{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,17]],"date-time":"2026-07-17T18:00:29Z","timestamp":1784311229475,"version":"3.55.0"},"reference-count":36,"publisher":"Springer Science and Business Media LLC","issue":"1","license":[{"start":{"date-parts":[[2025,3,28]],"date-time":"2025-03-28T00:00:00Z","timestamp":1743120000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0"},{"start":{"date-parts":[[2025,3,28]],"date-time":"2025-03-28T00:00:00Z","timestamp":1743120000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0"}],"funder":[{"DOI":"10.13039\/501100013885","name":"Kementerian Kesihatan Malaysia","doi-asserted-by":"publisher","award":["NMRR-11-927-10333"],"award-info":[{"award-number":["NMRR-11-927-10333"]}],"id":[{"id":"10.13039\/501100013885","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003093","name":"Ministry of Higher Education, Malaysia","doi-asserted-by":"publisher","award":["FRGS-EC\/1\/2024\/STG01\/UITM\/02\/20"],"award-info":[{"award-number":["FRGS-EC\/1\/2024\/STG01\/UITM\/02\/20"]}],"id":[{"id":"10.13039\/501100003093","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Mol Cytogenet"],"DOI":"10.1186\/s13039-025-00709-4","type":"journal-article","created":{"date-parts":[[2025,3,29]],"date-time":"2025-03-29T05:24:29Z","timestamp":1743225869000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Genomic landscape of childhood acute lymphoblastic leukemia in Malaysia: insights from array-CGH"],"prefix":"10.1186","volume":"18","author":[{"given":"Azli","family":"Ismail","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fadly","family":"Ahid","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wong Nyuk","family":"Moi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nor Rizan","family":"Kamaluddin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ezalia","family":"Esa","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuslina Mat","family":"Yusoff","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zahidah Abu","family":"Seman","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Muhammad Asyraff","family":"Mohammed","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elizabeth","family":"George","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Asmida","family":"Isa","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zubaidah","family":"Zakaria","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2025,3,28]]},"reference":[{"issue":"1","key":"709_CR1","doi-asserted-by":"publisher","first-page":"6756","DOI":"10.1038\/s41598-020-63528-0","volume":"10","author":"A Kakaje","year":"2020","unstructured":"Kakaje A, Alhalabi MM, Ghareeb A, Karam B, Mansour B, Zahra B, et al. Rates and trends of childhood acute lymphoblastic leukaemia: an epidemiology study. Sci Rep. 2020;10(1):6756.","journal-title":"Sci Rep"},{"key":"709_CR2","unstructured":"Omar ZA, Ibrahim Tamin NS. National Cancer Registry Report 2007, Ministry of Health Malaysia. 2011."},{"issue":"6","key":"709_CR3","doi-asserted-by":"publisher","first-page":"1451","DOI":"10.1038\/s41375-022-01561-w","volume":"36","author":"F Nguyen-Khac","year":"2022","unstructured":"Nguyen-Khac F, Bidet A, Daudignon A, Lafage-Pochitaloff M, Ameye G, Bilhou-Nab\u00e9ra C, et al. The complex karyotype in hematological malignancies: a comprehensive overview by the Francophone group of hematological cytogenetics (GFCH). Leukemia. 2022;36(6):1451\u201366.","journal-title":"Leukemia"},{"issue":"1","key":"709_CR4","doi-asserted-by":"publisher","first-page":"13","DOI":"10.1111\/bjh.17879","volume":"197","author":"DTO Yeung","year":"2022","unstructured":"Yeung DTO, Osborn MP, White DL. B-cell acute lymphoblastic leukaemia: recent discoveries in molecular pathology, their prognostic significance, and a review of the current classification. Br J Haematol. 