{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T19:00:48Z","timestamp":1791313248064,"version":"4.3.1"},"reference-count":34,"publisher":"Springer Science and Business Media LLC","issue":"1","license":[{"start":{"date-parts":[[2026,1,3]],"date-time":"2026-01-03T00:00:00Z","timestamp":1767398400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0"},{"start":{"date-parts":[[2026,2,3]],"date-time":"2026-02-03T00:00:00Z","timestamp":1770076800000},"content-version":"vor","delay-in-days":31,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0"}],"funder":[{"DOI":"10.13039\/100020595","name":"National Science and Technology Council of Taiwan","doi-asserted-by":"crossref","award":["NSTC 114-2314-B-A49-045- and NSTC 114-2221-E-A49-073-MY3"],"award-info":[{"award-number":["NSTC 114-2314-B-A49-045- and NSTC 114-2221-E-A49-073-MY3"]}],"id":[{"id":"10.13039\/100020595","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100005937","name":"Cheng Hsin General Hospital Foundation","doi-asserted-by":"publisher","award":["CY11409 and CY11322"],"award-info":[{"award-number":["CY11409 and CY11322"]}],"id":[{"id":"10.13039\/501100005937","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Far Eastern Memorial Hospital National Yang Ming Chiao Tung University Joint Research Program","award":["114DN20 and 113DN22"],"award-info":[{"award-number":["114DN20 and 113DN22"]}]}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["J Biol Eng"],"DOI":"10.1186\/s13036-025-00619-4","type":"journal-article","created":{"date-parts":[[2026,1,4]],"date-time":"2026-01-04T02:15:14Z","timestamp":1767492914000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Unveiling the mechanism of abdominal and transcranial ultrasound stimulation against DSS-induced colitis based on proteomic analysis"],"prefix":"10.1186","volume":"20","author":[{"given":"Feng-Yi","family":"Yang","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Meng-Ting","family":"Wu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yi-Ju","family":"Pan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wei-Shen","family":"Su","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zih-Yun","family":"Pan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yi-Tang","family":"Lin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chung-Fu","family":"Sun","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yu-Chen","family":"Lin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2026,1,3]]},"reference":[{"key":"619_CR1","doi-asserted-by":"publisher","first-page":"1583","DOI":"10.1053\/j.gastro.2003.09.029","volume":"125","author":"T Card","year":"2003","unstructured":"Card T, Hubbard R, Logan RF. Mortality in inflammatory bowel disease: a population-based cohort study. Gastroenterology. 2003;125:1583\u201390.","journal-title":"Gastroenterology"},{"key":"619_CR2","first-page":"203","volume":"28","author":"M Carabotti","year":"2015","unstructured":"Carabotti M, Scirocco A, Maselli MA, Severi C. The gut-brain axis: interactions between enteric microbiota, central and enteric nervous systems. Ann Gastroenterol. 2015;28:203\u20139.","journal-title":"Ann Gastroenterol"},{"key":"619_CR3","doi-asserted-by":"publisher","first-page":"104714","DOI":"10.1016\/j.nbd.2019.104714","volume":"136","author":"TC Fung","year":"2020","unstructured":"Fung TC. The microbiota-immune axis as a central mediator of gut-brain communication. Neurobiol Dis. 2020;136:104714.","journal-title":"Neurobiol Dis"},{"key":"619_CR4","doi-asserted-by":"publisher","first-page":"798917","DOI":"10.3389\/fmicb.2022.798917","volume":"13","author":"Y Zhu","year":"2022","unstructured":"Zhu Y, Li Y, Zhang Q, Song Y, Wang L, Zhu Z. Interactions between intestinal microbiota and neural mitochondria: A new perspective on communicating pathway from gut to brain. Front Microbiol. 2022;13:798917.","journal-title":"Front Microbiol"},{"key":"619_CR5","doi-asserted-by":"publisher","first-page":"279","DOI":"10.1186\/s13054-016-1458-3","volume":"20","author":"PR de Jong","year":"2016","unstructured":"de Jong PR, Gonzalez-Navajas JM, Jansen NJ. The digestive tract as the origin of systemic inflammation. Crit Care. 2016;20:279.","journal-title":"Crit Care"},{"key":"619_CR6","doi-asserted-by":"publisher","first-page":"2489","DOI":"10.1038\/s41423-021-00757-x","volume":"18","author":"I Galea","year":"2021","unstructured":"Galea I. The blood-brain barrier in systemic infection and inflammation. Cell Mol Immunol. 