{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,24]],"date-time":"2026-10-24T00:29:56Z","timestamp":1792801796541,"version":"4.5.0"},"reference-count":41,"publisher":"Springer Science and Business Media LLC","issue":"1","funder":[{"name":"UNICEF\/UNDP\/World bank\/WHO Special Programme for Research and Training in Tropical Diseases (TDR-WHO)","award":["201293485"],"award-info":[{"award-number":["201293485"]}],"id":[{"id":"https:\/\/ror.org\/046j7pv84","id-type":"ROR","asserted-by":"crossref"}]}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Parasites Vectors"],"published-print":{"date-parts":[[2016,12]]},"DOI":"10.1186\/s13071-016-1572-8","type":"journal-article","created":{"date-parts":[[2016,5,13]],"date-time":"2016-05-13T13:13:37Z","timestamp":1463145217000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":111,"title":["Mobile suitcase laboratory for rapid detection of Leishmania donovani using recombinase polymerase amplification assay"],"prefix":"10.1186","volume":"9","author":[{"given":"Dinesh","family":"Mondal","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Prakash","family":"Ghosh","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Md Anik Ashfaq","family":"Khan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Faria","family":"Hossain","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Susanne","family":"B\u00f6hlken-Fascher","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Greg","family":"Matlashewski","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Axel","family":"Kroeger","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Piero","family":"Olliaro","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ahmed","family":"Abd El Wahed","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2016,5,13]]},"reference":[{"issue":"1","key":"1572_CR1","doi-asserted-by":"crossref","first-page":"e355","DOI":"10.1371\/journal.pntd.0000355","volume":"3","author":"D Mondal","year":"2009","unstructured":"Mondal D, Singh SP, Kumar N, Joshi A, Sundar S, Das P, Siddhivinayak H, Kroeger A, Boelaert M. Visceral leishmaniasis elimination programme in India, Bangladesh, and Nepal: reshaping the case finding\/case management strategy. PLoS Negl Trop Dis. 2009;3(1):e355.","journal-title":"PLoS Negl Trop Dis"},{"issue":"11","key":"1572_CR2","doi-asserted-by":"crossref","first-page":"873","DOI":"10.1038\/nrmicro1748","volume":"5","author":"F Chappuis","year":"2007","unstructured":"Chappuis F, Sundar S, Hailu A, Ghalib H, Rijal S, Peeling RW, Alvar J, Boelaert M. Visceral leishmaniasis: what are the needs for diagnosis, treatment and control? Nat Rev Microbiol. 2007;5(11):873\u201382.","journal-title":"Nat Rev Microbiol"},{"issue":"5","key":"1572_CR3","doi-asserted-by":"crossref","first-page":"e0003738","DOI":"10.1371\/journal.pntd.0003738","volume":"9","author":"E Diro","year":"2015","unstructured":"Diro E, Lynen L, Assefa M, Takele Y, Mengesha B, Adem E, Mohammed R, Kimutai R, Hailu A, Boelaert M, et al. Impact of the use of a rapid diagnostic test for visceral leishmaniasis on clinical practice in Ethiopia: a retrospective study. PLoS Negl Trop Dis. 2015;9(5):e0003738.","journal-title":"PLoS Negl Trop Dis"},{"issue":"3\u20134","key":"1572_CR4","first-page":"294","volume":"12","author":"A Alborzi","year":"2006","unstructured":"Alborzi A, Rasouli M, Nademi Z, Kadivar MR, Pourabbas B. Evaluation of rK39 strip test for the diagnosis of visceral leishmaniasis in infants. East Mediterr Health J. 2006;12(3\u20134):294\u20139.","journal-title":"East Mediterr Health J"},{"issue":"8","key":"1572_CR5","doi-asserted-by":"crossref","first-page":"961","DOI":"10.3855\/jidc.4310","volume":"8","author":"EK Elmahallawy","year":"2014","unstructured":"Elmahallawy EK, Sampedro Martinez A, Rodriguez-Granger J, Hoyos-Mallecot Y, Agil A, Navarro Mari JM, Gutierrez Fernandez J. Diagnosis of leishmaniasis. J Infect Dev Ctries. 