{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,10]],"date-time":"2026-03-10T04:54:12Z","timestamp":1773118452208,"version":"3.50.1"},"reference-count":36,"publisher":"Springer Science and Business Media LLC","issue":"1","funder":[{"name":"National Natural Science Foundation of China (CN)","award":["81572022"],"award-info":[{"award-number":["81572022"]}]},{"name":"National Key R&D Program of China","award":["2017YFD0500400"],"award-info":[{"award-number":["2017YFD0500400"]}]}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Parasites Vectors"],"published-print":{"date-parts":[[2017,12]]},"DOI":"10.1186\/s13071-017-2529-2","type":"journal-article","created":{"date-parts":[[2017,11,23]],"date-time":"2017-11-23T12:06:51Z","timestamp":1511438811000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":13,"title":["A mechanistic study of Toxoplasma gondii ROP18 inhibiting differentiation of C17.2 neural stem cells"],"prefix":"10.1186","volume":"10","author":[{"given":"Xian","family":"Zhang","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rui","family":"Su","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zhengyang","family":"Cheng","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wanbo","family":"Zhu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yelin","family":"Li","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yongzhong","family":"Wang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jian","family":"Du","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yihong","family":"Cai","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Qingli","family":"Luo","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jilong","family":"Shen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Li","family":"Yu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2017,11,23]]},"reference":[{"issue":"12\u201313","key":"2529_CR1","doi-asserted-by":"crossref","first-page":"1217","DOI":"10.1016\/S0020-7519(00)00124-7","volume":"30","author":"AM Tenter","year":"2000","unstructured":"Tenter AM, Heckeroth AR, Weiss LM. Toxoplasma gondii: from animals to humans. Int J Parasitol. 2000;30(12\u201313):1217\u201358.","journal-title":"Int J Parasitol"},{"key":"2529_CR2","volume-title":"Toxoplasmosis of animals and humans","author":"JP Dubey","year":"2010","unstructured":"Dubey JP. Toxoplasmosis of animals and humans. Second ed. Florida: CRC Press; 2010.","edition":"Second"},{"issue":"10","key":"2529_CR3","doi-asserted-by":"crossref","first-page":"634","DOI":"10.1046\/j.1469-0691.2002.00485.x","volume":"8","author":"D Hill","year":"2002","unstructured":"Hill D, Dubey JP. Toxoplasma gondii: transmission, diagnosis and prevention. Clin Microbiol Infect. 2002;8(10):634\u201340.","journal-title":"Clin Microbiol Infect"},{"key":"2529_CR4","first-page":"1","volume":"583","author":"O Andreoletti","year":"2007","unstructured":"Andreoletti O, Budka H, Buncic S, Colin P, Collins DJ, De Koeijer A, et al. Scientific opinion of the panel on biological hazards on a request from EFSA on surveillance and monitoring of Toxoplasma in humans, foods and animals. EFSA J. 2007;583:1\u201364.","journal-title":"EFSA J"},{"issue":"3","key":"2529_CR5","doi-asserted-by":"crossref","first-page":"214","DOI":"10.1016\/j.siny.2007.01.011","volume":"12","author":"E Petersen","year":"2007","unstructured":"Petersen E. Toxoplasmosis. Semin Fetal Neonatal Med. 2007;12(3):214\u201323.","journal-title":"Semin Fetal Neonatal Med"},{"issue":"6","key":"2529_CR6","doi-asserted-by":"crossref","first-page":"582","DOI":"10.1016\/j.mib.2007.09.013","volume":"10","author":"PJ Bradley","year":"2007","unstructured":"Bradley PJ, Sibley LD. Rhoptries: an arsenal of secreted virulence factors. Curr Opin Microbiol. 2007;10(6):582\u20137.","journal-title":"Curr Opin Microbiol"},{"issue":"1","key":"2529_CR7","doi-asserted-by":"crossref","first-page":"79","DOI":"10.1038\/nrmicro1800","volume":"6","author":"JC Boothroyd","year":"2008","unstructured":"Boothroyd JC, Dubremetz JF. Kiss and spit: the dual roles of Toxoplasma rhoptries. Nat Rev Microbiol. 