{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,31]],"date-time":"2026-07-31T18:23:33Z","timestamp":1785522213478,"version":"3.56.0"},"reference-count":56,"publisher":"Springer Science and Business Media LLC","issue":"1","license":[{"start":{"date-parts":[[2024,12,26]],"date-time":"2024-12-26T00:00:00Z","timestamp":1735171200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0"},{"start":{"date-parts":[[2024,12,26]],"date-time":"2024-12-26T00:00:00Z","timestamp":1735171200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["32272854, 32202677, 32202648"],"award-info":[{"award-number":["32272854, 32202677, 32202648"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Shandong Agricultural Elite Variety Project","award":["2022LZGC012, 2021LZG011"],"award-info":[{"award-number":["2022LZGC012, 2021LZG011"]}]},{"DOI":"10.13039\/501100012166","name":"National Key Research and Development Program of China","doi-asserted-by":"publisher","award":["2021YFF1000700"],"award-info":[{"award-number":["2021YFF1000700"]}],"id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Biol Direct"],"DOI":"10.1186\/s13062-024-00579-7","type":"journal-article","created":{"date-parts":[[2024,12,26]],"date-time":"2024-12-26T04:17:31Z","timestamp":1735186651000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Single-cell RNA sequencing reveals the critical role of alternative splicing in cattle testicular spermatagonia"],"prefix":"10.1186","volume":"19","author":[{"given":"Xiuge","family":"Wang","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chunhong","family":"Yang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaochao","family":"Wei","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yaran","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yao","family":"Xiao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jinpeng","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qiang","family":"Jiang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhihua","family":"Ju","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yaping","family":"Gao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yanqin","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yundong","family":"Gao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jinming","family":"Huang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2024,12,26]]},"reference":[{"issue":"11","key":"579_CR1","doi-asserted-by":"publisher","first-page":"1083","DOI":"10.1038\/nmeth.4463","volume":"14","author":"S Aibar","year":"2017","unstructured":"Aibar S, Gonz\u00e1lez-Blas CB, Moerman T, et al. SCENIC: single-cell regulatory network inference and clustering. Nat Methods. 2017;14(11):1083\u201386.","journal-title":"Nat Methods"},{"issue":"1","key":"579_CR2","doi-asserted-by":"publisher","first-page":"423","DOI":"10.1093\/tas\/txz174","volume":"4","author":"ML Butler","year":"2019","unstructured":"Butler ML, Bormann JM, Weaber RL, Grieger DM, Rolf MM. Selection for bull fertility: a review. Transl Anim Sci. 2019;4(1):423\u201341.","journal-title":"Transl Anim Sci"},{"issue":"1","key":"579_CR3","doi-asserted-by":"publisher","first-page":"161","DOI":"10.1016\/j.ydbio.2006.02.027","volume":"294","author":"D Ballow","year":"2006","unstructured":"Ballow D, Meistrich ML, Matzuk M, Rajkovic A. Sohlh1 is essential for spermatogonial differentiation. Dev Biol. 2006;294(1):161\u20137.","journal-title":"Dev Biol"},{"issue":"15","key":"579_CR4","doi-asserted-by":"publisher","first-page":"1970","DOI":"10.3390\/cells12151970","volume":"12","author":"C Cheng","year":"2023","unstructured":"Cheng C, Chen W, Jin H, Chen X. A review of single-cell RNA-Seq annotation, integration, and cell-cell communication. Cells. 