{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,19]],"date-time":"2026-10-19T23:13:23Z","timestamp":1792451603662,"version":"4.5.0"},"reference-count":47,"publisher":"Springer Science and Business Media LLC","issue":"1","license":[{"start":{"date-parts":[[2021,4,15]],"date-time":"2021-04-15T00:00:00Z","timestamp":1618444800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2021,4,15]],"date-time":"2021-04-15T00:00:00Z","timestamp":1618444800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81802305"],"award-info":[{"award-number":["81802305"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81672735"],"award-info":[{"award-number":["81672735"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004826","name":"Natural Science Foundation of Beijing Municipality","doi-asserted-by":"publisher","award":["7192035"],"award-info":[{"award-number":["7192035"]}],"id":[{"id":"10.13039\/501100004826","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["J Exp Clin Cancer Res"],"published-print":{"date-parts":[[2021,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec>\n                <jats:title>Background<\/jats:title>\n                <jats:p>Cancer stem cell (CSC)-related chemoresistance leads to poor outcome of the patients with colorectal cancer (CRC). In this study, we identified the chemoresistance-relevant molecules and decipher the involved mechanisms to provide potential therapeutic target for CRC. We focused on Sec62, a novel target with significantly increased expression in chemoresistant CRC tissues, and further investigated its role in the progression of CRC.<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Methods<\/jats:title>\n                <jats:p>Through analyzing the differentially-expressed genes between chemoresistant and chemosensitive CRCs, we selected Sec62 as a novel chemoresistance-related target in CRC. The expression and clinical significance of Sec62 were determined by immunoblotting and immunohistochemistry in tissues and cell lines of CRC. The roles of Sec62 in drug resistance, stemness and tumorigenesis were evaluated in vitro and in vivo using functional experiments. GST pull-down, western blot, coimmunoprecipitation and Me-RIP assays were performed to further explore the downstream molecular mechanisms.<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Results<\/jats:title>\n                <jats:p>Sec62 upregulation was associated with the chemoresistance of CRC and poor outcome of CRC patients. Depletion of Sec62 sensitized CRC cells to chemotherapeutic drugs. Sec62 promoted the stemness of CRC cells through activating Wnt\/\u03b2-catenin signaling. Mechanistically, Sec62 bound to \u03b2-catenin and inhibited the degradation of \u03b2-catenin. Sec62 competitively disrupted the interaction between \u03b2-catenin and APC to inhibit the \u03b2-catenin destruction complex assembly. Moreover, Sec62 expression was upregulated by the m<jats:sup>6<\/jats:sup>A-mediated stabilization of Sec62 mRNA.<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Conclusions<\/jats:title>\n                <jats:p>Sec62 upregulated by the METTL3-mediated m<jats:sup>6<\/jats:sup>A modification promotes the stemness and chemoresistance of CRC by binding to \u03b2-catenin and enhancing Wnt signalling. Thus, m<jats:sup>6<\/jats:sup>A modification-Sec62-\u03b2-catenin molecular axis might act as therapeutic targets in improving treatment of CRC.