{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T02:40:02Z","timestamp":1787020802075,"version":"build-2736575974"},"reference-count":42,"publisher":"Springer Science and Business Media LLC","issue":"1","license":[{"start":{"date-parts":[[2020,5,13]],"date-time":"2020-05-13T00:00:00Z","timestamp":1589328000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"},{"start":{"date-parts":[[2020,5,13]],"date-time":"2020-05-13T00:00:00Z","timestamp":1589328000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"}],"funder":[{"DOI":"10.13039\/501100004189","name":"Max-Planck-Gesellschaft","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100004189","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100012309","name":"Royal Golden Jubilee (RGJ) Ph.D. Programme","doi-asserted-by":"publisher","award":["PHD\/0094\/2559"],"award-info":[{"award-number":["PHD\/0094\/2559"]}],"id":[{"id":"10.13039\/501100012309","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004396","name":"Thailand Research Fund","doi-asserted-by":"publisher","award":["DPG6280002"],"award-info":[{"award-number":["DPG6280002"]}],"id":[{"id":"10.13039\/501100004396","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Parasites Vectors"],"published-print":{"date-parts":[[2020,12]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>\n                      Strongyloidiasis is a soil borne helminthiasis, which in most cases is caused by\n                      <jats:italic>Strongyloides stercoralis.<\/jats:italic>\n                      Human infections with\n                      <jats:italic>S. fuelleborni fuelleborni<\/jats:italic>\n                      and\n                      <jats:italic>S. fuelleborni kellyi<\/jats:italic>\n                      also occur. Although up to 370 million people are currently estimated to be infected with\n                      <jats:italic>S. stercoralis<\/jats:italic>\n                      , this parasite is frequently overlooked.\n                      <jats:italic>Strongyloides stercoralis<\/jats:italic>\n                      is prevalent among humans in Thailand; however,\n                      <jats:italic>S. fuelleborni fuelleborni<\/jats:italic>\n                      has also been reported. Three recent genomic studies of individual\n                      <jats:italic>S. stercoralis<\/jats:italic>\n                      worms found genetically diverse populations of\n                      <jats:italic>S. stercoralis<\/jats:italic>\n                      , with comparably low heterozygosity in Cambodia and Myanmar, and less diverse populations with high heterozygosity in Japan and southern China that presumably reproduce asexually.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      We isolated individual\n                      <jats:italic>Strongyloides<\/jats:italic>\n                      spp. from different localities in northern and western Thailand and determined their nuclear small ribosomal subunit rDNA (\n                      <jats:italic>18S<\/jats:italic>\n                      rDNA,\n                      <jats:italic>SSU<\/jats:italic>\n                      ), in particular the hypervariable regions I and IV (HVR-I and HVR-IV), mitochondrial cytochrome\n                      <jats:italic>c<\/jats:italic>\n                      oxidase subunit 1 (\n                      <jats:italic>cox<\/jats:italic>\n                      1) and for a subset whole genome sequences. These sequences were then compared with each other and with published sequences from different geographical locations.