2023;12(15):1970.","journal-title":"Cells"},{"issue":"9","key":"579_CR5","doi-asserted-by":"publisher","first-page":"879","DOI":"10.1038\/s41422-018-0074-y","volume":"28","author":"Y Chen","year":"2018","unstructured":"Chen Y, Zheng Y, Gao Y, Lin Z, Yang S, Wang T, Wang Q, Xie N, Hua R, Liu M, Sha J, Griswold MD, Li J, Tang F, Tong MH. Single-cell RNA-seq uncovers dynamic processes and critical regulators in mouse spermatogenesis. Cell Res. 2018;28(9):879\u201396.","journal-title":"Cell Res"},{"issue":"Pt 12","key":"579_CR6","doi-asserted-by":"publisher","first-page":"1933","DOI":"10.1242\/jcs.041236","volume":"122","author":"SR Carmody","year":"2009","unstructured":"Carmody SR, Wente SR. mRNA nuclear export at a glance. J Cell Sci. 2009;122(Pt 12):1933\u20137.","journal-title":"J Cell Sci"},{"key":"579_CR7","doi-asserted-by":"publisher","first-page":"94","DOI":"10.1016\/j.theriogenology.2019.07.008","volume":"138","author":"M Chen","year":"2019","unstructured":"Chen M, Wang J, Liu N, et al. Pig SOX9: expression profiles of sertoli cell (SCs) and a functional 18\u00a0bp indel affecting testis weight. Theriogenology. 2019;138:94\u2013101.","journal-title":"Theriogenology"},{"issue":"5","key":"579_CR8","doi-asserted-by":"publisher","first-page":"P3","DOI":"10.1186\/gb-2003-4-5-p3","volume":"4","author":"G Dennis Jr","year":"2003","unstructured":"Dennis G Jr, Sherman BT, Hosack DA, et al. DAVID: database for annotation, visualization, and Integrated Discovery. Genome Biol. 2003;4(5):P3.","journal-title":"Genome Biol"},{"issue":"5","key":"579_CR9","first-page":"746","volume":"22","author":"DG De Rooij","year":"2001","unstructured":"De Rooij DG, Griswold MD. Questions about spermatogonia posed and answered since 2000. J Androl. 2001;22(5):746\u201357.","journal-title":"J Androl"},{"issue":"2","key":"579_CR10","doi-asserted-by":"publisher","first-page":"394","DOI":"10.1016\/0012-1606(75)90411-X","volume":"44","author":"DW Fawcett","year":"1975","unstructured":"Fawcett DW. The mammalian spermatozoon. Dev Biol. 1975;44(2):394\u2013436.","journal-title":"Dev Biol"},{"issue":"1","key":"579_CR11","doi-asserted-by":"publisher","first-page":"3588","DOI":"10.1038\/s41467-022-31364-7","volume":"13","author":"S Feng","year":"2022","unstructured":"Feng S, Li J, Wen H, Liu K, Gui Y, Wen Y, Wang X, Yuan S. hnRNPH1 recruits PTBP2 and SRSF3 to modulate alternative splicing in germ cells. Nat Commun. 2022;13(1):3588.","journal-title":"Nat Commun"},{"issue":"1","key":"579_CR12","doi-asserted-by":"publisher","first-page":"39","DOI":"10.1186\/s12915-019-0659-z","volume":"17","author":"K Fu","year":"2019","unstructured":"Fu K, Tian S, Tan H, Wang C, Wang H, Wang M, Wang Y, Chen Z, Wang Y, Yue Q, Xu Q, Zhang S, Li H, Xie J, Lin M, Luo M, Chen F, Ye L, Zheng K. Biological and RNA regulatory function of MOV10 in mammalian germ cells. BMC Biol. 2019;17(1):39.","journal-title":"BMC Biol"},{"issue":"12","key":"579_CR13","doi-asserted-by":"publisher","first-page":"1141","DOI":"10.1038\/s41422-018-0099-2","volume":"28","author":"J Guo","year":"2018","unstructured":"Guo J, Grow EJ, Mlcochova H, Maher GJ, Lindskog C, Nie X, Guo Y, Takei Y, Yun J, Cai L, Kim R, Carrell DT, Goriely A, Hotaling JM, Cairns BR. The adult human testis transcriptional cell atlas. Cell Res. 2018;28(12):1141\u201357.","journal-title":"Cell Res"},{"issue":"5","key":"579_CR14","doi-asserted-by":"publisher","first-page":"1144","DOI":"10.1074\/mcp.M112.020123","volume":"12","author":"H Gan","year":"2013","unstructured":"Gan H, Cai T, Lin X, et al. Integrative proteomic and transcriptomic analyses reveal multiple post-transcriptional regulatory mechanisms of mouse spermatogenesis. Mol Cell Proteom. 