{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,21]],"date-time":"2026-08-21T13:08:19Z","timestamp":1787317699904,"version":"build-2736575974"},"reference-count":33,"publisher":"Springer Science and Business Media LLC","issue":"1","license":[{"start":{"date-parts":[[2019,5,7]],"date-time":"2019-05-07T00:00:00Z","timestamp":1557187200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["J Ovarian Res"],"published-print":{"date-parts":[[2019,12]]},"DOI":"10.1186\/s13048-019-0513-5","type":"journal-article","created":{"date-parts":[[2019,5,7]],"date-time":"2019-05-07T13:34:06Z","timestamp":1557236046000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":31,"title":["Association of miRNA \u2212\u2009320 expression level and its target gene endothelin-1 with the susceptibility and clinical features of polycystic ovary syndrome"],"prefix":"10.1186","volume":"12","author":[{"given":"Nearmeen M.","family":"Rashad","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marwa Abdel-Monem","family":"Ateya","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yasser S.","family":"Saraya","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Walid Mohamed","family":"Elnagar","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Khaled Fathy","family":"Helal","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mohamed El-Bakry","family":"Lashin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Amr Ahmed","family":"Abdelrhman","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ayman E.","family":"Alil","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mohammed S.","family":"Yousef","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2019,5,7]]},"reference":[{"key":"513_CR1","doi-asserted-by":"publisher","first-page":"e3","DOI":"10.1017\/S1462399408000598","volume":"10","author":"E Diamanti-Kandarakis","year":"2008","unstructured":"Diamanti-Kandarakis E. Polycystic ovarian syndrome: pathophysiology, molecular aspects, and clinical implications. Expert Rev Mol Med. 2008;10:e3.","journal-title":"Expert Rev Mol Med"},{"key":"513_CR2","doi-asserted-by":"publisher","first-page":"13","DOI":"10.1385\/ENDO:30:1:13","volume":"30","author":"E Diamanti-Kandarakis","year":"2006","unstructured":"Diamanti-Kandarakis E. Insulin resistance in PCOS. Endocrine. 2006;30:13\u20137.","journal-title":"Endocrine."},{"key":"513_CR3","first-page":"774","volume":"18","author":"A Dunaif","year":"1997","unstructured":"Dunaif A. Insulin resistance and the polycystic ovary syndrome: mechanism and implications for pathogenesis. Endocr Rev. 1997;18:774\u2013800.","journal-title":"Endocr Rev"},{"key":"513_CR4","doi-asserted-by":"publisher","first-page":"354","DOI":"10.1111\/j.1467-789X.2009.00659.x","volume":"11","author":"HM Heneghan","year":"2010","unstructured":"Heneghan HM, Miller N, Kerin MJ. Role of microRNAs in obesity and the metabolic syndrome. Obes Rev. 2010;11:354\u201361.","journal-title":"Obes Rev"},{"key":"513_CR5","doi-asserted-by":"publisher","first-page":"1825","DOI":"10.2337\/db11-0171","volume":"60","author":"SL Fernandez-Valverde","year":"2011","unstructured":"Fernandez-Valverde SL, Taft RJ, Mattick JS. MicroRNAs in beta-cell biology, insulin resistance, diabetes, and its complications. Diabetes. 2011;60:1825\u201331.","journal-title":"Diabetes."