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The drugs are used co-administered for the treatment of Leukemia, a certain type of blood cancer. Method (A) is an isocratic chromatographic HPLC method; separation was accomplished on C18 column using the eluting mixture of 6.9 g\/L Monobasic Sodium Phosphate pH 3: methanol (70:30, v\/v) and detection was at 275 nm. Concentrations were in the range of 0.2\u201315 \u03bcg\/mL for both CYT and DEX. Method (B) is a HPTLC method in which separation was attained on HPTLC F254 plates using methanol: ethyl acetate: ammonia, (7.8:2:0.2, by volume) as eluting solvents and detection was at 275 nm. Concentrations were in the range of 0.1\u20134 \u03bcg\/band for both CYT and DEX. The parameters for system suitability testing were evaluated to determine the effectiveness of the developed chromatographic procedures in terms of performance. The recently developed techniques were applied for the determination of the drugs under investigation in spiked human plasma. Validation parameters were examined in accordance with US-FDA criteria. All results were found to be within the acceptable ranges. To evaluate the greenness characters of the proposed methods to the environment; three greenness assessment tools including eco-scale assessments (ESA), green analytical procedure index (GAPI), and Analytical Greenness calculator (AGREE) were used. Acceptable and satisfying results that demonstrated the greenness characteristics of the suggested methods were attained.<\/jats:p>","DOI":"10.1186\/s13065-024-01184-5","type":"journal-article","created":{"date-parts":[[2024,5,3]],"date-time":"2024-05-03T03:37:24Z","timestamp":1714707444000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Two chromatographic methods for determination of cytarabine and dexamethazone; two co-administered drugs for the treatment of leukemia: green analytical perspective"],"prefix":"10.1186","volume":"18","author":[{"given":"Maimana A.","family":"Magdy","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ahmed M.","family":"Elgebaly","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ahmed A.","family":"Taha","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nourddin W.","family":"Ali","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nehal F.","family":"Farid","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2024,5,3]]},"reference":[{"key":"1184_CR1","unstructured":"https:\/\/www.cancer.org\/cancer\/acutemyeloidleukemia\/treating\/chemotherapy.Html. 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Differential Analysis of Cyclocytidine, 1-\u03b2-D-Arabinofuranosylcytosine, and 1-\u03b2-D-Arabinofuranosyluracil by Ultraviolet Spectophotometry. J Nat Cancer Inst. 1974;52:1521\u20138. https:\/\/doi.org\/10.1093\/jnci.52.51521.","journal-title":"J Nat Cancer Inst"},{"key":"1184_CR6","doi-asserted-by":"publisher","first-page":"573","DOI":"10.1002\/rcm.2877","volume":"21","author":"Y Hsieh","year":"2007","unstructured":"Hsieh Y, Duncan CJ. An ion pairing liquid chromatography\/tandem mass spectrometric method for the determination of cytarabine in mouse plasma. Rapid Commun Mass Spectrom. 2007;21:573\u20138. https:\/\/doi.org\/10.1002\/rcm.2877.","journal-title":"Rapid Commun Mass Spectrom"},{"key":"1184_CR7","doi-asserted-by":"publisher","first-page":"8","DOI":"10.1016\/j.jchromb.2007.03.034","volume":"854","author":"Y Hsieh","year":"2007","unstructured":"Hsieh Y, Duncan CJ, Liu M. A mixed-mode liquid chromatography-tandem mass spectrometric method for the determination of cytarabine in mouse plasma. J Chromatogr B. 2007;854:8\u201312. https:\/\/doi.org\/10.1016\/j.jchromb.2007.03.034.","journal-title":"J Chromatogr B"},{"key":"1184_CR8","doi-asserted-by":"publisher","first-page":"1603","DOI":"10.4155\/bio.11.140","volume":"3","author":"MJ Hilhorst","year":"2011","unstructured":"Hilhorst MJ, Hendriks G, Van Hout MW, Sillen H, Van de Merbel NC. HPLC\u2013MS\/MS method for the determination of cytarabine in human plasma. Bioanalysis. 2011;3:1603\u201311. https:\/\/doi.org\/10.4155\/bio.11.140.","journal-title":"Bioanalysis"},{"key":"1184_CR9","doi-asserted-by":"publisher","first-page":"14","DOI":"10.1016\/j.jchromb.20014.05.0034","volume":"962","author":"D Liang","year":"2014","unstructured":"Liang D, Wang W, Jiang X, Yin S. 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The reference number (Serial No.: REC-H-PhBSU-24007).The informed consent to participate was obtained from all of the participants in the study.","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Ethics approval and consent to participate"}},{"value":"Not applicable.","order":3,"name":"Ethics","group":{"name":"EthicsHeading","label":"Consent for publication"}},{"value":"There are no conflicts to declare.","order":4,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing interests"}}],"article-number":"88"}}