<\/jats:p>\n              <\/jats:sec>","DOI":"10.1186\/s13046-021-01934-6","type":"journal-article","created":{"date-parts":[[2021,4,15]],"date-time":"2021-04-15T14:04:35Z","timestamp":1618495475000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":179,"title":["Sec62 promotes stemness and chemoresistance of human colorectal cancer through activating Wnt\/\u03b2-catenin pathway"],"prefix":"10.1186","volume":"40","author":[{"given":"Xiaofeng","family":"Liu","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kunqi","family":"Su","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaoyan","family":"Sun","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yang","family":"Jiang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lijun","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chenyu","family":"Hu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chunfeng","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Min","family":"Lu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaojuan","family":"Du","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Baocai","family":"Xing","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2021,4,15]]},"reference":[{"issue":"10207","key":"1934_CR1","doi-asserted-by":"publisher","first-page":"1467","DOI":"10.1016\/S0140-6736(19)32319-0","volume":"394","author":"E Dekker","year":"2019","unstructured":"Dekker E, Tanis PJ, Vleugels JLA, Kasi PM, Wallace MB. Colorectal cancer. Lancet. 2019;394(10207):1467\u201380. https:\/\/doi.org\/10.1016\/S0140-6736(19)32319-0.","journal-title":"Lancet"},{"issue":"6","key":"1934_CR2","doi-asserted-by":"publisher","first-page":"8051","DOI":"10.3892\/ol.2017.7191","volume":"14","author":"D Xu","year":"2017","unstructured":"Xu D, Liu XF, Yan XL, Wang K, Xing BC. Survival prediction in patients with resectable colorectal liver metastases: clinical risk scores and tumor response to chemotherapy. Oncol Lett. 2017;14(6):8051\u20139. https:\/\/doi.org\/10.3892\/ol.2017.7191.","journal-title":"Oncol Lett"},{"key":"1934_CR3","doi-asserted-by":"crossref","unstructured":"Siegel RL, Miller KD, Goding Sauer A, Fedewa SA, Butterly LF, Anderson JC, Cercek A, Smith RA, Jemal A. Colorectal cancer statistics, 2020. CA Cancer J Clin. 2020;","DOI":"10.3322\/caac.21601"},{"issue":"11","key":"1934_CR4","doi-asserted-by":"publisher","first-page":"780","DOI":"10.1016\/j.trecan.2017.08.007","volume":"3","author":"EK Ramos","year":"2017","unstructured":"Ramos EK, Hoffmann AD, Gerson SL, Liu H. New opportunities and challenges to defeat cancer stem cells. Trends Cancer. 2017;3(11):780\u201396. https:\/\/doi.org\/10.1016\/j.trecan.2017.08.007.","journal-title":"Trends Cancer"},{"issue":"1","key":"1934_CR5","doi-asserted-by":"publisher","first-page":"168","DOI":"10.1016\/j.canlet.2012.03.014","volume":"338","author":"S Fulda","year":"2013","unstructured":"Fulda S. Regulation of apoptosis pathways in cancer stem cells. Cancer Lett. 2013;338(1):168\u201373. https:\/\/doi.org\/10.1016\/j.canlet.2012.03.014.","journal-title":"Cancer Lett"},{"issue":"1","key":"1934_CR6","doi-asserted-by":"publisher","first-page":"422","DOI":"10.1186\/s13046-019-1392-8","volume":"38","author":"J Wang","year":"2019","unstructured":"Wang J, Xing Y, Wang Y, He Y, Wang L, Peng S, Yang L, Xie J, Li X, Qiu W, Yi Z, Liu M. A novel BMI-1 inhibitor QW24 for the treatment of stem-like colorectal cancer. J Exp Clin Cancer Res. 2019;38(1):422. https:\/\/doi.org\/10.1186\/s13046-019-1392-8.","journal-title":"J Exp Clin Cancer Res"},{"issue":"6","key":"1934_CR7","doi-asserted-by":"publisher","first-page":"985","DOI":"10.1016\/j.cell.2017.05.016","volume":"169","author":"R