{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,19]],"date-time":"2026-05-19T03:37:40Z","timestamp":1779161860164,"version":"3.51.4"},"reference-count":65,"publisher":"Springer Science and Business Media LLC","issue":"1","license":[{"start":{"date-parts":[[2024,10,11]],"date-time":"2024-10-11T00:00:00Z","timestamp":1728604800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"},{"start":{"date-parts":[[2024,10,11]],"date-time":"2024-10-11T00:00:00Z","timestamp":1728604800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"}],"funder":[{"DOI":"10.13039\/501100003500","name":"Universit\u00e0 degli Studi di Padova","doi-asserted-by":"crossref","id":[{"id":"10.13039\/501100003500","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Biol Direct"],"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>\n                      miR-210 is one of the most evolutionarily conserved microRNAs. It is known to be involved in several physiological and pathological processes, including response to hypoxia, angiogenesis, cardiovascular diseases and cancer. Recently, new roles of this microRNA are emerging in the context of eye and visual system homeostasis. Recent studies in\n                      <jats:italic>Drosophila melanogaster<\/jats:italic>\n                      unveiled that the absence of miR-210 leads to a progressive retinal degeneration characterized by the accumulation of lipid droplets and disruptions in lipid metabolism. However, the possible conservation of miR-210 knock-out effect in the mammalian retina has yet to be explored.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>We further investigated lipid anabolism and catabolism in miR-210 knock-out (KO) flies, uncovering significant alterations in gene expression within these pathways. Additionally, we characterized the retinal morphology of flies overexpressing (OE) miR-210, which was not affected by the increased levels of the microRNA. For the first time, we also characterized the retinal morphology of miR-210 KO and OE mice. Similar to flies, miR-210 OE did not affect retinal homeostasis, whereas miR-210 KO mice exhibited photoreceptor degeneration. To explore other potential parallels between miR-210 KO models in flies and mice, we examined lipid metabolism, circadian behaviour, and retinal transcriptome in mice, but found no similarities. Specifically, RNA-seq confirmed the lack of involvement of lipid metabolism in the mice\u2019s pathological phenotype, revealing that the differentially expressed genes were predominantly associated with chloride channel activity and extracellular matrix homeostasis. Simultaneously, transcriptome analysis of miR-210 KO fly brains indicated that the observed alterations extend beyond the eye and may be linked to neuronal deficiencies in signal detection and transduction.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>We provide the first morphological characterization of the retina of miR-210 KO and OE mice, investigating the role of this microRNA in mammalian retinal physiology and exploring potential parallels with phenotypes observed in fly models. Although the lack of similarities in lipid metabolism, circadian behaviour, and retinal transcriptome in mice suggests divergent mechanisms of retinal degeneration between the two species, transcriptome analysis of miR-210 KO fly brains indicates the potential existence of a shared upstream mechanism contributing to retinal degeneration in both flies and mammals.