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The main clinical manifestations of ALMS are retinal dystrophy, obesity, type 2 diabetes mellitus, dilated cardiomyopathy and multi-organ fibrosis, characteristic in kidneys and liver. Depletion of the protein encoded by\n                      <jats:italic>ALMS1<\/jats:italic>\n                      has been associated with the alteration of different processes regulated\n                      <jats:italic>via<\/jats:italic>\n                      the primary cilium, such as the NOTCH or TGF-\u03b2 signalling pathways. However, the cellular impact of these deregulated pathways in the absence of ALMS1 remains unknown.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>In this study, we integrated RNA-seq and proteomic analysis to determine the gene expression profile of hTERT-BJ-5ta ALMS1 knockout fibroblasts after TGF-\u03b2 stimulation. In addition, we studied alterations in cross-signalling between the TGF-\u03b2 pathway and the AKT pathway in this cell line.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      We found that ALMS1 depletion affects the TGF-\u03b2 pathway and its cross-signalling with other pathways such as PI3K\/AKT, EGFR1 or p53. In addition, alterations associated with ALMS1 depletion clustered around the processes of extracellular matrix regulation and lipid metabolism in both the transcriptome and proteome. By studying the enriched pathways of common genes differentially expressed in the transcriptome and proteome, collagen fibril organisation, \u03b2-oxidation of fatty acids and eicosanoid metabolism emerged as key processes altered by the absence of ALMS1. Finally, an overactivation of the AKT pathway was determined in the absence of ALMS1 that could be explained by a decrease in\n                      <jats:italic>PTEN<\/jats:italic>\n                      gene expression.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>ALMS1 deficiency disrupts cross-signalling between the TGF-\u03b2 pathway and other dependent pathways in hTERT-BJ-5ta cells. Furthermore, altered cross-signalling impacts the regulation of extracellular matrix-related processes and fatty acid metabolism, and leads to over-activation of the AKT pathway.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1186\/s13062-023-00441-2","type":"journal-article","created":{"date-parts":[[2023,12,7]],"date-time":"2023-12-07T23:23:51Z","timestamp":1701991431000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["Loss of the centrosomal protein ALMS1 alters lipid metabolism and the regulation of extracellular matrix-related processes"],"prefix":"10.1186","volume":"18","author":[{"given":"Brais","family":"Bea-Mascato","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eduardo","family":"G\u00f3mez-Casta\u00f1eda","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yara E.","family":"S\u00e1nchez-Corrales","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sergi","family":"Castellano","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Diana","family":"Valverde","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2023,12,8]]},"reference":[{"key":"441_CR1","doi-asserted-by":"publisher","first-page":"798","DOI":"10.1042\/BST20140070","volume":"42","author":"AG Abraham","year":"2014","unstructured":"Abraham AG, O\u2019Neill E. 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