{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,27]],"date-time":"2026-09-27T00:33:29Z","timestamp":1790469209893,"version":"4.1.0"},"reference-count":11,"publisher":"Springer Science and Business Media LLC","issue":"1","license":[{"start":{"date-parts":[[2023,4,30]],"date-time":"2023-04-30T00:00:00Z","timestamp":1682812800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"},{"start":{"date-parts":[[2023,4,30]],"date-time":"2023-04-30T00:00:00Z","timestamp":1682812800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Crit Care"],"abstract":"<jats:title>Abstract<\/jats:title><jats:sec>\n                <jats:title>Background<\/jats:title>\n                <jats:p><jats:italic>Neisseria meningitidis<\/jats:italic> is the leading responsible bacterium of Purpura Fulminans (PF) accounting for two thirds of PF. Skin biopsy is a simple and minimally invasive exam allowing to perform skin culture and polymerase chain reaction (PCR) to detect <jats:italic>Neisseria meningitidis<\/jats:italic>. We aimed to assess the sensitivity of skin biopsy in adult patients with meningococcal PF.<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Methods<\/jats:title>\n                <jats:p>A 17-year multicenter retrospective cohort study including adult patients admitted to the ICU for a meningococcal PF in whom a skin biopsy with conventional and\/or meningococcal PCR was performed.<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Results<\/jats:title>\n                <jats:p>Among 306 patients admitted for PF, 195 had a meningococcal PF (64%) with a skin biopsy being performed in 68 (35%) of them. Skin biopsy was performed in median 1\u00a0day after the initiation of antibiotic therapy. Standard culture of skin biopsy was performed in 61\/68 (90%) patients and grew <jats:italic>Neisseria meningitidis<\/jats:italic> in 28 (46%) of them. <jats:italic>Neisseria meningitidis<\/jats:italic> PCR on skin biopsy was performed in 51\/68 (75%) patients and was positive in 50 (98%) of them. Among these 50 positive meningococcal PCR, five were performed 3\u00a0days or more after initiation of antibiotic therapy. Finally, skin biopsy was considered as contributive in 60\/68 (88%) patients. Identification of the meningococcal serogroup was obtained with skin biopsy in 48\/68 (71%) patients.<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Conclusions<\/jats:title>\n                <jats:p>Skin biopsy with conventional culture and meningococcal PCR has a global sensitivity of 88% and should be systematically considered in case of suspected meningococcal PF even after the initiation of antimicrobial treatment.<\/jats:p>\n              <\/jats:sec>","DOI":"10.1186\/s13054-023-04461-2","type":"journal-article","created":{"date-parts":[[2023,4,30]],"date-time":"2023-04-30T12:01:23Z","timestamp":1682856083000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Skin biopsy in adult patients with meningococcal purpura fulminans: a multicenter retrospective cohort 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