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1186\/s13062-024-00542-6","type":"journal-article","created":{"date-parts":[[2024,10,11]],"date-time":"2024-10-11T00:01:38Z","timestamp":1728604898000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["miR-210 is essential to retinal homeostasis in fruit flies and mice"],"prefix":"10.1186","volume":"19","author":[{"given":"Davide","family":"Colaianni","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Federico","family":"Virga","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Annamaria","family":"Tisi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chiara","family":"Stefanelli","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Germana","family":"Zaccagnini","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Paola","family":"Cusumano","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gabriele","family":"Sales","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mihai Bogdan","family":"Preda","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fabio","family":"Martelli","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Daniela","family":"Taverna","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Massimiliano","family":"Mazzone","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Cristiano","family":"Bertolucci","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rita","family":"Maccarone","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Cristiano","family":"De Pitt\u00e0","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2024,10,11]]},"reference":[{"key":"542_CR1","doi-asserted-by":"publisher","unstructured":"He L, Hannon GJ. MicroRNAs: small RNAs with a big role in gene regulation. Nat Rev Genet. 2004; https:\/\/doi.org\/10.1038\/nrg1379.","DOI":"10.1038\/nrg1379"},{"key":"542_CR2","doi-asserted-by":"publisher","unstructured":"Friedman RC, Farh KK, Burge CB, Bartel DP. Most mammalian mRNAs are conserved targets of microRNAs. Genome Res. 2009; https:\/\/doi.org\/10.1101\/gr.082701.108.","DOI":"10.1101\/gr.082701.108"},{"key":"542_CR3","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-62703-748-8_2","author":"KU T\u00fcfekci","year":"2014","unstructured":"T\u00fcfekci KU, Meuwissen RL, Gen\u00e7 S. The role of microRNAs in biological processes. Methods Mol Biol. 2014. https:\/\/doi.org\/10.1007\/978-1-62703-748-8_2.","journal-title":"Methods Mol Biol"},{"key":"542_CR4","doi-asserted-by":"publisher","DOI":"10.1016\/j.gpb.2012.07.005","author":"Y Li","year":"2012","unstructured":"Li Y, Kowdley KV. MicroRNAs in common human diseases. Genomics Proteom Bioinf. 2012. https:\/\/doi.org\/10.1016\/j.gpb.2012.07.005.","journal-title":"Genomics Proteom Bioinf"},{"key":"542_CR5","doi-asserted-by":"publisher","DOI":"10.26508\/lsa.201800149","author":"CM Weigelt","year":"2019","unstructured":"Weigelt CM, Hahn O, Arlt K, Gruhn M, Jahn AJ, E\u00dfer J, Werner JA, Klein C, B\u00fcschges A, Gr\u00f6nke S, Partridge L. Loss of miR-210 leads to progressive retinal degeneration in Drosophila melanogaster. Life Sci Alliance. 2019. https:\/\/doi.org\/10.26508\/lsa.201800149.","journal-title":"Life Sci Alliance"},{"key":"542_CR6","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.01395-06","author":"R Kulshreshtha","year":"2007","unstructured":"Kulshreshtha R, Ferracin M, Wojcik SE, Garzon R, Alder H, Agosto-Perez FJ, Davuluri R, Liu CG, Croce CM, Negrini M, Calin GA, Ivan M. A microRNA signature of hypoxia. Mol Cell Biol. 2007. https:\/\/doi.org\/10.1128\/MCB.01395-06.","journal-title":"Mol Cell Biol"},{"key":"542_CR7","doi-asserted-by":"publisher","DOI":"10.1111\/j.1549-8719.2011.00154.x","author":"YC Chan","year":"2012","unstructured":"Chan YC, Banerjee J, Choi SY, Sen CK. miR-210: the master hypoxamir. Microcirculation. 2012. https:\/\/doi.org\/10.1111\/j.1549-8719.2011.00154.x.","journal-title":"Microcirculation"},{"key":"542_CR8","doi-asserted-by":"publisher","DOI":"10.1002\/iub.427","author":"C Devlin","year":"2011","unstructured":"Devlin C, Greco S, Martelli F, Ivan M. miR-210: more than a silent player in hypoxia. IUBMB Life. 2011. https:\/\/doi.org\/10.1002\/iub.427.","journal-title":"IUBMB Life"},{"key":"542_CR9","doi-asserted-by":"publisher","DOI":"10.1126\/sciadv.abf0466","author":"F Virga","year":"2021","unstructured":"Virga F, Cappellesso F, Stijlemans B, Henze AT, Trotta R, Van Audenaerde J, Mirchandani AS, Sanchez-Garcia MA, Vandewalle J, Orso F, Riera-Domingo C, Griffa A, Ivan C, Smits E, Laoui D, Martelli F, Langouche L, Van den Berghe G, Feron O, Ghesqui\u00e8re