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      All 237 worms isolated from 16 different human hosts were\n                      <jats:italic>S. stercoralis<\/jats:italic>\n                      , no\n                      <jats:italic>S. fuelleborni<\/jats:italic>\n                      was found. All worms had the common\n                      <jats:italic>S. stercoralis SSU<\/jats:italic>\n                      HVR IV haplotype A. Two different\n                      <jats:italic>SSU<\/jats:italic>\n                      HVR I haplotypes (I and II), both previously described in\n                      <jats:italic>S. stercoralis<\/jats:italic>\n                      , were found. No animal heterozygous for the two haplotypes was identified. Among the twelve\n                      <jats:italic>cox<\/jats:italic>\n                      1 haplotypes found, five had not been previously described. Based upon the mitochondrial\n                      <jats:italic>cox<\/jats:italic>\n                      1 and the nuclear whole genome sequences,\n                      <jats:italic>S. stercoralis<\/jats:italic>\n                      in Thailand was phylogenetically intermixed with the samples from other Southeast Asian countries and did not form its own branch. The genomic heterozygosity was even slightly lower than in the samples from the neighboring countries.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>\n                      In our sample from humans, all\n                      <jats:italic>Strongyloides<\/jats:italic>\n                      spp. were\n                      <jats:italic>S. stercoralis<\/jats:italic>\n                      . The\n                      <jats:italic>S. stercoralis<\/jats:italic>\n                      from northern and western Thailand appear to be part of a diverse, intermixing continental Southeast Asian population. No obvious indication for genetic sub-structuring of\n                      <jats:italic>S. stercoralis<\/jats:italic>\n                      within Thailand or within the Southeast Asian peninsula was detected.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1186\/s13071-020-04115-0","type":"journal-article","created":{"date-parts":[[2020,5,13]],"date-time":"2020-05-13T09:05:53Z","timestamp":1589360753000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":17,"title":["Genomic studies on Strongyloides stercoralis in northern and western Thailand"],"prefix":"10.1186","volume":"13","author":[{"given":"Kittipat","family":"Aupalee","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Adulsak","family":"Wijit","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kittikhun","family":"Singphai","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christian","family":"R\u00f6delsperger","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Siyu","family":"Zhou","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Atiporn","family":"Saeung","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7838-621X","authenticated-orcid":false,"given":"Adrian","family":"Streit","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2020,5,13]]},"reference":[{"key":"4115_CR1","doi-asserted-by":"publisher","first-page":"263","DOI":"10.1017\/S0031182016000834","volume":"144","author":"TB Nutman","year":"2017","unstructured":"Nutman TB. Human infection with Strongyloides stercoralis and other related Strongyloides species. Parasitology. 2017;144:263\u201373.","journal-title":"Parasitology."},{"key":"4115_CR2","doi-asserted-by":"publisher","first-page":"e0004898","DOI":"10.1371\/journal.pntd.0004898","volume":"10","author":"M Albonico","year":"2016","unstructured":"Albonico M, Becker SL, Odermatt P, Angheben A, Anselmi M, Amor A, et al. StrongNet: an international network to improve diagnostics and access to treatment for strongyloidiasis control. PLoS Negl Trop Dis. 2016;10:e0004898.","journal-title":"PLoS Negl Trop Dis."