2022;197(1):13\u201327.","journal-title":"Br J Haematol"},{"issue":"1","key":"709_CR5","doi-asserted-by":"publisher","first-page":"41","DOI":"10.1186\/1755-8166-5-41","volume":"5","author":"Z Zakaria","year":"2012","unstructured":"Zakaria Z, Ahid MF, Ismail A, Keoh TS, Nor NM, Kamaluddin NR, et al. Chromosomal aberrations in ETV6\/RUNX1-positive childhood acute lymphoblastic leukemia using 244K oligonucleotide array comparative genomic hybridization. Mol Cytogenet. 2012;5(1):41.","journal-title":"Mol Cytogenet"},{"issue":"2","key":"709_CR6","doi-asserted-by":"publisher","first-page":"161","DOI":"10.1016\/j.cancergencyto.2010.04.016","volume":"200","author":"JR Kowalczyk","year":"2010","unstructured":"Kowalczyk JR, Babicz M, Gaworczyk A, Lejman M, Winnicka D, Styka B, et al. Structural and numerical abnormalities resolved in one-step analysis: the most common chromosomal rearrangements detected by comparative genomic hybridization in childhood acute lymphoblastic leukemia. Cancer Genet Cytogenet. 2010;200(2):161\u20136.","journal-title":"Cancer Genet Cytogenet"},{"issue":"4","key":"709_CR7","doi-asserted-by":"publisher","first-page":"489","DOI":"10.1016\/j.beha.2011.09.004","volume":"24","author":"CG Mullighan","year":"2011","unstructured":"Mullighan CG. Genomic profiling of B-progenitor acute lymphoblastic leukemia. Best Pract Res Clin Haematol. 2011;24(4):489\u2013503.","journal-title":"Best Pract Res Clin Haematol"},{"issue":"11","key":"709_CR8","doi-asserted-by":"publisher","first-page":"777","DOI":"10.1016\/S0929-6646(10)60123-4","volume":"109","author":"CH Pui","year":"2010","unstructured":"Pui CH. Recent research advances in childhood acute lymphoblastic leukemia. J Formos Med Association = Taiwan Yi Zhi. 2010;109(11):777\u201387.","journal-title":"J Formos Med Association = Taiwan Yi Zhi"},{"issue":"5","key":"709_CR9","doi-asserted-by":"publisher","first-page":"548","DOI":"10.1016\/j.bj.2021.02.003","volume":"44","author":"O P\u00f6s","year":"2021","unstructured":"P\u00f6s O, Radvanszky J, Bugly\u00f3 G, P\u00f6s Z, Rusnakova D, Nagy B, et al. DNA copy number variation: main characteristics, evolutionary significance, and pathological aspects. Biomedical J. 2021;44(5):548\u201359.","journal-title":"Biomedical J"},{"key":"709_CR10","doi-asserted-by":"crossref","unstructured":"Bispo JAB, Pinheiro PS, Kobetz EK. Epidemiology and etiology of leukemia and lymphoma. Cold Spring Harbor Perspect Med. 2020;10(6).","DOI":"10.1101\/cshperspect.a034819"},{"issue":"3","key":"709_CR11","doi-asserted-by":"publisher","first-page":"430","DOI":"10.1002\/pbc.22628","volume":"55","author":"DC Liang","year":"2010","unstructured":"Liang DC, Shih LY, Yang CP, Hung IJ, Liu HC, Jaing TH, et al. Frequencies of ETV6-RUNX1 fusion and hyperdiploidy in pediatric acute lymphoblastic leukemia are lower in Far East than West. Pediatr Blood Cancer. 2010;55(3):430\u20133.","journal-title":"Pediatr Blood Cancer"},{"issue":"Suppl 2","key":"709_CR12","doi-asserted-by":"publisher","first-page":"e4","DOI":"10.4081\/pr.2011.s2.e4","volume":"3","author":"C Harrison","year":"2011","unstructured":"Harrison C. New genetics and diagnosis of childhood B-cell precursor acute lymphoblastic leukemia. Pediatr Rep. 2011;3(Suppl 2):e4.","journal-title":"Pediatr Rep"},{"issue":"6","key":"709_CR13","doi-asserted-by":"publisher","first-page":"801","DOI":"10.1182\/blood.2020006996","volume":"137","author":"L Albert\u00ed-Servera","year":"2021","unstructured":"Albert\u00ed-Servera L, Demeyer S, Govaerts I, Swings T, De Bie J, Gielen O, et al. Single-cell DNA amplicon sequencing reveals clonal heterogeneity and evolution in T-cell acute lymphoblastic leukemia. Blood. 