},{"key":"513_CR6","doi-asserted-by":"publisher","first-page":"E29","DOI":"10.2527\/jas.2008-1331","volume":"87","author":"MZ Carletti","year":"2009","unstructured":"Carletti MZ, Christenson LK. MicroRNA in the ovary and female reproductive tract. J Anim Sci. 2009;87:E29\u201338.","journal-title":"J Anim Sci"},{"key":"513_CR7","unstructured":"Xue Y, Ly J, Xu P, Gu L, Cao J, Xu L, et al. Identification of microRNAsand genes associated with hyperandrogenismin the folicular fluid of women with polycystic ovary syndrome. J. Cell. Biochem. 2018;119(5):3913-3921."},{"issue":"7","key":"513_CR8","doi-asserted-by":"publisher","first-page":"532","DOI":"10.4251\/wjgo.v8.i7.532","volume":"8","author":"T Tadano","year":"2016","unstructured":"Tadano T, Kakuta Y, Hamada S, Shimodaira Y, Kuroha M, Kawakami Y. MicroRNA-320 family is downregulated in colorectal adenoma and affects tumor proliferation by targeting CDK6. World J Gastrointest Oncol. 2016;8(7):532\u201342. \n                    https:\/\/doi.org\/10.4251\/wjgo.v8.i7.532\n                    \n                  .","journal-title":"World J Gastrointest Oncol"},{"key":"513_CR9","doi-asserted-by":"publisher","first-page":"21","DOI":"10.1186\/s13167-015-0043-1","volume":"6","author":"J Flammer","year":"2015","unstructured":"Flammer J, Konieczka K. Retinal venous pressure: the role of endothelin. EPMA J. 2015;6:21.","journal-title":"EPMA J"},{"key":"513_CR10","doi-asserted-by":"publisher","first-page":"360","DOI":"10.1007\/s12032-012-0360-3","volume":"30","author":"C Li","year":"2003","unstructured":"Li C, Sun Y, Liu B. The relationship between endothelin and tumor. Foreign Med Oncol Branch. 2003;30:360\u20132.","journal-title":"Foreign Med Oncol Branch"},{"key":"513_CR11","doi-asserted-by":"publisher","first-page":"41","DOI":"10.1093\/humrep\/deh098","volume":"19","author":"The Rotterdam ESHRE-ASRM-Sponsored PCOS Consensus Workshop Group","year":"2004","unstructured":"The Rotterdam ESHRE-ASRM-Sponsored PCOS Consensus Workshop Group. Revised 2003 consensus on diagnostic criteria and long-term health risks related to polycystic ovary syndrome (PCOS). Hum Reprod. 2004;19:41\u20137.","journal-title":"Hum Reprod"},{"key":"513_CR12","first-page":"743","volume":"131","author":"S Mukherjee","year":"2010","unstructured":"Mukherjee S, Maitra A. Molecular and genetic factors contributing to insulin resistance in polycystic ovary syndrome. Indian J Med Res. 2010;131:743\u201360.","journal-title":"Indian J Med Res"},{"key":"513_CR13","first-page":"3984","volume":"83","author":"DS Willis","year":"1998","unstructured":"Willis DS, Watson H, Mason HD, Galea R, Brincat M, Franks S. Premature response to luteinizing hormone of granulosa cells from anovulatory women with polycystic ovary syndrome: relevance to mechanism of anovulation. J Clin Endocr Metab. 1998;83:3984\u2013399.","journal-title":"J Clin Endocr Metab"},{"key":"513_CR14","doi-asserted-by":"publisher","first-page":"823","DOI":"10.1016\/S0092-8674(01)00616-X","volume":"107","author":"V Ambros","year":"2001","unstructured":"Ambros V. MicroRNAs: Tiny regulators with great potential. Cell. 2001;107:823\u20136. \n                    https:\/\/doi.org\/10.1016\/S0092-8674(01)00616-X\n                    \n                  .","journal-title":"Cell."},{"key":"513_CR15","doi-asserted-by":"publisher","first-page":"810","DOI":"10.1161\/CIRCRESAHA.110.226357","volume":"107","author":"A Zampetaki","year":"2010","unstructured":"Zampetaki A, Kiechl S, Drozdov I, Willeit P, Mayr U, Prokopi M, Mayr A, Weger S, Oberhollenzer F, Bonora E, et al. Plasma microRNA profiling reveals loss of endothelial MiR-126 and other microRNAs in type 2 diabetes. Circ Res. 2010;107:810\u20137. \n                    https:\/\/doi.org\/10.1161\/CIRCRESAHA.110.226357\n                    \n                  .","journal-title":"Circ Res"},{"issue":"1","key":"513_CR16","doi-asserted-by":"publisher","first-page":"23","DOI":"10.1002\/iub.1457","volume":"68","author":"S Amal","year":"2016","unstructured":"Amal S, Zidan HE, Rashad NM, Abdelaziz AM, Harira MM. Association between genes encoding components of the leutinizing hormone\/luteinizing hormone\u2013choriogonadotrophin receptor pathway and polycystic ovary syndrome in Egyptian women. IUBMB Life. 2016;68(1):23\u201336.","journal-title":"IUBMB Life"},{"issue":"2","key":"513_CR17","doi-asserted-by":"publisher","first-page":"232","DOI":"10.1016\/j.jri.2012.10.007","volume":"97","author":"NM Rashad","year":"2013","unstructured":"Rashad NM, El-Shal AS, Abdelaziz AM. Association between inflammatory biomarker serum procalcitonin and obesity in women with polycystic ovary syndrome. J Reprod Immunol. 2013;97(2):232.","journal-title":"J Reprod Immunol"},{"key":"513_CR18","doi-asserted-by":"publisher","unstructured":"Rashad NM, Abd-Elrahman MA, Amal S, Amin AI. Serum visfatin as predictive marker of cardiometabolic risk in women with polycystic ovary syndrome. Middle East Fertil Soc J. 2018; \n                    https:\/\/doi.org\/10.1016\/j.mefs.2018.04.008\n                    \n                  .","DOI":"10.1016\/j.mefs.2018.04.008"},{"issue":"4","key":"513_CR19","doi-asserted-by":"publisher","first-page":"418","DOI":"10.1016\/j.mefs.2018.07.003","volume":"23","author":"NM Rashad","year":"2018","unstructured":"Rashad NM, Amin AI, Ali AE, Soliman MH. Influence of obesity on soluble endoglin and transforming growth factor b1 in women with polycystic ovary syndrome. Middle East Fertil Soc J. 2018;23(4):418\u201324.","journal-title":"Middle East Fertil Soc J"},{"issue":"11","key":"513_CR20","doi-asserted-by":"publisher","first-page":"6063","DOI":"10.1007\/s11033-013-2716-y","volume":"40","author":"S Amal","year":"2013","unstructured":"Amal S, Shalaby SM, Aly NM, Rashad NM, Abdelaziz AM. Genetic variation in the vitamin D receptor gene and vitamin D serum levels in Egyptian women with polycystic ovary syndrome. Mol Biol Rep. 2013;40(11):6063\u201373.","journal-title":"Mol Biol Rep"},{"key":"513_CR21","doi-asserted-by":"publisher","first-page":"1304","DOI":"10.1159\/000358698","volume":"33","author":"W Long","year":"2014","unstructured":"Long W, Zhao C, Ji C, Ding H, Cui Y, Guo X, Shen R, Liu J. Characterization of serum MicroRNAs profile of PCOS and identification of novel non-invasive biomarkers. Cell Physiol Biochem. 2014;33:1304\u201315 \n                    https:\/\/doi.org\/10.1159\/000358698\n                    \n                  .","journal-title":"Cell Physiol Biochem"},{"issue":"2","key":"513_CR22","doi-asserted-by":"publisher","first-page":"181","DOI":"10.1111\/j.1440-1681.2008.05057.x","volume":"36","author":"XH Wang","year":"2009","unstructured":"Wang XH, Qian RZ, Zhang W, Chen SF, Jin HM, Hu RM. MicroRNA-320 expression in myocardial microvascular endothelial cells and its relationship with insulin-like growth factor-1 in type 2 diabetic rats. Clin Exp Pharmacol Physiol. 2009;36(2):181\u20138.","journal-title":"Clin Exp Pharmacol Physiol"},{"key":"513_CR23","doi-asserted-by":"publisher","first-page":"549875","DOI":"10.5402\/2012\/549875","volume":"2012","author":"B Feng","year":"2012","unstructured":"Feng B, Chakrabarti S. miR-320 regulates glucose-induced gene expression in diabetes. ISRN Endocrinol. 