{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,11,4]],"date-time":"2026-11-04T21:51:16Z","timestamp":1793829076857,"version":"4.6.1"},"reference-count":31,"publisher":"Springer Science and Business Media LLC","issue":"1","license":[{"start":{"date-parts":[[2021,10,22]],"date-time":"2021-10-22T00:00:00Z","timestamp":1634860800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"},{"start":{"date-parts":[[2021,10,22]],"date-time":"2021-10-22T00:00:00Z","timestamp":1634860800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["J Ovarian Res"],"published-print":{"date-parts":[[2021,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec>\n                <jats:title>Background<\/jats:title>\n                <jats:p>Accumulating data have established that microRNAs (miRNAs) play significant regulatory roles in the carcinogenesis and progression of ovarian cancer (OC). <jats:italic>MiR-425-5p<\/jats:italic> was reported to function in various tumors. However, the roles and underlying mechanism of <jats:italic>miR-425-5p<\/jats:italic> involvement in OC development and progression are unclear.<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Methods<\/jats:title>\n                <jats:p>A comprehensive strategy of data mining, computational biology, and real-time polymerase chain reaction was employed to identify the involvement of <jats:italic>miR-425-5p<\/jats:italic> in OC progression. The effect of <jats:italic>miR-425-5p<\/jats:italic> on the proliferation, migration, and invasion of OC cells was determined using Cell Counting Kit-8, wound-healing, and Matrigel invasion assays, respectively. Luciferase assay was performed to evaluate the interactions between <jats:italic>miR-425-5p<\/jats:italic> and <jats:italic>MAGI2-AS3<\/jats:italic> or <jats:italic>AFF4<\/jats:italic>.<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Results<\/jats:title>\n                <jats:p><jats:italic>miR-425-5p<\/jats:italic> was significantly up-regulated in OC tissues and cells. The luciferase reporter assay revealed that <jats:italic>miR-425-5p<\/jats:italic> was negatively regulated by <jats:italic>MAGI2-AS3<\/jats:italic>. Silencing <jats:italic>miR-425-5p<\/jats:italic> inhibited the proliferation, migration, and invasion of OC cells in vitro. Bioinformatics analysis and luciferase reporter assay revealed that <jats:italic>AFF4<\/jats:italic> was the target gene of <jats:italic>miR-425-5p<\/jats:italic>. Moreover, <jats:italic>AFF4<\/jats:italic> expression was significantly decreased in OC and was closely related to the good prognosis of patients with OC. <jats:italic>AFF4<\/jats:italic> overexpression inhibited the proliferation, migration, and invasion of OC cells in vitro. By contrast, silencing <jats:italic>AFF4<\/jats:italic> promoted the proliferation, migration, and invasion of OC cells in vitro. Finally, <jats:italic>AFF4<\/jats:italic> suppression rescued the inhibitory effect of silencing <jats:italic>miR-425-5p<\/jats:italic> on the proliferation, migration, and invasion of OC cells.<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Conclusion<\/jats:title>\n                <jats:p>To the best our knowledge, this is the first study to demonstrate that <jats:italic>miR-425-5p<\/jats:italic> overexpression in OC is negatively regulated by <jats:italic>MAGI2-AS3.<\/jats:italic> Moreover, miR-425-5p promotes the proliferation, migration, and invasion of OC cells by targeting AFF4, suggesting that <jats:italic>miR-425-5p\/AFF4<\/jats:italic> signaling pathway represented a novel therapeutic target for patients with OC.<\/jats:p>\n              <\/jats:sec>","DOI":"10.1186\/s13048-021-00894-x","type":"journal-article","created":{"date-parts":[[2021,10,22]],"date-time":"2021-10-22T14:04:24Z","timestamp":1634911464000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":19,"title":["MiR-425-5p accelerated the proliferation, migration, and invasion of ovarian cancer cells via targeting AFF4"],"prefix":"10.1186","volume":"14","author":[{"given":"Zhihui","family":"Wu","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jianlin","family":"Guo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ying","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5023-6981","authenticated-orcid":false,"given":"Jianhua","family":"Liu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hongping","family":"Ma","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yurong","family":"Tang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2021,10,22]]},"reference":[{"issue":"2","key":"894_CR1","doi-asserted-by":"publisher","first-page":"109","DOI":"10.1111\/j.1601-5037.2006.00177.x","volume":"4","author":"VP Overman","year":"2006","unstructured":"Overman VP. 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