2021;18:2489\u2013501.","journal-title":"Cell Mol Immunol"},{"key":"619_CR7","doi-asserted-by":"publisher","first-page":"201","DOI":"10.1186\/s12974-019-1580-7","volume":"16","author":"K Takahashi","year":"2019","unstructured":"Takahashi K, Nakagawasai O, Nemoto W, Odaira T, Sakuma W, Onogi H, et al. Effect of Enterococcus faecalis 2001 on colitis and depressive-like behavior in dextran sulfate sodium-treated mice: involvement of the brain-gut axis. J Neuroinflammation. 2019;16:201.","journal-title":"J Neuroinflammation"},{"key":"619_CR8","doi-asserted-by":"publisher","first-page":"1131","DOI":"10.1002\/pmic.201500174","volume":"16","author":"WY Wong","year":"2016","unstructured":"Wong WY, Lee MM, Chan BD, Kam RK, Zhang G, Lu AP, et al. Proteomic profiling of dextran sulfate sodium induced acute ulcerative colitis mice serum exosomes and their Immunomodulatory impact on macrophages. Proteomics. 2016;16:1131\u201345.","journal-title":"Proteomics"},{"key":"619_CR9","doi-asserted-by":"publisher","first-page":"3644","DOI":"10.3748\/wjg.v28.i28.3644","volume":"28","author":"Q Qi","year":"2022","unstructured":"Qi Q, Zhong R, Liu YN, Zhao C, Huang Y, Lu Y, et al. Mechanism of electroacupuncture and herb-partitioned moxibustion on ulcerative colitis animal model: A study based on proteomics. World J Gastroenterol. 2022;28:3644\u201365.","journal-title":"World J Gastroenterol"},{"key":"619_CR10","doi-asserted-by":"publisher","first-page":"1430","DOI":"10.1093\/cercor\/bhy039","volume":"29","author":"TT Chen","year":"2019","unstructured":"Chen TT, Lan TH, Yang FY. Low-Intensity pulsed ultrasound attenuates LPS-Induced neuroinflammation and memory impairment by modulation of TLR4\/NF-kappaB signaling and CREB\/BDNF expression. Cereb Cortex. 2019;29:1430\u20138.","journal-title":"Cereb Cortex"},{"key":"619_CR11","doi-asserted-by":"publisher","first-page":"565","DOI":"10.1186\/s12967-023-04377-z","volume":"21","author":"WS Su","year":"2023","unstructured":"Su WS, Wu CH, Song WS, Chen SF, Yang FY. Low-intensity pulsed ultrasound ameliorates glia-mediated inflammation and neuronal damage in experimental intracerebral hemorrhage conditions. J Transl Med. 2023;21:565.","journal-title":"J Transl Med"},{"key":"619_CR12","doi-asserted-by":"publisher","first-page":"495","DOI":"10.1016\/j.ebiom.2019.06.033","volume":"45","author":"NS Nunes","year":"2019","unstructured":"Nunes NS, Chandran P, Sundby M, Visioli F, da Costa Goncalves F, Burks SR, et al. Therapeutic ultrasound attenuates DSS-induced colitis through the cholinergic anti-inflammatory pathway. EBioMedicine. 2019;45:495\u2013510.","journal-title":"EBioMedicine"},{"key":"619_CR13","doi-asserted-by":"publisher","first-page":"121769","DOI":"10.1016\/j.lfs.2023.121769","volume":"325","author":"FY Yang","year":"2023","unstructured":"Yang FY, Chan WH, Gao CY, Zheng YT, Ke CH. Transabdominal ultrasound alleviates LPS-induced neuroinflammation by modulation of TLR4\/NF-kappaB signaling and tight junction protein expression. Life Sci. 2023;325:121769.","journal-title":"Life Sci"},{"key":"619_CR14","doi-asserted-by":"crossref","unstructured":"Gao CY, Pan YJ, Su WS, Wu CY, Chang TY, Yang FY. Abdominal ultrasound stimulation alleviates DSS-induced colitis and behavioral disorders in mice by mediating the microbiota-gut-brain axis balance. Neurotherapeutics. 2025;22:e00494.","DOI":"10.1016\/j.neurot.2024.e00494"},{"key":"619_CR15","doi-asserted-by":"publisher","first-page":"fcaf119","DOI":"10.1093\/braincomms\/fcaf119","volume":"7","author":"YC Lin","year":"2025","unstructured":"Lin YC, Pan YJ, Chang SM, Yang FY. Transcranial ultrasound stimulation ameliorates dextran sulphate sodium-induced colitis and behavioural disorders by suppressing the inflammatory response in the brain. Brain Commun. 2025;7:fcaf119.","journal-title":"Brain Commun"},{"key":"619_CR16","doi-asserted-by":"publisher","first-page":"65","DOI":"10.1186\/s12974-015-0281-0","volume":"12","author":"S Zonis","year":"2015","unstructured":"Zonis S, Pechnick RN, Ljubimov VA, Mahgerefteh M, Wawrowsky K, Michelsen KS, et al. Chronic intestinal inflammation alters hippocampal neurogenesis. J Neuroinflammation. 