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A clinical field study was conducted to demonstrate the efficacy and safety of an oral combination of sarolaner, moxidectin and pyrantel (Simparica Trio<jats:sup><jats:bold>\u00ae<\/jats:bold><\/jats:sup>) for the prevention of <jats:italic>A. vasorum<\/jats:italic> infection in dogs (prevention study). A survey study was conducted concurrently to determine the infection pressure in the same areas.<\/jats:p>\n<\/jats:sec><jats:sec>\n<jats:title>Methods<\/jats:title>\n<jats:p>Prevention and survey studies were both conducted at the same veterinary clinics in endemic hot spots for <jats:italic>A. vasorum<\/jats:italic> in Denmark and Italy. The prevention study was a randomized, placebo controlled, double masked study where 622 client-owned dogs were treated and tested at 30 days intervals for 10 months. In the survey study 1628 dogs that were at risk of infection and\/or were suspected to be infected were tested by fecal and\/or serological methods, and the percent of dogs positive for <jats:italic>A. vasorum<\/jats:italic> was calculated.<\/jats:p>\n<\/jats:sec><jats:sec>\n<jats:title>Results<\/jats:title>\n<jats:p>In the prevention study, there were no adverse events related to treatment with Simparica Trio<jats:sup><jats:bold>\u00ae<\/jats:bold><\/jats:sup>. Two placebo-treated animals became infected with <jats:italic>A. vasorum<\/jats:italic> during the 10-month study period, while none of the dogs in the combination product-treated group became infected. In the survey study, 12.2% of the study dogs were found positive to <jats:italic>A. vasorum<\/jats:italic>, indicating high exposure to the parasite during the period of the prevention study.<\/jats:p>\n<\/jats:sec><jats:sec>\n<jats:title>Conclusions<\/jats:title>\n<jats:p>Monthly oral treatment with the combination of sarolaner, moxidectin and pyrantel (Simparica Trio<jats:sup><jats:bold>\u00ae<\/jats:bold><\/jats:sup>) was 100% effective in the prevention of natural infection with <jats:italic>A. vasorum<\/jats:italic> in dogs in highly endemic areas. In endemic areas, <jats:italic>A. vasorum<\/jats:italic> occurrence in dogs at risk is considerable.<\/jats:p>\n<\/jats:sec>","DOI":"10.1186\/s13071-020-04262-4","type":"journal-article","created":{"date-parts":[[2020,7,29]],"date-time":"2020-07-29T17:02:53Z","timestamp":1596042173000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":13,"title":["Field safety and efficacy of an orally administered combination of sarolaner, moxidectin and pyrantel (Simparica Trio\u00ae) for the prevention of angiostrongylosis in dogs presented as veterinary patients"],"prefix":"10.1186","volume":"13","author":[{"given":"Csilla","family":"Becskei","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jakob L.","family":"Willesen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Manuela","family":"Schnyder","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Magda","family":"Wozniakiewicz","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nataliya","family":"Miroshnikova","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sean P.","family":"Mahabir","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2020,7,29]]},"reference":[{"key":"4262_CR1","doi-asserted-by":"publisher","first-page":"92","DOI":"10.1186\/s13567-014-0092-9","volume":"45","author":"HM Elsheikha","year":"2014","unstructured":"Elsheikha HM, Holmes SA, Wright I, Morgan ER, Lacher DW. 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