},{"key":"4115_CR3","doi-asserted-by":"publisher","first-page":"e2214","DOI":"10.1371\/journal.pntd.0002214","volume":"7","author":"Z Bisoffi","year":"2013","unstructured":"Bisoffi Z, Buonfrate D, Montresor A, Requena-Mendez A, Munoz J, Krolewiecki AJ, et al. Strongyloides stercoralis: a plea for action. PLoS Negl Trop Dis. 2013;7:e2214.","journal-title":"PLoS Negl Trop Dis."},{"key":"4115_CR4","doi-asserted-by":"publisher","first-page":"967","DOI":"10.1016\/j.trstmh.2009.02.013","volume":"103","author":"A Olsen","year":"2009","unstructured":"Olsen A, van Lieshout L, Marti H, Polderman T, Polman K, Steinmann P, et al. Strongyloidiasis - the most neglected of the neglected tropical diseases? Trans R Soc Trop Med Hyg. 2009;103:967\u201372.","journal-title":"Trans R Soc Trop Med Hyg."},{"key":"4115_CR5","doi-asserted-by":"publisher","first-page":"1521","DOI":"10.1016\/S0140-6736(06)68653-4","volume":"367","author":"J Bethony","year":"2006","unstructured":"Bethony J, Brooker S, Albonico M, Geiger SM, Loukas A, Diemert D, et al. Soil-transmitted helminth infections: ascariasis, trichuriasis, and hookworm. Lancet. 2006;367:1521\u201332.","journal-title":"Lancet."},{"key":"4115_CR6","first-page":"40","volume":"45","author":"W Page","year":"2016","unstructured":"Page W, Speare R. Chronic strongyloidiasis\u2014don\u2019t look and you won\u2019t find. Aust Fam Physician. 2016;45:40\u20134.","journal-title":"Aust Fam Physician."},{"key":"4115_CR7","doi-asserted-by":"publisher","first-page":"227","DOI":"10.1016\/j.actatropica.2015.03.008","volume":"159","author":"F Sch\u00e4r","year":"2016","unstructured":"Sch\u00e4r F, Giardina F, Khieu V, Muth S, Vounatsou P, Marti H, et al. Occurrence of and risk factors for Strongyloides stercoralis infection in South-East Asia. Acta Trop. 2016;159:227\u201338.","journal-title":"Acta Trop."},{"key":"4115_CR8","doi-asserted-by":"publisher","first-page":"89","DOI":"10.1016\/j.actatropica.2012.12.012","volume":"126","author":"F Sch\u00e4r","year":"2013","unstructured":"Sch\u00e4r F, Odermatt P, Khieu V, Panning M, Duong S, Muth S, et al. Evaluation of real-time PCR for Strongyloides stercoralis and hookworm as diagnostic tool in asymptomatic schoolchildren in Cambodia. Acta Trop. 2013;126:89\u201392.","journal-title":"Acta Trop."},{"key":"4115_CR9","doi-asserted-by":"publisher","first-page":"95","DOI":"10.1007\/BF00017661","volume":"18","author":"ME Viney","year":"1991","unstructured":"Viney ME, Ashford RW, Barnish G. A taxonomic study of Strongyloides Grassi, 1879 (Nematoda) with special reference to Strongyloides fuelleborni von Linstow, 1905 in man in Papua New Guinea and the description of a new subspecies. Syst Parasitol. 1991;18:95\u2013109.","journal-title":"Syst Parasitol."},{"key":"4115_CR10","doi-asserted-by":"publisher","first-page":"1507","DOI":"10.1016\/S0020-7519(02)00156-X","volume":"32","author":"M Dorris","year":"2002","unstructured":"Dorris M, Viney ME, Blaxter ML. Molecular phylogenetic analysis of the genus Strongyloides and related nematodes. Int J Parasitol. 2002;32:1507\u201317.","journal-title":"Int J Parasitol."},{"key":"4115_CR11","doi-asserted-by":"publisher","first-page":"255","DOI":"10.1007\/s00436-018-6173-3","volume":"118","author":"W Basso","year":"2019","unstructured":"Basso W, Grandt LM, Magnenat AL, Gottstein B, Campos M. Strongyloides stercoralis infection in imported and local dogs in Switzerland: from clinics to molecular genetics. Parasitol Res. 2019;118:255\u201366.","journal-title":"Parasitol Res."