{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,4,6]],"date-time":"2025-04-06T10:06:22Z","timestamp":1743933982929,"version":"3.37.3"},"reference-count":15,"publisher":"Springer Science and Business Media LLC","issue":"1","license":[{"start":{"date-parts":[[2023,8,13]],"date-time":"2023-08-13T00:00:00Z","timestamp":1691884800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"},{"start":{"date-parts":[[2023,8,13]],"date-time":"2023-08-13T00:00:00Z","timestamp":1691884800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Mol Cytogenet"],"abstract":"<jats:title>Abstract<\/jats:title><jats:sec>\n                <jats:title>Background<\/jats:title>\n                <jats:p>High expression of the Cytokine Receptor-Like Factor 2 (<jats:italic>CRLF2<\/jats:italic>) gene has been observed in patients with acute lymphoblastic leukemia BCR-ABL1-like subtype. Currently, there is no commercial system available for the direct detection of the <jats:italic>IGH::CRLF2<\/jats:italic> fusion by fluorescent in situ hybridization (FISH), as there are for many other leukemia-related gene fusions. In an effort to verify the <jats:italic>IGH::CRLF2<\/jats:italic> fusion, some researchers prepare home-grown FISH probes from bacterial artificial chromosome clones flanking the <jats:italic>IGH<\/jats:italic> and <jats:italic>CRLF2<\/jats:italic> genes, which is the best alternative to confirm the fusion, however difficult to reproduce in most cytogenetic laboratories.<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Results<\/jats:title>\n                <jats:p>For the direct observation of the <jats:italic>IGH::CRLF2<\/jats:italic> gene fusion we designed a methodological approach requiring the two commercially available IGH and CRLF2 break-apart probes.<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Conclusions<\/jats:title>\n                <jats:p>Our methodological approach allows direct visualization of the <jats:italic>IGH::CRLF2<\/jats:italic> gene fusion and has the potential to be used for identification of other gene fusions.<\/jats:p>\n              <\/jats:sec>","DOI":"10.1186\/s13039-023-00652-2","type":"journal-article","created":{"date-parts":[[2023,8,13]],"date-time":"2023-08-13T06:01:15Z","timestamp":1691906475000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["A novel approach for direct detection of the IGH::CRLF2 gene fusion by fluorescent in situ hybridization"],"prefix":"10.1186","volume":"16","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7370-1092","authenticated-orcid":false,"given":"Rosa Mar\u00eda","family":"Gonz\u00e1lez-Arreola","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2283-2804","authenticated-orcid":false,"given":"Adriana","family":"Garc\u00eda-Romero","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9843-0644","authenticated-orcid":false,"given":"Mar\u00eda Teresa","family":"Maga\u00f1a-Torres","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0284-5114","authenticated-orcid":false,"given":"Juan Ram\u00f3n","family":"Gonz\u00e1lez-Garc\u00eda","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2023,8,13]]},"reference":[{"issue":"13","key":"652_CR1","doi-asserted-by":"publisher","first-page":"2688","DOI":"10.1182\/blood-2009-03-208397","volume":"114","author":"LJ Russell","year":"2009","unstructured":"Russell LJ, Capasso M, Vater I, Akasaka T, Bernard OA, Calasanz MJ, et al. Deregulated expression of cytokine receptor gene, CRLF2, is involved in lymphoid transformation in B-cell precursor acute lymphoblastic leukemia. Blood. 2009;114(13):2688\u201398.","journal-title":"Blood"},{"issue":"11","key":"652_CR2","doi-asserted-by":"publisher","first-page":"1243","DOI":"10.1038\/ng.469","volume":"41","author":"CG Mullighan","year":"2009","unstructured":"Mullighan CG, Collins-Underwood JR, Phillips LA, Loudin MG, Liu W, Zhang J, et al. Rearrangement of CRLF2 in B-progenitor- and down syndrome-associated acute lymphoblastic leukemia. Nat Genet. 