2021;137(6):801\u201311.","journal-title":"Blood"},{"key":"709_CR14","doi-asserted-by":"crossref","unstructured":"Lejman M, Cha\u0142upnik A, Chilimoniuk Z, Dobosz M. Genetic biomarkers and their clinical implications in B-Cell acute lymphoblastic leukemia in children. Int J Mol Sci [Internet]. 2022; 23(5).","DOI":"10.3390\/ijms23052755"},{"issue":"7","key":"709_CR15","doi-asserted-by":"publisher","first-page":"810","DOI":"10.1038\/ni.1617","volume":"9","author":"SJ Loughran","year":"2008","unstructured":"Loughran SJ, Kruse EA, Hacking DF, de Graaf CA, Hyland CD, Willson TA, et al. The transcription factor erg is essential for definitive hematopoiesis and the function of adult hematopoietic stem cells. Nat Immunol. 2008;9(7):810\u20139.","journal-title":"Nat Immunol"},{"issue":"7","key":"709_CR16","doi-asserted-by":"publisher","first-page":"1389","DOI":"10.1038\/s41379-020-0494-3","volume":"33","author":"W Xie","year":"2020","unstructured":"Xie W, Xu J, Hu S, Li S, Wang W, Cameron Yin C, et al. iAMP21 in acute myeloid leukemia is associated with complex karyotype, TP53 mutation and dismal outcome. Mod Pathol. 2020;33(7):1389\u201397.","journal-title":"Mod Pathol"},{"key":"709_CR17","doi-asserted-by":"crossref","unstructured":"Grimm J, Heckl D, Klusmann J-H. Molecular mechanisms of the genetic predisposition to acute megakaryoblastic leukemia in infants with down syndrome. Front Oncol. 2021;11.","DOI":"10.3389\/fonc.2021.636633"},{"key":"709_CR18","doi-asserted-by":"crossref","unstructured":"Mangaonkar AA, Patnaik MM. Hereditary Predisposition to Hematopoietic Neoplasms: When Bloodline Matters for Blood Cancers. Mayo Clinic Proceedings. 2020;95(7):1482-98.","DOI":"10.1016\/j.mayocp.2019.12.013"},{"issue":"5","key":"709_CR19","doi-asserted-by":"publisher","first-page":"812","DOI":"10.1038\/s41379-019-0423-5","volume":"33","author":"R Kiss","year":"2020","unstructured":"Kiss R, G\u00e1ng\u00f3 A, Benard-Slagter A, Egyed B, Haltrich I, Hegyi L, et al. Comprehensive profiling of disease-relevant copy number aberrations for advanced clinical diagnostics of pediatric acute lymphoblastic leukemia. Mod Pathol. 2020;33(5):812\u201324.","journal-title":"Mod Pathol"},{"issue":"2","key":"709_CR20","doi-asserted-by":"publisher","first-page":"125","DOI":"10.1016\/j.cancergencyto.2009.07.002","volume":"195","author":"AD Krstic","year":"2009","unstructured":"Krstic AD, Impera L, Guc-Scekic M, Lakic N, Djokic D, Slavkovic B, et al. A complex rearrangement involving cryptic deletion of ETV6 and CDKN1B genes in a case of childhood acute lymphoblastic leukemia. Cancer Genet Cytogenet. 2009;195(2):125\u201331.","journal-title":"Cancer Genet Cytogenet"},{"issue":"6","key":"709_CR21","doi-asserted-by":"publisher","first-page":"1258","DOI":"10.1038\/sj.leu.2404691","volume":"21","author":"RP Kuiper","year":"2007","unstructured":"Kuiper RP, Schoenmakers EF, van Reijmersdal SV, Hehir-Kwa JY, van Kessel AG, van Leeuwen FN, et al. High-resolution genomic profiling of childhood ALL reveals novel recurrent genetic lesions affecting pathways involved in lymphocyte differentiation and cell cycle progression. Leukemia. 2007;21(6):1258\u201366.","journal-title":"Leukemia"},{"issue":"7","key":"709_CR22","doi-asserted-by":"publisher","first-page":"1081","DOI":"10.3324\/haematol.2013.085175","volume":"98","author":"CJ Schwab","year":"2013","unstructured":"Schwab CJ, Chilton L, Morrison H, Jones L, Al-Shehhi H, Erhorn A, et al. Genes commonly deleted in childhood B-cell precursor acute lymphoblastic leukemia: association with cytogenetics and clinical features. Haematologica. 