{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,24]],"date-time":"2026-10-24T00:40:46Z","timestamp":1792802446332,"version":"4.5.0"},"reference-count":49,"publisher":"Springer Science and Business Media LLC","issue":"1","license":[{"start":{"date-parts":[[2020,6,24]],"date-time":"2020-06-24T00:00:00Z","timestamp":1592956800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"},{"start":{"date-parts":[[2020,6,24]],"date-time":"2020-06-24T00:00:00Z","timestamp":1592956800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"}],"funder":[{"name":"Svenska Forskningsr\u00e5det Formas","award":["dnr 2016-01118"],"award-info":[{"award-number":["dnr 2016-01118"]}]}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Acta Vet Scand"],"published-print":{"date-parts":[[2020,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p><jats:italic>Streptococcus suis<\/jats:italic>is a major cause of meningitis, arthritis, and pneumonia in pigs worldwide, and an emerging pathogen in humans. In Sweden,<jats:italic>S. suis<\/jats:italic>has previously received little attention but has in recent years become increasingly recognized as affecting the pig production. The aim of the present study was to investigate the occurrence, serotypes and antimicrobial susceptibility of<jats:italic>S. suis<\/jats:italic>in Swedish grower pigs from herds with and without reported<jats:italic>S. suis<\/jats:italic>associated disease, as well as possible associations between<jats:italic>S. suis<\/jats:italic>associated disease and selected environmental and production factors. Swab samples were taken from the tonsils of clinically healthy 8\u201313-week-old grower pigs from ten case herds and ten control herds. Isolates were cultured, identified using MALDI\u2013TOF MS, and serotyped using latex agglutination. The antimicrobial susceptibility of 188 isolates was tested using broth microdilution. Production data was gathered and environmental parameters were measured on the farms.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p><jats:italic>Streptococcus suis<\/jats:italic>was isolated from 95% of the sampled pigs in both the case and the control herds. Serotypes 3, 4, 5, 7, 9, 10, 11, 15, 16, and 17\u201334 were detected, although a majority of the isolates (81.5%) were non-typeable. There was less diversity among the serotypes isolated from the case herds than among those from the control herds; four and nine different serotypes, respectively. Isolates resistant to penicillin (3.8%) were reported for the first time in Sweden. Tetracycline resistance was common (88.4%). No association was noted between the production and the environmental factors investigated, and the carriership of<jats:italic>S. suis<\/jats:italic>.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>The carriership of<jats:italic>S. suis<\/jats:italic>was found to be higher in clinically healthy Swedish pigs than previously estimated, and for the first time, the presence of Swedish isolates resistant to penicillin was reported. Many of the most commonly disease-associated serotypes, e.g. serotypes 2, 9, 3, and 7, were detected in healthy grower pigs although further studies are needed to investigate the virulence of these isolates.<\/jats:p><\/jats:sec>","DOI":"10.1186\/s13028-020-00533-3","type":"journal-article","created":{"date-parts":[[2020,6,24]],"date-time":"2020-06-24T12:03:08Z","timestamp":1593000188000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":45,"title":["Streptococcus suis in Swedish grower pigs: occurrence, serotypes, and antimicrobial