2014;8(8):961\u201372.","journal-title":"J Infect Dev Ctries"},{"key":"1572_CR6","unstructured":"WHO. Visceral Leishmaniasis rapid diagnostic test performance. Diagn Eval Ser. 2011;4. http:\/\/www.who.int\/tdr\/publications\/documents\/vl-rdt-evaluation.pdf?ua=1 ."},{"issue":"1","key":"1572_CR7","doi-asserted-by":"crossref","first-page":"e2657","DOI":"10.1371\/journal.pntd.0002657","volume":"8","author":"E Hasker","year":"2014","unstructured":"Hasker E, Malaviya P, Gidwani K, Picado A, Ostyn B, Kansal S, Singh RP, Singh OP, Chourasia A, Kumar Singh A, et al. Strong association between serological status and probability of progression to clinical visceral leishmaniasis in prospective cohort studies in India and Nepal. PLoS Negl Trop Dis. 2014;8(1):e2657.","journal-title":"PLoS Negl Trop Dis"},{"key":"1572_CR8","doi-asserted-by":"crossref","unstructured":"Nicolas L, Milon G, Prina E. Rapid differentiation of Old World Leishmania species by LightCycler polymerase chain reaction and melting curve analysis. J Microbiol Methods. 2002;51(3):295\u20139.","DOI":"10.1016\/S0167-7012(02)00099-4"},{"key":"1572_CR9","doi-asserted-by":"crossref","unstructured":"Srivastava A, Sweat JM, Azizan A, Vesely B, Kyle DE. Real-time PCR to quantify Leishmania donovani in hamsters. J Parasitol. 2013;99(1):145\u201350.","DOI":"10.1645\/GE-3221.1"},{"key":"1572_CR10","doi-asserted-by":"crossref","unstructured":"Stevenson LG, Fedorko DP, Zelazny AM. An enhanced method for the identification of Leishmania spp. using real-time polymerase chain reaction and sequence analysis of the 7SL RNA gene region. Diagn Microbiol Infect Dis. 2010;66(4):432\u20135.","DOI":"10.1016\/j.diagmicrobio.2009.11.005"},{"key":"1572_CR11","doi-asserted-by":"crossref","unstructured":"Tupperwar N, Vineeth V, Rath S, Vaidya T. Development of a real-time polymerase chain reaction assay for the quantification of Leishmania species and the monitoring of systemic distribution of the pathogen. Diagn Microbiol Infect Dis. 2008;61(1):23\u201330.","DOI":"10.1016\/j.diagmicrobio.2007.12.013"},{"key":"1572_CR12","doi-asserted-by":"crossref","unstructured":"Wortmann G, Hochberg L, Houng HH, Sweeney C, Zapor M, Aronson N, Weina P, Ockenhouse CF. Rapid identification of Leishmania complexes by a real-time PCR assay. Am J Trop Med Hyg. 2005;73(6):999\u20131004.","DOI":"10.4269\/ajtmh.2005.73.999"},{"issue":"6","key":"1572_CR13","doi-asserted-by":"crossref","first-page":"e0129682","DOI":"10.1371\/journal.pone.0129682","volume":"10","author":"A Abd El Wahed","year":"2015","unstructured":"Abd El Wahed A, Patel P, Faye O, Thaloengsok S, Heidenreich D, Matangkasombut P, Manopwisedjaroen K, Sakuntabhai A, Sall AA, Hufert FT, et al. Recombinase polymerase amplification assay for rapid diagnostics of dengue infection. PLoS One. 2015;10(6):e0129682.","journal-title":"PLoS One"},{"issue":"7580","key":"1572_CR14","doi-asserted-by":"crossref","first-page":"S102","DOI":"10.1038\/nature16042","volume":"528","author":"GF Medley","year":"2015","unstructured":"Medley GF, Hollingsworth TD, Olliaro PL, Adams ER. Health-seeking behaviour, diagnostics and transmission dynamics in the control of visceral leishmaniasis in the Indian subcontinent. Nature. 