{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,9]],"date-time":"2026-06-09T07:45:20Z","timestamp":1780991120482,"version":"3.54.1"},"reference-count":33,"publisher":"Springer Science and Business Media LLC","issue":"1","license":[{"start":{"date-parts":[[2019,1,8]],"date-time":"2019-01-08T00:00:00Z","timestamp":1546905600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"BMGF through DNDi, Geneva","award":["ID OPP1119042"],"award-info":[{"award-number":["ID OPP1119042"]}]}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Parasites Vectors"],"published-print":{"date-parts":[[2019,12]]},"DOI":"10.1186\/s13071-018-3282-x","type":"journal-article","created":{"date-parts":[[2019,1,8]],"date-time":"2019-01-08T10:24:18Z","timestamp":1546943058000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":16,"title":["Effect of flubendazole on developing stages of Loa loa in vitro and in vivo: a new approach for screening filaricidal agents"],"prefix":"10.1186","volume":"12","author":[{"given":"Fanny Fri","family":"Fombad","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Abdel Jelil","family":"Njouendou","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Patrick Chounna","family":"Ndongmo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Manuel","family":"Ritter","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Valerine C.","family":"Chunda","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Haelly M.","family":"Metuge","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Narcisse Victor T.","family":"Gandjui","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peter","family":"Enyong","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Flobert","family":"Njiokou","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Achim","family":"Hoerauf","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Charles D.","family":"Mackenzie","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0022-8366","authenticated-orcid":false,"given":"Samuel","family":"Wanji","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2019,1,8]]},"reference":[{"key":"3282_CR1","doi-asserted-by":"publisher","first-page":"983","DOI":"10.1016\/j.trstmh.2008.03.022","volume":"102","author":"JJ Padgett","year":"2008","unstructured":"Padgett JJ, Jacobsen KH. Loiasis: African eye worm. Trans R Soc Trop Med Hyg. 2008;102:983\u20139.","journal-title":"Trans R Soc Trop Med Hyg"},{"key":"3282_CR2","doi-asserted-by":"publisher","first-page":"279","DOI":"10.1016\/0169-4758(88)90019-1","volume":"4","author":"M Pinder","year":"1988","unstructured":"Pinder M. Loa loa - a neglected filaria. Parasitol Today. 1988;4:279\u201384.","journal-title":"Parasitol Today"},{"key":"3282_CR3","first-page":"338","volume":"32","author":"A Mongin","year":"1770","unstructured":"Mongin A. Observations sur un ver trouv\u00e9 sous la conjonctive \u00e0 Maribarou, \u00eele Saint-Dominique. J Med Chir Pharm Paris. 1770;32:338\u20139.","journal-title":"J Med Chir Pharm Paris"},{"key":"3282_CR4","doi-asserted-by":"publisher","first-page":"18","DOI":"10.1016\/S0140-6736(96)11094-1","volume":"350","author":"J Gardon","year":"1997","unstructured":"Gardon J, Gardon-Wendel N, Demanga N, Kamgno J, Chippaux J-P, Boussinesq M. Serious reactions after mass treatment of onchocerciasis with ivermectin in an area endemic for Loa loa infection. Lancet. 1997;350:18\u201322.","journal-title":"Lancet"},{"key":"3282_CR5","doi-asserted-by":"publisher","first-page":"448","DOI":"10.1016\/0169-4758(96)40006-0","volume":"12","author":"J-P Chippaux","year":"1996","unstructured":"Chippaux J-P, Boussinesq M, Gardon J, Gardon-Wendel N, Ernould J-C. Severe adverse reaction risks during mass treatment with ivermectin in loiasis-endemic areas. Parasitol Today. 