},{"key":"4115_CR12","doi-asserted-by":"publisher","first-page":"e0007241","DOI":"10.1371\/journal.pntd.0007241","volume":"13","author":"M Beknazarova","year":"2019","unstructured":"Beknazarova M, Barratt JLN, Bradbury RS, Lane M, Whiley H, Ross K. Detection of classic and cryptic Strongyloides genotypes by deep amplicon sequencing: a preliminary survey of dog and human specimens collected from remote Australian communities. PLoS Negl Trop Dis. 2019;13:e0007241.","journal-title":"PLoS Negl Trop Dis."},{"key":"4115_CR13","doi-asserted-by":"publisher","first-page":"869","DOI":"10.1007\/s00436-008-1269-9","volume":"104","author":"H Hasegawa","year":"2009","unstructured":"Hasegawa H, Hayashida S, Ikeda Y, Sato H. Hyper-variable regions in 18S rDNA of Strongyloides spp. as markers for species-specific diagnosis. Parasitol Res. 2009;104:869\u201374.","journal-title":"Parasitol Res."},{"key":"4115_CR14","doi-asserted-by":"publisher","first-page":"367","DOI":"10.1016\/j.parint.2016.05.004","volume":"65","author":"H Hasegawa","year":"2016","unstructured":"Hasegawa H, Kalousova B, McLennan MR, Modry D, Profousova-Psenkova I, Shutt-Phillips KA, et al. Strongyloides infections of humans and great apes in Dzanga-Sangha Protected Areas, Central African Republic and in degraded forest fragments in Bulindi. Uganda. Parasitol Int. 2016;65:367\u201370.","journal-title":"Uganda. Parasitol Int."},{"key":"4115_CR15","doi-asserted-by":"publisher","first-page":"407","DOI":"10.1016\/j.parint.2010.05.007","volume":"59","author":"H Hasegawa","year":"2010","unstructured":"Hasegawa H, Sato H, Fujita S, Nguema PP, Nobusue K, Miyagi K, et al. Molecular identification of the causative agent of human strongyloidiasis acquired in Tanzania: dispersal and diversity of Strongyloides spp. and their hosts. Parasitol Int. 2010;59:407\u201313.","journal-title":"Parasitol Int."},{"key":"4115_CR16","doi-asserted-by":"publisher","first-page":"e0005752","DOI":"10.1371\/journal.pntd.0005752","volume":"11","author":"TG Jaleta","year":"2017","unstructured":"Jaleta TG, Zhou S, Bemm FM, Schar F, Khieu V, Muth S, et al. Different but overlapping populations of Strongyloides stercoralis in dogs and humans\u2014dogs as a possible source for zoonotic strongyloidiasis. PLoS Negl Trop Dis. 2017;11:e0005752.","journal-title":"PLoS Negl Trop Dis."},{"key":"4115_CR17","doi-asserted-by":"publisher","first-page":"2973","DOI":"10.1007\/s00436-016-5052-z","volume":"115","author":"S Laymanivong","year":"2016","unstructured":"Laymanivong S, Hangvanthong B, Insisiengmay B, Vanisaveth V, Laxachack P, Jongthawin J, et al. First molecular identification and report of genetic diversity of Strongyloides stercoralis, a current major soil-transmitted helminth in humans from Lao People\u2019s Democratic Republic. Parasitol Res. 2016;115:2973\u201380.","journal-title":"Parasitol Res."},{"key":"4115_CR18","doi-asserted-by":"publisher","first-page":"4844","DOI":"10.1038\/s41598-017-05049-x","volume":"7","author":"E Nagayasu","year":"2017","unstructured":"Nagayasu E, Aung M, Hortiwakul T, Hino A, Tanaka T, Higashiarakawa M, et al. A possible origin population of pathogenic intestinal nematodes, Strongyloides stercoralis, unveiled by molecular phylogeny. Sci Rep. 2017;7:4844.","journal-title":"Sci Rep."},{"key":"4115_CR19","doi-asserted-by":"publisher","first-page":"1917","DOI":"10.1007\/s00436-017-5469-z","volume":"116","author":"T Thanchomnang","year":"2017","unstructured":"Thanchomnang T, Intapan PM, Sanpool O, Rodpai R, Tourtip S, Yahom S, et al. First molecular identification and genetic diversity of Strongyloides stercoralis and Strongyloides fuelleborni in human communities having contact with long-tailed macaques in Thailand. Parasitol Res. 2017;116:1917\u201323.","journal-title":"Parasitol Res."