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Many canine vector-borne diseases are of zoonotic importance. This study aimed to assess the prevalence of vector-borne infections caused by <jats:italic>Dirofilaria immitis<\/jats:italic>, <jats:italic>Ehrlichia canis<\/jats:italic>, <jats:italic>Borrelia burgdorferi<\/jats:italic> sensu lato, <jats:italic>Anaplasma<\/jats:italic> spp. and <jats:italic>Leishmania infantum<\/jats:italic> in a dog kennel in Arge\u0219 County, Romania. Dog kennels are shelters for stray dogs with no officially registered owners that are gathered to be neutered and\/or boarded for national\/international adoptions by various public or private organizations. The international dog adoptions might represent a risk in the transmission of pathogens into new regions. In this context, a total number of 149 blood samples and 149 conjunctival swabs from asymptomatic kennel dogs were assessed using serology and quantitative real-time polymerase chain reaction. Antibodies against <jats:italic>B. burgdorferi<\/jats:italic> s.l. were detected in one dog (0.6%), anti-<jats:italic>Anaplasma<\/jats:italic> antibodies were found in five dogs (3.3%), while ten dogs (6.7%) tested positive for <jats:italic>D. immitis<\/jats:italic> antigen. Overall, 20.1% (30\/149) of dogs were positive for <jats:italic>L. infantum<\/jats:italic> DNA. All samples were seronegative for anti-<jats:italic>Leishmania<\/jats:italic> antibodies. When adopting dogs from this region of Romania, owners should be aware of possible infection with especially <jats:italic>L. infantum<\/jats:italic>. The travel of infected dogs may introduce the infection to areas where leishmaniasis is not present.<\/jats:p>","DOI":"10.1186\/s13028-020-00540-4","type":"journal-article","created":{"date-parts":[[2020,8,3]],"date-time":"2020-08-03T12:06:34Z","timestamp":1596456394000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":18,"title":["Detection of Leishmania infantum DNA and antibodies against Anaplasma spp., Borrelia burgdorferi s.l. and Ehrlichia canis in a dog kennel in South-Central Romania"],"prefix":"10.1186","volume":"62","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9124-2632","authenticated-orcid":false,"given":"Cristina Daniela","family":"Cazan","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Angela Monica","family":"Ionic\u0103","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ioana Adriana","family":"Matei","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gianluca","family":"D\u2019Amico","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Clara","family":"Mu\u00f1oz","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eduardo","family":"Berriatua","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mirabela Oana","family":"Dumitrache","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2020,8,3]]},"reference":[{"key":"540_CR1","doi-asserted-by":"publisher","first-page":"49","DOI":"10.1186\/1756-3305-4-49","volume":"4","author":"MJ Day","year":"2011","unstructured":"Day MJ. 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