{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,23]],"date-time":"2026-10-23T20:45:23Z","timestamp":1792788323086,"version":"4.5.0"},"reference-count":29,"publisher":"Springer Science and Business Media LLC","issue":"1","funder":[{"name":"Minas Gerais State Research Support Foundation- FAPEMIG","award":["00240-13"],"award-info":[{"award-number":["00240-13"]}]},{"name":"Minas Gerais State Research Support Foundation- FAPEMIG","award":["00242-12"],"award-info":[{"award-number":["00242-12"]}]},{"name":"Brazilian Council for Scientific and Technological Development CNPq\/ DECIT","award":["440615\/2016"],"award-info":[{"award-number":["440615\/2016"]}]},{"name":"Brazilian Council for Scientific and Technological Development- CNPq","award":["308849\/2015-9"],"award-info":[{"award-number":["308849\/2015-9"]}]},{"name":"Brazilian Council for Scientific and Technological Development- CNPq","award":["305512\/2014-5"],"award-info":[{"award-number":["305512\/2014-5"]}]},{"name":"Brazilian Council for Scientific and Technological Development- CNPq (Science without Borders)","award":["400704\/2014-5"],"award-info":[{"award-number":["400704\/2014-5"]}]},{"name":"Brazilian Council for Scientific and Technological Development (CNPq) INCT INCT de Entomologia Molecular","award":["465678\/2014-9."],"award-info":[{"award-number":["465678\/2014-9."]}]}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Parasites Vectors"],"published-print":{"date-parts":[[2017,12]]},"DOI":"10.1186\/s13071-017-2286-2","type":"journal-article","created":{"date-parts":[[2017,7,20]],"date-time":"2017-07-20T08:04:07Z","timestamp":1500537847000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":23,"title":["Zika virus transmission to mouse ear by mosquito bite: a laboratory model that replicates the natural transmission process"],"prefix":"10.1186","volume":"10","author":[{"given":"Nagila Francinete Costa","family":"Secundino","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Barbara Aparecida","family":"Chaves","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alessandra Silva","family":"Orfano","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Karine Renata Dias","family":"Silveira","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nilton Barnabe","family":"Rodrigues","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thais Bonif\u00e1cio","family":"Campolina","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rafael","family":"Nacif-Pimenta","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Luiz Eduardo Martinez","family":"Villegas","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Breno Melo","family":"Silva","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marcus Vin\u00edcius Guimar\u00e3es","family":"Lacerda","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Douglas Eric","family":"Norris","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paulo Filemon Paolucci","family":"Pimenta","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2017,7,20]]},"reference":[{"key":"2286_CR1","doi-asserted-by":"crossref","unstructured":"Lessler J, Chaisson LH, Kucirka LM, Bi Q, Grantz K, Salje H, et al. Assessing the global threat from Zika virus. Science. 2016;353:aaf8160.","DOI":"10.1126\/science.aaf8160"},{"key":"2286_CR2","volume-title":"Monitoramento dos casos de dengue, febre de chikungunya e febre pelo v\u00edrus Zika at\u00e9 a Semana Epidemiol\u00f3gica 21","author":"M Sa\u00fade da","year":"2016","unstructured":"Ministerio da Sa\u00fade. Monitoramento dos casos de dengue, febre de chikungunya e febre pelo v\u00edrus Zika at\u00e9 a Semana Epidemiol\u00f3gica 21; 2016."