B, Prenen H, Libert C, Walmsley SR, Corbet C, Van Ginderachter JA, Ivan M, Taverna D, Mazzone M. Macrophage miR-210 induction and metabolic reprogramming in response to pathogen interaction boost life-threatening inflammation. Sci Adv. 2021. https:\/\/doi.org\/10.1126\/sciadv.abf0466.","journal-title":"Sci Adv"},{"key":"542_CR10","doi-asserted-by":"publisher","DOI":"10.1186\/s12929-020-00658-7","author":"PS Chen","year":"2020","unstructured":"Chen PS, Chiu WT, Hsu PL, Lin SC, Peng IC, Wang CY, Tsai SJ. Pathophysiological implications of hypoxia in human diseases. J Biomed Sci. 2020. https:\/\/doi.org\/10.1186\/s12929-020-00658-7.","journal-title":"J Biomed Sci"},{"key":"542_CR11","doi-asserted-by":"publisher","DOI":"10.1016\/j.prp.2022.154244","author":"S Khalilian","year":"2023","unstructured":"Khalilian S, Bijanvand A, Abedinlou H, Ghafouri-Fard S. A review on the role of miR-210 in human disorders. Pathol Res Pract. 2023. https:\/\/doi.org\/10.1016\/j.prp.2022.154244.","journal-title":"Pathol Res Pract"},{"key":"542_CR12","doi-asserted-by":"publisher","DOI":"10.1089\/ars.2021.0200","author":"G Zaccagnini","year":"2022","unstructured":"Zaccagnini G, Greco S, Voellenkle C, Gaetano C, Martelli F. miR-210 hypoxamiR in Angiogenesis and Diabetes. Antioxid Redox Signal. 2022. https:\/\/doi.org\/10.1089\/ars.2021.0200.","journal-title":"Antioxid Redox Signal"},{"key":"542_CR13","doi-asserted-by":"publisher","DOI":"10.1186\/1756-9966-33-50","author":"Q Qin","year":"2014","unstructured":"Qin Q, Furong W, Baosheng L. Multiple functions of hypoxia-regulated miR-210 in cancer. J Exp Clin Cancer Res. 2014. https:\/\/doi.org\/10.1186\/1756-9966-33-50.","journal-title":"J Exp Clin Cancer Res"},{"key":"542_CR14","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.22.19.6842-6853.2002","author":"S Lavista-Llanos","year":"2002","unstructured":"Lavista-Llanos S, Centanin L, Irisarri M, Russo DM, Gleadle JM, Bocca SN, Muzzopappa M, Ratcliffe PJ, Wappner P. Control of the hypoxic response in Drosophila melanogaster by the basic helix-loop-helix PAS protein similar. Mol Cell Biol. 2002. https:\/\/doi.org\/10.1128\/MCB.22.19.6842-6853.2002.","journal-title":"Mol Cell Biol"},{"key":"542_CR15","doi-asserted-by":"publisher","DOI":"10.1038\/s41418-020-00622-w","author":"J Lyu","year":"2021","unstructured":"Lyu J, Chen Y, Yang W, Guo T, Xu X, Xi Y, Yang X, Ge W. The conserved microRNA miR-210 regulates lipid metabolism and photoreceptor maintenance in the Drosophila retina. Cell Death Differ. 2021. https:\/\/doi.org\/10.1038\/s41418-020-00622-w.","journal-title":"Cell Death Differ"},{"key":"542_CR16","doi-asserted-by":"publisher","DOI":"10.1534\/genetics.118.301583","author":"C Heier","year":"2018","unstructured":"Heier C, K\u00fchnlein RP. Triacylglycerol Metabolism in Drosophila melanogaster. Genetics. 2018. https:\/\/doi.org\/10.1534\/genetics.118.301583.","journal-title":"Genetics"},{"key":"542_CR17","doi-asserted-by":"publisher","DOI":"10.1038\/srep33947","author":"CH Liu","year":"2016","unstructured":"Liu CH, Wang Z, Sun Y, SanGiovanni JP, Chen J. Retinal expression of small non-coding RNAs in a murine model of proliferative retinopathy. Sci Rep. 2016. https:\/\/doi.org\/10.1038\/srep33947.","journal-title":"Sci Rep"},{"key":"542_CR18","doi-asserted-by":"publisher","DOI":"10.1186\/s40001-020-00416-3","author":"C Yin","year":"2020","unstructured":"Yin C, Lin X, Sun Y, Ji X. Dysregulation of miR-210 is involved in the development of diabetic retinopathy and serves a regulatory role in retinal vascular endothelial cell proliferation. Eur J Med Res. 2020. https:\/\/doi.org\/10.1186\/s40001-020-00416-3.","journal-title":"Eur J Med Res"},{"key":"542_CR19","doi-asserted-by":"publisher","DOI":"10.1016\/j.biocel.2009.10.006","author":"P Sapieha","year":"2010","unstructured":"Sapieha P, Hamel D, Shao Z, Rivera JC, Zaniolo K, Joyal JS, Chemtob S. Proliferative retinopathies: angiogenesis that blinds. Int J Biochem Cell Biol. 2010. https:\/\/doi.org\/10.1016\/j.biocel.2009.10.006.","journal-title":"Int J Biochem Cell Biol"},{"key":"542_CR20","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pgen.1007500","author":"P Cusumano","year":"2018","unstructured":"Cusumano P, Biscontin A, Sandrelli F, Mazzotta GM, Tregnago C, De Pitt\u00e0 C, Costa R. Modulation of miR-210 alters phasing of circadian locomotor activity and impairs projections of PDF clock neurons in Drosophila melanogaster. PLoS Genet. 