{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,13]],"date-time":"2026-03-13T09:01:14Z","timestamp":1773392474487,"version":"3.50.1"},"reference-count":31,"publisher":"Springer Science and Business Media LLC","issue":"1","license":[{"start":{"date-parts":[[2021,6,21]],"date-time":"2021-06-21T00:00:00Z","timestamp":1624233600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"},{"start":{"date-parts":[[2021,6,21]],"date-time":"2021-06-21T00:00:00Z","timestamp":1624233600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"}],"funder":[{"name":"participatory approach to assess the sexual health status of rural women and a stratified intervention Study with \u201cPLA\u201das the core Ministry of Education Humanities and Social Sciences Project","award":["No:15YJCZH003"],"award-info":[{"award-number":["No:15YJCZH003"]}]}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["J Ovarian Res"],"published-print":{"date-parts":[[2021,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec>\n                <jats:title>Background<\/jats:title>\n                <jats:p>Polycystic ovary syndrome (PCOS) is a common endocrine disease in women of reproductive age. Multiple studies have shown that long non-coding RNAs (lncRNA) and microRNAs (miRNA) play a role in PCOS. This study aimed to explore the role and molecular mechanism of lncRNA -Regulator of reprogramming (lncROR) in PCOS.<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Results<\/jats:title>\n                <jats:p>Expression level of lncROR in PCOS patients was up-regulated, while level of miR-206 was down-regulated in comparison with control group (<jats:italic>P<\/jats:italic>\u2009&lt;\u20090.001). Logistics regression analysis showed that lncROR and miR-206 were independent predictors of PCOS. The ROC curve showed that lncROR had a high diagnostic value for PCOS with an AUC value of 0.893. Pearson correlation coefficient indicated that the expression level of miR-206 was negatively correlated with the level of lncROR. CCK-8 assay and apoptosis assay revealed that downregulation of lncROR up-regulated the expression of miR-206, thereby inhibiting cell proliferation and promoting cell apoptosis. However, silencing the expression of miR-206 reversed the above effects caused by down-regulation of lncROR expression. Luciferase reporter gene assay suggested that there was a target relationship between lncROR and miR-206. VEGF was proved to be the target gene of miR-206.<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Conclusions<\/jats:title>\n                <jats:p>Highly expressed lncROR indirectly up-regulated the expression of VEGF by down-regulating the expression of miR-206, thereby promoting the proliferation of KGN cells and inhibiting apoptosis, and further promoting the development of PCOS.<\/jats:p>\n              <\/jats:sec>","DOI":"10.1186\/s13048-021-00829-6","type":"journal-article","created":{"date-parts":[[2021,6,21]],"date-time":"2021-06-21T02:02:38Z","timestamp":1624240958000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Differential expression of long non-coding RNA Regulator of reprogramming and its molecular mechanisms in polycystic ovary syndrome"],"prefix":"10.1186","volume":"14","author":[{"given":"Zhihong","family":"Zhang","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Min","family":"Sang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Siqin","family":"Liu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jing","family":"Shao","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yunjiang","family":"Cai","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2021,6,21]]},"reference":[{"issue":"1","key":"829_CR1","doi-asserted-by":"publisher","first-page":"6","DOI":"10.1016\/j.fertnstert.2016.05.003","volume":"106","author":"D Lizneva","year":"2016","unstructured":"Lizneva D, et al. Criteria, prevalence, and phenotypes of polycystic ovary syndrome. Fertil Steril. 2016;106(1):6\u201315.","journal-title":"Fertil Steril"},{"issue":"4","key":"829_CR2","doi-asserted-by":"publisher","first-page":"219","DOI":"10.1038\/nrendo.2010.217","volume":"7","author":"MO Goodarzi","year":"2011","unstructured":"Goodarzi MO, et al. Polycystic ovary syndrome: etiology, pathogenesis and diagnosis. Nat Rev Endocrinol. 