{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,12]],"date-time":"2026-10-12T22:12:19Z","timestamp":1791843139964,"version":"4.3.4"},"reference-count":25,"publisher":"Springer Science and Business Media LLC","issue":"1","license":[{"start":{"date-parts":[[2020,1,23]],"date-time":"2020-01-23T00:00:00Z","timestamp":1579737600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2020,1,23]],"date-time":"2020-01-23T00:00:00Z","timestamp":1579737600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100009527","name":"Myndigheten f\u00f6r Samh\u00e4llsskydd och Beredskap","doi-asserted-by":"publisher","award":["2012-172"],"award-info":[{"award-number":["2012-172"]}],"id":[{"id":"10.13039\/100009527","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Mexican National Council of Science and Technology"}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Parasites Vectors"],"published-print":{"date-parts":[[2020,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec>\n<jats:title>Background<\/jats:title>\n<jats:p>Feline cryptosporidiosis is an increasing problem, especially in catteries. In humans, close contact with cats could be a potential source of infection although the risk of contracting cryptosporidiosis caused by <jats:italic>Cryptosporidium felis<\/jats:italic> is considered to be relatively low. Sequencing of the 60-kDa glycoprotein gene is a commonly used tool for investigation of the genetic diversity and transmission dynamics of <jats:italic>Cryptosporidium<\/jats:italic> species. However, until now the sequence of <jats:italic>gp60<\/jats:italic> from <jats:italic>C. felis<\/jats:italic> has not been available and genotyping has been limited to less discriminatory markers, such as <jats:italic>18S<\/jats:italic> rRNA, COWP and HSP70.<\/jats:p>\n<\/jats:sec><jats:sec>\n<jats:title>Methods<\/jats:title>\n<jats:p>We have identified the <jats:italic>gp60<\/jats:italic> orthologue within the genome sequence of <jats:italic>C. felis<\/jats:italic>, and used the sequence to design a nested PCR for subtyping purposes. A total of 128 clinical isolates of both feline and human origin, were used to evaluate the marker.<\/jats:p>\n<\/jats:sec><jats:sec>\n<jats:title>Results<\/jats:title>\n<jats:p>Sequence analysis revealed large variations between the different samples. The <jats:italic>C. felis gp60<\/jats:italic> lack the characteristic serine-tract found in many other cryptosporidian orthologues, instead it has an insertion of variable length (361\u2013742\u00a0nt). Also, two cases of suspected zoonotic transmission of <jats:italic>C. felis<\/jats:italic> between cats and humans were successfully confirmed.<\/jats:p>\n<\/jats:sec><jats:sec>\n<jats:title>Conclusions<\/jats:title>\n<jats:p>We have identified the <jats:italic>gp60<\/jats:italic> gene in <jats:italic>C. felis<\/jats:italic> and show how this highly variable marker can be used in epidemiological investigations.<\/jats:p>\n<\/jats:sec>","DOI":"10.1186\/s13071-020-3906-9","type":"journal-article","created":{"date-parts":[[2020,1,23]],"date-time":"2020-01-23T14:03:07Z","timestamp":1579788187000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":44,"title":["Development of a gp60-subtyping method for Cryptosporidium felis"],"prefix":"10.1186","volume":"13","author":[{"given":"Laura","family":"Rojas-Lopez","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kristin","family":"Elwin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rachel M.","family":"Chalmers","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Heidi L.","family":"Enemark","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jessica","family":"Beser","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Karin","family":"Troell","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2020,1,23]]},"reference":[{"key":"3906_CR1","doi-asserted-by":"publisher","first-page":"72","DOI":"10.1128\/CMR.17.1.72-97.2004","volume":"17","author":"L Xiao","year":"2004","unstructured":"Xiao L, Fayer R, Ryan U, Upton SJ. 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The DNA from oocysts collected in the UK were shared for public health monitoring and quality assurance purposes and ethical approval was not required. Feline samples were sent to the National Veterinary Institute, Uppsala, Sweden, for diagnostic purposes and ethical approval is not required.","order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Ethics approval and consent participate"}},{"value":"Not applicable.","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Consent for publication"}},{"value":"The authors declare that they have no competing interests.","order":3,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing interests"}}],"article-number":"39"}}