},{"key":"2286_CR3","doi-asserted-by":"crossref","first-page":"43","DOI":"10.1016\/S0035-9203(03)00006-3","volume":"98","author":"R Louren\u00e7o-de-Oliveira","year":"2004","unstructured":"Louren\u00e7o-de-Oliveira R, Vazeille M, Filippis AMB, Failloux AB. Aedes aegypti in Brazil: genetically differentiated populations with high susceptibility to dengue and yellow fever viruses. Trans R Soc Trop Med Hyg. 2004;98:43\u201354.","journal-title":"Trans R Soc Trop Med Hyg"},{"key":"2286_CR4","doi-asserted-by":"crossref","first-page":"1362","DOI":"10.4269\/ajtmh.16-0111","volume":"94","author":"SL Rossi","year":"2016","unstructured":"Rossi SL, Tesh RB, Azar SR, Muruato AE, Hanley KA, Auguste AJ, et al. Characterization of a novel murine model to study Zika virus. Am J Trop Med Hyg. 2016;94:1362\u20139.","journal-title":"Am J Trop Med Hyg"},{"key":"2286_CR5","doi-asserted-by":"crossref","first-page":"267","DOI":"10.1038\/nature18296","volume":"534","author":"FR Cugola","year":"2016","unstructured":"Cugola FR, Fernandes IR, Russo FB, Freitas BC, Dias JL, Guimaraes KP, et al. The Brazilian Zika virus strain causes birth defects in experimental models. Nature. 2016;534:267\u201371.","journal-title":"Nature"},{"key":"2286_CR6","doi-asserted-by":"crossref","first-page":"12204","DOI":"10.1038\/ncomms12204","volume":"7","author":"DM Dudley","year":"2016","unstructured":"Dudley DM, Aliota MT, Mohr EL, Weiler AM, Lehrer-Brey G, Weisgrau KL, et al. A rhesus macaque model of Asian-lineage Zika virus infection. Nat Commun. 2016;7:12204.","journal-title":"Nat Commun"},{"issue":"Suppl. 5","key":"2286_CR7","volume":"10","author":"MJ Aman","year":"2016","unstructured":"Aman MJ, Kashanchi F. Zika virus: a new animal model for an arbovirus. PLoS Negl Trop Dis. 2016;10(Suppl. 5):e0004702.","journal-title":"PLoS Negl Trop Dis"},{"issue":"Suppl. 5","key":"2286_CR8","volume":"10","author":"SD Dowall","year":"2016","unstructured":"Dowall SD, Graham VA, Rayner E, Atkinson B, Hall G, Watson RJ, et al. A susceptible mouse model for Zika virus infection. PLoS Negl Trop Dis. 2016;10(Suppl. 5):e0004658.","journal-title":"PLoS Negl Trop Dis"},{"key":"2286_CR9","doi-asserted-by":"publisher","unstructured":"Pawitwar SS, Dhar S, Tiwari S, Ojha CR, Lapierre J, Martins K, et al. Overview on the current status of Zika virus pathogenesis and animal related research. J NeuroImmune Pharmacol. 2017; doi: 10.1007\/s11481-017-9743-8 .","DOI":"10.1007\/s11481-017-9743-8"},{"issue":"6","key":"2286_CR10","doi-asserted-by":"crossref","first-page":"1455","DOI":"10.1016\/j.immuni.2016.06.002","volume":"44","author":"M Pingen","year":"2016","unstructured":"Pingen M, Bryden SR, Pondeville E, Schnettler E, Kohl A, Merits A, et al. Host inflammatory response to mosquito bites enhances the severity of arbovirus infection. Immunity. 2016;44(6):1455\u201369.","journal-title":"Immunity"},{"issue":"5495","key":"2286_CR11","doi-asserted-by":"crossref","first-page":"1351","DOI":"10.1126\/science.290.5495.1351","volume":"290","author":"S Kamhawi","year":"2000","unstructured":"Kamhawi S, Belkaid Y, Modi G, Rowton E, Sacks D. Protection against cutaneous leishmaniasis resulting from bites of uninfected sand flies. Science. 