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In each study, 10 purpose-bred Beagle or mixed-breed dogs were randomly allocated to one of two treatment groups based on pre-treatment host-suitability tick counts. Dogs were infested with approximately 50 (45\u201355) unfed adult ticks on Days -2, 5, 12, 19, 26 and 33. On Day 0, dogs received either a single oral dose of Simparica Trio\u2122 at the minimum label dose of 1.2 mg\/kg sarolaner, 24 \u00b5g\/kg moxidectin and 5 mg\/kg pyrantel (as pamoate salt) or placebo. Tick counts were conducted at 48 h post-treatment and after each subsequent weekly re-infestation for <jats:italic>A. maculatum<\/jats:italic>, <jats:italic>D. variabilis<\/jats:italic>, <jats:italic>I. scapularis<\/jats:italic> and <jats:italic>R. sanguineus<\/jats:italic> studies and at 48 hours or at 72 h post-treatment and after weekly re-infestation in the first and second <jats:italic>A. americanum<\/jats:italic> studies, respectively.<\/jats:p>\n<\/jats:sec><jats:sec>\n<jats:title>Results<\/jats:title>\n<jats:p>No treatment-related adverse reactions occurred in any study. In all studies, placebo-treated dogs maintained infestations throughout the entire study duration, and dogs treated with Simparica Trio\u2122 had significantly lower (<jats:italic>P<\/jats:italic> \u2264 0.0010) mean live tick counts than placebo-treated dogs at all time-points. Against <jats:italic>A. maculatum<\/jats:italic>, <jats:italic>D. variabilis<\/jats:italic>, <jats:italic>I. scapularis<\/jats:italic> and <jats:italic>R. sanguineus<\/jats:italic>, a single oral dose of Simparica Trio\u2122 evaluated at 48 h post-treatment provided \u2265 98.9% efficacy against existing infestations, and within 48 h of re-infestation efficacy was \u2265 90.4% through at least Day 28 (except for <jats:italic>R<\/jats:italic>. <jats:italic>sanguineus<\/jats:italic> on Day 14 in a single study with an efficacy of 89.7%). Against <jats:italic>A. americanum<\/jats:italic>, Simparica Trio\u2122 provided \u2265 99.4% efficacy at \u2264 72 h after treatment of existing infestations and maintained \u2265 98.4% efficacy at \u2264 72 h after re-infestation through at least Day 35.<\/jats:p>\n<\/jats:sec><jats:sec>\n<jats:title>Conclusions<\/jats:title>\n<jats:p>A single dose of Simparica Trio\u2122 administered orally at the minimum label dosage of 1.2 mg\/kg sarolaner, 24 \u00b5g\/kg moxidectin and 5 mg\/kg pyrantel provided treatment and control of the common tick species infesting dogs in the USA for at least one month.<\/jats:p>\n<\/jats:sec>","DOI":"10.1186\/s13071-020-3945-2","type":"journal-article","created":{"date-parts":[[2020,3,1]],"date-time":"2020-03-01T00:03:04Z","timestamp":1583020984000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":15,"title":["Efficacy of a novel orally administered combination product containing sarolaner, moxidectin and pyrantel (Simparica Trio\u2122) against induced infestations of five common tick species infesting dogs in the USA"],"prefix":"10.1186","volume":"13","author":[{"given":"Kristina","family":"Kryda","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sean P.","family":"Mahabir","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sara","family":"Chapin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Susan J.","family":"Holzmer","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Laurel","family":"Bowersock","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"William R.","family":"Everett","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"John","family":"Riner","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lori","family":"Carter","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David","family":"Young","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2020,3,1]]},"reference":[{"key":"3945_CR1","doi-asserted-by":"publisher","first-page":"1173","DOI":"10.1016\/j.cvsm.2009.07.001","volume":"39","author":"BL Blagburn","year":"2009","unstructured":"Blagburn BL, Dryden MW. 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