{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,4]],"date-time":"2026-07-04T15:24:34Z","timestamp":1783178674678,"version":"3.54.6"},"reference-count":11,"publisher":"Springer Science and Business Media LLC","issue":"1","license":[{"start":{"date-parts":[[2024,5,3]],"date-time":"2024-05-03T00:00:00Z","timestamp":1714694400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"},{"start":{"date-parts":[[2024,5,3]],"date-time":"2024-05-03T00:00:00Z","timestamp":1714694400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"}],"funder":[{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"publisher","award":["20H03389"],"award-info":[{"award-number":["20H03389"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"publisher","award":["23H02638"],"award-info":[{"award-number":["23H02638"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100009619","name":"Japan Agency for Medical Research and Development","doi-asserted-by":"publisher","award":["23gm1510008h0002"],"award-info":[{"award-number":["23gm1510008h0002"]}],"id":[{"id":"10.13039\/100009619","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Mol Brain"],"abstract":"<jats:title>Abstract<\/jats:title><jats:p>We previously reported that enhanced corticotropin-releasing factor (CRF) signaling in the bed nucleus of the stria terminalis (BNST) caused the aversive responses during acute pain and suppressed the brain reward system during chronic pain. However, it remains to be examined whether chronic pain alters the excitability of CRF neurons in the BNST. In this study we investigated the chronic pain-induced changes in excitability of CRF-expressing neurons in the oval part of the BNST (ovBNST<jats:sup>CRF<\/jats:sup> neurons) by whole-cell patch-clamp electrophysiology. CRF-Cre; Ai14 mice were used to visualize CRF neurons by tdTomato. Electrophysiological recordings from brain slices prepared from a mouse model of neuropathic pain revealed that rheobase and firing threshold were significantly decreased in the chronic pain group compared with the sham-operated control group. Firing rate of the chronic pain group was higher than that of the control group. These data indicate that chronic pain elevated neuronal excitability of ovBNST<jats:sup>CRF<\/jats:sup> neurons.<\/jats:p>","DOI":"10.1186\/s13041-024-01094-6","type":"journal-article","created":{"date-parts":[[2024,5,3]],"date-time":"2024-05-03T12:02:05Z","timestamp":1714737725000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Chronic pain enhances excitability of corticotropin-releasing factor-expressing neurons in the oval part of the bed nucleus of the stria terminalis"],"prefix":"10.1186","volume":"17","author":[{"given":"Ryoko","family":"Uchida","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yasutaka","family":"Mukai","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Taiju","family":"Amano","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kenji","family":"Sakimura","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Keiichi","family":"Itoi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Akihiro","family":"Yamanaka","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0144-0679","authenticated-orcid":false,"given":"Masabumi","family":"Minami","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2024,5,3]]},"reference":[{"key":"1094_CR1","doi-asserted-by":"publisher","first-page":"5881","DOI":"10.1523\/JNEUROSCI.4278-12.2013","volume":"33","author":"S Ide","year":"2013","unstructured":"Ide S, Hara T, Ohno A, Tamano R, Koseki K, Naka T, Maruyama C, Kaneda K, Yoshioka M, Minami M. Opposing roles of corticotropin-releasing factor and neuropeptide Y within the dorsolateral bed nucleus of the stria terminalis in the negative affective component of pain in rats. J Neurosci. 2013;33:5881\u201394. https:\/\/doi.org\/10.1523\/JNEUROSCI.4278-12.2013.","journal-title":"J Neurosci"},{"key":"1094_CR2","doi-asserted-by":"publisher","first-page":"8376","DOI":"10.1523\/JNEUROSCI.3047-18.2019","volume":"39","author":"D Takahashi","year":"2019","unstructured":"Takahashi D, Asaoka Y, Kimura K, Hara R, Arakaki S, Sakasai K, Suzuki H, Yamauchi N, Nomura H, Amano T, Minami M. Tonic suppression of the mesolimbic dopaminergic system by enhanced corticotropin-releasing factor signaling within the bed nucleus of the stria terminalis in chronic pain model rats. J Neurosci. 