2008;6(1):79\u201388.","journal-title":"Nat Rev Microbiol"},{"issue":"5806","key":"2529_CR8","doi-asserted-by":"crossref","first-page":"1780","DOI":"10.1126\/science.1133690","volume":"314","author":"JP Saeij","year":"2006","unstructured":"Saeij JP, Boyle JP, Coller S, Taylor S, Sibley LD, Brooke-Powell ET, et al. Polymorphic secreted kinases are key virulence factors in toxoplasmosis. Science. 2006;314(5806):1780\u20133.","journal-title":"Science"},{"key":"2529_CR9","doi-asserted-by":"crossref","unstructured":"Taylor S, Barragan A, Su C, Fux B, Fentress SJ, Tang K, et al. A secreted serine-threonine kinase determines virulence in the eukaryotic pathogen Toxoplasma gondii. Science. 2006;314(5806):1776\u201380.","DOI":"10.1126\/science.1133643"},{"issue":"7","key":"2529_CR10","doi-asserted-by":"crossref","first-page":"1533","DOI":"10.1084\/jem.20101660","volume":"208","author":"M Yamamoto","year":"2011","unstructured":"Yamamoto M, Ma JS, Mueller C, Kamiyama N, Saiga H, Kubo E, et al. ATF6beta is a host cellular target of the Toxoplasma gondii virulence factor ROP18. J Exp Med. 2011;208(7):1533\u201346.","journal-title":"J Exp Med"},{"key":"2529_CR11","doi-asserted-by":"crossref","unstructured":"Yamamoto M, Takeda K. Inhibition of ATF6beta-dependent host adaptive immune response by a Toxoplasma virulence factor ROP18. Virulence. 2012;3(1):77\u201380.","DOI":"10.4161\/viru.3.1.18340"},{"issue":"18","key":"2529_CR12","doi-asserted-by":"crossref","first-page":"12578","DOI":"10.1074\/jbc.M113.544718","volume":"289","author":"J Du","year":"2014","unstructured":"Du J, An R, Chen L, Shen Y, Chen Y, Cheng L, et al. Toxoplasma gondii virulence factor ROP18 inhibits the host NF-kappaB pathway by promoting p65 degradation. J Biol Chem. 2014;289(18):12578\u201392.","journal-title":"J Biol Chem"},{"issue":"6","key":"2529_CR13","doi-asserted-by":"crossref","first-page":"484","DOI":"10.1016\/j.chom.2010.11.005","volume":"8","author":"SJ Fentress","year":"2010","unstructured":"Fentress SJ, Behnke MS, Dunay IR, Mashayekhi M, Rommereim LM, Fox BA, et al. Phosphorylation of immunity-related GTPases by a Toxoplasma gondii-secreted kinase promotes macrophage survival and virulence. Cell Host Microbe. 2010;8(6):484\u201395.","journal-title":"Cell Host Microbe"},{"issue":"12","key":"2529_CR14","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pbio.1000576","volume":"8","author":"T Steinfeldt","year":"2010","unstructured":"Steinfeldt T, Konen-Waisman S, Tong L, Pawlowski N, Lamkemeyer T, Sibley LD, et al. Phosphorylation of mouse immunity-related GTPase (IRG) resistance proteins is an evasion strategy for virulent Toxoplasma gondii. PLoS Biol. 2010;8(12):e1000576.","journal-title":"PLoS Biol"},{"issue":"4","key":"2529_CR15","doi-asserted-by":"crossref","first-page":"234","DOI":"10.1007\/s12250-012-3250-0","volume":"27","author":"X Sun","year":"2012","unstructured":"Sun X, Guan Y, Li F, Li X, Wang X, Guan Z, et al. Effects of rat cytomegalovirus on the nervous system of the early rat embryo. Virol Sin. 2012;27(4):234\u201340.","journal-title":"Virol Sin"},{"issue":"11","key":"2529_CR16","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0049700","volume":"7","author":"L D'Aiuto","year":"2012","unstructured":"D'Aiuto L, Di Maio R, Heath B, Raimondi G, Milosevic J, Watson AM, et al. Human induced pluripotent stem cell-derived models to investigate human cytomegalovirus infection in neural cells. PLoS One. 2012;7(11):e49700.","journal-title":"PLoS One"},{"issue":"1","key":"2529_CR17","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0016211","volume":"6","author":"MB Mutnal","year":"2011","unstructured":"Mutnal MB, Cheeran MC, Hu S, Lokensgard JR. Murine cytomegalovirus infection of neural stem cells alters neurogenesis in the developing brain. PLoS One. 2011;6(1):e16211.","journal-title":"PLoS One"},{"key":"2529_CR18","doi-asserted-by":"crossref","unstructured":"Zhou J, Gan X, Wang Y, Zhang X, Ding X, Chen L, et al. Toxoplasma gondii prevalent in China induce weaker apoptosis of neural stem cells C17.2 via endoplasmic reticulum stress (ERS) signaling pathways. Parasit Vectors. 