2018. https:\/\/doi.org\/10.1371\/journal.pgen.1007500.","journal-title":"PLoS Genet"},{"key":"542_CR21","doi-asserted-by":"publisher","DOI":"10.1096\/fj.202101423R","author":"N Kaplan","year":"2022","unstructured":"Kaplan N, Liu M, Wang J, Yang W, Fiolek E, Peng H, Lavker RM. Eph signaling is regulated by miRNA-210: implications for corneal epithelial repair. FASEB J. 2022. https:\/\/doi.org\/10.1096\/fj.202101423R.","journal-title":"FASEB J"},{"key":"542_CR22","doi-asserted-by":"publisher","DOI":"10.1016\/j.exer.2022.109350","author":"S Zhao","year":"2023","unstructured":"Zhao S, Fang L, Yan C, Wei J, Song D, Xu C, Luo Y, Fan Y, Guo L, Sun H, Guo T. MicroRNA-210-3p mediates trabecular meshwork extracellular matrix accumulation and ocular hypertension - implication for novel glaucoma therapy. Exp Eye Res. 2023. https:\/\/doi.org\/10.1016\/j.exer.2022.109350.","journal-title":"Exp Eye Res"},{"key":"542_CR23","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2015.04.054","author":"CR Mantel","year":"2015","unstructured":"Mantel CR, O\u2019Leary HA, Chitteti BR, Huang X, Cooper S, Hangoc G, Brustovetsky N, Srour EF, Lee MR, Messina-Graham S, Haas DM, Falah N, Kapur R, Pelus LM, Bardeesy N, Fitamant J, Ivan M, Kim KS, Broxmeyer HE. Enhancing hematopoietic stem cell transplantation efficacy by mitigating oxygen shock. Cell. 2015. https:\/\/doi.org\/10.1016\/j.cell.2015.04.054.","journal-title":"Cell"},{"key":"542_CR24","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pgen.1000857","author":"S Gr\u00f6nke","year":"2010","unstructured":"Gr\u00f6nke S, Clarke DF, Broughton S, Andrews TD, Partridge L. Molecular evolution and functional characterization of Drosophila insulin-like peptides. PLoS Genet. 2010. https:\/\/doi.org\/10.1371\/journal.pgen.1000857.","journal-title":"PLoS Genet"},{"key":"542_CR25","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-017-09763-4","author":"G Zaccagnini","year":"2017","unstructured":"Zaccagnini G, Maimone B, Fuschi P, Maselli D, Spinetti G, Gaetano C, Martelli F. Overexpression of miR-210 and its significance in ischemic tissue damage. Sci Rep. 2017. https:\/\/doi.org\/10.1038\/s41598-017-09763-4.","journal-title":"Sci Rep"},{"key":"542_CR26","doi-asserted-by":"publisher","DOI":"10.1002\/stem.38","author":"ZW Du","year":"2009","unstructured":"Du ZW, Hu BY, Ayala M, Sauer B, Zhang SC. Cre recombination-mediated cassette exchange for building versatile transgenic human embryonic stem cells lines. Stem Cells. 2009. https:\/\/doi.org\/10.1002\/stem.38.","journal-title":"Stem Cells"},{"key":"542_CR27","volume-title":"Animal behaviour in the laboratory: behavioural tests and their interpretation illustrated mainly by psychopharmacology in the rat","author":"P Silverman","year":"1978","unstructured":"Silverman P. Animal behaviour in the laboratory: behavioural tests and their interpretation illustrated mainly by psychopharmacology in the rat. London: Chapman & Hall; 1978."},{"key":"542_CR28","doi-asserted-by":"publisher","DOI":"10.1006\/meth.2001.1262","author":"KJ Livak","year":"2001","unstructured":"Livak KJ, Schmittgen TD. Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) method. Methods. 2001. https:\/\/doi.org\/10.1006\/meth.2001.1262.","journal-title":"Methods"},{"key":"542_CR29","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2105-13-134","author":"J Ye","year":"2012","unstructured":"Ye J, Coulouris G, Zaretskaya I, Cutcutache I, Rozen S, Madden TL. Primer-BLAST: a tool to design target-specific primers for polymerase chain reaction. BMC Bioinformatics. 