2013;98(7):1081\u20138.","journal-title":"Haematologica"},{"key":"709_CR23","doi-asserted-by":"publisher","first-page":"524308","DOI":"10.1155\/2012\/524308","volume":"2012","author":"F Tzifi","year":"2012","unstructured":"Tzifi F, Economopoulou C, Gourgiotis D, Ardavanis A, Papageorgiou S, Scorilas A. The role of BCL2 family of apoptosis regulator proteins in acute and chronic leukemias. Adv Hematol. 2012;2012:524308.","journal-title":"Adv Hematol"},{"issue":"1","key":"709_CR24","doi-asserted-by":"publisher","first-page":"302","DOI":"10.1038\/s41420-022-01093-3","volume":"8","author":"A Richter","year":"2022","unstructured":"Richter A, Lange S, Holz C, Brock L, Freitag T, Sekora A, et al. Effective tumor cell abrogation via Venetoclax-mediated BCL-2 Inhibition in KMT2A-rearranged acute B-lymphoblastic leukemia. Cell Death Discovery. 2022;8(1):302.","journal-title":"Cell Death Discovery"},{"issue":"1","key":"709_CR25","doi-asserted-by":"publisher","first-page":"12","DOI":"10.1186\/s43042-024-00485-7","volume":"25","author":"M Saddam","year":"2024","unstructured":"Saddam M, Paul SK, Habib MA, Fahim MA, Mimi A, Islam S, et al. Emerging biomarkers and potential therapeutics of the BCL-2 protein family: the apoptotic and anti-apoptotic context. Egypt J Med Hum Genet. 2024;25(1):12.","journal-title":"Egypt J Med Hum Genet"},{"issue":"1","key":"709_CR26","doi-asserted-by":"publisher","first-page":"19","DOI":"10.1007\/s13402-020-00572-y","volume":"44","author":"L Jones","year":"2021","unstructured":"Jones L, Naidoo M, Machado LR, Anthony K. The Duchenne muscular dystrophy gene and cancer. Cell Oncol. 2021;44(1):19\u201332.","journal-title":"Cell Oncol"},{"issue":"2","key":"709_CR27","doi-asserted-by":"publisher","first-page":"235","DOI":"10.1111\/ijlh.13369","volume":"43","author":"MS El Ashry","year":"2021","unstructured":"El Ashry MS, Elsayed GM, Madney Y, Arafah O, Allam RM, Rasekh EO. Duplication 1q is highly correlated with poor prognosis in high hyperdiploid pediatric B-acute lymphoblastic leukemia. Int J Lab Hematol. 2021;43(2):235\u201343.","journal-title":"Int J Lab Hematol"},{"issue":"2","key":"709_CR28","doi-asserted-by":"publisher","first-page":"333","DOI":"10.1038\/leu.2016.234","volume":"31","author":"CH Pui","year":"2017","unstructured":"Pui CH, Pei D, Raimondi SC, Coustan-Smith E, Jeha S, Cheng C, et al. Clinical impact of minimal residual disease in children with different subtypes of acute lymphoblastic leukemia treated with Response-Adapted therapy. Leukemia. 2017;31(2):333\u20139.","journal-title":"Leukemia"},{"issue":"1","key":"709_CR29","doi-asserted-by":"publisher","first-page":"15167","DOI":"10.1038\/s41598-023-42294-9","volume":"13","author":"T Burmeister","year":"2023","unstructured":"Burmeister T, Gr\u00f6ger D, G\u00f6kbuget N, Spriewald B, Starck M, Elmaagacli A, et al. Molecular characterization of TCF3::PBX1 chromosomal breakpoints in acute lymphoblastic leukemia and their use for measurable residual disease assessment. Sci Rep. 2023;13(1):15167.","journal-title":"Sci Rep"},{"issue":"3","key":"709_CR30","first-page":"636","volume":"37","author":"PB Sinclair","year":"2023","unstructured":"Sinclair PB, Cranston RE, Raninga P, Cheng J, Hanna R, Hawking Z, et al. Disruption to the FOXO-PRDM1 axis resulting from deletions of chromosome 6 in acute lymphoblastic leukaemia. Leukemia. 2023;37(3):636\u201349.","journal-title":"Leukemia"},{"key":"709_CR31","doi-asserted-by":"crossref","unstructured":"Solayappan M, Azlan A, Khor KZ, Yik MY, Khan M, Yusoff NM et al. Utilization of CRISPR-Mediated tools for studying functional genomics in hematological malignancies: an overview on the current perspectives, challenges, and clinical implications. Front Genet. 2022;12.","DOI":"10.3389\/fgene.2021.767298"},{"issue":"11","key":"709_CR32","doi-asserted-by":"publisher","first-page":"1590","DOI":"10.1002\/humu.24394","volume":"43","author":"MD Postel","year":"2022","unstructured":"Postel MD, Culver JO, Ricker C, Craig DW. Transcriptome analysis provides critical answers to the variants of uncertain significance conundrum. Hum Mutat. 