2009;41(11):1243\u20136.","journal-title":"Nat Genet"},{"issue":"26","key":"652_CR3","doi-asserted-by":"publisher","first-page":"5312","DOI":"10.1182\/blood-2009-09-245944","volume":"115","author":"RC Harvey","year":"2010","unstructured":"Harvey RC, Mullighan CG, Chen IM, Wharton W, Mikhail FM, Carroll AJ, et al. Rearrangement of CRLF2 is associated with mutation of JAK kinases, alteration of IKZF1, Hispanic\/Latino ethnicity, and a poor outcome in pediatric B-progenitor acute lymphoblastic leukemia. Blood. 2010;115(26):5312\u201321.","journal-title":"Blood"},{"issue":"25","key":"652_CR4","doi-asserted-by":"publisher","first-page":"3100","DOI":"10.1200\/JCO.2011.40.3907","volume":"30","author":"AV Moorman","year":"2012","unstructured":"Moorman AV, Schwab C, Ensor HM, Russell LJ, Morrison H, Jones L, et al. IGH@ translocations, CRLF2 deregulation, and microdeletions in adolescents and adults with acute lymphoblastic leukemia. J Clin Oncol. 2012;30(25):3100\u20138.","journal-title":"J Clin Oncol"},{"issue":"15","key":"652_CR5","doi-asserted-by":"publisher","first-page":"3512","DOI":"10.1182\/blood-2011-11-394221","volume":"119","author":"IM Chen","year":"2012","unstructured":"Chen IM, Harvey RC, Mullighan CG, Gastier-Foster J, Wharton W, Kang H, et al. Outcome modeling with CRLF2, IKZF1, JAK, and minimal residual disease in pediatric acute lymphoblastic leukemia: a Children\u2019s Oncology Group study. Blood. 2012;119(15):3512\u201322.","journal-title":"Blood"},{"issue":"4","key":"652_CR6","doi-asserted-by":"publisher","first-page":"357","DOI":"10.1093\/ajcp\/aqx005","volume":"147","author":"S Konoplev","year":"2017","unstructured":"Konoplev S, Lu X, Konopleva M, Jain N, Ouyang J, Goswami M, et al. CRLF2-positive B-cell acute lymphoblastic leukemia in adult patients: a single-institution experience. Am J Clin Pathol. 2017;147(4):357\u201363.","journal-title":"Am J Clin Pathol"},{"issue":"4","key":"652_CR7","doi-asserted-by":"publisher","first-page":"394","DOI":"10.1200\/JCO.2016.69.0073","volume":"35","author":"KG Roberts","year":"2017","unstructured":"Roberts KG, Gu Z, Payne-Turner D, McCastlain K, Harvey RC, Chen IM, et al. High frequency and poor outcome of Philadelphia chromosome-like acute lymphoblastic leukemia in adults. J Clin Oncol. 2017;35(4):394\u2013401.","journal-title":"J Clin Oncol"},{"key":"652_CR8","doi-asserted-by":"crossref","unstructured":"Schm\u00e4h J, Fedders B, Panzer-Gr\u00fcmayer R, Fischer S, Zimmermann M, Dagdan E, et al. Molecular characterization of acute lymphoblastic leukemia with high CRLF2 gene expression in childhood. Pediatr Blood Cancer. 2017;64(10).","DOI":"10.1002\/pbc.26539"},{"issue":"1","key":"652_CR9","doi-asserted-by":"publisher","first-page":"130","DOI":"10.3324\/haematol.2015.136366","volume":"102","author":"T Herold","year":"2017","unstructured":"Herold T, Schneider S, Metzeler KH, Neumann M, Hartmann L, Roberts KG, et al. Adults with Philadelphia chromosome-like acute lymphoblastic leukemia frequently have IGH-CRLF2 and JAK2 mutations, persistence of minimal residual disease and poor prognosis. Haematologica. 2017;102(1):130\u20138.","journal-title":"Haematologica"},{"issue":"12","key":"652_CR10","doi-asserted-by":"publisher","first-page":"e514","DOI":"10.3324\/haematol.2016.161000","volume":"102","author":"N Jain","year":"2017","unstructured":"Jain N, Lu X, Daver N, Thakral B, Wang SA, Konoplev S, et al. Co-occurrence of CRLF2-rearranged and Ph+ acute lymphoblastic leukemia: a report of four patients. Haematologica. 2017;102(12):e514\u20137.","journal-title":"Haematologica"},{"issue":"4","key":"652_CR11","doi-asserted-by":"publisher","first-page":"e207","DOI":"10.1097\/MPH.0000000000000691","volume":"39","author":"AJ Robin","year":"2017","unstructured":"Robin AJ, Peterson JF, Grignon JW Jr, Gheorghe G, Burke MJ, vanTuinen P. Identification of high-risk cryptic CRLF2 rearrangements in B-cell acute lymphoblastic leukemia utilizing an FGFR3\/IGH dual-color dual-fusion DNA probe set. J Pediatr Hematol Oncol. 