2015;528(7580):S102\u2013108.","journal-title":"Nature"},{"issue":"7","key":"1572_CR15","doi-asserted-by":"crossref","first-page":"e204","DOI":"10.1371\/journal.pbio.0040204","volume":"4","author":"O Piepenburg","year":"2006","unstructured":"Piepenburg O, Williams CH, Stemple DL, Armes NA. DNA detection using recombination proteins. PLoS Biol. 2006;4(7):e204.","journal-title":"PLoS Biol"},{"issue":"11","key":"1572_CR16","doi-asserted-by":"crossref","first-page":"e112146","DOI":"10.1371\/journal.pone.0112146","volume":"9","author":"ZA Crannell","year":"2014","unstructured":"Crannell ZA, Rohrman B, Richards-Kortum R. Equipment-free incubation of recombinase polymerase amplification reactions using body heat. PLoS One. 2014;9(11):e112146.","journal-title":"PLoS One"},{"key":"1572_CR17","doi-asserted-by":"crossref","unstructured":"Abd El Wahed A, Patel P, Heidenreich D, Hufert FT, Weidmann M. Reverse transcription recombinase polymerase amplification assay for the detection of middle East respiratory syndrome coronavirus. PLoS Curr. 2013;5.","DOI":"10.1371\/currents.outbreaks.62df1c7c75ffc96cd59034531e2e8364"},{"key":"1572_CR18","doi-asserted-by":"crossref","first-page":"16","DOI":"10.1016\/j.jcv.2015.05.004","volume":"69","author":"A Abd El Wahed","year":"2015","unstructured":"Abd El Wahed A, Weidmann M, Hufert FT. Diagnostics-in-a-Suitcase: Development of a portable and rapid assay for the detection of the emerging avian influenza A (H7N9) virus. J Clin Virol. 2015;69:16\u201321.","journal-title":"J Clin Virol"},{"key":"1572_CR19","doi-asserted-by":"crossref","unstructured":"Faye O, Faye O, Soropogui B, Patel P, El Wahed AA, Loucoubar C, Fall G, Kiory D, Magassouba N, Keita S, et al. Development and deployment of a rapid recombinase polymerase amplification Ebola virus detection assay in Guinea in 2015. Euro Surveill. 2015;20(44). doi: 10.2807\/1560-7917.ES.2015.20.44.30053","DOI":"10.2807\/1560-7917.ES.2015.20.44.30053"},{"issue":"1","key":"1572_CR20","doi-asserted-by":"crossref","first-page":"e16025","DOI":"10.1371\/journal.pone.0016025","volume":"6","author":"C Zhong","year":"2011","unstructured":"Zhong C, Peng D, Ye W, Chai L, Qi J, Yu Z, Ruan L, Sun M. Determination of plasmid copy number reveals the total plasmid DNA amount is greater than the chromosomal DNA amount in Bacillus thuringiensis YBT-1520. PLoS One. 2011;6(1):e16025.","journal-title":"PLoS One"},{"issue":"4","key":"1572_CR21","doi-asserted-by":"crossref","first-page":"e10107","DOI":"10.1371\/journal.pone.0010107","volume":"5","author":"S Verma","year":"2010","unstructured":"Verma S, Kumar R, Katara GK, Singh LC, Negi NS, Ramesh V, Salotra P. Quantification of parasite load in clinical samples of leishmaniasis patients: IL-10 level correlates with parasite load in visceral leishmaniasis. PLoS One. 2010;5(4):e10107.","journal-title":"PLoS One"},{"issue":"6","key":"1572_CR22","doi-asserted-by":"crossref","first-page":"O374","DOI":"10.1111\/1469-0691.12421","volume":"20","author":"AC Vallur","year":"2014","unstructured":"Vallur AC, Duthie MS, Reinhart C, Tutterrow Y, Hamano S, Bhaskar KR, Coler RN, Mondal D, Reed SG. Biomarkers for intracellular pathogens: establishing tools as vaccine and therapeutic endpoints for visceral leishmaniasis. Clin Microbiol Infect. 2014;20(6):O374\u2013383.","journal-title":"Clin Microbiol Infect"},{"issue":"3","key":"1572_CR23","doi-asserted-by":"crossref","first-page":"163","DOI":"10.1007\/s40475-014-0027-6","volume":"1","author":"B-N Ahmed","year":"2014","unstructured":"Ahmed B-N, Nabi SG, Rahman M, Selim S, Bashar A, Rashid MM, Lira FY, Choudhury TA, Mondal D. Kala-azar (Visceral Leishmaniasis) elimination in Bangladesh: successes and challenges. Curr Trop Med Rep. 2014;1(3):163\u20139.","journal-title":"Curr Trop Med Rep"},{"key":"1572_CR24","doi-asserted-by":"crossref","unstructured":"Alonso DP, Costa DL, de Mendonca IL, Costa CH, Ribolla PE. Heterogeneity of Leishmania infantum chagasi kinetoplast DNA in Teresina (Brazil). Am J Trop