1996;12:448\u201350.","journal-title":"Parasitol Today"},{"key":"3282_CR6","doi-asserted-by":"publisher","first-page":"804","DOI":"10.4269\/ajtmh.1993.49.804","volume":"49","author":"J Kazura","year":"1993","unstructured":"Kazura J, Greenberg J, Perry R, Weil G, Day K, Alpers M. Comparison of single-dose diethylcarbamazine and ivermectin for treatment of bancroftian filariasis in Papua New Guinea. Am J Trop Med Hyg. 1993;49:804\u201311.","journal-title":"Am J Trop Med Hyg"},{"key":"3282_CR7","doi-asserted-by":"publisher","first-page":"495","DOI":"10.1080\/00034983.2001.11813662","volume":"95","author":"M Boussinesq","year":"2001","unstructured":"Boussinesq M, Gardon J, Kamgno J, Pion S, Gardon-Wendel N, Chippaux J-P. Relationships between the prevalence and intensity of Loa loa infection in the Central Province of Cameroon. Ann Trop Med Parasitol. 2001;95:495\u2013507.","journal-title":"Ann Trop Med Parasitol"},{"key":"3282_CR8","doi-asserted-by":"publisher","first-page":"715","DOI":"10.1179\/136485906X112194","volume":"100","author":"M Boussinesq","year":"2006","unstructured":"Boussinesq M. Loiasis. Ann Trop Med Parasitol. 2006;100:715\u201331.","journal-title":"Ann Trop Med Parasitol"},{"key":"3282_CR9","doi-asserted-by":"publisher","first-page":"529","DOI":"10.4269\/ajtmh.1985.34.529","volume":"34","author":"H Francis","year":"1985","unstructured":"Francis H, Awadzi K, Ottesen EA. The mazzotti reaction following treatment of onchocerciasis with diethylcarbamazine: clinical severity as a function of infection intensity. Am J Trop Med Hyg. 1985;34:529\u201336.","journal-title":"Am J Trop Med Hyg"},{"key":"3282_CR10","doi-asserted-by":"publisher","first-page":"21","DOI":"10.4269\/ajtmh.17-0390","volume":"98","author":"M O\u2019Neill","year":"2018","unstructured":"O\u2019Neill M, Njouendou JA, Dzimianski M, Burkman E, Ndongmo PC, Kengne-Ouafo JA, et al. Potential role for flubendazole in limiting filariasis transmission: observations of microfilarial sensitivity. Am J Trop Med Hyg. 2018;98:21\u20136.","journal-title":"Am J Trop Med Hyg"},{"key":"3282_CR11","doi-asserted-by":"publisher","first-page":"43","DOI":"10.1186\/1756-3305-5-43","volume":"5","author":"N Tendongfor","year":"2012","unstructured":"Tendongfor N, Wanji S, Ngwa JC, Esum ME, Specht S, Enyong P, et al. The human parasite Loa loa in cytokine and cytokine receptor gene knock out BALB\/c mice: survival, development and localization. Parasit Vectors. 2012;5:43.","journal-title":"Parasit Vectors"},{"key":"3282_CR12","doi-asserted-by":"publisher","first-page":"563","DOI":"10.1186\/s13071-017-2515-8","volume":"10","author":"AJ Njouendou","year":"2017","unstructured":"Njouendou AJ, Ritter M, Ndongmo WPC, Kien CA, Narcisse GTV, Fombad FF, et al. Successful long-term maintenance of Mansonella perstans in an in vitro culture system. Parasit Vectors. 2017;10:563.","journal-title":"Parasit Vectors"},{"key":"3282_CR13","doi-asserted-by":"publisher","first-page":"275","DOI":"10.1186\/s13071-018-2852-2","volume":"11","author":"D Zofou","year":"2018","unstructured":"Zofou D, Fombad FF, Gandjui NVT, Njouendou AJ, Kengne-Ouafo AJ, Chounna Ndongmo PW, et al. Evaluation of in vitro culture systems for the maintenance of microfilariae and infective larvae of Loa loa. Parasit Vectors. 