Nusse","year":"2017","unstructured":"Nusse R, Clevers H. Wnt\/beta-catenin signaling, disease, and emerging therapeutic modalities. Cell. 2017;169(6):985\u201399. https:\/\/doi.org\/10.1016\/j.cell.2017.05.016.","journal-title":"Cell"},{"issue":"10","key":"1934_CR8","doi-asserted-by":"publisher","first-page":"4313","DOI":"10.1096\/fj.15-273540","volume":"29","author":"C Shen","year":"2015","unstructured":"Shen C, Wang D, Liu X, Gu B, Du Y, Wei FZ, Cao LL, Song B, Lu X, Yang Q, et al. SET7\/9 regulates cancer cell proliferation by influencing beta-catenin stability. FASEB J. 2015;29(10):4313\u201323. https:\/\/doi.org\/10.1096\/fj.15-273540.","journal-title":"FASEB J"},{"key":"1934_CR9","doi-asserted-by":"publisher","first-page":"49","DOI":"10.1016\/bs.pmbts.2016.09.010","volume":"144","author":"J Deitrick","year":"2016","unstructured":"Deitrick J, Pruitt WM. Wnt\/beta catenin-mediated signaling commonly altered in colorectal cancer. Prog Mol Biol Transl Sci. 2016;144:49\u201368. https:\/\/doi.org\/10.1016\/bs.pmbts.2016.09.010.","journal-title":"Prog Mol Biol Transl Sci"},{"issue":"1","key":"1934_CR10","doi-asserted-by":"publisher","first-page":"251","DOI":"10.1186\/s13046-020-01774-w","volume":"39","author":"D Yang","year":"2020","unstructured":"Yang D, Li Q, Shang R, Yao L, Wu L, Zhang M, Zhang L, Xu M, Lu Z, Zhou J, Huang L, Huang X, Cheng D, Yang Y, Yu H. WNT4 secreted by tumor tissues promotes tumor progression in colorectal cancer by activation of the WNT\/beta-catenin signalling pathway. J Exp Clin Cancer Res. 2020;39(1):251. https:\/\/doi.org\/10.1186\/s13046-020-01774-w.","journal-title":"J Exp Clin Cancer Res"},{"issue":"6","key":"1934_CR11","doi-asserted-by":"publisher","first-page":"1981","DOI":"10.1038\/s41418-019-0475-6","volume":"27","author":"Q Luo","year":"2020","unstructured":"Luo Q, Wu X, Chang W, Zhao P, Nan Y, Zhu X, Katz JP, Su D, Liu Z. ARID1A prevents squamous cell carcinoma initiation and chemoresistance by antagonizing pRb\/E2F1\/c-Myc-mediated cancer stemness. Cell Death Differ. 2020;27(6):1981\u201397. https:\/\/doi.org\/10.1038\/s41418-019-0475-6.","journal-title":"Cell Death Differ"},{"issue":"3","key":"1934_CR12","doi-asserted-by":"publisher","first-page":"110013","DOI":"10.1098\/rsob.110013","volume":"1","author":"C Mendoza-Topaz","year":"2011","unstructured":"Mendoza-Topaz C, Mieszczanek J, Bienz M. The adenomatous polyposis coli tumour suppressor is essential for Axin complex assembly and function and opposes Axin's interaction with Dishevelled. Open Biol. 2011;1(3):110013. https:\/\/doi.org\/10.1098\/rsob.110013.","journal-title":"Open Biol"},{"issue":"3","key":"1934_CR13","doi-asserted-by":"publisher","first-page":"261","DOI":"10.1002\/humu.20078","volume":"24","author":"S Cowie","year":"2004","unstructured":"Cowie S, Drmanac S, Swanson D, Delgrosso K, Huang S, du Sart D, Drmanac R, Surrey S, Fortina P. Identification of APC gene mutations in colorectal cancer using universal microarray-based combinatorial sequencing-by-hybridization. Hum Mutat. 