2022;43(11):1590\u2013608.","journal-title":"Hum Mutat"},{"issue":"1","key":"709_CR33","doi-asserted-by":"publisher","first-page":"8279","DOI":"10.1038\/s41467-024-52580-3","volume":"15","author":"M Feng","year":"2024","unstructured":"Feng M, Wei X, Zheng X, Liu L, Lin L, Xia M, et al. Decoding missense variants by incorporating phase separation via machine learning. Nat Commun. 2024;15(1):8279.","journal-title":"Nat Commun"},{"key":"709_CR34","doi-asserted-by":"publisher","first-page":"571330","DOI":"10.3389\/fonc.2020.571330","volume":"10","author":"RS Grasel","year":"2020","unstructured":"Grasel RS, Felicio PS, de Paula AE, Campacci N, Garcia FAO, de Andrade ES, et al. Using Co-segregation and loss of heterozygosity analysis to define the pathogenicity of unclassified variants in hereditary breast cancer patients. Front Oncol. 2020;10:571330.","journal-title":"Front Oncol"},{"issue":"26","key":"709_CR35","doi-asserted-by":"publisher","first-page":"2726","DOI":"10.1200\/JCO.2018.78.3050","volume":"36","author":"AEJ Yeoh","year":"2018","unstructured":"Yeoh AEJ, Lu Y, Chin WHN, Chiew EKH, Lim EH, Li Z, et al. Intensifying treatment of childhood B-Lymphoblastic leukemia with IKZF1 deletion reduces relapse and improves overall survival: results of Malaysia-Singapore ALL 2010 study. J Clin Oncology: Official J Am Soc Clin Oncol. 2018;36(26):2726\u201335.","journal-title":"J Clin Oncology: Official J Am Soc Clin Oncol"},{"issue":"10","key":"709_CR36","doi-asserted-by":"publisher","first-page":"1145","DOI":"10.1093\/jnci\/djad106","volume":"115","author":"NW Fischer","year":"2023","unstructured":"Fischer NW, Ma Y-HV, Gari\u00e9py J. Emerging insights into ethnic-specific TP53 germline variants. JNCI: J Natl Cancer Inst. 2023;115(10):1145\u201356.","journal-title":"JNCI: J Natl Cancer Inst"}],"container-title":["Molecular Cytogenetics"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1186\/s13039-025-00709-4.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/article\/10.1186\/s13039-025-00709-4\/fulltext.html","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1186\/s13039-025-00709-4.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,3,29]],"date-time":"2025-03-29T05:24:46Z","timestamp":1743225886000},"score":1,"resource":{"primary":{"URL":"https:\/\/molecularcytogenetics.biomedcentral.com\/articles\/10.1186\/s13039-025-00709-4"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,3,28]]},"references-count":36,"journal-issue":{"issue":"1","published-online":{"date-parts":[[2025,12]]}},"alternative-id":["709"],"URL":"https:\/\/doi.org\/10.1186\/s13039-025-00709-4","relation":{},"ISSN":["1755-8166"],"issn-type":[{"value":"1755-8166","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,3,28]]},"assertion":[{"value":"1 April 2024","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"20 March 2025","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"28 March 2025","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Declarations"}},{"value":"The study was reviewed and approved by Medical Research and Ethics Committee (MREC), Ministry of Health, Malaysia. Written informed consent to participate in this study was not applicable for archived diagnostic samples.","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Ethics approval and consent to participate"}},{"value":"Not applicable.","order":3,"name":"Ethics","group":{"name":"EthicsHeading","label":"Consent for publication"}},{"value":"The authors declare no competing interests.","order":4,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing interests"}}],"article-number":"7"}}