2018;11:275.","journal-title":"Parasit Vectors"},{"key":"3282_CR14","doi-asserted-by":"publisher","first-page":"e93072","DOI":"10.1371\/journal.pone.0093072","volume":"9","author":"J Ajendra","year":"2014","unstructured":"Ajendra J, Specht S, Neumann A-L, Gondorf F, Schmidt D, Gentil K, et al. ST2 deficiency does not impair type 2 immune responses during chronic filarial infection but leads to an increased microfilaremia due to an impaired splenic microfilarial clearance. PLoS One. 2014;9:e93072.","journal-title":"PLoS One."},{"key":"3282_CR15","volume-title":"R: A Language and Environment for Statistical Computing. 3.4.1 edn. Vienna","author":"R Development Core Team","year":"2017","unstructured":"Development Core Team R. R: A Language and Environment for Statistical Computing. 3.4.1 edn. Vienna. Austria: Foundation for Statistical Computing; 2017. \n                    http:\/\/www.R-project.org\/"},{"key":"3282_CR16","doi-asserted-by":"publisher","first-page":"497","DOI":"10.1586\/eri.11.30","volume":"9","author":"CD Mackenzie","year":"2011","unstructured":"Mackenzie CD, Geary TG. Flubendazole: a candidate for a field usable macrofilariacide for lymphatic filariasis and onchocerciasis field programs. Expert Rev Anti Infect Ther. 2011;9:497\u2013501.","journal-title":"Expert Rev Anti Infect Ther"},{"key":"3282_CR17","doi-asserted-by":"publisher","first-page":"681","DOI":"10.1586\/eri.11.76","volume":"9","author":"TG Geary","year":"2011","unstructured":"Geary TG, Mackenzie CD. Progress and challenges in the discovery of macrofilaricidal drugs. Expert Rev Anti Infect Ther. 2011;9:681\u201395.","journal-title":"Expert Rev Anti Infect Ther"},{"key":"3282_CR18","doi-asserted-by":"publisher","first-page":"673","DOI":"10.1016\/0035-9203(79)90018-X","volume":"73","author":"DA Denham","year":"1979","unstructured":"Denham DA, Samad R, Cho SY, Suswillo RR, Skippins SC. The anthelmintic effects of flubendazole on Brugia pahangi. Trans R Soc Trop Med Hyg. 1979;73:673\u20136.","journal-title":"Trans R Soc Trop Med Hyg"},{"key":"3282_CR19","doi-asserted-by":"publisher","first-page":"181","DOI":"10.1017\/S0022149X00011494","volume":"62","author":"S Townson","year":"1988","unstructured":"Townson S, Dobinson A, Connelly C, Muller R. Chemotherapy of Onchocerca lienalis microfilariae in mice: a model for the evaluation of novel compounds for the treatment of onchocerciasis. J Helminthol. 1988;62:181\u201394.","journal-title":"J Helminthol"},{"key":"3282_CR20","doi-asserted-by":"publisher","first-page":"223","DOI":"10.1186\/s13071-018-2799-3","volume":"11","author":"AJ Njouendou","year":"2018","unstructured":"Njouendou AJ, Fombad FF, O\u2019Neill M, Zofou D, Nutting C, Ndongmo PC, et al. Heterogeneity in the in vitro susceptibility of Loa loa microfilariae to drugs commonly used in parasitological infections. Parasit Vectors. 2018;11:223.","journal-title":"Parasit Vectors"},{"key":"3282_CR21","doi-asserted-by":"publisher","first-page":"890","DOI":"10.1016\/0035-9203(88)90029-6","volume":"82","author":"I Kerckhoven Van","year":"1988","unstructured":"Van Kerckhoven I, Kumar V. Macrofilaricidal activity of oral flubendazole on Brugia pahangi. Trans R Soc Trop Med Hyg. 1988;82:890\u20131.","journal-title":"Trans R Soc Trop Med Hyg"},{"key":"3282_CR22","doi-asserted-by":"publisher","first-page":"306","DOI":"10.1016\/0035-9203(81)90343-6","volume":"75","author":"JW Mak","year":"1981","unstructured":"Mak JW. Antifilarial activity of mebendazole and flubendazole on Breinlia booliati. Trans R Soc Trop Med Hyg. 1981;75:306\u20137.","journal-title":"Trans R Soc Trop Med Hyg"},{"key":"3282_CR23","doi-asserted-by":"publisher","first-page":"18","DOI":"10.1186\/1756-3305-1-18","volume":"1","author":"B Bronsvoort","year":"2008","unstructured":"Bronsvoort B, Makepeace B, Renz A, Tanya V, Fleckenstein L, Ekale D, et al. UMF-078: a modified flubendazole with potent macrofilaricidal activity against Onchocerca ochengi in African cattle. Parasit Vectors. 