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It can transmit many infectious pathogens, including the causative agents of human rickettsiosis, bovine theileriosis, bovine babesiosis and canine babesiosis. Therefore, a greater understanding of<jats:italic>H. longicornis<\/jats:italic>biology might aid in the development of more effective control measures against the tick and tick-borne pathogens.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>We analyzed the expression of miR-184 in different developmental stages and various tissues of<jats:italic>H. longicornis<\/jats:italic>using real-time PCR (qRT-PCR). Antagomir (Ant-184) was used to knock-down miR-184, whilst Ms-Ant and non-injected ticks were used as the negative and blank controls, respectively. We used online software tools (RNAhybrid and TargetScan) to predict the putative target genes of miR-184.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>The expression of miR-184 was highest in unfed nymphs and lowest in unfed larvae. The tissue distribution of miR-184 showed abundant expression in the midgut. To investigate the probable roles of miR-184, antagomir (Ant-184) was used to knock-down miR-184 (<jats:italic>t<\/jats:italic><jats:sub>(4)<\/jats:sub>= 12.32,<jats:italic>P<\/jats:italic>= 0.0002). After inhibiting miR-184, other biological factors were examined in each group. The engorged body weight was significantly reduced in the treated group (Ant-184) in contrast to control groups (<jats:italic>t<\/jats:italic><jats:sub>(22)<\/jats:sub>= 2.19,<jats:italic>P<\/jats:italic>= 0.0388). The mean duration of the egg-laying days was significantly increased (33.5 \u00b1 1.91) and the number of eggs (<jats:italic>t<\/jats:italic><jats:sub>(10)<\/jats:sub>= 3.147,<jats:italic>P<\/jats:italic>= 0.0137), and egg mass (<jats:italic>t<\/jats:italic><jats:sub>(10)<\/jats:sub>= 3.4472,<jats:italic>P<\/jats:italic>= 0.0063) were significantly reduced in the treated group. During oviposition, eggs were monitored and in half of the ticks of the Ant-184 group the eggs were completely desiccated, lacked embryo development and did not hatch. We analyzed the expression of Vg proteins (Vg1, Vg2, Vg3) in semi-engorged ticks, engorged ticks, ticks at day 2 after engorgement and egg stage in Ant-184, non-injected and Ms-Ant groups, and found significant variation.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>This study provides information on the role of miR-184 in<jats:italic>H. longicornis<\/jats:italic>ticks. The data suggest that miR-184 targets Vg proteins and affects blood digestion and oviposition.<\/jats:p><\/jats:sec>","DOI":"10.1186\/s13071-019-3754-7","type":"journal-article","created":{"date-parts":[[2019,10,25]],"date-time":"2019-10-25T18:01:55Z","timestamp":1572026515000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Localized expression and inhibition effect of miR-184 on blood digestion and oviposition in Haemaphysalis longicornis (Acari: Ixodidae)"],"prefix":"10.1186","volume":"12","author":[{"given":"Muhammad Irfan","family":"Malik","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mohsin","family":"Nawaz","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yanan","family":"Wang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Houshuang","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jie","family":"Cao","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yongzhi","family":"Zhou","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ibrahim A.","family":"Hassan","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Md. Nazrul","family":"Islam","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Muhammad Naveed","family":"Anwar","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3383-1084","authenticated-orcid":false,"given":"Jinlin","family":"Zhou","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2019,10,25]]},"reference":[{"key":"3754_CR1","doi-asserted-by":"publisher","first-page":"74","DOI":"10.1016\/S1471-4922(00)01856-0","volume":"17","author":"SE Shaw","year":"2001","unstructured":"Shaw SE, Day MJ, Birtles RJ, Breitschwerdt EB. Tick-borne infectious diseases of dogs. 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