2015;12:65.","journal-title":"J Neuroinflammation"},{"key":"619_CR17","doi-asserted-by":"publisher","first-page":"716","DOI":"10.1136\/jech.55.10.716","volume":"55","author":"LM Kurina","year":"2001","unstructured":"Kurina LM, Goldacre MJ, Yeates D, Gill LE. Depression and anxiety in people with inflammatory bowel disease. J Epidemiol Community Health. 2001;55:716\u201320.","journal-title":"J Epidemiol Community Health"},{"key":"619_CR18","doi-asserted-by":"crossref","unstructured":"Chassaing B, Aitken JD, Malleshappa M, Vijay-Kumar M. Dextran Sulfate Sodium (DSS)-induced colitis in mice. Curr Protoc Immunol. 2014;104:15.25.1\u201315.25.14.","DOI":"10.1002\/0471142735.im1525s104"},{"key":"619_CR19","doi-asserted-by":"publisher","first-page":"1058","DOI":"10.1007\/s12264-018-0288-5","volume":"34","author":"Y Han","year":"2018","unstructured":"Han Y, Zhao T, Cheng X, Zhao M, Gong SH, Zhao YQ, et al. Cortical inflammation is increased in a DSS-Induced colitis mouse model. Neurosci Bull. 2018;34:1058\u201366.","journal-title":"Neurosci Bull"},{"key":"619_CR20","doi-asserted-by":"publisher","first-page":"263","DOI":"10.1186\/s12974-021-02317-6","volume":"18","author":"S Talley","year":"2021","unstructured":"Talley S, Valiauga R, Anderson L, Cannon AR, Choudhry MA, Campbell EM. DSS-induced inflammation in the colon drives a Proinflammatory signature in the brain that is ameliorated by prophylactic treatment with the S100A9 inhibitor paquinimod. J Neuroinflammation. 2021;18:263.","journal-title":"J Neuroinflammation"},{"key":"619_CR21","doi-asserted-by":"publisher","first-page":"1087","DOI":"10.1126\/science.abi6087","volume":"374","author":"G Agirman","year":"2021","unstructured":"Agirman G, Yu KB, Hsiao EY. Signaling inflammation across the gut-brain axis. Science. 2021;374:1087\u201392.","journal-title":"Science"},{"key":"619_CR22","doi-asserted-by":"publisher","first-page":"894824","DOI":"10.3389\/fnagi.2022.894824","volume":"14","author":"J Luo","year":"2022","unstructured":"Luo J, Chen L, Huang X, Xie J, Zou C, Pan M, et al. REPS1 as a potential biomarker in alzheimer\u2019s disease and vascular dementia. Front Aging Neurosci. 2022;14:894824.","journal-title":"Front Aging Neurosci"},{"key":"619_CR23","doi-asserted-by":"publisher","first-page":"2145","DOI":"10.1073\/pnas.0305170101","volume":"101","author":"SJ Hong","year":"2004","unstructured":"Hong SJ, Li H, Becker KG, Dawson VL, Dawson TM. Identification and analysis of plasticity-induced late-response genes. Proc Natl Acad Sci USA. 2004;101:2145\u201350.","journal-title":"Proc Natl Acad Sci USA"},{"key":"619_CR24","doi-asserted-by":"publisher","first-page":"240","DOI":"10.1152\/physiolgenomics.00242.2007","volume":"33","author":"WS Liang","year":"2008","unstructured":"Liang WS, Dunckley T, Beach TG, Grover A, Mastroeni D, Ramsey K, et al. Altered neuronal gene expression in brain regions differentially affected by alzheimer\u2019s disease: a reference data set. Physiol Genomics. 2008;33:240\u201356.","journal-title":"Physiol Genomics"},{"key":"619_CR25","doi-asserted-by":"publisher","first-page":"214","DOI":"10.1091\/mbc.e02-08-0499","volume":"14","author":"D Chen","year":"2003","unstructured":"Chen D, Toone WM, Mata J, Lyne R, Burns G, Kivinen K, et al. Global transcriptional responses of fission yeast to environmental stress. Mol Biol Cell. 2003;14:214\u201329.","journal-title":"Mol Biol Cell"},{"key":"619_CR26","doi-asserted-by":"publisher","first-page":"1584","DOI":"10.1038\/s41588-018-0238-1","volume":"50","author":"T Raj","year":"2018","unstructured":"Raj T, Li YI, Wong G, Humphrey J, Wang M, Ramdhani S, et al. Integrative transcriptome analyses of the aging brain implicate altered splicing in alzheimer\u2019s disease susceptibility. Nat Genet. 