2012. https:\/\/doi.org\/10.1186\/1471-2105-13-134.","journal-title":"BMC Bioinformatics"},{"key":"542_CR30","doi-asserted-by":"publisher","DOI":"10.1038\/nmeth.4197","author":"R Patro","year":"2017","unstructured":"Patro R, Duggal G, Love MI, Irizarry RA, Kingsford C. Salmon provides fast and bias-aware quantification of transcript expression. Nat Methods. 2017. https:\/\/doi.org\/10.1038\/nmeth.4197.","journal-title":"Nat Methods"},{"key":"542_CR31","doi-asserted-by":"publisher","DOI":"10.12688\/f1000research.7563.2","author":"C Soneson","year":"2015","unstructured":"Soneson C, Love MI, Robinson MD. Differential analyses for RNA-seq: transcript-level estimates improve gene-level inferences. F1000Res. 2015. https:\/\/doi.org\/10.12688\/f1000research.7563.2.","journal-title":"F1000Res"},{"key":"542_CR32","doi-asserted-by":"publisher","unstructured":"Chen Y, Lun AT, Smyth GK. From reads to genes to pathways: differential expression analysis of RNA-Seq experiments using Rsubread and the edgeR quasi-likelihood pipeline. F1000Res. 2016; https:\/\/doi.org\/10.12688\/f1000research.8987.2","DOI":"10.12688\/f1000research.8987.2"},{"key":"542_CR33","doi-asserted-by":"publisher","DOI":"10.1038\/nbt.2931","author":"D Risso","year":"2014","unstructured":"Risso D, Ngai J, Speed TP, Dudoit S. Normalization of RNA-seq data using factor analysis of control genes or samples. Nat Biotechnol. 2014. https:\/\/doi.org\/10.1038\/nbt.2931.","journal-title":"Nat Biotechnol"},{"key":"542_CR34","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btp616","author":"MD Robinson","year":"2010","unstructured":"Robinson MD, McCarthy DJ, Smyth GK. edgeR: a Bioconductor package for differential expression analysis of digital gene expression data. Bioinformatics. 2010. https:\/\/doi.org\/10.1093\/bioinformatics\/btp616.","journal-title":"Bioinformatics"},{"key":"542_CR35","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btz931","author":"SX Ge","year":"2020","unstructured":"Ge SX, Jung D, Yao R. ShinyGO: a graphical gene-set enrichment tool for animals and plants. Bioinformatics. 2020. https:\/\/doi.org\/10.1093\/bioinformatics\/btz931.","journal-title":"Bioinformatics"},{"key":"542_CR36","doi-asserted-by":"publisher","DOI":"10.1177\/0748730411414264","author":"B Schmid","year":"2011","unstructured":"Schmid B, Helfrich-F\u00f6rster C, Yoshii T. A new ImageJ plug-in ActogramJ for chronobiological analyses. J Biol Rhythms. 2011. https:\/\/doi.org\/10.1177\/0748730411414264.","journal-title":"J Biol Rhythms"},{"key":"542_CR37","doi-asserted-by":"publisher","DOI":"10.1002\/dvdy.23707","author":"JP Kumar","year":"2012","unstructured":"Kumar JP. Building an ommatidium one cell at a time. Dev Dyn. 2012. https:\/\/doi.org\/10.1002\/dvdy.23707.","journal-title":"Dev Dyn"},{"key":"542_CR38","doi-asserted-by":"publisher","DOI":"10.1016\/s0074-7696(03)30005-1","author":"GP Lewis","year":"2003","unstructured":"Lewis GP, Fisher SK. Up-regulation of glial fibrillary acidic protein in response to retinal injury: its potential role in glial remodeling and a comparison to vimentin expression. Int Rev Cytol. 2003. https:\/\/doi.org\/10.1016\/s0074-7696(03)30005-1.","journal-title":"Int Rev Cytol"},{"key":"542_CR39","doi-asserted-by":"publisher","DOI":"10.3389\/fncel.2022.804782","author":"L Guo","year":"2022","unstructured":"Guo L, Choi S, Bikkannavar P, Cordeiro MF. Microglia: key players in retinal ageing and neurodegeneration. Front Cell Neurosci. 2022. https:\/\/doi.org\/10.3389\/fncel.2022.804782.","journal-title":"Front Cell Neurosci"},{"key":"542_CR40","doi-asserted-by":"publisher","DOI":"10.1177\/2633105520903708","author":"M Schlichting","year":"2020","unstructured":"Schlichting M. Entrainment of the Drosophila clock by the visual system. Neurosci Insights. 