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Different animal species can serve as reservoirs and sources of dissemination of the disease, such as rodent species due their potential in transmitting zoonotic pathogens to humans and other animals. In the Canary Islands (Spain), <jats:italic>Cryptosporidium parvum<\/jats:italic> and <jats:italic>Cryptosporidium hominis<\/jats:italic> have been identified in patients with diarrhea. However, the occurrence of <jats:italic>Cryptosporidium<\/jats:italic> spp. in possible reservoirs in this archipelago remains unclear. Considering the zoonotic potential of these protozoans, the aim of the present study was to determine the presence of <jats:italic>Cryptosporidium<\/jats:italic> spp. in peridomestic wild rodents and the possible role of these mammals as a source of transmission of these protozoans in Canary Islands.<\/jats:p>\n<\/jats:sec><jats:sec>\n<jats:title>Methods<\/jats:title>\n<jats:p>A total of 179 rodents belonging to <jats:italic>Rattus rattus<\/jats:italic> and <jats:italic>Mus musculus domesticus<\/jats:italic> from four Canary Islands, La Palma, El Hierro, Tenerife and Lanzarote, were analyzed. Feces were screened for <jats:italic>Cryptosporidium<\/jats:italic> spp. by nested PCR of the <jats:italic>18S<\/jats:italic> ribosomal RNA fragment and the sequences used for phylogenetic analyses.<\/jats:p>\n<\/jats:sec><jats:sec>\n<jats:title>Results<\/jats:title>\n<jats:p><jats:italic>Cryptosporidium<\/jats:italic> spp. were found widely distributed with an overall prevalence of 12.30% in rodents (13.86% for <jats:italic>R. rattus<\/jats:italic> and 10.25% for <jats:italic>M. m. domesticus<\/jats:italic>). The overall prevalence by island was 19.60% for Tenerife, 7.14% for La Palma, 5.71% for El Hierro and 0% for Lanzarote. <jats:italic>Cryptosporidium tyzzeri<\/jats:italic>, <jats:italic>Cryptosporidium meleagridis<\/jats:italic>, <jats:italic>Cryptosporidium muris<\/jats:italic> and <jats:italic>Cryptosporidium<\/jats:italic> sp. rat genotype I and II\/III were successfully identified, in addition to two unidentified <jats:italic>Cryptosporidium<\/jats:italic> genotypes.<\/jats:p>\n<\/jats:sec><jats:sec>\n<jats:title>Conclusions<\/jats:title>\n<jats:p>This study contributes to the knowledge of the biodiversity and distribution of <jats:italic>Cryptosporidium<\/jats:italic> spp. in wild rodents from the Canary Islands, highlighting the presence of three zoonotic species, <jats:italic>C. tyzzeri<\/jats:italic>, <jats:italic>C. meleagridis<\/jats:italic> and <jats:italic>C. muris<\/jats:italic>, being the first detection of these three species in wild rodents in the Canary Islands and the first report of <jats:italic>C. meleagridis<\/jats:italic> in <jats:italic>R. rattus<\/jats:italic>. Given the results obtained in our study, future studies in non-sampled areas are required to better understand the epidemiology of these protozoans in wild rodents in the archipelago.<\/jats:p>\n<\/jats:sec>","DOI":"10.1186\/s13071-020-04330-9","type":"journal-article","created":{"date-parts":[[2020,9,4]],"date-time":"2020-09-04T11:02:57Z","timestamp":1599217377000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":34,"title":["Diversity of Cryptosporidium spp. in wild rodents from the Canary Islands, Spain"],"prefix":"10.1186","volume":"13","author":[{"given":"Katherine","family":"Garc\u00eda-Livia","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aar\u00f3n","family":"Mart\u00edn-Alonso","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pilar","family":"Foronda","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2020,9,4]]},"reference":[{"key":"4330_CR1","doi-asserted-by":"crossref","first-page":"1181","DOI":"10.1016\/j.ijpara.2005.07.009","volume":"35","author":"PR Hunter","year":"2005","unstructured":"Hunter PR, Thompson RA. 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