2011;7(4):219\u201331.","journal-title":"Nat Rev Endocrinol"},{"issue":"9588","key":"829_CR3","doi-asserted-by":"publisher","first-page":"685","DOI":"10.1016\/S0140-6736(07)61345-2","volume":"370","author":"RJ Norman","year":"2007","unstructured":"Norman RJ, et al. Polycystic ovary syndrome. Lancet. 2007;370(9588):685\u201397.","journal-title":"Lancet"},{"issue":"1","key":"829_CR4","doi-asserted-by":"publisher","first-page":"41","DOI":"10.1093\/humrep\/deh098","volume":"19","author":"Rotterdam, E.A.-S.P.c.w.g.","year":"2004","unstructured":"Rotterdam, E.A.-S.P.c.w.g. Revised 2003 consensus on diagnostic criteria and long-term health risks related to polycystic ovary syndrome (PCOS). Hum Reprod. 2004;19(1):41\u20137.","journal-title":"Hum Reprod"},{"issue":"6","key":"829_CR5","doi-asserted-by":"publisher","first-page":"2100","DOI":"10.1210\/jc.2005-1494","volume":"91","author":"JM Vink","year":"2006","unstructured":"Vink JM, et al. Heritability of polycystic ovary syndrome in a Dutch twin-family study. J Clin Endocrinol Metab. 2006;91(6):2100\u20134.","journal-title":"J Clin Endocrinol Metab"},{"issue":"1","key":"829_CR6","doi-asserted-by":"publisher","first-page":"69","DOI":"10.1023\/B:REMD.0000016125.05878.96","volume":"5","author":"S Franks","year":"2004","unstructured":"Franks S, McCarthy M. Genetics of ovarian disorders: polycystic ovary syndrome. Rev Endocr Metab Disord. 2004;5(1):69\u201376.","journal-title":"Rev Endocr Metab Disord"},{"issue":"4","key":"829_CR7","doi-asserted-by":"publisher","first-page":"489","DOI":"10.1530\/EJE-14-0252","volume":"171","author":"G Conway","year":"2014","unstructured":"Conway G, et al. European survey of diagnosis and management of the polycystic ovary syndrome: results of the ESE PCOS Special Interest Group\u2019s Questionnaire. Eur J Endocrinol. 2014;171(4):489\u201398.","journal-title":"Eur J Endocrinol"},{"issue":"7","key":"829_CR8","doi-asserted-by":"publisher","first-page":"2093","DOI":"10.1111\/cas.13642","volume":"109","author":"A Sanchez Calle","year":"2018","unstructured":"Sanchez Calle A, et al. Emerging roles of long non-coding RNA in cancer. Cancer Sci. 2018;109(7):2093\u2013100.","journal-title":"Cancer Sci"},{"issue":"3","key":"829_CR9","doi-asserted-by":"publisher","first-page":"177","DOI":"10.1016\/j.gpb.2016.12.005","volume":"15","author":"IM Dykes","year":"2017","unstructured":"Dykes IM, Emanueli C. Transcriptional and post-transcriptional gene regulation by long non-coding RNA. Genomics Proteomics Bioinformatics. 2017;15(3):177\u201386.","journal-title":"Genomics Proteomics Bioinformatics"},{"issue":"4","key":"829_CR10","doi-asserted-by":"publisher","first-page":"BSR20180365","DOI":"10.1042\/BSR20180365","volume":"38","author":"X Huang","year":"2018","unstructured":"Huang X, Zhang W, Shao Z. Association between long non-coding RNA polymorphisms and cancer risk: a meta-analysis. Biosci Rep. 2018;38(4):BSR20180365.","journal-title":"Biosci Rep"},{"issue":"4","key":"829_CR11","doi-asserted-by":"publisher","first-page":"e0152767","DOI":"10.1371\/journal.pone.0152767","volume":"11","author":"F Jiang","year":"2016","unstructured":"Jiang F, Zhou X, Huang J. Long non-coding RNA-ROR mediates the reprogramming in cardiac hypertrophy. PLoS One. 2016;11(4):e0152767.","journal-title":"PLoS One"},{"issue":"1","key":"829_CR12","doi-asserted-by":"publisher","first-page":"69","DOI":"10.1016\/j.devcel.2013.03.002","volume":"25","author":"Y Wang","year":"2013","unstructured":"Wang Y, et al. Endogenous miRNA sponge lincRNA-RoR regulates Oct4, Nanog, and Sox2 in human embryonic stem cell self-renewal. Dev Cell. 2013;25(1):69\u201380.","journal-title":"Dev Cell"},{"key":"829_CR13","doi-asserted-by":"crossref","unstructured":"Shen W, et al. Diagnostic value of lncRNA ROR in differentiating ovarian cancer patients. Clin Lab. 2020;66(7):1261\u20137.","DOI":"10.7754\/Clin.Lab.2019.191035"},{"issue":"8","key":"829_CR14","first-page":"2218","volume":"22","author":"XY Xu","year":"2018","unstructured":"Xu XY, et al. Linc-ROR promotes endometrial cell proliferation