2020. https:\/\/doi.org\/10.1177\/2633105520903708.","journal-title":"Neurosci Insights"},{"key":"542_CR41","doi-asserted-by":"publisher","DOI":"10.1016\/j.brainresrev.2005.08.003","author":"LP Morin","year":"2006","unstructured":"Morin LP, Allen CN. The circadian visual system, 2005. Brain Res Rev. 2006. https:\/\/doi.org\/10.1016\/j.brainresrev.2005.08.003.","journal-title":"Brain Res Rev"},{"key":"542_CR42","doi-asserted-by":"publisher","DOI":"10.3389\/fcell.2022.889677","author":"D Colaianni","year":"2022","unstructured":"Colaianni D, De Pitt\u00e0 C. The role of microRNAs in the Drosophila Melanogaster Visual System. Front Cell Dev Biol. 2022. https:\/\/doi.org\/10.3389\/fcell.2022.889677.","journal-title":"Front Cell Dev Biol"},{"key":"542_CR43","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pgen.1007655","author":"Y Niu","year":"2019","unstructured":"Niu Y, Liu Z, Nian X, Xu X, Zhang Y. miR-210 controls the evening phase of circadian locomotor rhythms through repression of fasciclin 2. PLoS Genet. 2019. https:\/\/doi.org\/10.1371\/journal.pgen.1007655.","journal-title":"PLoS Genet"},{"key":"542_CR44","doi-asserted-by":"publisher","DOI":"10.1007\/s00204-012-0965-5","author":"CC Chio","year":"2013","unstructured":"Chio CC, Lin JW, Cheng HA, Chiu WT, Wang YH, Wang JJ, Hsing CH, Chen RM. MicroRNA-210 targets antiapoptotic Bcl-2 expression and mediates hypoxia-induced apoptosis of neuroblastoma cells. Arch Toxicol. 2013. https:\/\/doi.org\/10.1007\/s00204-012-0965-5.","journal-title":"Arch Toxicol"},{"key":"542_CR45","doi-asserted-by":"publisher","DOI":"10.1016\/j.scr.2013.04.005","author":"F Wang","year":"2013","unstructured":"Wang F, Xiong L, Huang X, Zhao T, Wu LY, Liu ZH, Ding X, Liu S, Wu Y, Zhao Y, Wu K, Zhu LL, Fan M. miR-210 suppresses BNIP3 to protect against the apoptosis of neural progenitor cells. Stem Cell Res. 2013. https:\/\/doi.org\/10.1016\/j.scr.2013.04.005.","journal-title":"Stem Cell Res"},{"key":"542_CR46","doi-asserted-by":"publisher","DOI":"10.1016\/j.stemcr.2016.06.005","author":"AI Abdullah","year":"2016","unstructured":"Abdullah AI, Zhang H, Nie Y, Tang W, Sun T. CDK7 and miR-210 co-regulate cell-cycle progression of neural progenitors in the developing neocortex. Stem Cell Rep. 2016. https:\/\/doi.org\/10.1016\/j.stemcr.2016.06.005.","journal-title":"Stem Cell Rep"},{"key":"542_CR47","doi-asserted-by":"publisher","DOI":"10.1016\/j.neulet.2016.04.034","author":"YW Hu","year":"2016","unstructured":"Hu YW, Jiang JJ, Yan-Gao, Wang RY, Tu GJ. MicroRNA-210 promotes sensory axon regeneration of adult mice in vivo and in vitro. Neurosci Lett. 2016. https:\/\/doi.org\/10.1016\/j.neulet.2016.04.034.","journal-title":"Neurosci Lett"},{"key":"542_CR48","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.1777-16.2017","author":"LA Voloboueva","year":"2017","unstructured":"Voloboueva LA, Sun X, Xu L, Ouyang YB, Giffard RG. Distinct effects of miR-210 reduction on neurogenesis: increased neuronal survival of inflammation but reduced Proliferation Associated with mitochondrial enhancement. J Neurosci. 2017. https:\/\/doi.org\/10.1523\/JNEUROSCI.1777-16.2017.","journal-title":"J Neurosci"},{"key":"542_CR49","doi-asserted-by":"publisher","DOI":"10.1007\/s12035-020-02197-6","author":"M Watts","year":"2021","unstructured":"Watts M, Williams G, Lu J, Nithianantharajah J, Claudianos C. MicroRNA-210 regulates dendritic morphology and behavioural flexibility in mice. Mol Neurobiol. 2021. https:\/\/doi.org\/10.1007\/s12035-020-02197-6.","journal-title":"Mol Neurobiol"},{"key":"542_CR50","doi-asserted-by":"publisher","DOI":"10.3390\/ncrna4020010","author":"ME Watts","year":"2018","unstructured":"Watts ME, Williams SM, Nithianantharajah J, Claudianos C. Hypoxia-Induced MicroRNA-210 targets neurodegenerative pathways. Noncoding RNA. 2018. https:\/\/doi.org\/10.3390\/ncrna4020010.","journal-title":"Noncoding RNA"},{"key":"542_CR51","doi-asserted-by":"publisher","DOI":"10.1515\/revneuro-2013-0017","author":"SA Ojelade","year":"2013","unstructured":"Ojelade SA, Acevedo SF, Rothenfluh A. The role of the actin cytoskeleton in regulating Drosophila behavior. Rev Neurosci. 