2000;290(5495):1351\u20134.","journal-title":"Science"},{"issue":"5891","key":"2286_CR12","doi-asserted-by":"crossref","first-page":"970","DOI":"10.1126\/science.1159194","volume":"321","author":"NC Peters","year":"2008","unstructured":"Peters NC, Egen JG, Secundino N, Debrabant A, Kimblin N, Kamhawi S, et al. In vivo imaging reveals an essential role for neutrophils in leishmaniasis transmitted by sand flies. Science. 2008;321(5891):970\u20134.","journal-title":"Science"},{"key":"2286_CR13","doi-asserted-by":"crossref","first-page":"691","DOI":"10.1016\/j.meegid.2014.07.028","volume":"28","author":"M Abdeladhim","year":"2014","unstructured":"Abdeladhim M, Kamhawi S, Valenzuela JG. What's behind a sand fly bite? The profound effect of sand fly saliva on host hemostasis, inflammation and immunity. Infect Genet Evol. 2014;28:691\u2013703.","journal-title":"Infect Genet Evol"},{"key":"2286_CR14","doi-asserted-by":"crossref","first-page":"8880","DOI":"10.1128\/JVI.00354-15","volume":"89","author":"R Hamel","year":"2015","unstructured":"Hamel R, Dejarnac O, Wichit S, Ekchariyawat P, Neyret A, Luplertlop N, et al. Biology of Zika virus infection in human skin cells. J Virol. 2015;89:8880\u201396.","journal-title":"J Virol"},{"key":"2286_CR15","doi-asserted-by":"crossref","first-page":"238","DOI":"10.1016\/0035-9203(56)90029-3","volume":"50","author":"JP Boorman","year":"1956","unstructured":"Boorman JP, Porterfield JS. A simple technique for infection of mosquitoes with viruses; transmission of Zika virus. Trans R Soc Trop Med Hyg. 1956;50:238\u201342.","journal-title":"Trans R Soc Trop Med Hyg"},{"key":"2286_CR16","doi-asserted-by":"crossref","unstructured":"Ross RW. A laboratory technique for studying the insect transmission of animal viruses, employing a bat-wing membrane, demonstrated with two African viruses. J Hyg (Lond). 1956;54:192\u2013200.","DOI":"10.1017\/S0022172400044454"},{"key":"2286_CR17","doi-asserted-by":"crossref","unstructured":"Styer LM, Kent KA, Albright RG, Bennett CJ, Kramer LD, Bernard KA. Mosquitoes inoculate high doses of West Nile virus as they probe and feed on live hosts. PLoS Pathog. 2007:31262\u201370.","DOI":"10.1371\/journal.ppat.0030132"},{"key":"2286_CR18","doi-asserted-by":"crossref","first-page":"e00032","DOI":"10.1128\/genomeA.00032-16","volume":"4","author":"MS Cunha","year":"2016","unstructured":"Cunha MS, Esposito DL, Rocco IM, Maeda AY, Vasami FG, Nogueira JS, et al. First complete genome sequence of Zika virus (Flaviviridae, Flavivirus) from an autochthonous transmission in Brazil. Genome Announc. 2016;4:e00032\u201316.","journal-title":"Genome Announc"},{"key":"2286_CR19","doi-asserted-by":"crossref","first-page":"320","DOI":"10.1186\/1756-3305-7-320","volume":"7","author":"CM Goncalves","year":"2014","unstructured":"Goncalves CM, Melo FF, Bezerra JM, Chaves BA, Silva BM, Silva LD, et al. Distinct variation in vector competence among nine field populations of Aedes aegypti from a Brazilian dengue-endemic risk city. Parasit Vectors. 2014;7:320.","journal-title":"Parasit Vectors"},{"key":"2286_CR20","unstructured":"Booth C, Bergan T, Bennett PM, Brown AJP, Colwell RR, Craig AG, et al. Methods in microbiology. Immunology of infection Vol. 32;2002."},{"key":"2286_CR21","unstructured":"O'Reilly DR, Miller LK, Luckow VA. Baculovirus expression vectors: a laboratory manual. In: Virus Methods. Oxford University Press. 1994. P. 124\u2013138."