2013;12(5):1144\u201357.","journal-title":"Mol Cell Proteom"},{"issue":"4","key":"579_CR15","doi-asserted-by":"publisher","first-page":"533","DOI":"10.1016\/j.stem.2017.09.003","volume":"21","author":"J Guo","year":"2017","unstructured":"Guo J, Grow EJ, Yi C, et al. Chromatin and single-cell RNA-Seq profiling reveal Dynamic Signaling and metabolic transitions during human spermatogonial stem Cell Development. Cell Stem Cell. 2017;21(4):533\u201346.","journal-title":"Cell Stem Cell"},{"key":"579_CR16","doi-asserted-by":"publisher","first-page":"651","DOI":"10.1016\/j.devcel.2018.07.025","volume":"46","author":"CD Green","year":"2018","unstructured":"Green CD, Ma Q, Manske GL, Shami AN, Zheng X, Marini S, Moritz L, Sultan C, Gurczynski SJ, Moore BB, et al. A comprehensive roadmap of murine spermatogenesis defined by single-cell RNA-seq. Dev Cell. 2018;46:651\u2013e66710.","journal-title":"Dev Cell"},{"key":"579_CR17","doi-asserted-by":"publisher","first-page":"e1007810","DOI":"10.1371\/journal.pgen.1007810","volume":"15","author":"KJ Grive","year":"2019","unstructured":"Grive KJ, Hu Y, Shu E, Grimson A, Elemento O, Grenier JK, Cohen PE. Dynamic transcriptome profiles within spermatogonial and spermatocyte populations during postnatal testis maturation revealed by single-cell sequencing. PLoS Genet. 2019;15:e1007810.","journal-title":"PLoS Genet"},{"issue":"6","key":"579_CR18","doi-asserted-by":"publisher","first-page":"499","DOI":"10.3727\/000000002783985567","volume":"11","author":"RA Hemendinger","year":"2002","unstructured":"Hemendinger RA, Gores P, Blacksten L, Harley V, Halberstadt C. Identification of a specific sertoli cell marker, Sox9, for use in transplantation. Cell Transpl. 2002;11(6):499\u2013505.","journal-title":"Cell Transpl"},{"key":"579_CR19","doi-asserted-by":"publisher","first-page":"345","DOI":"10.12688\/f1000research.10572.1","volume":"6","author":"KB Hall","year":"2017","unstructured":"Hall KB. RNA and proteins: mutual respect. F1000Res. 2017;6:345.","journal-title":"F1000Res"},{"issue":"3","key":"579_CR20","doi-asserted-by":"publisher","first-page":"e694","DOI":"10.1002\/ctm2.694","volume":"12","author":"D Jovic","year":"2022","unstructured":"Jovic D, Liang X, Zeng H, Lin L, Xu F, Luo Y. Single-cell RNA sequencing technologies and applications: a brief overview. Clin Transl Med. 2022;12(3):e694.","journal-title":"Clin Transl Med"},{"issue":"7","key":"579_CR21","doi-asserted-by":"publisher","first-page":"685","DOI":"10.1016\/j.acthis.2016.08.005","volume":"118","author":"JS Kim","year":"2016","unstructured":"Kim JS, Chae JH, Cheon YP, Kim CG. Reciprocal localization of transcription factors YY1 and CP2c in spermatogonial stem cells and their putative roles during spermatogenesis. Acta Histochem. 2016;118(7):685\u201392.","journal-title":"Acta Histochem"},{"issue":"4","key":"579_CR22","doi-asserted-by":"publisher","first-page":"610","DOI":"10.1016\/j.molcel.2015.04.005","volume":"58","author":"AA Kolodziejczyk","year":"2015","unstructured":"Kolodziejczyk AA, Kim JK, Svensson V, Marioni JC, Teichmann SA. The technology and biology of single-cell RNA sequencing. Mol Cell. 2015;58(4):610\u201320.","journal-title":"Mol Cell"},{"issue":"1","key":"579_CR23","doi-asserted-by":"publisher","first-page":"52","DOI":"10.1093\/biolre\/ioy077","volume":"99","author":"H Kubota","year":"2018","unstructured":"Kubota H, Brinster RL. Spermatogonial stem cells. Biol Reprod. 