2015;8:73.","DOI":"10.1186\/s13071-015-0670-3"},{"key":"2529_CR19","doi-asserted-by":"crossref","unstructured":"Wang T, Zhou J, Gan X, Wang H, Ding X, Chen L, et al. Toxoplasma gondii induces apoptosis of neural stem cells via endoplasmic reticulum stress pathway. Parasitology. 2014;141(7):988\u201395.","DOI":"10.1017\/S0031182014000183"},{"issue":"1","key":"2529_CR20","doi-asserted-by":"crossref","first-page":"187","DOI":"10.1016\/j.bbrc.2016.03.076","volume":"473","author":"X Gan","year":"2016","unstructured":"Gan X, Zhang X, Cheng Z, Chen L, Ding X, Du J, et al. Toxoplasma gondii inhibits differentiation of C17.2 neural stem cells through Wnt\/beta-catenin signaling pathway. Biochem Biophys Res Commun. 2016;473(1):187\u201393.","journal-title":"Biochem Biophys Res Commun"},{"issue":"1","key":"2529_CR21","doi-asserted-by":"crossref","first-page":"193","DOI":"10.1007\/s00441-007-0476-5","volume":"331","author":"TM Michaelidis","year":"2008","unstructured":"Michaelidis TM, Lie DC. Wnt signaling and neural stem cells: caught in the Wnt web. Cell Tissue Res. 2008;331(1):193\u2013210.","journal-title":"Cell Tissue Res"},{"issue":"3","key":"2529_CR22","doi-asserted-by":"crossref","first-page":"237","DOI":"10.1016\/j.toxlet.2011.03.023","volume":"203","author":"RA Rocha","year":"2011","unstructured":"Rocha RA, Gimeno-Alcaniz JV, Martin-Ibanez R, Canals JM, Velez D, Devesa V. Arsenic and fluoride induce neural progenitor cell apoptosis. Toxicol Lett. 2011;203(3):237\u201344.","journal-title":"Toxicol Lett"},{"issue":"6","key":"2529_CR23","doi-asserted-by":"crossref","first-page":"597","DOI":"10.1002\/1097-4547(20000915)61:6<597::AID-JNR3>3.0.CO;2-L","volume":"61","author":"LC Doering","year":"2000","unstructured":"Doering LC, Snyder EY. Cholinergic expression by a neural stem cell line grafted to the adult medial septum\/diagonal band complex. J Neurosci Res. 2000;61(6):597\u2013604.","journal-title":"J Neurosci Res"},{"key":"2529_CR24","doi-asserted-by":"crossref","first-page":"75","DOI":"10.1385\/1-59259-409-3:75","volume-title":"E coli plasmid vectors: methods and applications","author":"S Ehrt","year":"2003","unstructured":"Ehrt S, Schnappinger D. Isolation of plasmids from E. coli by alkaline lysis. In: Casali N, Preston A, Totowa NJ, editors. E coli plasmid vectors: methods and applications. New York: Humana Press; 2003. p. 75\u20138."},{"issue":"2","key":"2529_CR25","doi-asserted-by":"crossref","first-page":"107","DOI":"10.1016\/S0166-0934(01)00316-0","volume":"96","author":"DD LaBarre","year":"2001","unstructured":"LaBarre DD, Lowy RJ. Improvements in methods for calculating virus titer estimates from TCID50 and plaque assays. J Virol Methods. 2001;96(2):107\u201326.","journal-title":"J Virol Methods"},{"issue":"7","key":"2529_CR26","doi-asserted-by":"crossref","first-page":"939","DOI":"10.1111\/j.1462-5822.2010.01443.x","volume":"12","author":"A Khaminets","year":"2010","unstructured":"Khaminets A, Hunn JP, Konen-Waisman S, Zhao YO, Preukschat D, Coers J, et al. Coordinated loading of IRG resistance GTPases on to the Toxoplasma gondii parasitophorous vacuole. Cell Microbiol. 2010;12(7):939\u201361.","journal-title":"Cell Microbiol"},{"key":"2529_CR27","doi-asserted-by":"crossref","unstructured":"Howard JC, Hunn JP, Steinfeldt T. The IRG protein-based resistance mechanism in mice and its relation to virulence in Toxoplasma gondii. Curr Opin Microbiol. 2011;14(4):414\u201321.","DOI":"10.1016\/j.mib.2011.07.002"},{"issue":"3","key":"2529_CR28","first-page":"234","volume":"32","author":"X Zhang","year":"2016","unstructured":"Zhang X, Gan X, Cheng Z, Yu L. Construction of the Toxoplasma ROP18 recombinant adenovirus vector and of its effect on the apoptosis of C17.2 stem cells. Chin J Zoonoses. 