2004;24(3):261\u201371. https:\/\/doi.org\/10.1002\/humu.20078.","journal-title":"Hum Mutat"},{"issue":"1","key":"1934_CR14","doi-asserted-by":"publisher","first-page":"37","DOI":"10.1016\/j.molcel.2018.07.040","volume":"72","author":"L Ji","year":"2018","unstructured":"Ji L, Lu B, Wang Z, Yang Z, Reece-Hoyes J, Russ C, Xu W, Cong F. Identification of ICAT as an APC inhibitor, revealing Wnt-dependent inhibition of APC-Axin interaction. Mol Cell. 2018;72(1):37\u201347 e34. https:\/\/doi.org\/10.1016\/j.molcel.2018.07.040.","journal-title":"Mol Cell"},{"issue":"2","key":"1934_CR15","doi-asserted-by":"publisher","first-page":"269","DOI":"10.1016\/j.molcel.2015.02.018","volume":"58","author":"BJ Conti","year":"2015","unstructured":"Conti BJ, Devaraneni PK, Yang Z, David LL, Skach WR. Cotranslational stabilization of Sec62\/63 within the ER Sec61 translocon is controlled by distinct substrate-driven translocation events. Mol Cell. 2015;58(2):269\u201383. https:\/\/doi.org\/10.1016\/j.molcel.2015.02.018.","journal-title":"Mol Cell"},{"issue":"1","key":"1934_CR16","doi-asserted-by":"publisher","first-page":"17002","DOI":"10.1038\/sigtrans.2017.2","volume":"2","author":"M Linxweiler","year":"2017","unstructured":"Linxweiler M, Schick B, Zimmermann R. Let's talk about Secs: Sec61, Sec62 and Sec63 in signal transduction, oncology and personalized medicine. Signal Transduct Target Ther. 2017;2(1):17002. https:\/\/doi.org\/10.1038\/sigtrans.2017.2.","journal-title":"Signal Transduct Target Ther"},{"issue":"1","key":"1934_CR17","doi-asserted-by":"publisher","first-page":"676","DOI":"10.1186\/s12885-016-2739-6","volume":"16","author":"M Linxweiler","year":"2016","unstructured":"Linxweiler M, Bochen F, Schick B, Wemmert S, Al Kadah B, Greiner M, Hasenfus A, Bohle RM, Juhasz-Boss I, Solomayer EF, Takacs ZF. Identification of SEC62 as a potential marker for 3q amplification and cellular migration in dysplastic cervical lesions. BMC Cancer. 2016;16(1):676. https:\/\/doi.org\/10.1186\/s12885-016-2739-6.","journal-title":"BMC Cancer"},{"issue":"9","key":"1934_CR18","doi-asserted-by":"publisher","first-page":"1044","DOI":"10.1158\/2159-8290.CD-12-0592","volume":"3","author":"D Hagerstrand","year":"2013","unstructured":"Hagerstrand D, Tong A, Schumacher SE, Ilic N, Shen RR, Cheung HW, Vazquez F, Shrestha Y, Kim SY, Giacomelli AO, Rosenbluh J, Schinzel AC, Spardy NA, Barbie DA, Mermel CH, Weir BA, Garraway LA, Tamayo P, Mesirov JP, Beroukhim R, Hahn WC. Systematic interrogation of 3q26 identifies TLOC1 and SKIL as cancer drivers. Cancer Discov. 2013;3(9):1044\u201357. https:\/\/doi.org\/10.1158\/2159-8290.CD-12-0592.","journal-title":"Cancer Discov"},{"issue":"18","key":"1934_CR19","doi-asserted-by":"publisher","first-page":"9601","DOI":"10.1093\/nar\/gky777","volume":"46","author":"X Liu","year":"2018","unstructured":"Liu X, Cai S, Zhang C, Liu Z, Luo J, Xing B, Du X. Deacetylation of NAT10 by Sirt1 promotes the transition from rRNA biogenesis to autophagy upon energy stress. Nucleic Acids Res. 2018;46(18):9601\u201316. https:\/\/doi.org\/10.1093\/nar\/gky777.","journal-title":"Nucleic Acids Res"},{"issue":"1","key":"1934_CR20","doi-asserted-by":"publisher","first-page":"185","DOI":"10.1186\/s12943-019-1116-x","volume":"18","author":"Y Zhang","year":"2019","unstructured":"Zhang Y, Kang M, Zhang B, Meng F, Song J, Kaneko H, Shimamoto F, Tang B. m (6) A modification-mediated CBX8 induction regulates stemness and chemosensitivity of colon cancer via upregulation of LGR5. Mol Cancer. 2019;18(1):185. https:\/\/doi.org\/10.1186\/s12943-019-1116-x.","journal-title":"Mol Cancer"},{"issue":"1","key":"1934_CR21","doi-asserted-by":"publisher","first-page":"176","DOI":"10.1038\/nprot.2012.148","volume":"8","author":"D Dominissini","year":"2013","unstructured":"Dominissini D, Moshitch-Moshkovitz S, Salmon-Divon M, Amariglio N, Rechavi G. Transcriptome-wide mapping of N (6)-methyladenosine by m (6) A-seq based on immunocapturing and massively parallel sequencing. Nat Protoc. 