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While in recent years, <jats:italic>G. duodenalis<\/jats:italic> genotype distribution patterns in humans have been intensely studied, there is still very little information available on the diversity of <jats:italic>Giardia<\/jats:italic> genotypes and sub-assemblages infecting people in Romania. In the present study, we investigated the genetic diversity of <jats:italic>Giardia duodenalis<\/jats:italic> in asymptomatic patients from Romania.<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Methods<\/jats:title>\n                <jats:p>Over an 11-month period, human feces from 7805 healthy adults were screened by microscopic analysis for <jats:italic>G. duodenalis<\/jats:italic> cysts during their obligatory periodic check-ups. DNA extraction was performed from microscopic-positive fecal samples, followed by multilocus sequence typing of four genetic loci of the ITS region, <jats:italic>gdh<\/jats:italic>, <jats:italic>tpi<\/jats:italic> and <jats:italic>bg<\/jats:italic> genes, followed by DNA sequencing and phylogenetic analysis. Statistical analysis was performed using EpiInfo 2000 software.<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Results<\/jats:title>\n                <jats:p>The prevalence of giardiasis in the present study was 0.42% (33\/7805). Twenty-three samples (76.67%) were successfully genotyped at each locus. The <jats:italic>bg<\/jats:italic> and <jats:italic>tpi<\/jats:italic> genes had the highest typing success rate (100%). The identified assemblages were assemblage A in 27 cases (subtypes A2 and A3), and B in 3 cases.<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Conclusions<\/jats:title>\n                <jats:p>To our knowledge, the present study is the first report of multilocus sequence typing of <jats:italic>G. duodenalis<\/jats:italic> isolated from humans in Romania. The present results may shed light on <jats:italic>G. duodenalis<\/jats:italic> infection in humans at a regional and national level, thus increasing awareness against this parasitic infection. <\/jats:p>\n              <\/jats:sec>","DOI":"10.1186\/s13071-020-04248-2","type":"journal-article","created":{"date-parts":[[2020,7,31]],"date-time":"2020-07-31T06:10:04Z","timestamp":1596175804000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":23,"title":["First multilocus sequence typing (MLST) of Giardia duodenalis isolates from humans in Romania"],"prefix":"10.1186","volume":"13","author":[{"given":"Carmen","family":"Costache","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4492-8383","authenticated-orcid":false,"given":"Zsuzsa","family":"Kalm\u00e1r","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hora\u021biu Alexandru","family":"Colosi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alina Mihaela","family":"Baciu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"R\u0103zvan Vlad","family":"Opri\u015f","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Adriana","family":"Gy\u00f6rke","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ioana Alina","family":"Colosi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2020,7,31]]},"reference":[{"key":"4248_CR1","doi-asserted-by":"publisher","first-page":"e1001923","DOI":"10.1371\/journal.pmed.1001923","volume":"12","author":"AH Havelaar","year":"2015","unstructured":"Havelaar AH, Kirk MD, Torgerson PR, Gibb HJ, Hald T, Lake RJ, et al. 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