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Marking insects has been routinely carried out for over 100\u00a0years; however, there is no gold standard regarding the marking of specific disease-transmitting mosquitoes including\n                      <jats:italic>Anopheles arabiensis<\/jats:italic>\n                      ,\n                      <jats:italic>Aedes aegypti<\/jats:italic>\n                      and\n                      <jats:italic>Aedes albopictus<\/jats:italic>\n                      . The research presented offers a novel dusting technique and optimal dust colour and quantities, suitable for small-scale releases, such as mark-release-recapture studies.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      We sought to establish a suitable dust colour and quantity for batches of 100 male\n                      <jats:italic>An. arabiensis<\/jats:italic>\n                      , that was visible both by eye and under UV light, long-lasting and did not negatively impact longevity. A set of lower dust weights were selected to conduct longevity experiments with both\n                      <jats:italic>Ae. aegypti<\/jats:italic>\n                      and\n                      <jats:italic>Ae. albopictus<\/jats:italic>\n                      to underpin the optimal dust weight. A further study assessed the potential of marked male\n                      <jats:italic>An. arabiensis<\/jats:italic>\n                      to transfer their mark to undusted males and females.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      The longevity of male\n                      <jats:italic>An. arabiensis<\/jats:italic>\n                      marked with various dust colours was not significantly reduced when compared to unmarked controls. Furthermore, the chosen dust quantity (5\u00a0mg) did not negatively impact longevity (\n                      <jats:italic>P<\/jats:italic>\n                      \u2009=\u20090.717) and provided a long-lasting mark. Dust transfer was found to occur from marked\n                      <jats:italic>An. arabiensis<\/jats:italic>\n                      males to unmarked males and females when left in close proximity. However, this was only noticeable when examining individuals under a stereomicroscope and thus deemed negligible. Overall, male\n                      <jats:italic>Ae. aegypti<\/jats:italic>\n                      and\n                      <jats:italic>Ae. albopictus<\/jats:italic>\n                      displayed a greater sensitivity to dusting. Only the lowest dust weight (0.5\u00a0mg) did not significantly reduce longevity (\n                      <jats:italic>P<\/jats:italic>\n                      \u2009=\u20090.888) in\n                      <jats:italic>Ae. aegypti<\/jats:italic>\n                      , whilst the lowest two dust weights (0.5 and 0.75\u00a0mg) had no significant impact on longevity (\n                      <jats:italic>P<\/jats:italic>\n                      \u2009=\u20090.951 and 0.166, respectively) in\n                      <jats:italic>Ae. albopictus<\/jats:italic>\n                      .\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>We have devised a fast, inexpensive and simple marking method and provided recommended dust quantities for several major species of disease-causing mosquitoes. The novel technique provides an evenly distributed, long-lasting mark which is non-detrimental. Our results will be useful for future MRR studies, prior to a major release campaign.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1186\/s13071-020-04066-6","type":"journal-article","created":{"date-parts":[[2020,4,15]],"date-time":"2020-04-15T07:03:17Z","timestamp":1586934197000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":19,"title":["A standardised method of marking male mosquitoes with fluorescent dust"],"prefix":"10.1186","volume":"13","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1059-4733","authenticated-orcid":false,"given":"Nicole J.","family":"Culbert","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria","family":"Kaiser","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nelius","family":"Venter","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marc J. 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