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These mutations typically result in haploinsufficiency of the progranulin protein. <jats:italic>Grn<\/jats:italic><jats:sup><jats:italic>+\/\u2013<\/jats:italic><\/jats:sup> mice provide a model for progranulin haploinsufficiency and develop FTD-like behavioral abnormalities by 9\u201310 months of age. In previous work, we demonstrated that <jats:italic>Grn<\/jats:italic><jats:sup><jats:italic>+\/\u2013<\/jats:italic><\/jats:sup> mice develop a low dominance phenotype in the tube test that is associated with reduced dendritic arborization of layer II\/III pyramidal neurons in the prelimbic region of the medial prefrontal cortex (mPFC), a region key for social dominance behavior in the tube test assay. In this study, we investigated whether progranulin haploinsufficiency induced changes in dendritic spine density and morphology. Individual layer II\/III pyramidal neurons in the prelimbic mPFC of 9\u201310 month old wild-type or <jats:italic>Grn<\/jats:italic><jats:sup><jats:italic>+\/\u2013<\/jats:italic><\/jats:sup> mice were targeted for iontophoretic microinjection of fluorescent dye, followed by high-resolution confocal microscopy and 3D reconstruction for morphometry analysis. Dendritic spine density in <jats:italic>Grn<\/jats:italic><jats:sup><jats:italic>+\/\u2013<\/jats:italic><\/jats:sup> mice was comparable to wild-type littermates, but the apical dendrites in <jats:italic>Grn<\/jats:italic><jats:sup><jats:italic>+\/\u2013<\/jats:italic><\/jats:sup> mice had a shift in the proportion of spine types, with fewer stubby spines and more thin spines. Additionally, apical dendrites of <jats:italic>Grn<\/jats:italic><jats:sup><jats:italic>+\/\u2013<\/jats:italic><\/jats:sup> mice had longer spines and smaller thin spine head diameter in comparison to wild-type littermates. These changes in spine morphology may contribute to altered circuit-level activity and social dominance deficits in <jats:italic>Grn<\/jats:italic><jats:sup><jats:italic>+\/\u2013<\/jats:italic><\/jats:sup> mice.<\/jats:p>","DOI":"10.1186\/s13041-024-01095-5","type":"journal-article","created":{"date-parts":[[2024,6,5]],"date-time":"2024-06-05T12:12:57Z","timestamp":1717589577000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Dendritic spine head diameter is reduced in the prefrontal cortex of progranulin haploinsufficient mice"],"prefix":"10.1186","volume":"17","author":[{"given":"Anna K.","family":"Cook","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kelsey M.","family":"Greathouse","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Phaedra N.","family":"Manuel","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Noelle H.","family":"Cooper","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Juliana M.","family":"Eberhardt","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cameron D.","family":"Freeman","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Audrey J.","family":"Weber","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jeremy H.","family":"Herskowitz","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4706-9411","authenticated-orcid":false,"given":"Andrew E.","family":"Arrant","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2024,6,5]]},"reference":[{"issue":"7105","key":"1095_CR1","doi-asserted-by":"publisher","first-page":"916","DOI":"10.1038\/nature05016","volume":"442","author":"M Baker","year":"2006","unstructured":"Baker M, Mackenzie IR, Pickering-Brown SM, Gass J, Rademakers R, Lindholm C, et al. 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