{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,29]],"date-time":"2026-06-29T13:47:36Z","timestamp":1782740856664,"version":"3.54.5"},"reference-count":18,"publisher":"Springer Science and Business Media LLC","issue":"1","license":[{"start":{"date-parts":[[2026,6,29]],"date-time":"2026-06-29T00:00:00Z","timestamp":1782691200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"},{"start":{"date-parts":[[2026,6,29]],"date-time":"2026-06-29T00:00:00Z","timestamp":1782691200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"}],"funder":[{"DOI":"10.13039\/501100003509","name":"\u00d6rebro University","doi-asserted-by":"crossref","id":[{"id":"10.13039\/501100003509","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Acta Vet Scand"],"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    The aim of the present study was to determine whether\n                    <jats:italic>L. monocytogenes<\/jats:italic>\n                    bacteria from an invasive case of listeriosis cultured in a BacT\/ALERT FA bottle in 1999 have survived, and if so, whether their pulsed-field gel electrophoresis (PFGE) profiles have changed. In 2024, after more than 25\u00a0years in a refrigerator,\n                    <jats:italic>L. monocytogenes<\/jats:italic>\n                    was isolated in pure culture from the BacT\/ALERT FA bottle. All 100 selected isolates showed the same by PFGE profile with the restriction enzyme\n                    <jats:italic>Asc<\/jats:italic>\n                    I as the\n                    <jats:italic>L. monocytogenes<\/jats:italic>\n                    isolates did in 1999.\n                    <jats:italic>L. monocytogenes<\/jats:italic>\n                    can apparently survive for a long time in a nutrient-poor environment, and this must be taken into account by the food industry. Persistence in clinical samples has rarely been reported; however, several authors report persistence of\n                    <jats:italic>L. monocytogenes<\/jats:italic>\n                    in the food industry, with bacteria ending up in final products.\n                  <\/jats:p>","DOI":"10.1186\/s13028-026-00867-4","type":"journal-article","created":{"date-parts":[[2026,6,29]],"date-time":"2026-06-29T13:10:03Z","timestamp":1782738603000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Listeria monocytogenes isolated from a human case of invasive listeriosis survived more than 25\u00a0years in a blood culture bottle"],"prefix":"10.1186","volume":"68","author":[{"given":"Wilhelm","family":"Tham","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hannu","family":"Korkeala","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gloria Lopez","family":"Valladares","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marie-Louise","family":"Danielsson-Tham","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2026,6,29]]},"reference":[{"key":"867_CR1","doi-asserted-by":"crossref","unstructured":"Schlech WF. 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