2008;1:18.","journal-title":"Parasit Vectors"},{"key":"3282_CR24","doi-asserted-by":"publisher","first-page":"606","DOI":"10.1111\/j.1365-2885.2009.01082.x","volume":"32","author":"V Krizova","year":"2009","unstructured":"Krizova V, Nobilis M, Pruskova L, Chladek J, Szotakova B, Cvilink V, et al. Pharmacokinetics of flubendazole and its metabolites in lambs and adult sheep (Ovis aries). J Vet Pharmacol Ther. 2009;32:606\u201312.","journal-title":"J Vet Pharmacol Ther"},{"key":"3282_CR25","doi-asserted-by":"publisher","first-page":"299","DOI":"10.1111\/j.1365-2885.2004.00616.x","volume":"27","author":"L Moreno","year":"2004","unstructured":"Moreno L, Alvarez L, Mottier L, Virkel G, Bruni SS, Lanusse C. Integrated pharmacological assessment of flubendazole potential for use in sheep: disposition kinetics, liver metabolism and parasite diffusion ability. J Vet Pharmacol Ther. 2004;27:299\u201308.","journal-title":"J Vet Pharmacol Ther"},{"key":"3282_CR26","doi-asserted-by":"publisher","first-page":"354","DOI":"10.1016\/j.parint.2009.07.006","volume":"58","author":"L Ceballos","year":"2009","unstructured":"Ceballos L, Elissondo M, Bruni SS, Denegri G, Alvarez L, Lanusse C. Flubendazole in cystic echinococcosis therapy: pharmaco-parasitological evaluation in mice. Parasitol Int. 2009;58:354\u20138.","journal-title":"Parasitol Int"},{"key":"3282_CR27","doi-asserted-by":"publisher","first-page":"178","DOI":"10.1016\/j.ijpddr.2015.09.001","volume":"5","author":"L Ceballos","year":"2015","unstructured":"Ceballos L, Alvarez L, Mackenzie C, Geary T, Lanusse C. Pharmacokinetic comparison of different flubendazole formulations in pigs: a further contribution to its development as a macrofilaricide molecule. Int J Parasitol Drugs Drug Resist. 2015;5:178\u201384.","journal-title":"Int J Parasitol Drugs Drug Resist"},{"key":"3282_CR28","doi-asserted-by":"publisher","first-page":"e2838","DOI":"10.1371\/journal.pntd.0002838","volume":"8","author":"L Ceballos","year":"2014","unstructured":"Ceballos L, Mackenzie C, Geary T, Alvarez L, Lanusse C. Exploring the potential of flubendazole in filariasis control: evaluation of the systemic exposure for different pharmaceutical preparations. PLoS Negl Trop Dis. 2014;8:e2838.","journal-title":"PLoS Negl Trop Dis"},{"key":"3282_CR29","doi-asserted-by":"publisher","first-page":"5861","DOI":"10.1128\/AAC.05105-11","volume":"55","author":"L Ceballos","year":"2011","unstructured":"Ceballos L, Elissondo C, Bruni SS, Denegri G, Lanusse C, Alvarez L. Comparative performance of flubendazole and albendazole in cystic echinococcosis: Ex vivo activity, plasma\/cysts disposition and efficacy in infected mice. Antimicrob Agents Chemother. 2011;55:5861\u20137.","journal-title":"Antimicrob Agents Chemother"},{"key":"3282_CR30","doi-asserted-by":"publisher","first-page":"211","DOI":"10.1016\/j.vetpar.2009.05.014","volume":"164","author":"L Alvarez","year":"2009","unstructured":"Alvarez L, Moreno G, Moreno L, Ceballos L, Shaw L, Fairweather I, Lanusse C. Comparative assessment of albendazole and triclabendazole ovicidal activity on Fasciola hepatica eggs. Vet Parasitol. 2009;164:211\u20136.","journal-title":"Vet Parasitol"},{"key":"3282_CR31","doi-asserted-by":"publisher","first-page":"295","DOI":"10.1159\/000342924","volume":"58","author":"L Urbizu","year":"2012","unstructured":"Urbizu L, Confalonieri A, Bruni SS, Lanusse C, Alvarez LI. Nematodicidal activity of flubendazole and its reduced metabolite on a murine model of Trichinella spiralis infection. Chemotherapy. 