susceptibility"],"prefix":"10.1186","volume":"62","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7076-5808","authenticated-orcid":false,"given":"Anna","family":"Werinder","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anna","family":"Asp\u00e1n","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Annette","family":"Backhans","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marie","family":"Sj\u00f6lund","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bengt","family":"Guss","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Magdalena","family":"Jacobson","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2020,6,24]]},"reference":[{"key":"533_CR1","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1016\/S0007-1935(17)30581-X","volume":"139","author":"FA Clifton-Hadley","year":"1983","unstructured":"Clifton-Hadley FA. Streptococcus suis type 2 infections. Br Vet J. 1983;139:1\u20135.","journal-title":"Br Vet J"},{"key":"533_CR2","doi-asserted-by":"publisher","first-page":"201","DOI":"10.1016\/S1473-3099(07)70001-4","volume":"7","author":"Z-R Lun","year":"2007","unstructured":"Lun Z-R, Wang Q-P, Chen X-G, Li A-X, Zhu X-Q. Streptococcus suis: an emerging zoonotic pathogen. Lancet Infect Dis. 2007;7:201\u20139.","journal-title":"Lancet Infect Dis."},{"key":"533_CR3","doi-asserted-by":"publisher","first-page":"31","DOI":"10.1111\/j.1365-2672.1994.tb03040.x","volume":"77","author":"LA Devriese","year":"1994","unstructured":"Devriese LA, Hommez J, Pot B, Haesebrouck F. Identification and composition of the streptococcal and enterococcal flora of tonsils, intestines and faeces of pigs. J Appl Bacteriol. 1994;77:31\u20136.","journal-title":"J Appl Bacteriol"},{"key":"533_CR4","doi-asserted-by":"publisher","first-page":"945","DOI":"10.1128\/JCM.20.5.945-947.1984","volume":"20","author":"JP Arends","year":"1984","unstructured":"Arends JP, Hartwig N, Rudolphy M, Zanen HC. Carrier rate of Streptococcus suis capsular type 2 in palatine tonsils of slaughtered pigs. J Clin Microbiol. 1984;20:945\u20137.","journal-title":"J Clin Microbiol"},{"key":"533_CR5","doi-asserted-by":"publisher","first-page":"e61339","DOI":"10.1371\/journal.pone.0061339","volume":"8","author":"N Dekker","year":"2013","unstructured":"Dekker N, Bouma A, Daemen I, Klinkenberg D, Leengoed LV, Wagenaar JA, et al. Effect of spatial separation of pigs on spread of Streptococcus suis serotype 9. PLOS ONE. 2013;8:e61339.","journal-title":"PLOS ONE."},{"key":"533_CR6","first-page":"269","volume":"4","author":"SF Amass","year":"1996","unstructured":"Amass SF, Clark LK, Knox K, Wu CC, Hill MA. Streptococcus suis colonization of piglets during parturition. Swine Health Prod. 1996;4:269\u201372.","journal-title":"Swine Health Prod."},{"key":"533_CR7","doi-asserted-by":"publisher","first-page":"e02590-17","DOI":"10.1128\/AEM.02590-17","volume":"84","author":"G Zou","year":"2018","unstructured":"Zou G, Zhou J, Xiao R, Zhang L, Cheng Y, Jin H, et al. Effects of environmental and management-associated factors on prevalence and diversity of Streptococcus suis in clinically healthy pig herds in China and the United Kingdom. Appl Environ Microb. 2018;84:e02590-17.","journal-title":"Appl Environ Microb."},{"key":"533_CR8","doi-asserted-by":"crossref","first-page":"295","DOI":"10.2460\/javma.1993.203.02.295","volume":"203","author":"SA Dee","year":"1993","unstructured":"Dee SA, Carlson AR, Winkelman NL, Corey MM. Effect of management practices on the Streptococcus suis carrier rate in nursery swine. J Am Vet Med Assoc. 1993;203:295\u20139.","journal-title":"J Am Vet Med Assoc"},{"key":"533_CR9","doi-asserted-by":"publisher","first-page":"60","DOI":"10.1136\/vr.134.3.60","volume":"134","author":"L Galina","year":"1994","unstructured":"Galina L, Pijoan C, Sitjar M, Christianson WT, Rossow K, Collins JE. Interaction between Streptococcus suis serotype 2 and porcine reproductive and respiratory syndrome virus in specific pathogen-free piglets. Vet Rec. 1994;134:60\u20134.","journal-title":"Vet Rec."