Med Hyg. 2010;82(5):819\u201321.","DOI":"10.4269\/ajtmh.2010.09-0600"},{"issue":"8","key":"1572_CR25","doi-asserted-by":"crossref","first-page":"e71642","DOI":"10.1371\/journal.pone.0071642","volume":"8","author":"A Abd El Wahed","year":"2013","unstructured":"Abd El Wahed A, El-Deeb A, El-Tholoth M, Abd El Kader H, Ahmed A, Hassan S, Hoffmann B, Haas B, Shalaby MA, Hufert FT, et al. A portable reverse transcription recombinase polymerase amplification assay for rapid detection of foot-and-mouth disease virus. PLoS One. 2013;8(8):e71642.","journal-title":"PLoS One"},{"issue":"4","key":"1572_CR26","doi-asserted-by":"crossref","first-page":"1110","DOI":"10.1128\/JCM.02704-12","volume":"51","author":"M Euler","year":"2013","unstructured":"Euler M, Wang Y, Heidenreich D, Patel P, Strohmeier O, Hakenberg S, Niedrig M, Hufert FT, Weidmann M. Development of a panel of recombinase polymerase amplification assays for detection of biothreat agents. J Clin Microbiol. 2013;51(4):1110\u20137.","journal-title":"J Clin Microbiol"},{"issue":"4","key":"1572_CR27","doi-asserted-by":"crossref","first-page":"308","DOI":"10.1016\/j.jcv.2012.05.006","volume":"54","author":"M Euler","year":"2012","unstructured":"Euler M, Wang Y, Nentwich O, Piepenburg O, Hufert FT, Weidmann M. Recombinase polymerase amplification assay for rapid detection of Rift Valley fever virus. J Clin Virol. 2012;54(4):308\u201312.","journal-title":"J Clin Virol"},{"key":"1572_CR28","doi-asserted-by":"crossref","unstructured":"Euler M, Wang Y, Otto P, Tomaso H, Escudero R, Anda P, Hufert FT, Weidmann M. Recombinase polymerase amplification assay for rapid detection of Francisella tularensis. J Clin Microbiol. 2012;50(7):2234\u20138.","DOI":"10.1128\/JCM.06504-11"},{"key":"1572_CR29","doi-asserted-by":"crossref","unstructured":"Boyle DS, Lehman DA, Lillis L, Peterson D, Singhal M, Armes N, Parker M, Piepenburg O, Overbaugh J: Rapid detection of HIV-1 proviral DNA for early infant diagnosis using recombinase polymerase amplification. MBio. 2013;4(2).","DOI":"10.1128\/mBio.00135-13"},{"issue":"2","key":"1572_CR30","doi-asserted-by":"crossref","first-page":"116","DOI":"10.1016\/j.mcp.2014.11.005","volume":"29","author":"RK Daher","year":"2015","unstructured":"Daher RK, Stewart G, Boissinot M, Boudreau DK, Bergeron MG. Influence of sequence mismatches on the specificity of recombinase polymerase amplification technology. Mol Cell Probes. 2015;29(2):116\u201321.","journal-title":"Mol Cell Probes"},{"issue":"5","key":"1572_CR31","doi-asserted-by":"crossref","first-page":"970","DOI":"10.4269\/ajtmh.15-0145","volume":"93","author":"A Castellanos-Gonzalez","year":"2015","unstructured":"Castellanos-Gonzalez A, Saldarriaga OA, Tartaglino L, Gacek R, Temple E, Sparks H, Melby PC, Travi BL. A novel molecular test to diagnose canine visceral leishmaniasis at the point of care. Am J Trop Med Hyg. 2015;93(5):970\u20135.","journal-title":"Am J Trop Med Hyg"},{"issue":"8","key":"1572_CR32","doi-asserted-by":"crossref","first-page":"3841","DOI":"10.1021\/ac403971h","volume":"86","author":"TH Kim","year":"2014","unstructured":"Kim TH, Park J, Kim CJ, Cho YK. Fully integrated lab-on-a-disc for nucleic acid analysis of food-borne pathogens. Anal Chem. 2014;86(8):3841\u20138.","journal-title":"Anal Chem"},{"key":"1572_CR33","doi-asserted-by":"crossref","unstructured":"Gao CH, Ding D, Wang JY, Steverding D, Wang X, Yang YT, Shi F. Development of a LAMP assay for detection of Leishmania infantum infection in dogs using conjunctival swab samples. Parasit Vectors. 