2013;8(1):176\u201389. https:\/\/doi.org\/10.1038\/nprot.2012.148.","journal-title":"Nat Protoc"},{"issue":"1","key":"1934_CR22","doi-asserted-by":"publisher","first-page":"8","DOI":"10.1038\/s41392-020-0110-5","volume":"5","author":"L Yang","year":"2020","unstructured":"Yang L, Shi P, Zhao G, Xu J, Peng W, Zhang J, Zhang G, Wang X, Dong Z, Chen F, Cui H. Targeting cancer stem cell pathways for cancer therapy. Signal Transduct Target Ther. 2020;5(1):8. https:\/\/doi.org\/10.1038\/s41392-020-0110-5.","journal-title":"Signal Transduct Target Ther"},{"issue":"20","key":"1934_CR23","doi-asserted-by":"publisher","first-page":"5464","DOI":"10.1158\/0008-5472.CAN-17-0286","volume":"77","author":"L Jin","year":"2017","unstructured":"Jin L, Vu T, Yuan G, Datta PK. STRAP promotes Stemness of human colorectal cancer via epigenetic regulation of the NOTCH pathway. Cancer Res. 2017;77(20):5464\u201378. https:\/\/doi.org\/10.1158\/0008-5472.CAN-17-0286.","journal-title":"Cancer Res"},{"issue":"2","key":"1934_CR24","doi-asserted-by":"publisher","first-page":"676","DOI":"10.1038\/s41418-019-0379-5","volume":"27","author":"BR Kim","year":"2020","unstructured":"Kim BR, Na YJ, Kim JL, Jeong YA, Park SH, Jo MJ, Jeong S, Kang S, Oh SC, Lee DH. RUNX3 suppresses metastasis and stemness by inhibiting hedgehog signaling in colorectal cancer. Cell Death Differ. 2020;27(2):676\u201394. https:\/\/doi.org\/10.1038\/s41418-019-0379-5.","journal-title":"Cell Death Differ"},{"key":"1934_CR25","doi-asserted-by":"crossref","unstructured":"de Sousa EMF, Vermeulen L. Wnt signaling in cancer stem cell biology. Cancers (Basel). 2016;8","DOI":"10.3390\/cancers8070060"},{"issue":"19","key":"1934_CR26","doi-asserted-by":"publisher","first-page":"3337","DOI":"10.1242\/jcs.013771","volume":"120","author":"W Xu","year":"2007","unstructured":"Xu W, Kimelman D. Mechanistic insights from structural studies of beta-catenin and its binding partners. J Cell Sci. 2007;120(19):3337\u201344. https:\/\/doi.org\/10.1242\/jcs.013771.","journal-title":"J Cell Sci"},{"issue":"9","key":"1934_CR27","doi-asserted-by":"publisher","first-page":"1522","DOI":"10.1136\/gutjnl-2015-310625","volume":"65","author":"J Chen","year":"2016","unstructured":"Chen J, Rajasekaran M, Xia H, Zhang X, Kong SN, Sekar K, Seshachalam VP, Deivasigamani A, Goh BK, Ooi LL, et al. The microtubule-associated protein PRC1 promotes early recurrence of hepatocellular carcinoma in association with the Wnt\/beta-catenin signalling pathway. Gut. 2016;65(9):1522\u201334. https:\/\/doi.org\/10.1136\/gutjnl-2015-310625.","journal-title":"Gut"},{"issue":"4","key":"1934_CR28","doi-asserted-by":"publisher","first-page":"429","DOI":"10.1016\/j.devcel.2019.01.025","volume":"48","author":"KN Schaefer","year":"2019","unstructured":"Schaefer KN, Peifer M. Wnt\/Beta-catenin signaling regulation and a role for biomolecular condensates. Dev Cell. 2019;48(4):429\u201344. https:\/\/doi.org\/10.1016\/j.devcel.2019.01.025.","journal-title":"Dev Cell"},{"issue":"6112","key":"1934_CR29","doi-asserted-by":"publisher","first-page":"1337","DOI":"10.1126\/science.1228734","volume":"338","author":"AR Hernandez","year":"2012","unstructured":"Hernandez AR, Klein AM, Kirschner MW. Kinetic responses of beta-catenin specify the sites of Wnt control. Science. 