},{"issue":"Pt 2","key":"533_CR10","doi-asserted-by":"publisher","first-page":"581","DOI":"10.1099\/00207713-48-2-581","volume":"48","author":"S Chatellier","year":"1998","unstructured":"Chatellier S, Harel J, Zhang Y, Gottschalk M, Higgins R, Devriese LA, et al. Phylogenetic diversity of Streptococcus suis strains of various serotypes as revealed by 16S rRNA gene sequence comparison. Int J Syst Bacteriol. 1998;48(Pt 2):581\u20139.","journal-title":"Int J Syst Bacteriol"},{"key":"533_CR11","doi-asserted-by":"publisher","first-page":"63","DOI":"10.1016\/j.vetmic.2005.01.003","volume":"107","author":"JE Hill","year":"2005","unstructured":"Hill JE, Gottschalk M, Brousseau R, Harel J, Hemmingsen SM, Goh SH. Biochemical analysis, cpn60 and 16S rDNA sequence data indicate that Streptococcus suis serotypes 32 and 34, isolated from pigs, are Streptococcus orisratti. Vet Microbiol. 2005;107:63\u20139.","journal-title":"Vet Microbiol"},{"key":"533_CR12","doi-asserted-by":"publisher","first-page":"842","DOI":"10.1016\/j.vetmic.2012.11.001","volume":"162","author":"LHT Tien","year":"2013","unstructured":"Tien LHT, Nishibori T, Nishitani Y, Nomoto R, Osawa R. Reappraisal of the taxonomy of Streptococcus suis serotypes 20, 22, 26, and 33 based on DNA-DNA homology and sodA and recN phylogenies. Vet Microbiol. 2013;162:842\u20139.","journal-title":"Vet Microbiol"},{"key":"533_CR13","doi-asserted-by":"publisher","first-page":"e45","DOI":"10.1038\/emi.2014.45","volume":"3","author":"G Goyette-Desjardins","year":"2014","unstructured":"Goyette-Desjardins G, Auger JP, Xu J, Segura M, Gottschalk M. Streptococcus suis, an important pig pathogen and emerging zoonotic agent-an update on the worldwide distribution based on serotyping and sequence typing. Emerg Microbes Infect. 2014;3:e45.","journal-title":"Emerg Microbes Infect."},{"key":"533_CR14","first-page":"170","volume":"34","author":"JLM Flores","year":"1993","unstructured":"Flores JLM, Higgins R, D\u2019Allaire S, Charette R, Boudreau M, Gottschalk M. Distribution of the different capsular types of Streptococcus suis in nineteen swine nurseries. Can Vet J. 1993;34:170.","journal-title":"Can Vet J"},{"key":"533_CR15","doi-asserted-by":"publisher","first-page":"119","DOI":"10.1177\/104063879600800121","volume":"8","author":"RY Reams","year":"1996","unstructured":"Reams RY, Harrington DD, Glickman LT, Thacker HL, Bowersock TL. Multiple serotypes and strains of Streptococcus suis in naturally infected swine herds. J Vet Diagn Investig. 1996;8:119\u201321.","journal-title":"J Vet Diagn Investig"},{"key":"533_CR16","doi-asserted-by":"publisher","first-page":"68","DOI":"10.1017\/S1466252313000029","volume":"14","author":"NP Varela","year":"2013","unstructured":"Varela NP, Gadbois P, Thibault C, Gottschalk M, Dick P, Wilson J. Antimicrobial resistance and prudent drug use for Streptococcus suis. Anim Health Res Rev. 2013;14:68\u201377.","journal-title":"Anim Health Res Rev"},{"key":"533_CR17","first-page":"237","volume":"38","author":"S Abaas","year":"1986","unstructured":"Abaas S, M\u00e5rtensson L. Streptococcus suis p\u00e5visad \u00e4ven i svenska svinbes\u00e4ttningar. Svensk Veterin\u00e4rtidning. 1986;38:237\u20138.","journal-title":"Svensk Veterin\u00e4rtidning."},{"key":"533_CR18","first-page":"1","volume":"82","author":"I Atterholm","year":"1985","unstructured":"Atterholm I, Christensen P, Dahlquist E, Kronvall G. Ett fall av Streptococcus suis-meningit\u2014en ny yrkessjukdom i Sverige? L\u00e4kartidningen. 1985;82:1.","journal-title":"L\u00e4kartidningen."},{"key":"533_CR19","doi-asserted-by":"publisher","first-page":"e002105","DOI":"10.1099\/jmmcr.0.002105","volume":"1","author":"A Dalsj\u00f6","year":"2014","unstructured":"Dalsj\u00f6 A, Nilsson AC, Ramussen M. Complicated infection caused by Streptococcus suis serotype 14 transmitted from a wild boar. JMM Case Rep. 2014;1:e002105.","journal-title":"JMM Case Rep."