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Recent molecular taxonomic studies conducted in Southeast Asia and Australia, showed that dogs can carry the same genotypes of\n                    <jats:italic>S. stercoralis<\/jats:italic>\n                    that also infect humans, in addition to a presumably dog-specific\n                    <jats:italic>Strongyloides<\/jats:italic>\n                    species. This suggests a potential for zoonotic transmission of\n                    <jats:italic>S. stercoralis<\/jats:italic>\n                    from dogs to humans. Although natural\n                    <jats:italic>S. stercoralis<\/jats:italic>\n                    infections have not been reported in any host other than humans, non-human primates and dogs, other as yet unidentified animal reservoirs cannot be excluded. Molecular studies also showed that humans carry rather different genotypes of\n                    <jats:italic>S. stercoralis<\/jats:italic>\n                    . As a result, their taxonomic status and the question of whether they differ in their pathogenic potential remains open. It would therefore be very important to obtain molecular genetic\/genomic information about\n                    <jats:italic>S. stercoralis<\/jats:italic>\n                    populations from around the world. One way of achieving this (with little additional sampling effort) would be that people encountering\n                    <jats:italic>S. stercoralis<\/jats:italic>\n                    in the process of their diagnostic work preserve some specimens for molecular analysis. Here we provide a guideline for the isolation, preservation, genotyping at the nuclear\n                    <jats:italic>18S<\/jats:italic>\n                    rDNA and the mitochondrial\n                    <jats:italic>cox<\/jats:italic>\n                    1 loci, and for whole genome sequencing of single\n                    <jats:italic>S. stercoralis<\/jats:italic>\n                    worms. Since in many cases the full analysis is not possible or desired at the place and time where\n                    <jats:italic>S. stercoralis<\/jats:italic>\n                    are found, we emphasize when and how samples can be preserved, stored and shipped for later analysis. We hope this will benefit and encourage researchers conducting field studies or diagnostics to collect and preserve\n                    <jats:italic>S. stercoralis<\/jats:italic>\n                    for molecular genetic\/genomic analyses and either analyze them themselves or make them available to others for further analysis.\n                  <\/jats:p>","DOI":"10.1186\/s13071-019-3748-5","type":"journal-article","created":{"date-parts":[[2019,10,23]],"date-time":"2019-10-23T00:28:23Z","timestamp":1571790503000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":29,"title":["From the feces to the genome: a guideline for the isolation and preservation of Strongyloides stercoralis in the field for genetic and genomic analysis of individual worms"],"prefix":"10.1186","volume":"12","author":[{"given":"Siyu","family":"Zhou","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dorothee","family":"Harbecke","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7838-621X","authenticated-orcid":false,"given":"Adrian","family":"Streit","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2019,10,22]]},"reference":[{"key":"3748_CR1","doi-asserted-by":"publisher","first-page":"e0004898","DOI":"10.1371\/journal.pntd.0004898","volume":"10","author":"M Albonico","year":"2016","unstructured":"Albonico M, Becker SL, Odermatt P, Angheben A, Anselmi M, Amor A, et al. 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