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The HPTLC analysis utilized a mobile phase consisting of toluene: ethyl acetate: methanol: acetone: acetic acid (6:1.5:1:0.5:1, v\/v\/v\/v\/v) on an aluminum-backed layer of silica gel 60 F254. Amlodipine demonstrated a linear relationship within the range of 60\u2013600 ng\/band, while the AIIRA drugs showed linearity within the range of 90\u2013900 ng\/band. The proposed method exhibited good linearity, with correlation coefficients (r) ranging from 0.9939 to 0.9998 for all five studied mixtures. The method was validated according to International Council for Harmonization guidelines for linearity, accuracy, precision, robustness, and detection and quantitation limits. Amlodipine and the investigated Angiotensin II Receptor Antagonist drugs were successfully detected and quantified in both bulk drug and plasma samples, yielding high recovery percentages and low standard deviation values. The greenness of the proposed HPTLC method has been evaluated using the standards of greenness profile (AGREE and GAPI) and Eco-Scale.<\/jats:p>","DOI":"10.1186\/s13065-026-01765-6","type":"journal-article","created":{"date-parts":[[2026,3,23]],"date-time":"2026-03-23T20:42:59Z","timestamp":1774298579000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["High-performance thin-layer chromatographic method for simultaneous determination of some Angiotensin II Receptor Antagonists with amlodipine in spiked human plasma with UV detection"],"prefix":"10.1186","volume":"20","author":[{"given":"Ahmed A.","family":"Khorshed","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fatma M.","family":"Abdelnaeem","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dalia M.","family":"Nagy","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mohamed","family":"Oraby","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sayed M.","family":"Derayea","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2026,3,23]]},"reference":[{"issue":"9369","key":"1765_CR1","doi-asserted-by":"publisher","first-page":"1629","DOI":"10.1016\/S0140-6736(03)13302-8","volume":"361","author":"JA Staessen","year":"2003","unstructured":"Staessen JA, Wang J, Bianchi G, Birkenh\u00e4ger WH. 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J Appl Spectrosc. 2011;77:869\u201377.","journal-title":"J Appl Spectrosc"}],"container-title":["BMC Chemistry"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/article\/10.1186\/s13065-026-01765-6","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1186\/s13065-026-01765-6.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1186\/s13065-026-01765-6.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,4,9]],"date-time":"2026-04-09T09:01:59Z","timestamp":1775725319000},"score":1,"resource":{"primary":{"URL":"https:\/\/link.springer.com\/10.1186\/s13065-026-01765-6"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,3,23]]},"references-count":53,"journal-issue":{"issue":"1","published-online":{"date-parts":[[2026,12]]}},"alternative-id":["1765"],"URL":"https:\/\/doi.org\/10.1186\/s13065-026-01765-6","relation":{"has-preprint":[{"id-type":"doi","id":"10.21203\/rs.3.rs-4938215\/v1","asserted-by":"object"}]},"ISSN":["2661-801X"],"issn-type":[{"value":"2661-801X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,3,23]]},"assertion":[{"value":"19 August 2024","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"19 February 2026","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"23 March 2026","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Declarations"}},{"value":"This work was approved by the Committee of Research Ethics in the Faculty of Pharmacy, Minia University, Minia, Egypt with approval number (MPEC (250804)). All participants signed informed consent statements before participation in the study. Procedures involving human plasma were performed in accordance with relevant guidelines and regulations, and in compliance with the Declaration of Helsinki.","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Ethics approval and consent to participate"}},{"value":"Not applicable.","order":3,"name":"Ethics","group":{"name":"EthicsHeading","label":"Consent for publication"}},{"value":"The authors declare no competing interests.","order":4,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing interests"}}],"article-number":"79"}}