},{"key":"4115_CR20","doi-asserted-by":"publisher","first-page":"e0007396","DOI":"10.1371\/journal.pntd.0007396","volume":"13","author":"S Zhou","year":"2019","unstructured":"Zhou S, Fu X, Pei P, Kucka M, Liu J, Tang L, et al. Characterization of a non-sexual population of Strongyloides stercoralis with hybrid 18S rDNA haplotypes in Guangxi, southern China. PLoS Negl Trop Dis. 2019;13:e0007396.","journal-title":"PLoS Negl Trop Dis."},{"key":"4115_CR21","doi-asserted-by":"publisher","first-page":"496","DOI":"10.1186\/s13071-019-3748-5","volume":"12","author":"S Zhou","year":"2019","unstructured":"Zhou S, Harbecke D, Streit A. From the feces to the genome: a guideline for the isolation and preservation of Strongyloides stercoralis in the field for genetic and genomic analysis of individual worms. Parasit Vectors. 2019;12:496.","journal-title":"Parasit Vectors."},{"key":"4115_CR22","doi-asserted-by":"publisher","first-page":"e0007609","DOI":"10.1371\/journal.pntd.0007609","volume":"13","author":"JLN Barratt","year":"2019","unstructured":"Barratt JLN, Lane M, Talundzic E, Richins T, Robertson G, Formenti F, et al. A global genotyping survey of Strongyloides stercoralis and Strongyloides fuelleborni using deep amplicon sequencing. PLoS Negl Trop Dis. 2019;13:e0007609.","journal-title":"PLoS Negl Trop Dis."},{"key":"4115_CR23","doi-asserted-by":"publisher","first-page":"e0005253","DOI":"10.1371\/journal.pntd.0005253","volume":"10","author":"T Kikuchi","year":"2016","unstructured":"Kikuchi T, Hino A, Tanaka T, Aung MP, Afrin T, Nagayasu E, et al. Genome-wide analyses of individual Strongyloides stercoralis (Nematoda: Rhabditoidea) provide insights into population structure and reproductive life cycles. PLoS Negl Trop Dis. 2016;10:e0005253.","journal-title":"PLoS Negl Trop Dis."},{"key":"4115_CR24","doi-asserted-by":"publisher","first-page":"533","DOI":"10.1016\/j.parint.2014.01.010","volume":"63","author":"F Sch\u00e4r","year":"2014","unstructured":"Sch\u00e4r F, Guo L, Streit A, Khieu V, Muth S, Marti H, et al. Strongyloides stercoralis genotypes in humans in Cambodia. Parasitol Int. 2014;63:533\u20136.","journal-title":"Parasitol Int."},{"key":"4115_CR25","first-page":"442","volume":"36","author":"P Nontasut","year":"2005","unstructured":"Nontasut P, Muennoo C, Sa-nguankiat S, Fongsri S, Vichit A. Prevalence of Strongyloides in northern Thailand and treatment with ivermectin vs albendazole. Southeast Asian J Trop Med Public Health. 2005;36:442\u20134.","journal-title":"Southeast Asian J Trop Med Public Health."},{"key":"4115_CR26","unstructured":"Wijit A, Saeung A. Prevalence of the liver fluke, Opisthorchis viverrini, and minute intestinal flukes based on human copro-DNA diagnosis in the upper northern region of Thailand. In: Final Report: Institute of Research, Knowledge Management and Standards for Disease Control; 2017. p. 49."},{"key":"4115_CR27","doi-asserted-by":"publisher","first-page":"137","DOI":"10.1179\/2047773214Y.0000000134","volume":"108","author":"U Jongwutiwes","year":"2014","unstructured":"Jongwutiwes U, Waywa D, Silpasakorn S, Wanachiwanawin D, Suputtamongkol Y. Prevalence and risk factors of acquiring Strongyloides stercoralis infection among patients attending a tertiary hospital in Thailand. Pathog Glob Health. 2014;108:137\u201340.","journal-title":"Pathog Glob Health."},{"key":"4115_CR28","doi-asserted-by":"publisher","first-page":"940","DOI":"10.1186\/s12889-018-5871-1","volume":"18","author":"P Laoraksawong","year":"2018","unstructured":"Laoraksawong P, Sanpool O, Rodpai R, Thanchomnang T, Kanarkard W, Maleewong W, et al. Current high prevalences of Strongyloides stercoralis and Opisthorchis viverrini infections in rural communities in northeast Thailand and associated risk factors. BMC Pub Health. 