},{"key":"533_CR20","doi-asserted-by":"publisher","first-page":"489","DOI":"10.3201\/eid2003.130535","volume":"20","author":"C Gustavsson","year":"2014","unstructured":"Gustavsson C, Rasmussen M. Septic arthritis caused by Streptococcus suis serotype 5 in pig farmer. Emerg Infect Dis. 2014;20:489\u201390.","journal-title":"Emerg Infect Dis"},{"key":"533_CR21","doi-asserted-by":"crossref","first-page":"253","DOI":"10.1186\/BF03548488","volume":"38","author":"AM Dalin","year":"1997","unstructured":"Dalin AM, Gidlund K, Eliasson-Selling L. Post-mortem examination of genital organs from sows with reproductive disturbances in a sow-pool. Acta Vet Scand. 1997;38:253\u201362.","journal-title":"Acta Vet Scand"},{"key":"533_CR22","unstructured":"Djurskyddsf\u00f6rordning (SFS 2019:66) Stockholm: N\u00e4ringsdepartementet."},{"key":"533_CR23","unstructured":"National Veterinary Institute. Surveillance of infectious diseases in animals and humans in Sweden 2018. Uppsala, Sweden: National Veterinary Institute (SVA). Uppsala, Sweden. SVA:s rapportserie 56; ISSN 1654-7098."},{"key":"533_CR24","volume-title":"R: a language and environment for statistical computing","author":"R Core Team","year":"2019","unstructured":"R Core Team. R: a language and environment for statistical computing. Vienna: R Foundation for Statistical Computing; 2019."},{"key":"533_CR25","unstructured":"Statens jordbruksverks f\u00f6reskrifter och allm\u00e4nna r\u00e5d om grish\u00e5llning inom lantbruket m.m. (SJVFS 2019:20) J\u00f6nk\u00f6ping: Jordbruksverket."},{"key":"533_CR26","doi-asserted-by":"publisher","first-page":"160","DOI":"10.1099\/00207713-37-2-160","volume":"37","author":"R Kilpper-B\u00e4lz","year":"1987","unstructured":"Kilpper-B\u00e4lz R, Schleifer KH. Streptococcus suis sp. nov., nom. rev. Int J Syst Bacteriol. 1987;37:160\u20132.","journal-title":"Int J Syst Bacteriol."},{"key":"533_CR27","doi-asserted-by":"publisher","first-page":"202","DOI":"10.3389\/fpubh.2015.00202","volume":"3","author":"M Perez-Sancho","year":"2015","unstructured":"Perez-Sancho M, Vela AI, Garcia-Seco T, Gottschalk M, Dominguez L, Fernandez-Garayzabal JF. Assessment of MALDI-TOF MS as alternative tool for Streptococcus suis identification. Front Public Health. 2015;3:202.","journal-title":"Front Public Health."},{"key":"533_CR28","doi-asserted-by":"publisher","first-page":"1834","DOI":"10.1128\/JCM.02654-12","volume":"51","author":"B Schulthess","year":"2013","unstructured":"Schulthess B, Brodner K, Bloemberg GV, Zbinden R, Bttger EC, Hombach M. Identification of gram-positive cocci by use of matrix-assisted laser desorption ionization-time of flight mass spectrometry: comparison of different preparation methods and implementation of a practical algorithm for routine diagnostics. J Clin Microbiol. 2013;51:1834\u201340.","journal-title":"J Clin Microbiol"},{"key":"533_CR29","doi-asserted-by":"publisher","first-page":"1475","DOI":"10.1128\/JCM.02365-10","volume":"49","author":"B Afshar","year":"2011","unstructured":"Afshar B, Broughton K, Creti R, Decheva A, Hufnagel M, Kriz P, et al. International external quality assurance for laboratory identification and typing of Streptococcus agalactiae (Group B streptococci). J Clin Microbiol. 2011;49:1475\u201382.","journal-title":"J Clin Microbiol"},{"key":"533_CR30","unstructured":"CLSI. Performance standards for antimicrobial disk and dilution susceptibility tests for bacteria isolated from animals. 4th ed. CLSI Supplement VET08. Wayne: Clinical and Laboratory Standards Institute; 2018."},{"key":"533_CR31","first-page":"733","volume":"45","author":"P Wallgren","year":"1993","unstructured":"Wallgren P, Vallgarda J. SPF husbandry\u2014presentation, definition and list of requirements. Svensk Veterin\u00e4rtidning. 