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Both are zoonotic pathogens that could infect humans via dog bites. <jats:italic>C. canimorsus<\/jats:italic> may cause life-threatening infections in humans, whereas <jats:italic>C. cynodegmi<\/jats:italic> infections tend to be milder and more localized. Capsular serovars A\u2013C of <jats:italic>C. canimorsus<\/jats:italic> seem to be virulence-associated. Some of the <jats:italic>C. canimorsus<\/jats:italic> serovars described to date can also be detected in other <jats:italic>Capnocytophaga<\/jats:italic> species, including <jats:italic>C. cynodegmi.<\/jats:italic> The objective of this pilot study was to investigate the emergence of <jats:italic>C. canimorsus<\/jats:italic> and <jats:italic>C. cynodegmi<\/jats:italic> after birth in oral cavities of puppies and to evaluate the impact of the dam\u2019s <jats:italic>Capnocytophaga<\/jats:italic> spp. carrier status on the emergence. Ten litters, altogether 59 puppies, were included in the study. The puppies and their dams were sampled at five time points over seven weeks after whelping. Oral swab samples taken were investigated for the presence of <jats:italic>C. canimorsus<\/jats:italic> and <jats:italic>C. cynodegmi<\/jats:italic> by species-specific polymerase chain reaction (PCR), the specificity of which was verified by sequencing a selection of the PCR products. Samples that were positive in <jats:italic>Capnocytophaga<\/jats:italic> PCR reactions were also capsular-typed by PCR to gain more knowledge about the <jats:italic>Capnocytophaga<\/jats:italic> spp. present in the samples. Altogether 10.2% and 11.9% of puppies, or 20.0% and 30.0% of litters tested PCR-positive for <jats:italic>C. canimorsus<\/jats:italic> and <jats:italic>C. cynodegmi<\/jats:italic>, respectively. <jats:italic>Capnocytophaga<\/jats:italic> PCR-positive puppy samples were always positive for only <jats:italic>C. cynodegmi<\/jats:italic> or <jats:italic>C. canimorsus<\/jats:italic>, not both. Most <jats:italic>Capnocytophaga<\/jats:italic> PCR-positive puppies became positive at the age of 5 to 7 weeks. Only a minority (5\/16) of the <jats:italic>C. cynodegmi<\/jats:italic> PCR-positive dog samples were positive in capsular typing PCR, whereas all <jats:italic>C. canimorsus<\/jats:italic> PCR-positive dog samples were negative in capsular typing PCR. For all <jats:italic>Capnocytophaga<\/jats:italic> PCR-positive puppies, their dam was positive for the same <jats:italic>Capnocytophaga<\/jats:italic> species. These results suggest that puppies become colonized by <jats:italic>C. cynodegmi<\/jats:italic> or <jats:italic>C. canimorsus<\/jats:italic> from their dams at the time of deciduous teeth eruption.<\/jats:p>","DOI":"10.1186\/s13028-024-00751-z","type":"journal-article","created":{"date-parts":[[2024,7,2]],"date-time":"2024-07-02T11:02:05Z","timestamp":1719918125000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Emergence of Capnocytophaga canimorsus and Capnocytophaga cynodegmi in oral cavities of newborn puppies, a pilot study"],"prefix":"10.1186","volume":"66","author":[{"given":"Kristiina","family":"Suominen","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Silja","family":"\u00c5vall-J\u00e4\u00e4skel\u00e4inen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Inka","family":"Sallinen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anna-Maija","family":"Virtala","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0764-8294","authenticated-orcid":false,"given":"Joanna","family":"Koort","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2024,7,2]]},"reference":[{"key":"751_CR1","doi-asserted-by":"publisher","first-page":"005731","DOI":"10.1099\/ijsem.0.005731","volume":"73","author":"M Suzuki","year":"2023","unstructured":"Suzuki M, Imaoka K, Kimura M, Morikawa S, Maeda K. Capnocytophaga catalasegens sp. nov., isolated from feline oral cavities. Int J Syst Evol Microbiol. 2023;73:005731.","journal-title":"Int J Syst Evol Microbiol"},{"key":"751_CR2","doi-asserted-by":"publisher","first-page":"172","DOI":"10.1016\/j.vetmic.2010.01.001","volume":"144","author":"M Suzuki","year":"2010","unstructured":"Suzuki M, Kimura M, Imaoka K, Yamada A. Prevalence of Capnocytophaga canimorsus and Capnocytophaga cynodegmi in dogs and cats determined by using newly established species-specific PCR. Vet Microbiol. 2010;144:172\u20136.","journal-title":"Vet Microbiol"},{"key":"751_CR3","doi-asserted-by":"publisher","first-page":"437","DOI":"10.1016\/j.vetmic.2010.12.010","volume":"149","author":"SK Dilegge","year":"2011","unstructured":"Dilegge SK, Edgcomb VP, Leadbetter ER. Presence of the oral bacterium Capnocytophaga canimorsus in the tooth plaque of canines. 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The study was approved by the Viikki Campus Research Ethics Committee (2\/2016).","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Ethics approval"}},{"value":"Data have not been published previously.","order":3,"name":"Ethics","group":{"name":"EthicsHeading","label":"Prior publication"}},{"value":"Not applicable.","order":4,"name":"Ethics","group":{"name":"EthicsHeading","label":"Consent for publication"}},{"value":"The authors declare that they have no competing interests.","order":5,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing interests"}}],"article-number":"26"}}