2018;18:940.","journal-title":"BMC Pub Health."},{"key":"4115_CR29","doi-asserted-by":"publisher","first-page":"627","DOI":"10.1186\/s12879-017-2723-z","volume":"17","author":"TK Prasongdee","year":"2017","unstructured":"Prasongdee TK, Laoraksawong P, Kanarkard W, Kraiklang R, Sathapornworachai K, Naonongwai S, et al. An eleven-year retrospective hospital-based study of epidemiological data regarding human strongyloidiasis in northeast Thailand. BMC Infect Dis. 2017;17:627.","journal-title":"BMC Infect Dis."},{"key":"4115_CR30","doi-asserted-by":"publisher","first-page":"564","DOI":"10.1186\/s12889-017-4486-2","volume":"17","author":"C Punsawad","year":"2017","unstructured":"Punsawad C, Phasuk N, Bunratsami S, Thongtup K, Siripakonuaong N, Nongnaul S. Prevalence of intestinal parasitic infection and associated risk factors among village health volunteers in rural communities of southern Thailand. BMC Pub Health. 2017;17:564.","journal-title":"BMC Pub Health."},{"key":"4115_CR31","doi-asserted-by":"crossref","unstructured":"Wongsaroj T, Nithikathkul C, Rojkitikul W, Nakai W, Royal L, Rammasut P. Brief communication (Original). National survey of helminthiasis in Thailand. Asian Biomed. 2014;8:779\u2013783.","DOI":"10.5372\/1905-7415.0806.357"},{"key":"4115_CR32","doi-asserted-by":"publisher","first-page":"e110","DOI":"10.1017\/S0022149X1900107X","volume":"94","author":"O Sanpool","year":"2019","unstructured":"Sanpool O, Intapan PM, Rodpai R, Laoraksawong P, Sadaow L, Tourtip S, et al. Dogs are reservoir hosts for possible transmission of human strongyloidiasis in Thailand: molecular identification and genetic diversity of causative parasite species. J Helminthol. 2019;94:e110.","journal-title":"J Helminthol."},{"key":"4115_CR33","doi-asserted-by":"publisher","first-page":"608","DOI":"10.1017\/S0022149X18000512","volume":"93","author":"T Thanchomnang","year":"2019","unstructured":"Thanchomnang T, Intapan PM, Sanpool O, Rodpai R, Sadaow L, Phosuk I, et al. First molecular identification of Strongyloides fuelleborni in long-tailed macaques in Thailand and Lao People\u2019s Democratic Republic reveals considerable genetic diversity. J Helminthol. 2019;93:608\u201315.","journal-title":"J Helminthol."},{"key":"4115_CR34","first-page":"397","volume":"14","author":"N Katz","year":"1972","unstructured":"Katz N, Chaves A, Pellegrino J. A simple device for quantitative stool thick-smear technique in schistosomiasis mansoni. Rev Inst Med Trop Sao Paulo. 1972;14:397\u2013400.","journal-title":"Rev Inst Med Trop Sao Paulo."},{"key":"4115_CR35","doi-asserted-by":"publisher","first-page":"1040","DOI":"10.1086\/322707","volume":"33","author":"AA Siddiqui","year":"2001","unstructured":"Siddiqui AA, Berk SL. Diagnosis of Strongyloides stercoralis infection. Clin Infect Dis. 2001;33:1040\u20137.","journal-title":"Clin Infect Dis."},{"key":"4115_CR36","doi-asserted-by":"publisher","first-page":"1870","DOI":"10.1093\/molbev\/msw054","volume":"33","author":"S Kumar","year":"2016","unstructured":"Kumar S, Stecher G, Tamura K. MEGA7: Molecular Evolutionary Genetics Analysis version 70 for bigger datasets. Mol Biol Evol. 2016;33:1870\u20134.","journal-title":"Mol Biol Evol."},{"key":"4115_CR37","doi-asserted-by":"publisher","first-page":"589","DOI":"10.1093\/bioinformatics\/btp698","volume":"26","author":"H Li","year":"2010","unstructured":"Li H, Durbin R. Fast and accurate long-read alignment with Burrows-Wheeler transform. Bioinformatics. 2010;26:589\u201395.","journal-title":"Bioinformatics."