2018;99(1):52\u201374.","journal-title":"Biol Reprod"},{"issue":"6","key":"579_CR24","doi-asserted-by":"publisher","first-page":"1032","DOI":"10.1111\/rda.12632","volume":"50","author":"JY Kim","year":"2015","unstructured":"Kim JY, Jung HJ, Yoon MJ. VASA (DDX4) is a putative marker for Spermatogonia, Spermatocytes and Round spermatids in stallions. Reprod Domest Anim. 2015;50(6):1032\u20138.","journal-title":"Reprod Domest Anim"},{"issue":"1","key":"579_CR25","doi-asserted-by":"publisher","first-page":"81","DOI":"10.1002\/mrd.10323","volume":"66","author":"J Liu","year":"2003","unstructured":"Liu J, Schiltz JF, Ashar HR, Chada KK. Hmga1 is required for normal sperm development. Mol Reprod Dev. 2003;66(1):81\u20139.","journal-title":"Mol Reprod Dev"},{"key":"579_CR26","doi-asserted-by":"publisher","first-page":"80","DOI":"10.1016\/j.semcdb.2017.10.006","volume":"79","author":"JMD Legrand","year":"2018","unstructured":"Legrand JMD, Hobbs RM. RNA processing in the male germline: mechanisms and implications for fertility. Semin Cell Dev Biol. 2018;79:80\u201391.","journal-title":"Semin Cell Dev Biol"},{"issue":"4","key":"579_CR27","doi-asserted-by":"publisher","first-page":"1267","DOI":"10.1002\/stem.1914","volume":"33","author":"LA Lovasco","year":"2015","unstructured":"Lovasco LA, Gustafson EA, Seymour KA, de Rooij DG, Freiman RN. TAF4b is required for mouse spermatogonial stem cell development. Stem Cells. 2015;33(4):1267\u201376.","journal-title":"Stem Cells"},{"key":"579_CR28","doi-asserted-by":"publisher","first-page":"e78211","DOI":"10.7554\/eLife.78211","volume":"11","author":"W Liu","year":"2022","unstructured":"Liu W, Lu X, Zhao ZH, et al. SRSF10 is essential for progenitor spermatogonia expansion by regulating alternative splicing. Elife. 2022;11:e78211.","journal-title":"Elife"},{"issue":"1","key":"579_CR29","doi-asserted-by":"publisher","first-page":"231","DOI":"10.1186\/s12915-023-01736-6","volume":"21","author":"WL Lei","year":"2023","unstructured":"Lei WL, Du Z, Meng TG, Su R, Li YY, Liu W, Sun SM, Liu MY, Hou Y, Zhang CH, Gui Y, Schatten H, Han Z, Liu C, Sun F, Wang ZB, Qian WP, Sun QY. SRSF2 is required for mRNA splicing during spermatogenesis. BMC Biol. 2023;21(1):231.","journal-title":"BMC Biol"},{"key":"579_CR30","doi-asserted-by":"publisher","first-page":"180192","DOI":"10.1038\/sdata.2018.192","volume":"5","author":"S Lukassen","year":"2018","unstructured":"Lukassen S, Bosch E, Ekici AB, Winterpacht A. Single-cell RNA sequencing of adult mouse testes. Sci Data. 2018;5:180192.","journal-title":"Sci Data"},{"issue":"4","key":"579_CR31","doi-asserted-by":"publisher","first-page":"548","DOI":"10.1016\/j.devcel.2020.07.018","volume":"54","author":"X Lau","year":"2020","unstructured":"Lau X, Munusamy P, Ng MJ, Sangrithi M, Single-Cell RNA. Sequencing of the Cynomolgus Macaque Testis reveals conserved transcriptional profiles during mammalian spermatogenesis. Dev Cell. 2020;54(4):548\u201366.","journal-title":"Dev Cell"},{"issue":"12","key":"579_CR32","doi-asserted-by":"publisher","first-page":"dev200330","DOI":"10.1242\/dev.200330","volume":"149","author":"GE Merges","year":"2022","unstructured":"Merges GE, Meier J, Schneider S, et al. Loss of Prm1 leads to defective chromatin protamination, impaired PRM2 processing, reduced sperm motility and subfertility in male mice. Development. 