2016;32(3):234\u20139.","journal-title":"Chin J Zoonoses"},{"key":"2529_CR29","doi-asserted-by":"crossref","unstructured":"Wan L, Gong L, Wang W, An R, Zheng M, Jiang Z, et al. T. gondii rhoptry protein ROP18 induces apoptosis of neural cells via endoplasmic reticulum stress pathway. Parasit Vectors. 2015;8:554.","DOI":"10.1186\/s13071-015-1103-z"},{"issue":"2","key":"2529_CR30","doi-asserted-by":"crossref","first-page":"207","DOI":"10.1007\/s12015-013-9486-8","volume":"10","author":"JK Van Camp","year":"2014","unstructured":"Van Camp JK, Beckers S, Zegers D, Van Hul W. Wnt signaling and the control of human stem cell fate. Stem Cell Rev. 2014;10(2):207\u201329.","journal-title":"Stem Cell Rev"},{"issue":"5","key":"2529_CR31","doi-asserted-by":"crossref","first-page":"867","DOI":"10.1083\/jcb.200209039","volume":"159","author":"L Hari","year":"2002","unstructured":"Hari L, Brault V, Kleber M, Lee HY, Ille F, Leimeroth R, et al. Lineage-specific requirements of beta-catenin in neural crest development. J Cell Biol. 2002;159(5):867\u201380.","journal-title":"J Cell Biol"},{"issue":"8","key":"2529_CR32","doi-asserted-by":"crossref","first-page":"1253","DOI":"10.1242\/dev.128.8.1253","volume":"128","author":"V Brault","year":"2001","unstructured":"Brault V, Moore R, Kutsch S, Ishibashi M, Rowitch DH, McMahon AP, et al. Inactivation of the beta-catenin gene by Wnt1-Cre-mediated deletion results in dramatic brain malformation and failure of craniofacial development. Development. 2001;128(8):1253\u201364.","journal-title":"Development"},{"key":"2529_CR33","doi-asserted-by":"crossref","unstructured":"Zechner D, Fujita Y, Hulsken J, Muller T, Walther I, Taketo MM, et al. Beta-catenin signals regulate cell growth and the balance between progenitor cell expansion and differentiation in the nervous system. Dev Biol. 2003;258(2):406\u201318.","DOI":"10.1016\/S0012-1606(03)00123-4"},{"issue":"12","key":"2529_CR34","doi-asserted-by":"crossref","first-page":"2791","DOI":"10.1242\/dev.01165","volume":"131","author":"Y Hirabayashi","year":"2004","unstructured":"Hirabayashi Y, Itoh Y, Tabata H, Nakajima K, Akiyama T, Masuyama N, Gotoh Y. The Wnt\/beta-catenin pathway directs neuronal differentiation of cortical neural precursor cells. Development. 2004;131(12):2791\u2013801.","journal-title":"Development"},{"issue":"2","key":"2529_CR35","doi-asserted-by":"crossref","first-page":"429","DOI":"10.1128\/MCB.20.2.429-440.2000","volume":"20","author":"ME Massari","year":"2000","unstructured":"Massari ME, Murre C. Helix-Loop-Helix proteins: regulators of transcription in eucaryotic organisms. Mol Cell Biol. 2000;20(2):429\u201340.","journal-title":"Mol Cell Biol"},{"issue":"1","key":"2529_CR36","doi-asserted-by":"crossref","first-page":"26","DOI":"10.1016\/S0959-4388(02)00286-6","volume":"12","author":"C Schuurmans","year":"2002","unstructured":"Schuurmans C, Guillemot F. Molecular mechanisms underlying cell fate specification in the developing telencephalon. Curr Opin Neurobiol. 2002;12(1):26\u201334.","journal-title":"Curr Opin Neurobiol"}],"container-title":["Parasites &amp; Vectors"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/link.springer.com\/content\/pdf\/10.1186\/s13071-017-2529-2.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,8,8]],"date-time":"2022-08-08T04:12:56Z","timestamp":1659931976000},"score":1,"resource":{"primary":{"URL":"https:\/\/parasitesandvectors.biomedcentral.com\/articles\/10.1186\/s13071-017-2529-2"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2017,11,23]]},"references-count":36,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2017,12]]}},"alternative-id":["2529"],"URL":"https:\/\/doi.org\/10.1186\/s13071-017-2529-2","relation":{},"ISSN":["1756-3305"],"issn-type":[{"value":"1756-3305","type":"electronic"}],"subject":[],"published":{"date-parts":[[2017,11,23]]},"article-number":"585"}}