2013. https:\/\/doi.org\/10.1515\/revneuro-2013-0017.","journal-title":"Rev Neurosci"},{"key":"542_CR52","doi-asserted-by":"publisher","DOI":"10.3390\/cells9092006","author":"M Mikhaylova","year":"2020","unstructured":"Mikhaylova M, Rentsch J, Ewers H. Actomyosin Contractility in the generation and plasticity of axons and dendritic spines. Cells. 2020. https:\/\/doi.org\/10.3390\/cells9092006.","journal-title":"Cells"},{"key":"542_CR53","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M700501200","author":"S Xu","year":"2007","unstructured":"Xu S, Witmer PD, Lumayag S, Kovacs B, Valle D. MicroRNA (miRNA) transcriptome of mouse retina and identification of a sensory organ-specific miRNA cluster. J Biol Chem. 2007. https:\/\/doi.org\/10.1074\/jbc.M700501200.","journal-title":"J Biol Chem"},{"key":"542_CR54","doi-asserted-by":"publisher","DOI":"10.1167\/iovs.09-4657","author":"L Hackler Jr","year":"2010","unstructured":"Hackler L Jr, Wan J, Swaroop A, Qian J, Zack DJ. MicroRNA profile of the developing mouse retina. Invest Ophthalmol Vis Sci. 2010. https:\/\/doi.org\/10.1167\/iovs.09-4657.","journal-title":"Invest Ophthalmol Vis Sci"},{"key":"542_CR55","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkw039","author":"M Karali","year":"2016","unstructured":"Karali M, Persico M, Mutarelli M, Carissimo A, Pizzo M, Singh Marwah V, Ambrosio C, Pinelli M, Carrella D, Ferrari S, Ponzin D, Nigro V, di Bernardo D, Banfi S. High-resolution analysis of the human retina miRNome reveals isomiR variations and novel microRNAs. Nucleic Acids Res. 2016. https:\/\/doi.org\/10.1093\/nar\/gkw039.","journal-title":"Nucleic Acids Res"},{"key":"542_CR56","doi-asserted-by":"publisher","DOI":"10.3390\/ijms24033032","author":"P Carpineto","year":"2023","unstructured":"Carpineto P, Di Filippo ES, Aharrh Gnama A, Bondi D, Iafigliola C, Licata AM, Fulle S. MicroRNA expression in Subretinal Fluid in eyes affected by Rhegmatogenous Retinal detachment. Int J Mol Sci. 2023. https:\/\/doi.org\/10.3390\/ijms24033032.","journal-title":"Int J Mol Sci"},{"key":"542_CR57","first-page":"430","volume":"19","author":"M Ragusa","year":"2013","unstructured":"Ragusa M, Caltabiano R, Russo A, Puzzo L, Avitabile T, Longo A, Toro MD, Di Pietro C, Purrello M, Reibaldi M. MicroRNAs in vitreus humor from patients with ocular diseases. Mol Vis. 2013;19:430\u201340.","journal-title":"Mol Vis"},{"key":"542_CR58","doi-asserted-by":"publisher","DOI":"10.1167\/iovs.19-26663","author":"Y Liu","year":"2019","unstructured":"Liu Y, Wang Y, Chen Y, Fang X, Wen T, Xiao M, Chen S, Zhang X. Discovery and Validation of circulating hsa-mir-210-3p as a potential biomarker for primary Open-Angle Glaucoma. Invest Ophthalmol Vis Sci. 2019. https:\/\/doi.org\/10.1167\/iovs.19-26663.","journal-title":"Invest Ophthalmol Vis Sci"},{"key":"542_CR59","doi-asserted-by":"publisher","DOI":"10.3390\/ijms241914699","author":"M Dobrzycka","year":"2023","unstructured":"Dobrzycka M, Sulewska A, Biecek P, Charkiewicz R, Karabowicz P, Charkiewicz A, Golaszewska K, Milewska P, Michalska-Falkowska A, Nowak K, Niklinski J, Konopi\u0144ska J. miRNA studies in Glaucoma: a Comprehensive Review of current knowledge and future perspectives. Int J Mol Sci. 2023. https:\/\/doi.org\/10.3390\/ijms241914699.","journal-title":"Int J Mol Sci"},{"key":"542_CR60","doi-asserted-by":"publisher","DOI":"10.1152\/physiol.00021.2005","author":"C Hartzell","year":"2005","unstructured":"Hartzell C, Qu Z, Putzier I, Artinian L, Chien LT, Cui Y. Looking chloride channels straight in the eye: bestrophins, lipofuscinosis, and retinal degeneration. Physiol (Bethesda). 2005. https:\/\/doi.org\/10.1152\/physiol.00021.2005.","journal-title":"Physiol (Bethesda)"},{"key":"542_CR61","doi-asserted-by":"publisher","DOI":"10.3390\/cells10030687","author":"AE Pouw","year":"2021","unstructured":"Pouw AE, Greiner MA, Coussa RG, Jiao C, Han IC, Skeie JM, Fingert JH, Mullins RF, Sohn EH. Cell-matrix interactions in the Eye: from Cornea to Choroid. Cells. 