2022;149(12):dev200330.","journal-title":"Development"},{"issue":"6894","key":"579_CR33","doi-asserted-by":"publisher","first-page":"236","DOI":"10.1038\/418236a","volume":"418","author":"T Maniatis","year":"2002","unstructured":"Maniatis T, Tasic B. Alternative pre-mRNA splicing and proteome expansion in metazoans. Nature. 2002;418(6894):236\u201343.","journal-title":"Nature"},{"issue":"6114","key":"579_CR34","doi-asserted-by":"publisher","first-page":"1593","DOI":"10.1126\/science.1228186","volume":"338","author":"J Merkin","year":"2012","unstructured":"Merkin J, Russell C, Chen P, Burge CB. Evolutionary dynamics of gene and isoform regulation in mammalian tissues. Science. 2012;338(6114):1593\u20139.","journal-title":"Science"},{"issue":"2","key":"579_CR35","doi-asserted-by":"publisher","first-page":"195","DOI":"10.1016\/j.devcel.2007.01.002","volume":"12","author":"T Nakagawa","year":"2007","unstructured":"Nakagawa T, Nabeshima Y, Yoshida S. Functional identification of the actual and potential stem cell compartments in mouse spermatogenesis. Dev Cell. 2007;12(2):195\u2013206.","journal-title":"Dev Cell"},{"issue":"1","key":"579_CR36","doi-asserted-by":"publisher","first-page":"184","DOI":"10.1038\/s41418-022-01057-1","volume":"30","author":"J Qin","year":"2023","unstructured":"Qin J, Huang T, Wang Z, Zhang X, Wang J, Dang Q, Cui D, Wang X, Zhai Y, Zhao L, Lu G, Shao C, Li S, Liu H, Liu Z. Bud31-mediated alternative splicing is required for spermatogonial stem cell self-renewal and differentiation. Cell Death Differ. 2023;30(1):184\u201394.","journal-title":"Cell Death Differ"},{"issue":"3","key":"579_CR37","doi-asserted-by":"publisher","first-page":"309","DOI":"10.1038\/nmeth.4150","volume":"14","author":"X Qiu","year":"2017","unstructured":"Qiu X, Hill A, Packer J, Lin D, Ma YA, Trapnell C. Single-cell mRNA quantification and Differential Analysis with Census. Nat Methods. 2017;14(3):309\u201315.","journal-title":"Nat Methods"},{"issue":"22","key":"579_CR38","doi-asserted-by":"publisher","first-page":"4737","DOI":"10.1016\/j.cell.2023.09.006","volume":"186","author":"N Ripin","year":"2023","unstructured":"Ripin N, Parker R. Formation, function, and pathology of RNP granules. Cell. 2023;186(22):4737\u201356.","journal-title":"Cell"},{"key":"579_CR39","doi-asserted-by":"publisher","first-page":"RP89316","DOI":"10.7554\/eLife.89316","volume":"12","author":"L Sun","year":"2024","unstructured":"Sun L, Lv Z, Chen X, et al. Splicing factor SRSF1 is essential for homing of precursor spermatogonial stem cells in mice. Elife. 2024;12:RP89316.","journal-title":"Elife"},{"issue":"1","key":"579_CR40","doi-asserted-by":"publisher","first-page":"38","DOI":"10.7150\/ijbs.34422","volume":"16","author":"H Song","year":"2020","unstructured":"Song H, Wang L, Chen D, Li F. The function of Pre-mRNA Alternative Splicing in Mammal Spermatogenesis. Int J Biol Sci. 2020;16(1):38\u201348.","journal-title":"Int J Biol Sci"},{"issue":"6","key":"579_CR41","doi-asserted-by":"publisher","first-page":"e0218194","DOI":"10.1371\/journal.pone.0218194","volume":"14","author":"S Sharma","year":"2019","unstructured":"Sharma S, Schlatt S, Van Pelt A, Neuhaus N. Characterization and population dynamics of germ cells in adult macaque testicular cultures. PLoS ONE. 2019;14(6):e0218194.","journal-title":"PLoS ONE"},{"issue":"22","key":"579_CR42","doi-asserted-by":"publisher","first-page":"10170","DOI":"10.1093\/nar\/gkt811","volume":"41","author":"R Schmid","year":"2013","unstructured":"Schmid R, Grellscheid SN, Ehrmann I, et al. The splicing landscape is globally reprogrammed during male meiosis. Nucleic Acids Res. 2013;41(22):10170\u201384.","journal-title":"Nucleic Acids Res"},{"issue":"s1","key":"579_CR43","doi-asserted-by":"publisher","first-page":"s27","DOI":"10.1017\/S1751731118000435","volume":"12","author":"C Staub","year":"2018","unstructured":"Staub C, Johnson L, Review. Spermatogenesis in the bull. Animal. 