2012;2012:549875.","journal-title":"ISRN Endocrinol"},{"issue":"9","key":"513_CR24","doi-asserted-by":"publisher","first-page":"e32","DOI":"10.1111\/j.1440-1681.2009.05207.x","volume":"36","author":"L HY","year":"2009","unstructured":"HY L, Ou HS, Feng SD, Zhang XY, Tuo QH, Chen LX, Zhu BY, Gao ZP, Tang CK, Yin WD, Zhang L, Liao DF. CHANGES IN microRNA (miR) profile and effects of miR-320 in insulin-resistant 3T3-L1 adipocytes. Clin Exp Pharmacol Physiol. 2009 Sep;36(9):e32\u20139. \n                    https:\/\/doi.org\/10.1111\/j.1440-1681.2009.05207.x\n                    \n                  .","journal-title":"Clin Exp Pharmacol Physiol"},{"issue":"7","key":"513_CR25","doi-asserted-by":"publisher","first-page":"3068","DOI":"10.1210\/jc.2013\u20131715","volume":"98","author":"Q Sang","year":"2013","unstructured":"Sang Q, Yao Z, Wang H, Feng R, Wang H, Zhao X, Xing Q, Jin L, He L, Wu L, Wang L. Identification of microRNAs in human follicular fluid: characterization of microRNAs that govern steroidogenesis in vitro and are associated with polycystic ovary syndrome in vivo. J Clin Endocrinol Metab. 2013;98(7):3068\u201379. \n                    https:\/\/doi.org\/10.1210\/jc.2013%2D1715\n                    \n                   Epub 2013 May 10.","journal-title":"J Clin Endocrinol Metab"},{"issue":"11","key":"513_CR26","first-page":"4946","volume":"28","author":"WN Yuan","year":"2017","unstructured":"Yuan WN, Tan L. MicroRNA-320 inhibits insulin resistance in patients with PCOS through regulating ERK1\/2 signaling pathway. Biomed Res. 2017;28(11):4946\u20139.","journal-title":"Biomed Res"},{"key":"513_CR27","doi-asserted-by":"publisher","unstructured":"Yin M, Wang X, Yao G, Lu M, Liang M, Sun Y, Sun F. Transactivation of miR-320 by miR-383 regulates granulosa cell functions by targeting E2F1 and SF-1*. J Biol Chem. 2014. \n                    https:\/\/doi.org\/10.1074\/jbc.M113.546044\n                    \n                  .","DOI":"10.1074\/jbc.M113.546044"},{"issue":"10","key":"513_CR28","doi-asserted-by":"publisher","first-page":"4666","DOI":"10.1210\/jcem.86.10.7904","volume":"86","author":"E Diamanti-Kandarakis","year":"2001","unstructured":"Diamanti-Kandarakis E, Spina G, Kouli C, Migdalis I. Increased Endothelin-1 levels in women with polycystic ovary syndrome and the beneficial effect of metformin therapy. J Clin Endocrinol Metabol. 2001;86(10):4666\u201373 \n                    https:\/\/doi.org\/10.1210\/jcem.86.10.7904\n                    \n                  .","journal-title":"J Clin Endocrinol Metabol"},{"issue":"Suppl 2","key":"513_CR29","first-page":"38","volume":"105","author":"C Ferri","year":"1997","unstructured":"Ferri C, Bellini C, Desideri G, et al. Circulating endothelin-1 levels in obese patients with the metabolic syndrome. Exp Clin Endocrinol Diabetes. 1997;105(Suppl 2):38\u201340.","journal-title":"Exp Clin Endocrinol Diabetes"},{"key":"513_CR30","doi-asserted-by":"publisher","first-page":"100","DOI":"10.1007\/s001250050649","volume":"40","author":"C Ferri","year":"1997","unstructured":"Ferri C, Bellini C, Desideri G, Baldoncini R, De Siati L, Santucci A. Elevated plasma endothelin-1 levels as an additional risk factor in non-obese essential hypertensive patients with metabolic abnormalities. Diabetologia. 1997;40:100\u20132.","journal-title":"Diabetologia"},{"key":"513_CR31","doi-asserted-by":"publisher","first-page":"1856","DOI":"10.2337\/diabetes.48.9.1856","volume":"48","author":"AE Caballero","year":"1999","unstructured":"Caballero AE, Arora S, Saouaf R, et al. Microvascular and macrovascular reactivity is reduced in subjects at a risk for type 2 diabetes. Diabetes. 