2021. https:\/\/doi.org\/10.3390\/cells10030687.","journal-title":"Cells"},{"key":"542_CR62","doi-asserted-by":"publisher","DOI":"10.1167\/iovs.13-13536","author":"MR Al-Ubaidi","year":"2013","unstructured":"Al-Ubaidi MR, Naash MI, Conley SM. A perspective on the role of the extracellular matrix in progressive retinal degenerative disorders. Invest Ophthalmol Vis Sci. 2013. https:\/\/doi.org\/10.1167\/iovs.13-13536.","journal-title":"Invest Ophthalmol Vis Sci"},{"key":"542_CR63","doi-asserted-by":"publisher","DOI":"10.1016\/j.biopha.2017.06.048","author":"C Wang","year":"2017","unstructured":"Wang C, Zhang ZZ, Yang W, Ouyang ZH, Xue JB, Li XL, Zhang J, Chen WK, Yan YG, Wang WJ. MiR-210 facilitates ECM degradation by suppressing autophagy via silencing of ATG7 in human degenerated NP cells. Biomed Pharmacother. 2017. https:\/\/doi.org\/10.1016\/j.biopha.2017.06.048.","journal-title":"Biomed Pharmacother"},{"key":"542_CR64","doi-asserted-by":"publisher","DOI":"10.1038\/ncomms6529","author":"AS Cristino","year":"2014","unstructured":"Cristino AS, Barchuk AR, Freitas FC, Narayanan RK, Biergans SD, Zhao Z, Simoes ZL, Reinhard J, Claudianos C. Neuroligin-associated microRNA-932 targets actin and regulates memory in the honeybee. Nat Commun. 2014. https:\/\/doi.org\/10.1038\/ncomms6529.","journal-title":"Nat Commun"},{"key":"542_CR65","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2583.2010.01010.x","author":"SK Behura","year":"2010","unstructured":"Behura SK, Whitfield CW. Correlated expression patterns of microRNA genes with age-dependent behavioural changes in honeybee. Insect Mol Biol. 2010. https:\/\/doi.org\/10.1111\/j.1365-2583.2010.01010.x.","journal-title":"Insect Mol Biol"}],"container-title":["Biology Direct"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1186\/s13062-024-00542-6.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/article\/10.1186\/s13062-024-00542-6\/fulltext.html","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1186\/s13062-024-00542-6.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,10,11]],"date-time":"2024-10-11T16:24:48Z","timestamp":1728663888000},"score":1,"resource":{"primary":{"URL":"https:\/\/biologydirect.biomedcentral.com\/articles\/10.1186\/s13062-024-00542-6"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,10,11]]},"references-count":65,"journal-issue":{"issue":"1","published-online":{"date-parts":[[2024,12]]}},"alternative-id":["542"],"URL":"https:\/\/doi.org\/10.1186\/s13062-024-00542-6","relation":{"has-preprint":[{"id-type":"doi","id":"10.1101\/2024.01.30.577988","asserted-by":"object"}]},"ISSN":["1745-6150"],"issn-type":[{"value":"1745-6150","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,10,11]]},"assertion":[{"value":"13 September 2024","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"7 October 2024","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"11 October 2024","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Declarations"}},{"value":"For miR-210 KO mice and relative controls, housing and all experimental animal procedures were approved by the Institutional Animal Care and Research Advisory Committee of the KU Leuven (Project number: 085\/2020). For miR-210 OE mice and relative controls, housing and all experimental procedures complied with the Guidelines of the Italian National Institutes of Health and with the Guide for the Care and Use of Laboratory Animals (Institute of Laboratory Animal Resources, National Academy of Sciences, Bethesda, Maryland, USA) and were approved by the institutional Animal Care and Use Committee: Ministero della Salute, Direzione Generale della Sanit\u00e0 Animale e dei Farmaci Veterinari, authorization no. 389\/2020-PR (IACUC 1038).","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Ethics approval and consent to participate"}},{"value":"Not applicable.","order":3,"name":"Ethics","group":{"name":"EthicsHeading","label":"Consent for publication"}},{"value":"The authors declare no competing interests.","order":4,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing interests"}}],"article-number":"90"}}