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In-feed chemoprophylaxis remains the primary method of control, but the increasing ineffectiveness of anticoccidial drugs, and potential future restrictions on their use has encouraged the use of commercial live vaccines. Availability of such formulations is constrained by their production, which relies on the use of live chickens. Several experimental approaches have been taken to explore ways to reduce the complexity and cost of current anticoccidial vaccines including the use of live vectors expressing relevant<jats:italic>Eimeria<\/jats:italic>proteins. We and others have shown that vaccination with transgenic<jats:italic>Eimeria tenella<\/jats:italic>parasites expressing<jats:italic>Eimeria maxima<\/jats:italic>Apical Membrane Antigen-1 or Immune Mapped Protein-1 (<jats:italic>Em<\/jats:italic>AMA1 and<jats:italic>Em<\/jats:italic>IMP1) partially reduces parasite replication after challenge with a low dose of<jats:italic>E. maxima<\/jats:italic>oocysts. In the present study, we have reassessed the efficacy of these experimental vaccines using commercial birds reared at high stocking densities and challenged with both low and high doses of<jats:italic>E. maxima<\/jats:italic>to evaluate how well they protect chickens against the negative impacts of disease on production parameters.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Populations of<jats:italic>E. tenella<\/jats:italic>parasites expressing<jats:italic>Em<\/jats:italic>AMA1 and<jats:italic>Em<\/jats:italic>IMP1 were obtained by nucleofection and propagated in chickens. Cobb500 broilers were immunised with increasing doses of transgenic oocysts and challenged two weeks later with<jats:italic>E. maxima<\/jats:italic>to quantify the effect of vaccination on parasite replication, local IFN-\u03b3 and IL-10 responses (300 oocysts), as well as impacts on intestinal lesions and body weight gain (10,000 oocysts).<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Vaccination of chickens with<jats:italic>E. tenella<\/jats:italic>expressing<jats:italic>Em<\/jats:italic>AMA1, or admixtures of<jats:italic>E. tenella<\/jats:italic>expressing<jats:italic>Em<\/jats:italic>AMA1 or<jats:italic>Em<\/jats:italic>IMP1, was safe and induced partial protection against challenge as measured by<jats:italic>E. maxima<\/jats:italic>replication and severity of pathology. Higher levels of protection were observed when both antigens were delivered and was associated with a partial modification of local immune responses against<jats:italic>E. maxima<\/jats:italic>, which we hypothesise resulted in more rapid immune recognition of the challenge parasites.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>This study offers prospects for future development of multivalent anticoccidial vaccines for commercial chickens. Efforts should now be focused on the discovery of additional antigens for incorporation into such vaccines.<\/jats:p><\/jats:sec>","DOI":"10.1186\/s13071-020-04210-2","type":"journal-article","created":{"date-parts":[[2020,7,10]],"date-time":"2020-07-10T07:03:42Z","timestamp":1594364622000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":32,"title":["Vaccination with transgenic Eimeria tenella expressing Eimeria maxima AMA1 and IMP1 confers partial protection against high-level E. maxima challenge in a broiler model of coccidiosis"],"prefix":"10.1186","volume":"13","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-8454-5654","authenticated-orcid":false,"given":"Iv\u00e1n","family":"Pastor-Fern\u00e1ndez","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sungwon","family":"Kim","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Virginia","family":"Marug\u00e1n-Hern\u00e1ndez","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Francesca","family":"Soutter","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fiona M.","family":"Tomley","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Damer P.","family":"Blake","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2020,7,10]]},"reference":[{"key":"4210_CR1","doi-asserted-by":"crossref","first-page":"341","DOI":"10.1016\/S0020-7519(98)00212-4","volume":"29","author":"RB Williams","year":"1999","unstructured":"Williams RB, Carlyle WW, Bond DR, Brown IA. 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All animal studies and protocols were approved by the Royal Veterinary College Animal Welfare & Ethical Review Body (London, UK) and the UK Government Home Office under specific project licence. The laboratory work involving genetic modified organisms (GMO) was conducted under authorisation GM9708.1, administered by the UK Health and Safety Executive.","order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Ethics approval and consent to participate"}},{"value":"Not applicable.","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Consent for publication"}},{"value":"The authors declare that they have no competing interests.","order":3,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing interests"}}],"article-number":"343"}}