2012;338(6112):1337\u201340. https:\/\/doi.org\/10.1126\/science.1228734.","journal-title":"Science"},{"issue":"4","key":"1934_CR30","doi-asserted-by":"publisher","first-page":"511","DOI":"10.1016\/j.molcel.2004.08.010","volume":"15","author":"NC Ha","year":"2004","unstructured":"Ha NC, Tonozuka T, Stamos JL, Choi HJ, Weis WI. Mechanism of phosphorylation-dependent binding of APC to beta-catenin and its role in beta-catenin degradation. Mol Cell. 2004;15(4):511\u201321. https:\/\/doi.org\/10.1016\/j.molcel.2004.08.010.","journal-title":"Mol Cell"},{"issue":"6","key":"1934_CR31","doi-asserted-by":"publisher","first-page":"606","DOI":"10.1016\/j.ccr.2010.10.032","volume":"18","author":"J Yeung","year":"2010","unstructured":"Yeung J, Esposito MT, Gandillet A, Zeisig BB, Griessinger E, Bonnet D, So CW. beta-catenin mediates the establishment and drug resistance of MLL leukemic stem cells. Cancer Cell. 2010;18(6):606\u201318. https:\/\/doi.org\/10.1016\/j.ccr.2010.10.032.","journal-title":"Cancer Cell"},{"key":"1934_CR32","doi-asserted-by":"publisher","first-page":"106","DOI":"10.1016\/j.canlet.2018.10.010","volume":"440-441","author":"J Zhang","year":"2019","unstructured":"Zhang J, Ren P, Xu D, Liu X, Liu Z, Zhang C, Li Y, Wang L, Du X, Xing B. Human UTP14a promotes colorectal cancer progression by forming a positive regulation loop with c-Myc. Cancer Lett. 2019;440-441:106\u201315. https:\/\/doi.org\/10.1016\/j.canlet.2018.10.010.","journal-title":"Cancer Lett"},{"issue":"7","key":"1934_CR33","doi-asserted-by":"publisher","first-page":"1187","DOI":"10.1016\/j.cell.2017.05.045","volume":"169","author":"IA Roundtree","year":"2017","unstructured":"Roundtree IA, Evans ME, Pan T, He C. Dynamic RNA modifications in gene expression regulation. Cell. 2017;169(7):1187\u2013200. https:\/\/doi.org\/10.1016\/j.cell.2017.05.045.","journal-title":"Cell"},{"issue":"3","key":"1934_CR34","doi-asserted-by":"publisher","first-page":"285","DOI":"10.1038\/s41556-018-0045-z","volume":"20","author":"H Huang","year":"2018","unstructured":"Huang H, Weng H, Sun W, Qin X, Shi H, Wu H, Zhao BS, Mesquita A, Liu C, Yuan CL, Hu YC, H\u00fcttelmaier S, Skibbe JR, Su R, Deng X, Dong L, Sun M, Li C, Nachtergaele S, Wang Y, Hu C, Ferchen K, Greis KD, Jiang X, Wei M, Qu L, Guan JL, He C, Yang J, Chen J. Recognition of RNA N (6)-methyladenosine by IGF2BP proteins enhances mRNA stability and translation. Nat Cell Biol. 2018;20(3):285\u201395. https:\/\/doi.org\/10.1038\/s41556-018-0045-z.","journal-title":"Nat Cell Biol"},{"key":"1934_CR35","doi-asserted-by":"publisher","first-page":"68","DOI":"10.1016\/j.ejca.2017.02.003","volume":"76","author":"M Del Rio","year":"2017","unstructured":"Del Rio M, Mollevi C, Bibeau F, Vie N, Selves J, Emile JF, Roger P, Gongora C, Robert J, Tubiana-Mathieu N, et al. Molecular subtypes of metastatic colorectal cancer are associated with patient response to irinotecan-based therapies. Eur J Cancer. 2017;76:68\u201375. https:\/\/doi.org\/10.1016\/j.ejca.2017.02.003.","journal-title":"Eur J Cancer"},{"issue":"1","key":"1934_CR36","doi-asserted-by":"publisher","first-page":"23","DOI":"10.1038\/tpj.2016.91","volume":"18","author":"Y Shen","year":"2018","unstructured":"Shen Y, Tong M, Liang Q, Guo Y, Sun HQ, Zheng W, Ao L, Guo Z, She F. Epigenomics alternations and dynamic transcriptional changes in responses to 5-fluorouracil stimulation reveal mechanisms of acquired drug resistance of colorectal cancer cells. Pharm J. 2018;18(1):23\u20138. https:\/\/doi.org\/10.1038\/tpj.2016.91.","journal-title":"Pharm J"},{"issue":"1","key":"1934_CR37","doi-asserted-by":"publisher","first-page":"126","DOI":"10.1038\/bjc.2011.505","volume":"106","author":"S Tsuji","year":"2012","unstructured":"Tsuji S, Midorikawa Y, Takahashi T, Yagi K, Takayama T, Yoshida K, Sugiyama Y, Aburatani H. Potential responders to FOLFOX therapy for colorectal cancer by random forests analysis. Br J Cancer. 