1999;48:1856\u201362.","journal-title":"Diabetes"},{"issue":"5 Pt 2","key":"513_CR32","doi-asserted-by":"publisher","first-page":"17R","DOI":"10.1203\/pdr.0b013e318045760e","volume":"61","author":"P Saetrom","year":"2007","unstructured":"Saetrom P, Snove O Jr, Rossi JJ. Epigenetics and microRNAs. Pediatr Res. 2007;61(5 Pt 2):17R\u201323R. \n                    https:\/\/doi.org\/10.1203\/pdr.0b013e318045760e\n                    \n                  .","journal-title":"Pediatr Res"},{"issue":"6","key":"513_CR33","doi-asserted-by":"publisher","first-page":"1751","DOI":"10.1016\/j.fertnstert.2014.08.005","volume":"102","author":"M Santonocito","year":"2014","unstructured":"Santonocito M, Vento M, Guglielmino MR, Battaglia R, Wahlgren J, Ragusa M, et al. Molecular characterization of exosomes and their microRNA cargo in human follicular fluid: bioinformatic analysis reveals that exosomal microRNAs control pathways involved in follicular maturation. Fertil Steril. 2014;102(6):1751\u2013U590. \n                    https:\/\/doi.org\/10.1016\/j.fertnstert.2014.08.005\n                    \n                  .","journal-title":"Fertil Steril"}],"container-title":["Journal of Ovarian Research"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/link.springer.com\/content\/pdf\/10.1186\/s13048-019-0513-5.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/link.springer.com\/article\/10.1186\/s13048-019-0513-5\/fulltext.html","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/link.springer.com\/content\/pdf\/10.1186\/s13048-019-0513-5.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2020,5,5]],"date-time":"2020-05-05T19:16:57Z","timestamp":1588706217000},"score":1,"resource":{"primary":{"URL":"https:\/\/ovarianresearch.biomedcentral.com\/articles\/10.1186\/s13048-019-0513-5"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019,5,7]]},"references-count":33,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2019,12]]}},"alternative-id":["513"],"URL":"https:\/\/doi.org\/10.1186\/s13048-019-0513-5","relation":{},"ISSN":["1757-2215"],"issn-type":[{"value":"1757-2215","type":"electronic"}],"subject":[],"published":{"date-parts":[[2019,5,7]]},"assertion":[{"value":"7 February 2019","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"16 April 2019","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"7 May 2019","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"The authors declare that the research was conducted in the absence of anycommercial or financial relationships that could be construed as a potential conflict of interest.<i>In conclusion<\/i>, we found that serum miR-320 expression level were lower in PCOS patients compared to controls, in particular, PCOS women with IR. Moreover, it was negatively correlated to its target gene; ET-I as well as FSI, HOMA-IR, PCOS phenotype; hirsutism score, ovarian volume, and AFC .we hypothesis that serum miR-320 expression level could be a non-invasive biomarker of PCOS and further study indeed to assess its potential therapeutic role as suggested by previous experimental studies.","order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing interest"}},{"value":"Not applicable.","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Consent for publication"}},{"value":"The authors declare that they have no competing interests.","order":3,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing interests"}},{"value":"Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.","order":4,"name":"Ethics","group":{"name":"EthicsHeading","label":"Publisher\u2019s Note"}}],"article-number":"39"}}