1993;45:733\u20135.","journal-title":"Svensk Veterin\u00e4rtidning."},{"key":"533_CR32","unstructured":"Sj\u00f6lund M, Lindberg M, Pringle M, Harbom B, Unnerstad HE. Occurrence, serotype and antibiotic susceptibility of Streptococcus suis isolates from healthy and diseased Swedish pigs. In: Proceedings of the 25th international pig veterinary society congress. June 11\u201314; 2018; Chongqing, China. 2018; p. 672."},{"key":"533_CR33","doi-asserted-by":"publisher","first-page":"71","DOI":"10.1016\/S0378-1135(98)00228-4","volume":"63","author":"FM Aarestrup","year":"1998","unstructured":"Aarestrup FM, Rasmussen SR, Artursson K, Jensen NE. Trends in the resistance to antimicrobial agents of Streptococcus suis isolates from Denmark and Sweden. Vet Microbiol. 1998;63:71\u201380.","journal-title":"Vet Microbiol"},{"key":"533_CR34","unstructured":"Public Health Agency of Sweden and National Veterinary Institute. Swedres-Svarm 2017. Consumption of antibiotics and occurrence of antibiotic resistance in Sweden. Solna\/Uppsala ISSN 1650-6332; 2017."},{"key":"533_CR35","unstructured":"European Medicines Agency. Sales of veterinary antimicrobial agents in 31 European countries in 2017 (EMA\/294674\/2019). European Medicines Agency; 2019."},{"key":"533_CR36","unstructured":"Public Health Agency of Sweden and National Veterinary Institute. Swedres-Svarm 2014. Consumption of antibiotics and occurrence of antibiotic resistance in Sweden. Solna\/Uppsala ISSN 1650-6332; 2014."},{"key":"533_CR37","unstructured":"Public Health Agency of Sweden and National Veterinary Institute. Swedres-Svarm 2018. Consumption of antibiotics and occurrence of antibiotic resistance in Sweden. Solna\/Uppsala ISSN 1650-6332; 2018."},{"key":"533_CR38","unstructured":"CLSI. Understanding susceptibility data as a component of antimicrobial stewardship in veterinary settings. 1st ed. CLSI report VET09. Wayne: Clinical and Laboratory Standards Institute; 2019."},{"key":"533_CR39","first-page":"284303","volume":"2015","author":"C Zhang","year":"2015","unstructured":"Zhang C, Zhang Z, Song L, Fan X, Wen F, Xu S, et al. Antimicrobial resistance profile and genotypic characteristics of Streptococcus suis capsular type 2 isolated from clinical carrier sows and diseased pigs in China. Biomed Res Int. 2015;2015:284303.","journal-title":"Biomed Res Int"},{"key":"533_CR40","first-page":"145","volume":"78","author":"TC Soares","year":"2014","unstructured":"Soares TC, Paes AC, Megid J, Ribolla PE, Paduan Kdos S, Gottschalk M. Antimicrobial susceptibility of Streptococcus suis isolated from clinically healthy swine in Brazil. Can J Vet Res. 2014;78:145\u20139.","journal-title":"Can J Vet Res"},{"key":"533_CR41","doi-asserted-by":"publisher","first-page":"117","DOI":"10.1016\/j.vetmic.2017.06.002","volume":"207","author":"J Hernandez-Garcia","year":"2017","unstructured":"Hernandez-Garcia J, Wang J, Restif O, Holmes MA, Mather AE, Weinert LA, et al. Patterns of antimicrobial resistance in Streptococcus suis isolates from pigs with or without streptococcal disease in England between 2009 and 2014. Vet Microbiol. 2017;207:117\u201324.","journal-title":"Vet Microbiol"},{"key":"533_CR42","doi-asserted-by":"publisher","first-page":"5","DOI":"10.1186\/s12917-018-1732-5","volume":"15","author":"S Yongkiettrakul","year":"2019","unstructured":"Yongkiettrakul S, Maneerat K, Arechanajan B, Malila Y, Srimanote P, Gottschalk M, et al. Antimicrobial susceptibility of Streptococcus suis isolated from diseased pigs, asymptomatic pigs, and human patients in Thailand. BMC Vet Res. 2019;15:5.","journal-title":"BMC Vet Res."},{"key":"533_CR43","doi-asserted-by":"publisher","first-page":"585","DOI":"10.1016\/j.tim.2017.02.005","volume":"25","author":"M Segura","year":"2017","unstructured":"Segura M, Fittipaldi N, Calzas C, Gottschalk M. Critical Streptococcus suis virulence factors: are they all really critical? Trends Microbiol. 