},{"key":"4115_CR38","doi-asserted-by":"publisher","first-page":"1153","DOI":"10.1534\/genetics.113.159855","volume":"196","author":"C R\u00f6delsperger","year":"2014","unstructured":"R\u00f6delsperger C, Neher RA, Weller AM, Eberhardt G, Witte H, Mayer WE, et al. Characterization of genetic diversity in the nematode Pristionchus pacificus from population-scale resequencing data. Genetics. 2014;196:1153\u201365.","journal-title":"Genetics."},{"key":"4115_CR39","doi-asserted-by":"publisher","first-page":"2078","DOI":"10.1093\/bioinformatics\/btp352","volume":"25","author":"H Li","year":"2009","unstructured":"Li H, Handsaker B, Wysoker A, Fennell T, Ruan J, Homer N, et al. The sequence alignment\/map format and SAMtools. Bioinformatics. 2009;25:2078\u20139.","journal-title":"Bioinformatics."},{"key":"4115_CR40","doi-asserted-by":"publisher","first-page":"592","DOI":"10.1093\/bioinformatics\/btq706","volume":"27","author":"KP Schliep","year":"2011","unstructured":"Schliep KP. phangorn: phylogenetic analysis in R. Bioinformatics. 2011;27:592\u20133.","journal-title":"Bioinformatics."},{"key":"4115_CR41","doi-asserted-by":"publisher","first-page":"834","DOI":"10.1016\/j.celrep.2017.09.077","volume":"21","author":"C R\u00f6delsperger","year":"2017","unstructured":"R\u00f6delsperger C, Meyer JM, Prabh N, Lanz C, Bemm F, Sommer RJ. Single-molecule sequencing reveals the chromosome-scale genomic architecture of the nematode model organism Pristionchus pacificus. Cell Rep. 2017;21:834\u201344.","journal-title":"Cell Rep."},{"key":"4115_CR42","doi-asserted-by":"publisher","first-page":"299","DOI":"10.1038\/ng.3495","volume":"48","author":"VL Hunt","year":"2016","unstructured":"Hunt VL, Tsai IJ, Coghlan A, Reid AJ, Holroyd N, Foth BJ, et al. The genomic basis of parasitism in the Strongyloides clade of nematodes. Nat Genet. 2016;48:299\u2013307.","journal-title":"Nat Genet."}],"container-title":["Parasites &amp; Vectors"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1186\/s13071-020-04115-0.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/article\/10.1186\/s13071-020-04115-0\/fulltext.html","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1186\/s13071-020-04115-0.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,5,12]],"date-time":"2021-05-12T19:33:58Z","timestamp":1620848038000},"score":1,"resource":{"primary":{"URL":"https:\/\/parasitesandvectors.biomedcentral.com\/articles\/10.1186\/s13071-020-04115-0"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,5,13]]},"references-count":42,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2020,12]]}},"alternative-id":["4115"],"URL":"https:\/\/doi.org\/10.1186\/s13071-020-04115-0","relation":{},"ISSN":["1756-3305"],"issn-type":[{"value":"1756-3305","type":"electronic"}],"subject":[],"published":{"date-parts":[[2020,5,13]]},"assertion":[{"value":"21 January 2020","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"29 April 2020","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"13 May 2020","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"The protocol for <i>S<\/i>. <i>stercoralis<\/i> collection from participants was conducted with the approval of the research ethics committee of Faculty of Medicine, Chiang Mai University (No. exemption-6815\/2019).","order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Ethics approval and consent to participate"}},{"value":"Not applicable.","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Consent for publication"}},{"value":"The authors declare that they have no competing interests.","order":3,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing interests"}}],"article-number":"250"}}