2018;50:1584\u201392.","journal-title":"Nat Genet"},{"key":"619_CR27","doi-asserted-by":"publisher","first-page":"1302","DOI":"10.1261\/rna.078879.121","volume":"27","author":"L Shkreta","year":"2021","unstructured":"Shkreta L, Delannoy A, Salvetti A, Chabot B. SRSF10: an atypical splicing regulator with critical roles in stress response, organ development, and viral replication. RNA. 2021;27:1302\u201317.","journal-title":"RNA"},{"key":"619_CR28","doi-asserted-by":"publisher","first-page":"1025","DOI":"10.1016\/0092-8674(94)90380-8","volume":"76","author":"B Derijard","year":"1994","unstructured":"Derijard B, Hibi M, Wu IH, Barrett T, Su B, Deng T, et al. JNK1: a protein kinase stimulated by UV light and Ha-Ras that binds and phosphorylates the c-Jun activation domain. Cell. 1994;76:1025\u201337.","journal-title":"Cell"},{"key":"619_CR29","doi-asserted-by":"publisher","first-page":"1812","DOI":"10.1126\/science.278.5344.1812","volume":"278","author":"MW Mayo","year":"1997","unstructured":"Mayo MW, Wang CY, Cogswell PC, Rogers-Graham KS, Lowe SW, Der CJ, et al. Requirement of NF-kappaB activation to suppress p53-independent apoptosis induced by oncogenic Ras. Science. 1997;278:1812\u20135.","journal-title":"Science"},{"key":"619_CR30","doi-asserted-by":"publisher","first-page":"649","DOI":"10.1016\/j.cell.2004.12.041","volume":"120","author":"H Kamata","year":"2005","unstructured":"Kamata H, Honda S, Maeda S, Chang L, Hirata H, Karin M. Reactive oxygen species promote TNFalpha-induced death and sustained JNK activation by inhibiting MAP kinase phosphatases. Cell. 2005;120:649\u201361.","journal-title":"Cell"},{"key":"619_CR31","doi-asserted-by":"publisher","first-page":"346","DOI":"10.1038\/35085597","volume":"412","author":"C Wang","year":"2001","unstructured":"Wang C, Deng L, Hong M, Akkaraju GR, Inoue J, Chen ZJ. TAK1 is a ubiquitin-dependent kinase of MKK and IKK. Nature. 2001;412:346\u201351.","journal-title":"Nature"},{"key":"619_CR32","doi-asserted-by":"publisher","first-page":"598","DOI":"10.1016\/j.molcel.2012.01.001","volume":"45","author":"X Gao","year":"2012","unstructured":"Gao X, Wang H, Yang JJ, Liu X, Liu ZR. Pyruvate kinase M2 regulates gene transcription by acting as a protein kinase. Mol Cell. 2012;45:598\u2013609.","journal-title":"Mol Cell"},{"key":"619_CR33","doi-asserted-by":"publisher","first-page":"396","DOI":"10.1016\/j.bbadis.2009.12.009","volume":"1802","author":"EK Kim","year":"2010","unstructured":"Kim EK, Choi EJ. Pathological roles of MAPK signaling pathways in human diseases. Biochim Biophys Acta. 2010;1802:396\u2013405.","journal-title":"Biochim Biophys Acta"},{"key":"619_CR34","doi-asserted-by":"publisher","first-page":"604179","DOI":"10.3389\/fimmu.2020.604179","volume":"11","author":"A Rutsch","year":"2020","unstructured":"Rutsch A, Kantsjo JB, Ronchi F. The Gut-Brain axis: how microbiota and host inflammasome influence brain physiology and pathology. Front Immunol. 2020;11:604179.","journal-title":"Front Immunol"}],"container-title":["Journal of Biological Engineering"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/article\/10.1186\/s13036-025-00619-4","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1186\/s13036-025-00619-4.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1186\/s13036-025-00619-4.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,2,3]],"date-time":"2026-02-03T11:51:52Z","timestamp":1770119512000},"score":1,"resource":{"primary":{"URL":"https:\/\/link.springer.com\/10.1186\/s13036-025-00619-4"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,1,3]]},"references-count":34,"journal-issue":{"issue":"1","published-online":{"date-parts":[[2026,12]]}},"alternative-id":["619"],"URL":"https:\/\/doi.org\/10.1186\/s13036-025-00619-4","relation":{},"ISSN":["1754-1611"],"issn-type":[{"value":"1754-1611","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,1,3]]},"assertion":[{"value":"17 September 2025","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"26 December 2025","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"3 January 2026","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Declarations"}},{"value":"Not applicable.","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Ethics and consent to participate"}},{"value":"Not applicable.","order":3,"name":"Ethics","group":{"name":"EthicsHeading","label":"Consent to publish"}},{"value":"The authors declare no competing interests.","order":4,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing interests"}}],"article-number":"25"}}