2019;39:8376\u201385. https:\/\/doi.org\/10.1523\/JNEUROSCI.3047-18.2019.","journal-title":"J Neurosci"},{"key":"1094_CR3","doi-asserted-by":"publisher","first-page":"4054","DOI":"10.1210\/en.2014-1182","volume":"155","author":"K Itoi","year":"2014","unstructured":"Itoi K, Talukder AH, Fuse T, Kaneko T, Ozawa R, Sato T, Sugaya T, Uchida K, Yamazaki M, Abe M, Natsume R, Sakimura K. Visualization of corticotropin-releasing factor neurons by Fluorescent Proteins in the mouse brain and characterization of labeled neurons in the Paraventricular Nucleus of the Hypothalamus. Endocrinology. 2014;155:4054\u201360. https:\/\/doi.org\/10.1210\/en.2014-1182.","journal-title":"Endocrinology"},{"key":"1094_CR4","unstructured":"The Allen Mouse Brain Atlas. https:\/\/mouse.brain-map.org\/static\/atlas."},{"key":"1094_CR5","doi-asserted-by":"publisher","first-page":"741895","DOI":"10.3389\/fnmol.2021.741895","volume":"14","author":"S Ueda","year":"2021","unstructured":"Ueda S, Hosokawa M, Arikawa K, Takahashi K, Fujiwara M, Kakita M, Fukada T, Koyama H, Horigane SI, Itoi K, Kakeyama M, Matsunaga H, Takeyama H, Bito H, Takemoto-Kimura S. Distinctive regulation of emotional behaviors and fear-related gene expression responses in two extended amygdala subnuclei with similar molecular profiles. Front Mol Neurosci. 2021;14:741895. https:\/\/doi.org\/10.3389\/fnmol.2021.741895.","journal-title":"Front Mol Neurosci"},{"key":"1094_CR6","doi-asserted-by":"publisher","first-page":"149","DOI":"10.1016\/S0304-3959(00)00276-1","volume":"87","author":"I Decosterd","year":"2000","unstructured":"Decosterd I, Woolf CJ. Spared nerve injury: an animal model of persistent peripheral neuropathic pain. Pain. 2000;87:149\u201358. https:\/\/doi.org\/10.1016\/S0304-3959(00)00276-1.","journal-title":"Pain"},{"key":"1094_CR7","doi-asserted-by":"publisher","first-page":"837","DOI":"10.1016\/j.neubiorev.2004.11.001","volume":"28","author":"CL Kliethermes","year":"2005","unstructured":"Kliethermes CL. Anxiety-like behaviors following chronic ethanol exposure. 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Eur J Pain. 2012;16:61\u201371. https:\/\/doi.org\/10.1016\/j.ejpain.2011.05.016.","journal-title":"Eur J Pain"}],"container-title":["Molecular Brain"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1186\/s13041-024-01094-6.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/article\/10.1186\/s13041-024-01094-6\/fulltext.html","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1186\/s13041-024-01094-6.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,5,3]],"date-time":"2024-05-03T12:09:07Z","timestamp":1714738147000},"score":1,"resource":{"primary":{"URL":"https:\/\/molecularbrain.biomedcentral.com\/articles\/10.1186\/s13041-024-01094-6"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,5,3]]},"references-count":11,"journal-issue":{"issue":"1","published-online":{"date-parts":[[2024,12]]}},"alternative-id":["1094"],"URL":"https:\/\/doi.org\/10.1186\/s13041-024-01094-6","relation":{},"ISSN":["1756-6606"],"issn-type":[{"value":"1756-6606","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,5,3]]},"assertion":[{"value":"29 February 2024","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"25 April 2024","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"3 May 2024","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Declarations"}},{"value":"All experiments were approved by the Institutional Animal Care and Use Committees of the Research Institute of Environmental Medicine, Nagoya University, Japan (approval #19232 and #19268). All efforts were made to reduce the number of animals used and to minimize the pain and suffering of animals.","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Ethics approval and consent to participate"}},{"value":"Not applicable.","order":3,"name":"Ethics","group":{"name":"EthicsHeading","label":"Consent for publication"}},{"value":"The authors declare that they have no competing interests.","order":4,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing interests"}}],"article-number":"22"}}