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While cats have not been found to be infected with the organism, significant percentages of dogs from Australia and Africa are infected, indicating that they may be important mammalian reservoirs. The objective of this study was to determine the presence of\n                      <jats:italic>R. felis<\/jats:italic>\n                      DNA in the blood of domestic dogs and cats in the USA.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      Three previously validated PCR assays for\n                      <jats:italic>R. felis<\/jats:italic>\n                      and DNA sequencing were performed on blood samples obtained from clinically ill domestic cats and dogs from 45 states (2008\u20132020) in the USA. The blood samples had been submitted for the diagnosis of various tick-borne diseases in dogs and feline infectious peritonitis virus, feline immunodeficiency virus, and\n                      <jats:italic>Bartonella<\/jats:italic>\n                      spp. in cats. Phylogenetic comparisons were performed on the\n                      <jats:italic>gltA<\/jats:italic>\n                      nucleotide sequences obtained in the study and those reported for\n                      <jats:italic>R. felis<\/jats:italic>\n                      and\n                      <jats:italic>R. felis<\/jats:italic>\n                      -like organisms.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      Low copy numbers of\n                      <jats:italic>R. felis<\/jats:italic>\n                      DNA (around 100\u00a0copies\/ml whole blood) were found in four cats (4\/752, 0.53%) and three dogs (3\/777, 0.39%). The very low levels of infection in clinically ill animals is consistent with\n                      <jats:italic>R. felis<\/jats:italic>\n                      being an unlikely cause of disease in naturally infected dogs and cats. The low copy numbers we found emphasize the requirement for very sensitive PCRs in prevalence studies.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>\n                      The low prevalence of naturally infected PCR-positive cats is further evidence that cats are unlikely to be important reservoirs of\n                      <jats:italic>R. felis<\/jats:italic>\n                      . Similarly, the low prevalence in dogs suggests they are not important reservoirs in the USA. Investigations should continue into the role other mammalian species may be playing in the epidemiology of\n                      <jats:italic>R. felis<\/jats:italic>\n                      infections.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Graphical Abstract<\/jats:title>\n                  <\/jats:sec>","DOI":"10.1186\/s13071-020-04464-w","type":"journal-article","created":{"date-parts":[[2020,11,18]],"date-time":"2020-11-18T01:03:32Z","timestamp":1605661412000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":15,"title":["Identification of Rickettsia felis DNA in the blood of domestic cats and dogs in the USA"],"prefix":"10.1186","volume":"13","author":[{"given":"Md Monirul","family":"Hoque","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Subarna","family":"Barua","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Patrick John","family":"Kelly","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kelly","family":"Chenoweth","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bernhard","family":"Kaltenboeck","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7874-8340","authenticated-orcid":false,"given":"Chengming","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2020,11,18]]},"reference":[{"key":"4464_CR1","doi-asserted-by":"publisher","first-page":"1007","DOI":"10.1089\/vbz.2010.0270","volume":"11","author":"SF Hii","year":"2011","unstructured":"Hii SF, Kopp SR, Abdad MY, Thompson MF, O\u2019Leary CA, Rees RL, et al. 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These de-identified samples that would be discarded can be utilized for research without CRRC\/IACUC approval.","order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Ethics approval and consent to participate"}},{"value":"Not applicable","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Consent for publication"}},{"value":"The authors declare that they have not competing interests.","order":3,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing interests"}}],"article-number":"581"}}