by activating the PI3K-Akt pathway. Eur Rev Med Pharmacol Sci. 2018;22(8):2218\u201325.","journal-title":"Eur Rev Med Pharmacol Sci"},{"key":"829_CR15","doi-asserted-by":"publisher","first-page":"2","DOI":"10.1186\/1423-0127-21-2","volume":"21","author":"X Li","year":"2014","unstructured":"Li X, et al. Endometrial progesterone resistance and PCOS. J Biomed Sci. 2014;21:2.","journal-title":"J Biomed Sci"},{"key":"829_CR16","doi-asserted-by":"publisher","first-page":"5011","DOI":"10.12659\/MSM.898883","volume":"22","author":"M Wang","year":"2016","unstructured":"Wang M, et al. MiR-206 Suppresses the progression of coronary artery disease by modulating vascular endothelial growth factor (VEGF) expression. Med Sci Monit. 2016;22:5011\u201320.","journal-title":"Med Sci Monit"},{"issue":"3","key":"829_CR17","doi-asserted-by":"publisher","first-page":"dgz204","DOI":"10.1210\/clinem\/dgz204","volume":"105","author":"M Diaz","year":"2020","unstructured":"Diaz M, et al. Low circulating levels of miR-451a in girls with polycystic ovary syndrome: different effects of randomized treatments. J Clin Endocrinol Metab. 2020;105(3).","journal-title":"J Clin Endocrinol Metab"},{"issue":"8","key":"829_CR18","doi-asserted-by":"publisher","first-page":"3065","DOI":"10.1210\/jc.2009-0542","volume":"94","author":"M Julio-Pieper","year":"2009","unstructured":"Julio-Pieper M, et al. Nerve growth factor induces vascular endothelial growth factor expression in granulosa cells via a trkA receptor\/mitogen-activated protein kinase-extracellularly regulated kinase 2-dependent pathway. J Clin Endocrinol Metab. 2009;94(8):3065\u201371.","journal-title":"J Clin Endocrinol Metab"},{"issue":"3","key":"829_CR19","first-page":"198","volume":"9","author":"Z PourteymourFardTabrizi","year":"2020","unstructured":"PourteymourFardTabrizi Z, et al. Plasma levels of miR-27a, miR-130b, and miR-301a in polycystic ovary syndrome. Int J Mol Cell Med. 2020;9(3):198\u2013206.","journal-title":"Int J Mol Cell Med"},{"issue":"1","key":"829_CR20","doi-asserted-by":"publisher","first-page":"437","DOI":"10.1210\/endo.142.1.7862","volume":"142","author":"Y Nishi","year":"2001","unstructured":"Nishi Y, et al. Establishment and characterization of a steroidogenic human granulosa-like tumor cell line, KGN, that expresses functional follicle-stimulating hormone receptor. Endocrinology. 2001;142(1):437\u201345.","journal-title":"Endocrinology"},{"issue":"41","key":"829_CR21","doi-asserted-by":"publisher","first-page":"69983","DOI":"10.18632\/oncotarget.19545","volume":"8","author":"Y Lou","year":"2017","unstructured":"Lou Y, et al. Linc-ROR induces epithelial-to-mesenchymal transition in ovarian cancer by increasing Wnt\/beta-catenin signaling. Oncotarget. 2017;8(41):69983\u201394.","journal-title":"Oncotarget"},{"issue":"6","key":"829_CR22","doi-asserted-by":"publisher","first-page":"461","DOI":"10.4149\/gpb_2019028","volume":"38","author":"J Li","year":"2019","unstructured":"Li J, et al. Interaction between LncRNA-ROR and miR-145 contributes to epithelial-mesenchymal transition of ovarian cancer cells. Gen Physiol Biophys. 2019;38(6):461\u201371.","journal-title":"Gen Physiol Biophys"},{"issue":"3","key":"829_CR23","doi-asserted-by":"publisher","first-page":"358","DOI":"10.1016\/j.metabol.2003.10.005","volume":"53","author":"M Salehi","year":"2004","unstructured":"Salehi M, et al. Pathogenesis of polycystic ovary syndrome: what is the role of obesity? Metabolism. 2004;53(3):358\u201376.","journal-title":"Metabolism"},{"issue":"9","key":"829_CR24","doi-asserted-by":"publisher","first-page":"1165","DOI":"10.2337\/diab.38.9.1165","volume":"38","author":"A Dunaif","year":"1989","unstructured":"Dunaif A, et al. Profound peripheral insulin resistance, independent of obesity, in polycystic ovary syndrome. Diabetes. 