2015;8:370.","DOI":"10.1186\/s13071-015-0991-2"},{"key":"1572_CR34","doi-asserted-by":"crossref","unstructured":"Khan MG, Bhaskar KR, Salam MA, Akther T, Pluschke G, Mondal D. Diagnostic accuracy of loop-mediated isothermal amplification (LAMP) for detection of Leishmania DNA in buffy coat from visceral leishmaniasis patients. Parasit Vectors. 2012;5:280.","DOI":"10.1186\/1756-3305-5-280"},{"key":"1572_CR35","doi-asserted-by":"crossref","unstructured":"Sriworarat C, Phumee A, Mungthin M, Leelayoova S, Siriyasatien P. Development of loop-mediated isothermal amplification (LAMP) for simple detection of Leishmania infection. Parasit Vectors. 2015;8(1):591.","DOI":"10.1186\/s13071-015-1202-x"},{"key":"1572_CR36","doi-asserted-by":"crossref","unstructured":"Takagi H, Itoh M, Islam MZ, Razzaque A, Ekram AR, Hashighuchi Y, Noiri E, Kimura E. Sensitive, specific, and rapid detection of Leishmania donovani DNA by loop-mediated isothermal amplification. Am J Trop Med Hyg. 2009;81(4):578\u201382.","DOI":"10.4269\/ajtmh.2009.09-0145"},{"issue":"4","key":"1572_CR37","doi-asserted-by":"crossref","first-page":"390","DOI":"10.1016\/j.diagmicrobio.2013.01.011","volume":"75","author":"S Verma","year":"2013","unstructured":"Verma S, Avishek K, Sharma V, Negi NS, Ramesh V, Salotra P. Application of loop-mediated isothermal amplification assay for the sensitive and rapid diagnosis of visceral leishmaniasis and post-kala-azar dermal leishmaniasis. Diagn Microbiol Infect Dis. 2013;75(4):390\u20135.","journal-title":"Diagn Microbiol Infect Dis"},{"key":"1572_CR38","doi-asserted-by":"crossref","unstructured":"Adams ER, Schoone GJ, Ageed AF, Safi SE, Schallig HD. Development of a reverse transcriptase loop-mediated isothermal amplification (LAMP) assay for the sensitive detection of Leishmania parasites in clinical samples. Am J Trop Med Hyg. 2010;82(4):591\u20136.","DOI":"10.4269\/ajtmh.2010.09-0369"},{"issue":"12","key":"1572_CR39","doi-asserted-by":"crossref","first-page":"E63","DOI":"10.1093\/nar\/28.12.e63","volume":"28","author":"T Notomi","year":"2000","unstructured":"Notomi T, Okayama H, Masubuchi H, Yonekawa T, Watanabe K, Amino N, Hase T. Loop-mediated isothermal amplification of DNA. Nucleic Acids Res. 2000;28(12):E63.","journal-title":"Nucleic Acids Res"},{"key":"1572_CR40","doi-asserted-by":"crossref","unstructured":"Snounou G, Viriyakosol S, Zhu XP, Jarra W, Pinheiro L, do Rosario VE, et al. High sensitivity of detection of human malaria parasites by the use of nested polymerase chain reaction. Mol Biochem Parasitol. 1993;61(2):315\u201320.","DOI":"10.1016\/0166-6851(93)90077-B"},{"issue":"Pt 10","key":"1572_CR41","doi-asserted-by":"crossref","first-page":"1356","DOI":"10.1099\/jmm.0.47265-0","volume":"56","author":"S Haldar","year":"2007","unstructured":"Haldar S, Chakravorty S, Bhalla M, De Majumdar S, Tyagi JS. Simplified detection of Mycobacterium tuberculosis in sputum using smear microscopy and PCR with molecular beacons. J Med Microbiol. 2007;56(Pt 10):1356\u201362.","journal-title":"J Med Microbiol"}],"container-title":["Parasites &amp; Vectors"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/link.springer.com\/content\/pdf\/10.1186\/s13071-016-1572-8","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2019,9,7]],"date-time":"2019-09-07T12:49:32Z","timestamp":1567860572000},"score":1,"resource":{"primary":{"URL":"http:\/\/parasitesandvectors.biomedcentral.com\/articles\/10.1186\/s13071-016-1572-8"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2016,5,13]]},"references-count":41,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2016,12]]}},"alternative-id":["1572"],"URL":"https:\/\/doi.org\/10.1186\/s13071-016-1572-8","relation":{},"ISSN":["1756-3305"],"issn-type":[{"value":"1756-3305","type":"electronic"}],"subject":[],"published":{"date-parts":[[2016,5,13]]},"article-number":"281"}}