","year":"2019","unstructured":"Dame-Korevaar A, Fischer EAJ, van der Goot J, Stegeman A, Mevius D. Transmission routes of ESBL\/pAmpC producing bacteria in the broiler production pyramid, a literature review. Prev Vet Med. 2019;162:136\u201350.","journal-title":"Prev Vet Med."},{"key":"516_CR18","doi-asserted-by":"publisher","first-page":"2463","DOI":"10.1128\/AEM.03893-12","volume":"79","author":"S Borjesson","year":"2013","unstructured":"Borjesson S, Egervarn M, Lindblad M, Englund S. Frequent occurrence of extended-spectrum beta-lactamase- and transferable ampc beta-lactamase-producing Escherichia coli on domestic chicken meat in Sweden. Appl Environ Microbiol. 2013;79:2463\u20136.","journal-title":"Appl Environ Microbiol"},{"key":"516_CR19","doi-asserted-by":"publisher","first-page":"3","DOI":"10.1186\/1751-0147-55-3","volume":"55","author":"S Borjesson","year":"2013","unstructured":"Borjesson S, Bengtsson B, Jernberg C, Englund S. Spread of extended-spectrum beta-lactamase producing Escherichia coli isolates in Swedish broilers mediated by an incl plasmid carrying bla(CTX-M-1). Acta Vet Scand. 2013;55:3.","journal-title":"Acta Vet Scand"},{"key":"516_CR20","first-page":"28758","volume":"5","author":"O Nilsson","year":"2015","unstructured":"Nilsson O. Hygiene quality and presence of ESBL-producing Escherichia coli in raw food diets for dogs. Infect Ecol Epidemol. 2015;5:28758.","journal-title":"Infect Ecol Epidemol."},{"key":"516_CR21","doi-asserted-by":"publisher","first-page":"83","DOI":"10.1089\/mdr.2010.0132","volume":"17","author":"U Naseer","year":"2011","unstructured":"Naseer U, Sundsfjord A. The CTX-M conundrum: dissemination of plasmids and Escherichia coli clones. Micro Drug Resist. 2011;17:83\u201397.","journal-title":"Micro Drug Resist."},{"key":"516_CR22","first-page":"31514","volume":"6","author":"O Ljungquist","year":"2016","unstructured":"Ljungquist O, Ljungquist D, Myrenas M, Ryden C, Finn M, Bengtsson B. Evidence of household transfer of ESBL-\/pAmpC-producing Enterobacteriaceae between humans and dogs\u2014a pilot study. Infect Ecol Epidemol. 2016;6:31514.","journal-title":"Infect Ecol Epidemol."},{"issue":"12","key":"516_CR23","doi-asserted-by":"publisher","first-page":"e0190380","DOI":"10.1371\/journal.pone.0190380","volume":"12","author":"C Atterby","year":"2017","unstructured":"Atterby C, Borjesson S, Ny S, Jarhult JD, Byfors S, Bonnedahl J. ESBL-producing Escherichia coli in Swedish gulls\u2014a case of environmental pollution from humans? PLoS ONE. 2017;12(12):e0190380.","journal-title":"PLoS ONE"},{"issue":"1","key":"516_CR24","first-page":"1701399","volume":"9","author":"R S\u00f6derlund","year":"2019","unstructured":"S\u00f6derlund R, Skarin H, B\u00f6rjesson S, Sann\u00f6 A, Jernberg T, Aspan A, et al. Prevalence and genomic characteristics of zoonotic gastro-intestinal pathogens and ESBL\/pAmpC producing Enterobacteriaceae among Swedish corvid birds. Infect Ecol Epidemol. 2019;9(1):1701399.","journal-title":"Infect Ecol Epidemol."}],"container-title":["Acta Veterinaria Scandinavica"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1186\/s13028-020-00516-4.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/article\/10.1186\/s13028-020-00516-4\/fulltext.html","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1186\/s13028-020-00516-4.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,4,24]],"date-time":"2021-04-24T23:41:36Z","timestamp":1619307696000},"score":1,"resource":{"primary":{"URL":"https:\/\/actavetscand.biomedcentral.com\/articles\/10.1186\/s13028-020-00516-4"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,4,25]]},"references-count":24,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2020,12]]}},"alternative-id":["516"],"URL":"https:\/\/doi.org\/10.1186\/s13028-020-00516-4","relation":{},"ISSN":["1751-0147"],"issn-type":[{"value":"1751-0147","type":"electronic"}],"subject":[],"published":{"date-parts":[[2020,4,25]]},"assertion":[{"value":"7 January 2020","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"15 April 2020","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"25 April 2020","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"Ethical approval was not needed since the study was performed anonymously and the sampling was performed by trained professionals during routine visits at veterinary hospitals. Information and consent for sampling was given by the dog owners when visiting the veterinary hospitals. The study was approved by Animal Welfare Body at SVA on the 2017-03-06, SVA32314.","order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Ethics approval and consent to participate"}},{"value":"Not applicable.","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Consent for publication"}},{"value":"The authors declare that they have no competing interests.","order":3,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing interests"}}],"article-number":"18"}}