},{"key":"2286_CR22","doi-asserted-by":"publisher","DOI":"10.3791\/54767","volume":"114","author":"D Contreras","year":"2016","unstructured":"Contreras D, Arumugaswami V. Zika virus infectious cell culture system and the in vitro prophylactic effect of interferons. J Vis Exp. 2016;114:e54767. doi: 10.3791\/54767 .","journal-title":"J Vis Exp"},{"key":"2286_CR23","doi-asserted-by":"crossref","unstructured":"Dutra HL, Rocha MN, Dias FB, Mansur SB, Caragata EP, Moreira LA. Wolbachia blocks currently circulating Zika virus isolates in Brazilian Aedes aegypti mosquitoes. Cell Host Microbe. 2016;19:771\u20134.","DOI":"10.1016\/j.chom.2016.04.021"},{"key":"2286_CR24","doi-asserted-by":"crossref","unstructured":"Lanciotti RS, Kosoy OL, Laven JJ, Velez JO, Lambert AJ, Johnson AJ, et al. Genetic and serologic properties of Zika virus associated with an epidemic, Yap state, Micronesia, 2007. Emerg Infect Dis. 2008;14:1232\u20139.","DOI":"10.3201\/eid1408.080287"},{"key":"2286_CR25","doi-asserted-by":"crossref","first-page":"655","DOI":"10.1590\/0074-02760160332","volume":"111","author":"A Ferreira-de-Brito","year":"2016","unstructured":"Ferreira-de-Brito A, Ribeiro IP, Miranda RM, Fernandes RS, Campos SS, Silva KA, et al. First detection of natural infection of Aedes aegypti with Zika virus in Brazil and throughout South America. Mem Inst Oswaldo Cruz. 2016;111:655\u20138.","journal-title":"Mem Inst Oswaldo Cruz"},{"key":"2286_CR26","doi-asserted-by":"crossref","unstructured":"Secundino NF, de Freitas VC, Monteiro CC, Pires A-CA, David BA, Pimenta PF. The transmission of Leishmania infantum chagasi by the bite of the Lutzomyia longipalpis to two different vertebrates. Parasit Vectors. 2012;5:20.","DOI":"10.1186\/1756-3305-5-20"},{"key":"2286_CR27","doi-asserted-by":"crossref","unstructured":"Kimblin N, Peters N, Debrabant A, Secundino N, Egen J, Lawyer P, et al. Quantification of the infectious dose of Leishmania major transmitted to the skin by single sand flies. Proc Natl Acad Sci USA. 2008;105(29):10125\u201330.","DOI":"10.1073\/pnas.0802331105"},{"key":"2286_CR28","doi-asserted-by":"crossref","first-page":"75","DOI":"10.1016\/j.virol.2017.04.009","volume":"507","author":"V Raquin","year":"2017","unstructured":"Raquin V, Lambrechts L. Dengue virus replicates and accumulates in Aedes aegypti salivary glands. Virology. 2017;507:75\u201381.","journal-title":"Virology"},{"key":"2286_CR29","doi-asserted-by":"crossref","DOI":"10.1371\/journal.ppat.1000484","volume":"5","author":"NC Peters","year":"2009","unstructured":"Peters NC, Kimblin N, Secundino N, Kamhawi S, Lawyer P, Sacks DL. Vector transmission of Leishmania abrogates vaccine-induced protective immunity. PLoS Pathog. 2009;5:e1000484.","journal-title":"PLoS Pathog"}],"container-title":["Parasites & Vectors"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/link.springer.com\/content\/pdf\/10.1186\/s13071-017-2286-2.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2019,9,30]],"date-time":"2019-09-30T23:48:07Z","timestamp":1569887287000},"score":1,"resource":{"primary":{"URL":"http:\/\/parasitesandvectors.biomedcentral.com\/articles\/10.1186\/s13071-017-2286-2"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2017,7,20]]},"references-count":29,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2017,12]]}},"alternative-id":["2286"],"URL":"https:\/\/doi.org\/10.1186\/s13071-017-2286-2","relation":{},"ISSN":["1756-3305"],"issn-type":[{"value":"1756-3305","type":"electronic"}],"subject":[],"published":{"date-parts":[[2017,7,20]]},"article-number":"346"}}