2012;106(1):126\u201332. https:\/\/doi.org\/10.1038\/bjc.2011.505.","journal-title":"Br J Cancer"},{"issue":"11","key":"1934_CR38","doi-asserted-by":"publisher","first-page":"1173","DOI":"10.1038\/ncb3423","volume":"18","author":"F Fumagalli","year":"2016","unstructured":"Fumagalli F, Noack J, Bergmann TJ, Cebollero E, Pisoni GB, Fasana E, Fregno I, Galli C, Loi M, Solda T, et al. Translocon component Sec62 acts in endoplasmic reticulum turnover during stress recovery. Nat Cell Biol. 2016;18(11):1173\u201384. https:\/\/doi.org\/10.1038\/ncb3423.","journal-title":"Nat Cell Biol"},{"issue":"19","key":"1934_CR39","doi-asserted-by":"publisher","first-page":"4270","DOI":"10.1242\/jcs.153650","volume":"127","author":"SJ Jung","year":"2014","unstructured":"Jung SJ, Kim JE, Reithinger JH, Kim H. The Sec62-Sec63 translocon facilitates translocation of the C-terminus of membrane proteins. J Cell Sci. 2014;127(19):4270\u20138. https:\/\/doi.org\/10.1242\/jcs.153650.","journal-title":"J Cell Sci"},{"issue":"12","key":"1934_CR40","doi-asserted-by":"publisher","first-page":"74","DOI":"10.1038\/s41389-019-0183-6","volume":"8","author":"J Du","year":"2019","unstructured":"Du J, Zhao Z, Zhao H, Liu D, Liu H, Chen J, Cheng B, Zhai X, Yin Z, Zhang Y, Ling C. Sec62 promotes early recurrence of hepatocellular carcinoma through activating integrinalpha\/CAV1 signalling. Oncogenesis. 2019;8(12):74. https:\/\/doi.org\/10.1038\/s41389-019-0183-6.","journal-title":"Oncogenesis"},{"issue":"5","key":"1934_CR41","doi-asserted-by":"publisher","first-page":"1405","DOI":"10.1007\/s00404-019-05081-4","volume":"299","author":"FZ Takacs","year":"2019","unstructured":"Takacs FZ, Radosa JC, Linxweiler M, Kasoha M, Bohle RM, Bochen F, Unger C, Solomayer EF, Schick B, Juhasz-Boss I. Identification of 3q oncogene SEC62 as a marker for distant metastasis and poor clinical outcome in invasive ductal breast cancer. Arch Gynecol Obstet. 2019;299(5):1405\u201313. https:\/\/doi.org\/10.1007\/s00404-019-05081-4.","journal-title":"Arch Gynecol Obstet"},{"issue":"5","key":"1934_CR42","doi-asserted-by":"publisher","first-page":"716","DOI":"10.1016\/j.molcel.2014.05.015","volume":"54","author":"H Easwaran","year":"2014","unstructured":"Easwaran H, Tsai HC, Baylin SB. Cancer epigenetics: tumor heterogeneity, plasticity of stem-like states, and drug resistance. Mol Cell. 2014;54(5):716\u201327. https:\/\/doi.org\/10.1016\/j.molcel.2014.05.015.","journal-title":"Mol Cell"},{"issue":"2","key":"1934_CR43","doi-asserted-by":"publisher","first-page":"227","DOI":"10.1111\/his.13227","volume":"71","author":"H Takahashi","year":"2017","unstructured":"Takahashi H, Nakamura K, Usami A, Tsuruta T, Hashimura M, Matsumoto T, Saegusa M. Possible role of nuclear beta-catenin in resistance to preoperative chemoradiotherapy in locally advanced rectal cancer. Histopathology. 