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Single-carbon feedstocks, derived from CO\n                      <jats:sub>2<\/jats:sub>\n                      , represent promising substrates for biotechnological applications. Especially, methanol is gaining prominence for bio-production of commodity chemicals.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      In this study, we show the potential of\n                      <jats:italic>Komagataella phaffii<\/jats:italic>\n                      as a production platform for itaconic acid using methanol as the carbon source. Successful integration of heterologous genes from\n                      <jats:italic>Aspergillus terreus<\/jats:italic>\n                      (\n                      <jats:italic>cadA<\/jats:italic>\n                      ,\n                      <jats:italic>mttA<\/jats:italic>\n                      and\n                      <jats:italic>mfsA<\/jats:italic>\n                      ) alongside fine-tuning of the\n                      <jats:italic>mfsA<\/jats:italic>\n                      gene expression, led to promising initial itaconic acid titers of 28\u00a0g\u00b7L\n                      <jats:sup>\u22121<\/jats:sup>\n                      after 5 days of fed-batch cultivation. Through the combined efforts of process optimization and strain engineering strategies, we further boosted the itaconic acid production reaching titers of 55\u00a0g\u00b7L\n                      <jats:sup>\u22121<\/jats:sup>\n                      after less than 5 days of methanol feed, while increasing the product yield on methanol from 0.06\u00a0g\u00b7g\n                      <jats:sup>\u22121<\/jats:sup>\n                      to 0.24\u00a0g\u00b7g\n                      <jats:sup>\u22121<\/jats:sup>\n                      .\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>\n                      Our results highlight the potential of\n                      <jats:italic>K. phaffii<\/jats:italic>\n                      as a methanol-based platform organism for sustainable biochemical production.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1186\/s13068-024-02541-1","type":"journal-article","created":{"date-parts":[[2024,7,15]],"date-time":"2024-07-15T13:01:59Z","timestamp":1721048519000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":25,"title":["Efficient production of itaconic acid from the single-carbon\u00a0substrate methanol with engineered Komagataella phaffii"],"prefix":"10.1186","volume":"17","author":[{"given":"Manja M\u00f8lgaard","family":"Severinsen","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Simone","family":"Bachleitner","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Viola","family":"Modenese","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"\u00d6zge","family":"Ata","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Diethard","family":"Mattanovich","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2024,7,15]]},"reference":[{"key":"2541_CR1","doi-asserted-by":"publisher","first-page":"5301","DOI":"10.5194\/essd-15-5301-2023","volume":"15","author":"P Friedlingstein","year":"2023","unstructured":"Friedlingstein P, O\u2019Sullivan M, Jones MW, Andrew RM, Bakker DCE, Hauck J, et al. 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