2018;12(s1):s27\u201335.","journal-title":"Animal"},{"issue":"4","key":"579_CR44","doi-asserted-by":"publisher","first-page":"529","DOI":"10.1016\/j.devcel.2020.05.010","volume":"54","author":"AN Shami","year":"2020","unstructured":"Shami AN, Zheng X, Munyoki SK, et al. Single-cell RNA sequencing of Human, Macaque, and mouse testes uncovers conserved and divergent features of mammalian spermatogenesis. Dev Cell. 2020;54(4):529\u201347.","journal-title":"Dev Cell"},{"key":"579_CR45","doi-asserted-by":"publisher","first-page":"14","DOI":"10.1016\/j.semcdb.2014.02.005","volume":"30","author":"HW Song","year":"2014","unstructured":"Song HW, Wilkinson MF. Transcriptional control of spermatogonial maintenance and differentiation. Semin Cell Dev Biol. 2014;30:14\u201326.","journal-title":"Semin Cell Dev Biol"},{"issue":"10","key":"579_CR46","doi-asserted-by":"publisher","first-page":"1882","DOI":"10.1002\/stem.508","volume":"28","author":"L Simon","year":"2010","unstructured":"Simon L, Ekman GC, Garcia T, et al. ETV5 regulates sertoli cell chemokines involved in mouse stem\/progenitor spermatogonia maintenance. Stem Cells. 2010;28(10):1882\u201392.","journal-title":"Stem Cells"},{"issue":"2","key":"579_CR47","doi-asserted-by":"publisher","first-page":"170","DOI":"10.1111\/jbg.12652","volume":"139","author":"FM Souza","year":"2022","unstructured":"Souza FM, Lopes FB, Rosa GJM, Magnabosco CU. Economic values of reproductive, growth, feed efficiency and carcass traits in Nellore cattle. J Anim Breed Genet. 2022;139(2):170\u201380.","journal-title":"J Anim Breed Genet"},{"issue":"5","key":"579_CR48","doi-asserted-by":"publisher","first-page":"619","DOI":"10.1530\/REP-08-0235","volume":"136","author":"N Vernet","year":"2008","unstructured":"Vernet N, Dennefeld C, Klopfenstein M, et al. Retinoid X receptor beta (RXRB) expression in sertoli cells controls cholesterol homeostasis and spermiation. Reproduction. 2008;136(5):619\u201326.","journal-title":"Reproduction"},{"issue":"9","key":"579_CR49","doi-asserted-by":"publisher","first-page":"7982","DOI":"10.3390\/ijms24097982","volume":"24","author":"X Wang","year":"2023","unstructured":"Wang X, Pei J, Xiong L, et al. Single-cell RNA sequencing reveals Atlas of Yak Testis cells. Int J Mol Sci. 2023;24(9):7982.","journal-title":"Int J Mol Sci"},{"issue":"4","key":"579_CR50","doi-asserted-by":"publisher","first-page":"701","DOI":"10.1016\/j.cell.2009.02.009","volume":"136","author":"MC Wahl","year":"2009","unstructured":"Wahl MC, Will CL, L\u00fchrmann R. The spliceosome: design principles of a dynamic RNP machine. Cell. 2009;136(4):701\u201318.","journal-title":"Cell"},{"issue":"2","key":"579_CR51","doi-asserted-by":"publisher","first-page":"113769","DOI":"10.1016\/j.celrep.2024.113769","volume":"43","author":"X Wang","year":"2024","unstructured":"Wang X, Fan X, Zhang J, et al. hnRNPA2B1 represses the disassembly of arsenite-induced stress granules and is essential for male fertility. Cell Rep. 2024;43(2):113769.","journal-title":"Cell Rep"},{"issue":"23","key":"579_CR52","doi-asserted-by":"publisher","first-page":"6245","DOI":"10.1128\/MCB.00679-09","volume":"29","author":"S