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Captive wildlife may be potential reservoirs for human infection with <jats:italic>Blastocystis<\/jats:italic> sp. The present study was performed to investigate the prevalence and subtype distribution of <jats:italic>Blastocystis<\/jats:italic> sp. in zoo animals in Sichuan Province, southwestern China.<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Methods<\/jats:title>\n                <jats:p>A total of 420 fresh fecal samples were collected from 54 captive wildlife species in four zoos in southwestern China between June 2017 and September 2019. The prevalence and subtype (ST) genetic characteristics of <jats:italic>Blastocystis<\/jats:italic> sp. were determined by PCR amplification of the barcode region of the <jats:italic>SSU<\/jats:italic> rRNA gene and phylogenetic analysis.<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Results<\/jats:title>\n                <jats:p>Overall, 15.7% (66\/420) of the animal samples and 20.7% (14\/54) of the species tested were shown to be infected with <jats:italic>Blastocystis<\/jats:italic> sp. The highest prevalence of <jats:italic>Blastocystis<\/jats:italic> sp. was found in Panzhihua Zoo (24.3%), which was significantly higher than that in Chengdu Zoo (6.9%), and Xichang Zoo (2.9%) (<jats:italic>P<\/jats:italic>\u2009&lt;\u20090.05). There are also significant differences in the prevalence of <jats:italic>Blastocystis<\/jats:italic> sp. among different species (<jats:italic>P<\/jats:italic>\u2009&lt;\u20090.05), and the highest of <jats:italic>Blastocystis<\/jats:italic> sp. prevalence was observed in white-cheeked gibbon, black great squirrel, and red giant flying squirrel (100%). Subtype analysis of <jats:italic>Blastocystis<\/jats:italic> sp. revealed nine subtypes, including six zoonotic STs (ST1-5, and ST8) and three animal-specific STs (ST10, ST14, and ST17), with ST17 as the predominant subtype (26\/66) in <jats:italic>Blastocystis<\/jats:italic> sp.-positive isolates.<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Conclusions<\/jats:title>\n                <jats:p>To our knowledge, this is the first report on the prevalence and subtype distribution of <jats:italic>Blastocystis<\/jats:italic> sp. among captive wildlife in zoos in southwestern China. This study highlights that these animals may serve as reservoirs for human <jats:italic>Blastocystis<\/jats:italic> sp. infections.<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Graphical Abstract<\/jats:title>\n                \n              <\/jats:sec>","DOI":"10.1186\/s13071-020-04515-2","type":"journal-article","created":{"date-parts":[[2021,1,6]],"date-time":"2021-01-06T18:03:54Z","timestamp":1609956234000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":42,"title":["First identification and molecular subtyping of Blastocystis sp. in zoo animals in southwestern China"],"prefix":"10.1186","volume":"14","author":[{"given":"Lei","family":"Deng","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jingxin","family":"Yao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shanyu","family":"Chen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tingmei","family":"He","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yijun","family":"Chai","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ziyao","family":"Zhou","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaogang","family":"Shi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Haifeng","family":"Liu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhijun","family":"Zhong","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hualin","family":"Fu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9898-5754","authenticated-orcid":false,"given":"Guangneng","family":"Peng","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2021,1,6]]},"reference":[{"key":"4515_CR1","doi-asserted-by":"publisher","first-page":"523","DOI":"10.1016\/j.pt.2013.08.006","volume":"29","author":"PD Scanlan","year":"2013","unstructured":"Scanlan PD, Stensvold CR. 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