1989;38(9):1165\u201374.","journal-title":"Diabetes"},{"issue":"15","key":"829_CR25","doi-asserted-by":"publisher","first-page":"1863","DOI":"10.2174\/092986709788186093","volume":"16","author":"DA Smiley","year":"2009","unstructured":"Smiley DA, Khalil RA. Estrogenic compounds, estrogen receptors and vascular cell signaling in the aging blood vessels. Curr Med Chem. 2009;16(15):1863\u201387.","journal-title":"Curr Med Chem"},{"issue":"11","key":"829_CR26","doi-asserted-by":"publisher","first-page":"3608","DOI":"10.3390\/ijms19113608","volume":"19","author":"AC Improta Caria","year":"2018","unstructured":"Improta Caria AC, et al. Exercise training-induced changes in microRNAs: beneficial regulatory effects in hypertension, type 2 diabetes, and obesity. Int J Mol Sci. 2018;19(11).","journal-title":"Int J Mol Sci"},{"issue":"4","key":"829_CR27","doi-asserted-by":"publisher","first-page":"816","DOI":"10.1016\/j.jhep.2016.12.016","volume":"66","author":"H Wu","year":"2017","unstructured":"Wu H, et al. MicroRNA-206 prevents hepatosteatosis and hyperglycemia by facilitating insulin signaling and impairing lipogenesis. J Hepatol. 2017;66(4):816\u201324.","journal-title":"J Hepatol"},{"key":"829_CR28","doi-asserted-by":"publisher","first-page":"763","DOI":"10.1016\/j.biopha.2018.05.077","volume":"104","author":"C Dai","year":"2018","unstructured":"Dai C, et al. MiR-206 inhibits epithelial ovarian cancer cells growth and invasion via blocking c-Met\/AKT\/mTOR signaling pathway. Biomed Pharmacother. 2018;104:763\u201370.","journal-title":"Biomed Pharmacother"},{"issue":"4","key":"829_CR29","first-page":"3782","volume":"20","author":"J Shi","year":"2019","unstructured":"Shi J, et al. lncRNA ROR promotes the progression of renal cell carcinoma through the miR206\/VEGF axis. Mol Med Rep. 2019;20(4):3782\u201392.","journal-title":"Mol Med Rep"},{"issue":"5","key":"829_CR30","doi-asserted-by":"publisher","first-page":"978","DOI":"10.1093\/cvr\/cvz021","volume":"115","author":"KB Neves","year":"2019","unstructured":"Neves KB, et al. Microparticles from vascular endothelial growth factor pathway inhibitor-treated cancer patients mediate endothelial cell injury. Cardiovasc Res. 2019;115(5):978\u201388.","journal-title":"Cardiovasc Res"},{"issue":"8","key":"829_CR31","doi-asserted-by":"publisher","first-page":"1992","DOI":"10.1093\/oxfordjournals.humrep.a136222","volume":"10","author":"J Zaidi","year":"1995","unstructured":"Zaidi J, et al. Ovarian stromal blood flow in women with polycystic ovaries\u2013a possible new marker for diagnosis? Hum Reprod. 1995;10(8):1992\u20136.","journal-title":"Hum Reprod"}],"container-title":["Journal of Ovarian Research"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1186\/s13048-021-00829-6.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/article\/10.1186\/s13048-021-00829-6\/fulltext.html","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1186\/s13048-021-00829-6.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,6,21]],"date-time":"2021-06-21T02:03:04Z","timestamp":1624240984000},"score":1,"resource":{"primary":{"URL":"https:\/\/ovarianresearch.biomedcentral.com\/articles\/10.1186\/s13048-021-00829-6"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,6,21]]},"references-count":31,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2021,12]]}},"alternative-id":["829"],"URL":"https:\/\/doi.org\/10.1186\/s13048-021-00829-6","relation":{},"ISSN":["1757-2215"],"issn-type":[{"value":"1757-2215","type":"electronic"}],"subject":[],"published":{"date-parts":[[2021,6,21]]},"assertion":[{"value":"2 February 2021","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"21 May 2021","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"21 June 2021","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Declarations"}},{"value":"This research program follows the ethical principles of human research in the Declaration of Helsinki and has been approved by the Ethics Committee of The First Hospital of Harbin. All recruited individuals in the study have signed informed consent.","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Ethics approval and consent to participate"}},{"value":"All recruited individuals in the study have signed informed consent.","order":3,"name":"Ethics","group":{"name":"EthicsHeading","label":"Consent for publication"}},{"value":"The authors declared that they have no conflict of interests.","order":4,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing interests"}}],"article-number":"79"}}