Wu","year":"2009","unstructured":"Wu S, Hu YC, Liu H, Shi Y. Loss of YY1 impacts the heterochromatic state and meiotic double-strand breaks during mouse spermatogenesis. Mol Cell Biol. 2009;29(23):6245\u201356.","journal-title":"Mol Cell Biol"},{"issue":"0","key":"579_CR53","doi-asserted-by":"publisher","first-page":"53","DOI":"10.1016\/S0074-7696(08)61357-1","volume":"37","author":"G Yasuzumi","year":"1974","unstructured":"Yasuzumi G. Electron microscope studies on spermiogenesis in various animal species. Int Rev Cytol. 1974;37(0):53\u2013119.","journal-title":"Int Rev Cytol"},{"issue":"4","key":"579_CR54","doi-asserted-by":"publisher","first-page":"401","DOI":"10.24272\/j.issn.2095-8137.2020.373","volume":"42","author":"XW Yu","year":"2021","unstructured":"Yu XW, Li TT, Du XM, et al. Single-cell RNA sequencing reveals atlas of dairy goat testis cells. Zool Res. 2021;42(4):401\u20135.","journal-title":"Zool Res"},{"issue":"1","key":"579_CR55","doi-asserted-by":"publisher","first-page":"122","DOI":"10.1186\/s40104-021-00638-3","volume":"12","author":"L Zhang","year":"2021","unstructured":"Zhang L, Li F, Lei P, et al. Single-cell RNA-sequencing reveals the dynamic process and novel markers in porcine spermatogenesis. J Anim Sci Biotechnol. 2021;12(1):122.","journal-title":"J Anim Sci Biotechnol"},{"issue":"8","key":"579_CR56","doi-asserted-by":"publisher","first-page":"1859","DOI":"10.1016\/j.stemcr.2022.06.010","volume":"17","author":"S Zhou","year":"2022","unstructured":"Zhou S, Dong J, Xiong M, et al. UHRF1 interacts with snRNAs and regulates alternative splicing in mouse spermatogonial stem cells. Stem Cell Rep. 2022;17(8):1859\u201373.","journal-title":"Stem Cell Rep"}],"container-title":["Biology Direct"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1186\/s13062-024-00579-7.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/article\/10.1186\/s13062-024-00579-7\/fulltext.html","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1186\/s13062-024-00579-7.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,12,26]],"date-time":"2024-12-26T06:06:03Z","timestamp":1735193163000},"score":1,"resource":{"primary":{"URL":"https:\/\/biologydirect.biomedcentral.com\/articles\/10.1186\/s13062-024-00579-7"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,12,26]]},"references-count":56,"journal-issue":{"issue":"1","published-online":{"date-parts":[[2024,12]]}},"alternative-id":["579"],"URL":"https:\/\/doi.org\/10.1186\/s13062-024-00579-7","relation":{},"ISSN":["1745-6150"],"issn-type":[{"value":"1745-6150","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,12,26]]},"assertion":[{"value":"31 July 2024","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"5 December 2024","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"26 December 2024","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Declarations"}},{"value":"All experimental procedures involving animals were ethically approved by the Institutional Animal Care and Use Committee of the Institute of Animal Science and Veterinary Medicine, Shandong Academy of Agricultural Sciences (IACC20060101).","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Ethics approval and consent to participate"}},{"value":"Not applicable.","order":3,"name":"Ethics","group":{"name":"EthicsHeading","label":"Consent for publication"}},{"value":"The authors declare no competing interests.","order":4,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing interests"}}],"article-number":"145"}}