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For a preventive strategy against infectious diseases, vaccination is the most effective. Here, we evaluated the molecular characteristics and immune responses of CsAg17 protein from<jats:italic>Clonorchis sinensis<\/jats:italic>, and investigated its protective effects against<jats:italic>C. sinensis<\/jats:italic>challenge.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>A cDNA clone encoding CsAg17 protein and containing a secretory signal peptide at the N-terminus was retrieved from the<jats:italic>C. sinensis<\/jats:italic>transcriptome bank. Recombinant CsAg17 B-cell epitope protein and cDNA vaccines were produced and their immune responses were evaluated in FVB mice. The proportional changes of CD3<jats:sup>+<\/jats:sup>\/CD4<jats:sup>+<\/jats:sup>and CD3<jats:sup>+<\/jats:sup>\/CD8<jats:sup>+<\/jats:sup>T cells were detected by flow cytometry, and immune effectors were measured by ELISA.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>The<jats:italic>CsAg17<\/jats:italic>mRNA was transcribed at a higher level in<jats:italic>C. sinensis<\/jats:italic>adults than in metacercariae. The CsAg17 protein was distributed in the sperms, oral and ventral suckers, and mesenchymal tissues of<jats:italic>C. sinensis<\/jats:italic>adults. In mice challenged with<jats:italic>C. sinensis<\/jats:italic>metacercariae, vaccination with CsAg17 protein and cDNA resulted in a reduction to 64% and 69% in worm burden, respectively. Both CsAg17 protein and cDNA vaccines increased the proportion of CD3<jats:sup>+<\/jats:sup>\/CD4<jats:sup>+<\/jats:sup>and CD3<jats:sup>+<\/jats:sup>\/CD8<jats:sup>+<\/jats:sup>T cells and stimulated the production of Th1 type cytokines such as interleukin (IL)-2, IL-12, and interferon-\u03b3, while maintaining minimum levels of Th2 cytokines. The levels of IgG specific to CsAg17 protein steeply increased in the two vaccinated groups from 2 weeks after immunization. The liver tissue retained good morphology in the mice vaccinated with CsAg17 protein or cDNA, whereas severe inflammation and large serous cysts were observed in the liver of the unvaccinated mice.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Vaccination with CsAg17 protein and cDNA reduced the pathological changes in the bile duct and liver, and ameliorated the worm burden<jats:italic>via<\/jats:italic>cellular and humoral immune responses. Thus, they may serve as good vaccine candidates against<jats:italic>C. sinensis<\/jats:italic>infections.<\/jats:p><\/jats:sec>","DOI":"10.1186\/s13071-020-04083-5","type":"journal-article","created":{"date-parts":[[2020,4,25]],"date-time":"2020-04-25T18:03:33Z","timestamp":1587837813000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["Clonorchis sinensis secretory protein CsAg17 vaccine induces immune protection"],"prefix":"10.1186","volume":"13","author":[{"given":"Xuelian","family":"Bai","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jin-Ho","family":"Song","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fuhong","family":"Dai","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ji-Yun","family":"Lee","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sung-Jong","family":"Hong","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2020,4,25]]},"reference":[{"key":"4083_CR1","doi-asserted-by":"publisher","first-page":"31","DOI":"10.1016\/S1473-3099(04)01252-6","volume":"5","author":"ZR Lun","year":"2005","unstructured":"Lun ZR, Gasser RB, Lai DH, Li AX, Zhu XQ, Yu XB, et al. 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Approval for animal experiments was obtained from the Institutional Review Board of Chung-Ang University animal facility (Approval Number: CAU-2011-0052 and CAU-2011-0053).","order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Ethics approval and consent to participate"}},{"value":"Not applicable.","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Consent for publication"}},{"value":"The authors declare that they have no competing interests.","order":3,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing interests"}}],"article-number":"215"}}