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The aim of this study was to provide epidemiological data regarding the presence of\n                      <jats:italic>Borrelia<\/jats:italic>\n                      spp. in tissues of small mammals from Romania.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      We examined 401 individuals belonging to 11 small mammal species collected in Romania. Collections cover the largest effort to survey these reservoirs in the country. Tissue samples were analyzed by multiplex qPCR targeting the\n                      <jats:italic>ospA<\/jats:italic>\n                      gene of\n                      <jats:italic>Borrelia burgdorferi<\/jats:italic>\n                      (\n                      <jats:italic>s.l.<\/jats:italic>\n                      ) and a part of the\n                      <jats:italic>flaB<\/jats:italic>\n                      gene of\n                      <jats:italic>B. miyamotoi<\/jats:italic>\n                      . Positive samples were further analysed by conventional PCR and sequenced.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      The overall prevalence of infection with\n                      <jats:italic>Borrelia<\/jats:italic>\n                      spp. in small mammal tissues was 4.9%. The most commonly detected species were\n                      <jats:italic>B. afzelii<\/jats:italic>\n                      , followed by\n                      <jats:italic>B. garinii<\/jats:italic>\n                      \/\n                      <jats:italic>B. bavariensis<\/jats:italic>\n                      ,\n                      <jats:italic>B. miyamotoi<\/jats:italic>\n                      and\n                      <jats:italic>B. burgdorferi<\/jats:italic>\n                      (\n                      <jats:italic>s.s<\/jats:italic>\n                      .). To our knowledge, we report for the first time the detection of\n                      <jats:italic>Borrelia<\/jats:italic>\n                      spp. in\n                      <jats:italic>Crocidura leucodon<\/jats:italic>\n                      and\n                      <jats:italic>C<\/jats:italic>\n                      .\n                      <jats:italic>suaveolens<\/jats:italic>\n                      , and\n                      <jats:italic>B<\/jats:italic>\n                      .\n                      <jats:italic>miyamotoi<\/jats:italic>\n                      in the liver of\n                      <jats:italic>Myodes glareolus<\/jats:italic>\n                      .\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>\n                      To our knowledge, our study evaluates for the first time the occurrence of\n                      <jats:italic>Borrelia<\/jats:italic>\n                      spp. in small mammals in Romania, contributing to a better knowledge of the distribution of these bacteria. This survey upgrades previous data on the spatial distribution of the pathogens and reveals the importance of animal surveillance regarding Lyme borreliosis and relapsing fever caused by\n                      <jats:italic>B. miyamotoi<\/jats:italic>\n                      .\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1186\/s13071-019-3713-3","type":"journal-article","created":{"date-parts":[[2019,9,24]],"date-time":"2019-09-24T04:03:09Z","timestamp":1569297789000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":16,"title":["Borrelia spp. in small mammals in Romania"],"prefix":"10.1186","volume":"12","author":[{"given":"Zsuzsa","family":"Kalm\u00e1r","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Attila D\u00e1vid","family":"S\u00e1ndor","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ioana Adriana","family":"Matei","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Angela","family":"Ionic\u0103","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gianluca","family":"D\u2019Amico","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"C\u0103lin Mircea","family":"Gherman","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andrei Daniel","family":"Mihalca","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2019,9,24]]},"reference":[{"key":"3713_CR1","doi-asserted-by":"publisher","first-page":"e107387","DOI":"10.1371\/journal.pone.0107387","volume":"9","author":"RS Ostfeld","year":"2014","unstructured":"Ostfeld RS, Levi T, Jolles AE, Martin LB, Hosseini PR, Keesing F, et al. 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The Research Bioethics Commission of University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca (USAMV CN) committee reviewed and approved the document. The Research Bioethics Commission of USAMV CN approved this study.","order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Ethics approval and consent to participate"}},{"value":"Not applicable.","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Consent for publication"}},{"value":"The authors declare that they have no competing interests.","order":3,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing interests"}}],"article-number":"461"}}