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Some strains of <jats:italic>Wolbachia<\/jats:italic>, when introduced into <jats:italic>Aedes aegypti<\/jats:italic> mosquitoes, reduce or block the replication of RNA viruses pathogenic to humans. The <jats:italic>w<\/jats:italic>AlbB strain of <jats:italic>Wolbachia<\/jats:italic> was originally isolated from <jats:italic>Aedes albopictus<\/jats:italic>, and when transinfected into <jats:italic>Ae. aegypti<\/jats:italic>, persists in mosquitoes under high temperature conditions longer than other strains. The utility of <jats:italic>w<\/jats:italic>AlbB to block a broad spectrum of RNA viruses has received limited attention. Here we test the ability of <jats:italic>w<\/jats:italic>AlbB to reduce or block the replication of a range of <jats:italic>Flavivirus<\/jats:italic> and <jats:italic>Alphavirus<\/jats:italic> species in cell culture.<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Methods<\/jats:title>\n                <jats:p>The C6\/36 mosquito cell line was stably infected with the <jats:italic>w<\/jats:italic>AlbB strain using the shell-vial technique. The replication of dengue, West Nile and three strains of Zika (genus <jats:italic>Flavivirus<\/jats:italic>), and Ross River, Barmah Forest and Sindbis (genus <jats:italic>Alphavirus<\/jats:italic>) viruses was compared in <jats:italic>w<\/jats:italic>AlbB-infected cells with <jats:italic>Wolbachia<\/jats:italic>-free controls. Infectious virus titres were determined using either immunofocus or plaque assays. A general linear model was used to test for significant differences in replication between flaviviruses and alphaviruses.<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Results<\/jats:title>\n                <jats:p>Titres of all viruses were significantly reduced in cell cultures infected with <jats:italic>w<\/jats:italic>AlbB versus <jats:italic>Wolbachia<\/jats:italic>-free controls. The magnitude of reduction in virus yields varied among virus species and, within species, also among the strains utilized.<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Conclusion<\/jats:title>\n                <jats:p>Our results suggest that <jats:italic>w<\/jats:italic>AlbB infection of arthropods could be used to reduce transmission of a wide range of pathogenic RNA viruses.<\/jats:p>\n              <\/jats:sec>","DOI":"10.1186\/s13071-020-3936-3","type":"journal-article","created":{"date-parts":[[2020,2,10]],"date-time":"2020-02-10T16:02:47Z","timestamp":1581350567000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":24,"title":["Wolbachia strain wAlbB blocks replication of flaviviruses and alphaviruses in mosquito cell culture"],"prefix":"10.1186","volume":"13","author":[{"given":"O\u2019mezie","family":"Ekwudu","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gregor J.","family":"Devine","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"John G.","family":"Aaskov","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8628-4216","authenticated-orcid":false,"given":"Francesca D.","family":"Frentiu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2020,2,10]]},"reference":[{"issue":"Suppl. 12","key":"3936_CR1","doi-asserted-by":"publisher","first-page":"S7","DOI":"10.1038\/nrmicro2460","volume":"8","author":"MG Guzman","year":"2010","unstructured":"Guzman MG, Halstead SB, Artsob H, Buchy P, Farrar J, Gubler DJ, et al. 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