2017;39(4):e207\u201310.","journal-title":"J Pediatr Hematol Oncol"},{"issue":"25","key":"652_CR12","doi-asserted-by":"publisher","first-page":"3352","DOI":"10.1182\/blood-2016-12-758979","volume":"129","author":"SC Reshmi","year":"2017","unstructured":"Reshmi SC, Harvey RC, Roberts KG, Stonerock E, Smith A, Jenkins H, et al. Targetable kinase gene fusions in high-risk B-ALL: a study from the Children\u2019s Oncology Group. Blood. 2017;129(25):3352\u201361.","journal-title":"Blood"},{"issue":"8","key":"652_CR13","doi-asserted-by":"publisher","first-page":"815","DOI":"10.1182\/blood-2018-04-841676","volume":"132","author":"KG Roberts","year":"2018","unstructured":"Roberts KG, Reshmi SC, Harvey RC, Chen IM, Patel K, Stonerock E, et al. Genomic and outcome analyses of Ph-like ALL in NCI standard-risk patients: a report from the Children\u2019s Oncology Group. Blood. 2018;132(8):815\u201324.","journal-title":"Blood"},{"key":"652_CR14","doi-asserted-by":"publisher","first-page":"155896","DOI":"10.1016\/j.cyto.2022.155896","volume":"155","author":"MDR Ju\u00e1rez-Vel\u00e1zquez","year":"2022","unstructured":"Ju\u00e1rez-Vel\u00e1zquez MDR, Moreno-Lorenzana DL, Mart\u00ednez Anaya DA, Hern\u00e1ndez Monterde EA, Aguilar-Hern\u00e1ndez MM, Reyes-Le\u00f3n A, et al. High occurrence of CRLF2 abnormalities in Mexican children with B-cell acute lymphoblastic leukemia. Cytokine. 2022;155:155896.","journal-title":"Cytokine"},{"issue":"1","key":"652_CR15","doi-asserted-by":"publisher","first-page":"142","DOI":"10.1111\/ijlh.13698","volume":"44","author":"H Podgornik","year":"2022","unstructured":"Podgornik H, Doplihar Kebe A, Reber\u0161ek K, \u0160u\u0107urovi\u0107 S, \u0160kerget M, et al. Recognition of Philadelphia chromosome-like acute lymphoblastic leukemia as part of routine diagnostic work-up. Int J Lab Hematol. 2022;44(1):142\u20139.","journal-title":"Int J Lab Hematol"}],"container-title":["Molecular Cytogenetics"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1186\/s13039-023-00652-2.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/article\/10.1186\/s13039-023-00652-2\/fulltext.html","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1186\/s13039-023-00652-2.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,11,20]],"date-time":"2023-11-20T01:28:52Z","timestamp":1700443732000},"score":1,"resource":{"primary":{"URL":"https:\/\/molecularcytogenetics.biomedcentral.com\/articles\/10.1186\/s13039-023-00652-2"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,8,13]]},"references-count":15,"journal-issue":{"issue":"1","published-online":{"date-parts":[[2023,12]]}},"alternative-id":["652"],"URL":"https:\/\/doi.org\/10.1186\/s13039-023-00652-2","relation":{},"ISSN":["1755-8166"],"issn-type":[{"type":"electronic","value":"1755-8166"}],"subject":[],"published":{"date-parts":[[2023,8,13]]},"assertion":[{"value":"6 June 2023","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"25 July 2023","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"13 August 2023","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Declarations"}},{"value":"These results are derived from a research project approved by our institutional research and ethics committees (Instituto Mexicano del Seguro Social, Project # R-2021-785-028).","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Ethics approval and consent to participate"}},{"value":"Not applicable.","order":3,"name":"Ethics","group":{"name":"EthicsHeading","label":"Consent for publication"}},{"value":"The authors declare no competing interests.","order":4,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing interests"}}],"article-number":"19"}}