2017;71(2):227\u201337. https:\/\/doi.org\/10.1111\/his.13227.","journal-title":"Histopathology"},{"issue":"8","key":"1934_CR44","doi-asserted-by":"publisher","first-page":"4085","DOI":"10.21873\/anticanres.11795","volume":"37","author":"S Urushibara","year":"2017","unstructured":"Urushibara S, Tsubota T, Asai R, Azumi J, Ashida K, Fujiwara Y, Shiota G. WNT\/beta-catenin signaling inhibitor IC-2 suppresses sphere formation and sensitizes colorectal cancer cells to 5-fluorouracil. Anticancer Res. 2017;37(8):4085\u201391. https:\/\/doi.org\/10.21873\/anticanres.11795.","journal-title":"Anticancer Res"},{"issue":"12","key":"1934_CR45","doi-asserted-by":"publisher","first-page":"9538","DOI":"10.1002\/jcp.26874","volume":"233","author":"FK Dermani","year":"2018","unstructured":"Dermani FK, Amini R, Saidijam M, Pourjafar M, Saki S, Najafi R. Zerumbone inhibits epithelial-mesenchymal transition and cancer stem cells properties by inhibiting the beta-catenin pathway through miR-200c. J Cell Physiol. 2018;233(12):9538\u201347. https:\/\/doi.org\/10.1002\/jcp.26874.","journal-title":"J Cell Physiol"},{"issue":"4","key":"1934_CR46","doi-asserted-by":"publisher","first-page":"640","DOI":"10.1016\/j.molcel.2019.04.025","volume":"74","author":"H Shi","year":"2019","unstructured":"Shi H, Wei J, He C. Where, when, and how: context-dependent functions of RNA methylation writers, readers, and erasers. Mol Cell. 2019;74(4):640\u201350. https:\/\/doi.org\/10.1016\/j.molcel.2019.04.025.","journal-title":"Mol Cell"},{"issue":"1","key":"1934_CR47","doi-asserted-by":"publisher","first-page":"112","DOI":"10.1186\/s12943-019-1038-7","volume":"18","author":"T Li","year":"2019","unstructured":"Li T, Hu PS, Zuo Z, Lin JF, Li X, Wu QN, Chen ZH, Zeng ZL, Wang F, Zheng J, Chen D, Li B, Kang TB, Xie D, Lin D, Ju HQ, Xu RH. METTL3 facilitates tumor progression via an m (6) A-IGF2BP2-dependent mechanism in colorectal carcinoma. Mol Cancer. 2019;18(1):112. https:\/\/doi.org\/10.1186\/s12943-019-1038-7.","journal-title":"Mol Cancer"}],"container-title":["Journal of Experimental &amp; Clinical Cancer Research"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1186\/s13046-021-01934-6.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/article\/10.1186\/s13046-021-01934-6\/fulltext.html","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1186\/s13046-021-01934-6.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,4,15]],"date-time":"2021-04-15T14:17:12Z","timestamp":1618496232000},"score":1,"resource":{"primary":{"URL":"https:\/\/jeccr.biomedcentral.com\/articles\/10.1186\/s13046-021-01934-6"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,4,15]]},"references-count":47,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2021,12]]}},"alternative-id":["1934"],"URL":"https:\/\/doi.org\/10.1186\/s13046-021-01934-6","relation":{},"ISSN":["1756-9966"],"issn-type":[{"value":"1756-9966","type":"electronic"}],"subject":[],"published":{"date-parts":[[2021,4,15]]},"assertion":[{"value":"30 December 2020","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"30 March 2021","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"15 April 2021","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Declarations"}},{"value":"This study was reviewed and approved by the Ethics Committee of Peking University Cancer Hospital & Institute, and written informed consent was obtained from all patients. All animal experiments were reviewed and approved by the Ethics Review Committee of Peking University Health Science Center.","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Ethics approval and consent to participate"}},{"value":"All the authors agree to the content of the paper and their being listed as a co-author of the paper.","order":3,"name":"Ethics","group":{"name":"EthicsHeading","label":"Consent for publication"}},{"value":"The authors declare no competing interests.","order":4,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing interests"}}],"article-number":"132"}}