2017;25:585\u201399.","journal-title":"Trends Microbiol"},{"key":"533_CR44","first-page":"151","volume":"65","author":"DU Han","year":"2001","unstructured":"Han DU, Choi C, Ham HJ, Jung JH, Cho WS, Kim J, et al. Prevalence, capsular type and antimicrobial susceptibility of Streptococcus suis isolated from slaughter pigs in Korea. Can J Vet Res. 2001;65:151\u20135.","journal-title":"Can J Vet Res"},{"key":"533_CR45","first-page":"14","volume":"71","author":"C Marois","year":"2007","unstructured":"Marois C, Le Devendec L, Gottschalk M, Kobisch M. Detection and molecular typing of Streptococcus suis in tonsils from live pigs in France. Can J Vet Res. 2007;71:14\u201322.","journal-title":"Can J Vet Res"},{"key":"533_CR46","doi-asserted-by":"publisher","first-page":"7102","DOI":"10.1128\/AEM.02102-16","volume":"82","author":"X Qiu","year":"2016","unstructured":"Qiu X, Bai X, Lan R, Zheng H, Xu J. Novel capsular polysaccharide loci and new diagnostic tools for high-throughput capsular gene typing in Streptococcus suis. Appl Environ Microb. 2016;82:7102\u201312.","journal-title":"Appl Environ Microb."},{"key":"533_CR47","doi-asserted-by":"publisher","first-page":"3660","DOI":"10.1099\/ijsem.0.002204","volume":"67","author":"M Tohya","year":"2017","unstructured":"Tohya M, Arai S, Tomida J, Watanabe T, Kawamura Y, Katsumi M, et al. Defining the taxonomic status of Streptococcus suis serotype 33: the proposal for Streptococcus ruminantium sp. nov. Int J Syst Evol Microbiol. 2017;67:3660\u20135.","journal-title":"Int J Syst Evol Microbiol."},{"key":"533_CR48","unstructured":"Council Directive 91\/630\/EEC of 19 November 1991 laying down minimum standards for the protection of pigs."},{"key":"533_CR49","unstructured":"G\u00e5rd & Djurh\u00e4lsan. InterPIG Internationella rapporten 2018. G\u00e5rd & Djurh\u00e4lsan. 2019. https:\/\/www.gardochdjurhalsan.se\/wp-content\/uploads\/2019\/11\/internationella-rapporten-2018.pdf. Accessed 2 Mar 2020."}],"container-title":["Acta Veterinaria Scandinavica"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1186\/s13028-020-00533-3.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/article\/10.1186\/s13028-020-00533-3\/fulltext.html","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1186\/s13028-020-00533-3.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,8,8]],"date-time":"2024-08-08T09:17:50Z","timestamp":1723108670000},"score":1,"resource":{"primary":{"URL":"https:\/\/actavetscand.biomedcentral.com\/articles\/10.1186\/s13028-020-00533-3"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,6,24]]},"references-count":49,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2020,12]]}},"alternative-id":["533"],"URL":"https:\/\/doi.org\/10.1186\/s13028-020-00533-3","relation":{},"ISSN":["1751-0147"],"issn-type":[{"value":"1751-0147","type":"electronic"}],"subject":[],"published":{"date-parts":[[2020,6,24]]},"assertion":[{"value":"4 March 2020","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"15 June 2020","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"24 June 2020","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"The study was approved by the Ethics Committee for Animal Experimentation, Uppsala, Sweden (Dnr 5.8.18-15404). Informed consent from the farmers was obtained before the collection of samples and production data from the herds.","order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Ethics approval and consent to participate"}},{"value":"Not applicable.","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Consent for publication"}},{"value":"The authors declare that they have no competing interests.","order":3,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing interests"}}],"article-number":"36"}}