2012;58:295\u20138.","journal-title":"Chemotherapy"},{"key":"3282_CR32","doi-asserted-by":"publisher","first-page":"133","DOI":"10.1016\/j.ijpddr.2014.05.003","volume":"4","author":"AP Page","year":"2014","unstructured":"Page AP, Stepek G, Winter AD, Pertab D. Enzymology of the nematode cuticle: A potential drug target? Int J Parasitol Drugs Drug Resist. 2014;4:133\u201341.","journal-title":"Int J Parasitol Drugs Drug Resist"},{"key":"3282_CR33","doi-asserted-by":"publisher","first-page":"32","DOI":"10.1016\/S0140-6736(86)92571-7","volume":"328","author":"C Hollands","year":"1986","unstructured":"Hollands C. The Animals (Scientific Procedures) Act 1986. Lancet. 1986;328:32\u20133.","journal-title":"Lancet"}],"container-title":["Parasites &amp; Vectors"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/link.springer.com\/content\/pdf\/10.1186\/s13071-018-3282-x.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/link.springer.com\/article\/10.1186\/s13071-018-3282-x\/fulltext.html","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/link.springer.com\/content\/pdf\/10.1186\/s13071-018-3282-x.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2020,1,7]],"date-time":"2020-01-07T19:23:16Z","timestamp":1578424996000},"score":1,"resource":{"primary":{"URL":"https:\/\/parasitesandvectors.biomedcentral.com\/articles\/10.1186\/s13071-018-3282-x"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019,1,8]]},"references-count":33,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2019,12]]}},"alternative-id":["3282"],"URL":"https:\/\/doi.org\/10.1186\/s13071-018-3282-x","relation":{},"ISSN":["1756-3305"],"issn-type":[{"value":"1756-3305","type":"electronic"}],"subject":[],"published":{"date-parts":[[2019,1,8]]},"assertion":[{"value":"10 August 2018","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"27 December 2018","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"8 January 2019","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"The study design and protocols were approved by the National Committee for Health Research on Humans (CNERSH) and the REFOTDE Institutional Animal Ethics Committee (RIAEC), and ethical clearances (2015\/09\/640\/CE\/CNERSH\/SP and 001\/RIAEC\/2015, respectively) were issued. Enrolment into the study was on a strictly voluntary basis and the objectives, risks and benefits of the study were explained in detail to all individuals. We explained in detail the objectives of the study, and any possible health risks, to the parasite donor to ensure that he was well informed and was giving his consent freely and willingly, after which he signed a consent form. Handling of the animals and the investigations carried out were in strict accordance with the international guidelines of rearing animals and using them in medical research. The manipulations of the animals were in strict accordance with the Animal Welfare Legislation and Policies and complied with the Animals (Scientific Procedures) Act 1986 (ASPA) and its associated codes of practice on animal housing and care []. Previous works that used the same procedures are found here [].","order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Ethics approval and consent to participate"}},{"value":"Not applicable.","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Consent for publication"}},{"value":"The authors declare that they have no competing interests.","order":3,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing interests"}},{"value":"Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.","order":4,"name":"Ethics","group":{"name":"EthicsHeading","label":"Publisher\u2019s Note"}}],"article-number":"14"}}