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Elimination of immature stages of these parasites before egg-laying will reduce environmental contamination and the risk of infection for both dogs and humans. Studies were conducted to evaluate the efficacy of a novel, oral chewable tablet containing sarolaner, moxidectin and pyrantel (Simparica Trio\u2122) against induced immature adult (L<jats:sub>5<\/jats:sub>) and adult <jats:italic>T. canis<\/jats:italic>, and adult <jats:italic>T. leonina<\/jats:italic> infections in dogs.<\/jats:p>\n<\/jats:sec><jats:sec>\n<jats:title>Methods<\/jats:title>\n<jats:p>Six negative-controlled, masked, randomized laboratory studies were conducted. Two studies each evaluated efficacy against immature adult (L<jats:sub>5<\/jats:sub>) <jats:italic>T. canis<\/jats:italic>, adult <jats:italic>T. canis<\/jats:italic>, and adult <jats:italic>T. leonina.<\/jats:italic> Sixteen to 40 dogs were included in each study. Dogs experimentally infected with the target parasite were dosed once on Day 0 with either placebo tablets or Simparica Trio\u2122 tablets to provide minimum dosages of 1.2\u00a0mg\/kg sarolaner, 24\u00a0\u00b5g\/kg moxidectin and 5.0\u00a0mg\/kg pyrantel (as pamoate salt). Efficacy was based on the number of worms recovered at necropsy 7\u201310\u00a0days after treatment compared to placebo control.<\/jats:p>\n<\/jats:sec><jats:sec>\n<jats:title>Results<\/jats:title>\n<jats:p>Based on geometric mean worm counts, efficacy of the sarolaner\u2009+\u2009moxidectin\u2009+\u2009pyrantel combination was \u2265\u200995.2% against immature adult <jats:italic>T. canis<\/jats:italic>, \u2265\u200997.3% against adult <jats:italic>T. canis<\/jats:italic>, and \u2265\u200989.7% against adult <jats:italic>T. leonina<\/jats:italic>. There were no treatment-related adverse events in any study.<\/jats:p>\n<\/jats:sec><jats:sec>\n<jats:title>Conclusions<\/jats:title>\n<jats:p>These studies confirm the efficacy of a single dose of a new oral chewable tablet containing sarolaner, moxidectin and pyrantel (Simparica Trio\u2122) against immature adult and adult <jats:italic>T. canis<\/jats:italic>, and adult <jats:italic>T. leonina<\/jats:italic> infections in dogs.<\/jats:p>\n<\/jats:sec>","DOI":"10.1186\/s13071-020-3950-5","type":"journal-article","created":{"date-parts":[[2020,3,1]],"date-time":"2020-03-01T00:03:04Z","timestamp":1583020984000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":16,"title":["Efficacy of a new oral chewable tablet containing sarolaner, moxidectin and pyrantel (Simparica Trio\u2122) against induced ascarid infections in dogs"],"prefix":"10.1186","volume":"13","author":[{"given":"Csilla","family":"Becskei","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kristina","family":"Kryda","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mirjan","family":"Thys","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Susan","family":"Holzmer","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Laurel","family":"Bowersock","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tiago","family":"Fernandes","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Leon","family":"Meyer","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Craig","family":"Reinemeyer","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sean P.","family":"Mahabir","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2020,3,1]]},"reference":[{"key":"3950_CR1","doi-asserted-by":"publisher","first-page":"398","DOI":"10.1016\/j.vetpar.2012.12.035","volume":"193","author":"PA Overgaauw","year":"2013","unstructured":"Overgaauw PA, Van Knapen F. 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CB, KK, MT, SH, LB, TF and SPM were employees of Zoetis. CR and LM were contracted study investigators.","order":3,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing interests"}}],"article-number":"71"}}