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Sloan Foundation","doi-asserted-by":"publisher","award":["FG-2015-65234"],"award-info":[{"award-number":["FG-2015-65234"]}],"id":[{"id":"10.13039\/100000879","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100013873","name":"Government of Ontario","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100013873","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000038","name":"Natural Sciences and Engineering Research Council of Canada","doi-asserted-by":"publisher","award":["RGPIN-2016-06128"],"award-info":[{"award-number":["RGPIN-2016-06128"]}],"id":[{"id":"10.13039\/501100000038","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Mol Brain"],"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Parvalbumin-expressing inhibitory neurons (PV-INs) are critical for the balance and fine-tuning of complex neuronal circuits. Studies of PV-IN biology require tools for their specific labeling, targeting and manipulation. Among these, the Cre\/LoxP system is the most popular in mice, with the two commonly used PV-Cre lines cited over 5600 times. Here we report in the mouse cerebellar cortex that PV-Cre activity is not restricted to inhibitory neurons. Imaging of Cre-activated reporters demonstrated recombination in excitatory granule cells. We present evidence that PV-Cre recombination is: (1) spatially regulated and lobule specific; (2) detected in granule cells in the external and internal granule cell layers arising from strong, but transient <jats:italic>Pvalb<\/jats:italic> expression in progenitors between E13-E15; and (3) delayed in a subset of inhibitory interneurons, asynchronous with PV protein expression. Together, our findings establish the spatio-temporal patterns PV-Cre activation in the mouse cerebellum, raising considerations for conditional targeting of <jats:italic>Pvalb<\/jats:italic>-expressing inhibitory populations.<\/jats:p>","DOI":"10.1186\/s13041-022-00972-1","type":"journal-article","created":{"date-parts":[[2022,10,23]],"date-time":"2022-10-23T19:03:14Z","timestamp":1666551794000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["PV-IRES-Cre mouse line targets excitatory granule neurons in the cerebellum"],"prefix":"10.1186","volume":"15","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-6472-1075","authenticated-orcid":false,"given":"Wendy Xueyi","family":"Wang","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Julia","family":"Qiao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3110-860X","authenticated-orcid":false,"given":"Julie L.","family":"Lefebvre","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2022,10,23]]},"reference":[{"issue":"12","key":"972_CR1","doi-asserted-by":"publisher","first-page":"37","DOI":"10.3389\/fncir.2018.00037","volume":"16","author":"BR Ferguson","year":"2018","unstructured":"Ferguson BR, Gao W-J. PV interneurons: critical regulators of E\/I balance for prefrontal cortex-dependent behavior and psychiatric disorders. Front Neural Circuits. 2018;16(12):37.","journal-title":"Front Neural Circuits"},{"issue":"12","key":"972_CR2","doi-asserted-by":"publisher","first-page":"35","DOI":"10.3389\/fncir.2018.00035","volume":"4","author":"CA Villalobos","year":"2018","unstructured":"Villalobos CA, Wu Q, Lee PH, May PJ, Basso MA. Parvalbumin and GABA microcircuits in the mouse superior colliculus. Front Neural Circuits. 2018;4(12):35.","journal-title":"Front Neural Circuits"},{"issue":"11","key":"972_CR3","doi-asserted-by":"publisher","first-page":"3535","DOI":"10.1073\/pnas.1413625112","volume":"112","author":"T Kim","year":"2015","unstructured":"Kim T, Thankachan S, McKenna JT, McNally JM, Yang C, Choi JH, et al. Cortically projecting basal forebrain parvalbumin neurons regulate cortical gamma band oscillations. 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